Precision Plus Protein™ WesternC™ Blotting Standards, 250 µl #1610376

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To support this effort, the company has implemented a discount policy that allows K&ndash;12 and college teaching laboratories to purchase kits, instruments, reagents, and other equipment at preferred prices. These discounted products are indicated by an EDU suffix (for example, 1660003EDU).</p> <p class="headerClassText">How do I apply for the education discount?</p> <p class="descClassText">If you are an educator at the K&ndash;12 or college level, contact your local <a href="/en-au/contact-us">Customer Service</a> office to establish an education account number.</p> </div> 0 /webroot/web/html EDU_price_overlay.html /webroot/web/html/EDU_price_overlay.html sandeep Edu Edu Price Overlay Edu Price Overlay Content 07/30/13 03:25 AM 07/30/37 05:00 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSE /LSE N 0 {"ecommerce":{"detail":{"products":{"price":"$175.00","name":"Precision Plus Protein&trade; WesternC&trade; Blotting Standards, 250 µl","variant":"BRC","id":"1610376","category":"BRC","brand":"BRC"}}},"event":"detail"} 1610376 09/21/09 12:03 PM en_US true internet/product_SKU 2019-05-04 19:23:46 /default/main/bio-rad/STAGING AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/161-0376_0921120327675.xml /default/main/bio-rad/WORKAREA/default jebrbkza 2018-02-01 00:22:43 true jebrbkza templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/161-0376_0921120327675.xml LSR N /default/main/bio-rad en 09/21/22 12:03 PM 1556001545639 2019-05-04 19:23:46 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/161-0376_0921120327675.xml 1610376-precision-plus-protein-westernc-blotting-standards-250-ul 1 1610376 Precision Plus Protein WesternC Standards Precision Plus Protein&trade; WesternC&trade; Blotting Standards, 250 µl 250 μl, pre-stained, <em>Strep</em>-tagged recombinant proteins (10–250 kD), including three pink reference bands (25, 50, 75 kD), 50 applications 250 μl, 10 blue-stained, Strep-tagged recombinant proteins (10–250 kD), including three pink reference bands (25, 50, 75 kD), 50 applications 250 μl, pre-stained, <em>Strep</em>-tagged recombinant proteins (10–250 kD), including three pink reference bands (25, 50, 75 kD), 50 applications /webroot/web/images/lsr/products/electrophoresis/sku_thumb/global/161-0376_tn.jpg /webroot/web/images/lsr/products/electrophoresis/sku_view/global/161-0376_view.jpg /webroot/web/images/lsr/products/electrophoresis/sku_feature/global/161-0376_feature.jpg Precision Plus Protein&trade; WesternC&trade; Standards ,VN RepEx Precision Plus Protein&trade; WesternC&trade; Standards 250 μl, pre-stained, <em>Strep</em>-tagged recombinant proteins (10–250 kD), including three pink reference bands (25, 50, 75 kD), 50 applications 161-0376, 1610376, precision, plus, protein, westernc, standards, standard, markers, marker, molecular, weight, mw, ladders, ladder, prestained, blue-stained, gel, electrophoresis, western, blotting, blot, fluorescence, detectionwestern 09/21/09 12:03 PM 09/21/22 12:03 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSR /LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl N 0 <p>Use Precision Plus Protein WesternC Standards as a combination standard for western blotting and fluorescence detection in SDS-PAGE. These standards contain ten <em>Strep</em>-tagged recombinant proteins (10&ndash;250 kD), including three pink reference bands (25, 50, and 75 kD), as well as unstained versions of the <em>Strep</em>-tagged recombinant proteins for molecular weight sizing after blot development.</p> <p><strong>Applications and Uses</strong></p> <ul> <li>Monitoring electrophoretic separation</li> <li>Monitoring blot transfer efficiency with easy orientation via multicolor standards</li> <li>Molecular weight sizing of proteins after blot development</li> <li>Fluorescent blot detection of prestained bands, with blue bands visualized in the red wavelength range and pink bands in the green wavelength range</li> </ul> <h2 class="descHeader">Packaging Options</h2> <table class="pd_table pd_gridlines" border="0"> <tbody> <tr> <td width="319" valign="top"><strong>Number of Applications (Volume)<br /></strong></td> <td style="text-align: center;" width="319" valign="top"><strong>Catalog Number</strong></td> </tr> <tr class="pd_colorbackground"> <td width="319" valign="top">50 (250 &mu;l)</td> <td style="text-align: center;" width="319" valign="top">1610376</td> </tr> <tr> <td width="319" valign="top">250 (5 x 250 &mu;l)</td> <td style="text-align: center;" width="319" valign="top"><a href="/en-us/sku/1610399-precision-plus-protein-westernc-blotting-standards-1-25-ml?ID=1610399">1610399 <br /></a></td> </tr> </tbody> </table> <br /> <p><strong>More Information</strong></p> <p>Loading buffer: 30% (w/v) glycerol, 2% SDS, 62.5 mM Tris, pH 6.8, 50 mM DTT, 5 mM EDTA, 0.02% NaN<sub>3</sub>, 0.01% bromophenol blue.