Sample Loading Buffers and Reagents

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Overview

Premixed sample buffers are available for numerous applications, including native PAGE, SDS-PAGE, peptide analyis, analytical IEF, nucleic acid sample preparation, and zymogram gel sample preparation.
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Description

Description

Sample Loading Buffers

Premixed loading buffers remove variables that cause lane-to-lane running anomalies. No preparation is required, saving valuable time. Bio-Rad premixed sample buffers are available for numerous applications, including native PAGE, SDS-PAGE, peptide analysis, analytical IEF, nucleic acid sample preparation (denaturing and nondenaturing), and zymogram gel sample preparation.

Bio-Rad electrophoresis-purity reagents are quality controlled to ensure reproducible results and are available as individual reagents or as premixed buffers. For more information, request bulletin 2317.

Premixed Electrophoresis Buffers

Simplify and standardize your sample loading with premixed sample buffers. The concentrated formulas allow these buffers to be used with both liquid and lyophilized samples. All premixed sample buffers are tested to ensure quality and consistency.

Ordering

Ordering

items

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1610737
30 ml, premixed protein sample buffer for SDS-PAGE
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1610738
30 ml, premixed protein sample buffer for native PAGE, contains 62.5 mM Tris-HCl, pH 6.8, 40% glycerol, 0.01% bromophenol blue
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1610739
30 ml, premixed protein sample buffer for peptide and small protein SDS-PAGE, contains 200 mM Tris-HCl, pH 6.8, 40% glycerol, 2% SDS, 0.04% Coomassie Blue G-250
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1610747
10 ml, premixed 4x Laemmli protein sample buffer for SDS-PAGE
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1610763
30 ml, protein sample buffer, 50% glycerol, for use in IEF with both liquid and lyophilized samples
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1610764
30 ml, protein sample buffer, 62.5 mM Tris-HCl, pH 6.8, 25% glycerol, 4% SDS, 0.01% bromophenol blue, for use in protease analysis
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(Discontinued) XT Sample Buffer
1610791
10 ml, 4x premixed protein sample buffer for use with all Criterion XT gels
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(Discontinued) XT Reducing Agent
1610792
1 ml, 20x reducing agent for use with all Criterion XT gels
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(Discontinued) Dithiothreitol (DTT)
1610610
1 g, dithiothreitol (DTT) reducing agent
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(Discontinued) Dithiothreitol (DTT)
1610611
5 g, dithiothreitol (DTT) reducing agent
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(Discontinued) 2-Mercaptoethanol
1610710
25 ml, ≥98% pure 2-mercaptoethanol (14.2 M)
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1632101
0.6 ml, 200 mM, reducing agent for use in protein sample preparation applications
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(Discontinued) EDTA
1610729
500 g, ≥99% pure EDTA (disodium ethylenediaminetetraacetate dihydrate) powder
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(Discontinued) Iodoacetamide
1632109
30 g, alkylating agent for use in protein sample preparation applications

Documents

Documents

Number Description Options
4006027
Instruction Manual, Native Sample Buffer, Rev F
4106111
Instruction Manual, Zymogram Sample Buffer, Rev B
4106115
Instruction Manual, TBE-Urea Sample Buffer, Rev B
4006030
Instruction Manual, Tricine Sample Buffer, Rev E
4006028
Instruction Manual, Laemmli Sample Buffer, Rev J
2317
Ready-to-Run Buffers and Solutions Brochure, Rev F