</p> <p>Fluorescent detection: prepare a 1:10 dilution of the WesternC Standard and load 5 &micro;l per well. No conjugate is needed for fluorescent blot detection.</p> <h2 class="descHeader">Related Products</h2> <ul> <li>Precision Protein StrepTactin-HRP Conjugate, 300 &micro;l (<a href="/en-us/sku/1610380-precision-protein-streptactin-hrp-conjugate-300-mul?ID=1610380">1610380</a>)</li> <li>Precision Protein StrepTactin-HRP Conjugate, 125 &micro;l (<a href="/en-us/sku/1610381-precision-protein-streptactin-hrp-conjugate-125-mul?ID=1610381">1610381</a>)</li> <li>StrepTactin-AP Conjugate, 300 &micro;l (<a href="/en-us/sku/1610382-precision-protein-streptactin-ap-conjugate-300-mul?ID=1610382">1610382</a>)</li> </ul> <p>More Precision Plus Protein Standards:</p> <ul> <li><a href="/en-us/product/unstained-protein-standards?ID=ab429c02-ab15-4967-a7b9-1f78850fc4d3">Precision Plus Protein Unstained Standards</a></li> <li><a href="/en-us/product/prestained-protein-standards?ID=a7b0f9ce-e080-4b51-ab99-4cded66497c1">Precision Plus Protein Prestained Standards</a></li> <li><a href="/en-us/product/ief-2d-protein-standards">IEF and 2-D Electrophoresis Protein Standards</a></li> </ul> <h2 class="descHeader">Related Categories</h2> <ul> <li><a href="/en-us/category/protein-ladders-standards-markers">Protein Ladders and Standards</a></li> <li><a href="/en-us/category/protein-gels">Precast Protein Gels</a></li> <li><a href="/en-us/category/protein-electrophoresis-blotting">Protein Electrophoresis and Blotting</a></li> <li><a href="/en-us/category/western-blotting?ID=9324fd3c-6af4-4551-831e-8db6fa3f3452">Western Blotting</a></li> </ul> <h2 class="descHeader">Supporting Documents</h2> <ul> <li>Precision Plus Protein WesternC Standards <a href="/webroot/web/pdf/lsr/literature/10008761.pdf" target="_blank" rel="noopener noreferrer">Instruction Manual</a></li> <li>StrepTactin HRP-Conjugate and AP-Conjugate <a href="/webroot/web/pdf/lsr/literature/MS4110000.pdf" target="_blank" rel="noopener noreferrer">Instruction Manual</a></li> </ul> <h2 class="descHeader">Online Resources</h2> <ul> <li><a href="/en-us/applications-technologies/introduction-protein-electrophoresis?ID=LUSOVO47B">Protein Electrophoresis</a></li> <li><a href="/en-us/applications-technologies/introduction-western-blotting?ID=LUSPPAKG4">Western Blotting</a></li> </ul> /webroot/web/html/lsr/products/SKUs/autopopulated/ 161-0376_0921120327675_en.html /webroot/web/html/lsr/products/SKUs/autopopulated//161-0376_0921120327675_en.html MW size range, kD 1859 10&ndash;250 1 0 Stain type 2195 Combination of stained Dual Color and unstained 1 0 Volume, &micro;l 2321 250 1 0 Total number of applications 2274 50 1 0 Shelf life at &ndash;20&deg;C, months 2150 12 1 0 Loading volume on mini gels, &micro;l 1793 for chemiluminescent detection, 5. Precision Protein&trade; StrepTactin-HRP or -AP conjugate needed. 1 0 Loading volume on mini gels, &micro;l 1793 for fluorescent blot detection, 5 of 1:10 dilution 1 0 <b>Application Type</b> 1130 &nbsp; 0 0 Monitoring electrophoretic separation for SDS-PAGE gels 2733 Yes 0 0 Molecular weight sizing on SDS-PAGE gels 2734 Yes 0 0 Monitoring blot transfer efficiency 2735 Yes 0 0 Fluorescence blots 1649 Yes 0 0 Molecular weight sizing on 2736 Blots 0 0 <b>Loading Volume on Mini Gels</b> 1142 &nbsp; 0 0 For Coomassie staining, &micro;l 1658 N/A 0 0 For stain-free gels, &micro;l 2737 N/A 0 0 For silver staining, &micro;l 2738 N/A 0 0 For SYPRO Ruby staining, &micro;l 2739 N/A 0 0 For blot development, &micro;l 1657 N/A 0 0 StrepTactin conjugate working dilutions for colorimetric blot development 2218 N/A 0 0 StrepTactin conjugate working dilutions for chemiluminescence blot development 2217 N/A 0 0 4561083 /templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/456-1083_1260291779.xml 1706404 /templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/170-6404_0921120401756.xml 1705060 /templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/170-5060_1343333743.xml false $175.00 false $ null true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1610376&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1610376&CartQuote=QT&quantity= 1205 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1610376&AddToDefaultWishList=true&quantity= 41105300 175.00 SKU detail page 161-0376-MSDS Precision Plus Protein Western-C Standards Korea Korean /webroot/web/pdf/WWMSDS/LSGC/ROK/ROK_KOR_1610376.pdf Belgium French 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250 μl, pre-stained, Strep-tagged recombinant proteins (10–250 kD), including three pink reference bands (25, 50, 75 kD), 50 applications

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To support this effort, the company has implemented a discount policy that allows K&ndash;12 and college teaching laboratories to purchase kits, instruments, reagents, and other equipment at preferred prices. These discounted products are indicated by an EDU suffix (for example, 1660003EDU).</p> <p class="headerClassText">How do I apply for the education discount?</p> <p class="descClassText">If you are an educator at the K&ndash;12 or college level, contact your local <a href="/en-au/contact-us">Customer Service</a> office to establish an education account number.</p> </div> 0 /webroot/web/html EDU_price_overlay.html /webroot/web/html/EDU_price_overlay.html sandeep Edu Edu Price Overlay Edu Price Overlay Content 07/30/13 03:25 AM 07/30/37 05:00 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSE /LSE N 0 {"ecommerce":{"detail":{"products":{"price":"$175.00","name":"Precision Plus Protein&trade; WesternC&trade; Blotting Standards, 250 µl","variant":"BRC","id":"1610376","category":"BRC","brand":"BRC"}}},"event":"detail"} 1610376 09/21/09 12:03 PM en_US true internet/product_SKU 2019-05-04 19:23:46 /default/main/bio-rad/STAGING AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/161-0376_0921120327675.xml /default/main/bio-rad/WORKAREA/default jebrbkza 2018-02-01 00:22:43 true jebrbkza templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/161-0376_0921120327675.xml LSR N /default/main/bio-rad en 09/21/22 12:03 PM 1556001545639 2019-05-04 19:23:46 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/161-0376_0921120327675.xml 1610376-precision-plus-protein-westernc-blotting-standards-250-ul 1 1610376 Precision Plus Protein WesternC Standards Precision Plus Protein&trade; WesternC&trade; Blotting Standards, 250 µl 250 μl, pre-stained, <em>Strep</em>-tagged recombinant proteins (10–250 kD), including three pink reference bands (25, 50, 75 kD), 50 applications 250 μl, 10 blue-stained, Strep-tagged recombinant proteins (10–250 kD), including three pink reference bands (25, 50, 75 kD), 50 applications 250 μl, pre-stained, <em>Strep</em>-tagged recombinant proteins (10–250 kD), including three pink reference bands (25, 50, 75 kD), 50 applications /webroot/web/images/lsr/products/electrophoresis/sku_thumb/global/161-0376_tn.jpg /webroot/web/images/lsr/products/electrophoresis/sku_view/global/161-0376_view.jpg /webroot/web/images/lsr/products/electrophoresis/sku_feature/global/161-0376_feature.jpg Precision Plus Protein&trade; WesternC&trade; Standards ,VN RepEx Precision Plus Protein&trade; WesternC&trade; Standards 250 μl, pre-stained, <em>Strep</em>-tagged recombinant proteins (10–250 kD), including three pink reference bands (25, 50, 75 kD), 50 applications 161-0376, 1610376, precision, plus, protein, westernc, standards, standard, markers, marker, molecular, weight, mw, ladders, ladder, prestained, blue-stained, gel, electrophoresis, western, blotting, blot, fluorescence, detectionwestern 09/21/09 12:03 PM 09/21/22 12:03 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSR /LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl N 0 <p>Use Precision Plus Protein WesternC Standards as a combination standard for western blotting and fluorescence detection in SDS-PAGE. These standards contain ten <em>Strep</em>-tagged recombinant proteins (10&ndash;250 kD), including three pink reference bands (25, 50, and 75 kD), as well as unstained versions of the <em>Strep</em>-tagged recombinant proteins for molecular weight sizing after blot development.</p> <p><strong>Applications and Uses</strong></p> <ul> <li>Monitoring electrophoretic separation</li> <li>Monitoring blot transfer efficiency with easy orientation via multicolor standards</li> <li>Molecular weight sizing of proteins after blot development</li> <li>Fluorescent blot detection of prestained bands, with blue bands visualized in the red wavelength range and pink bands in the green wavelength range</li> </ul> <h2 class="descHeader">Packaging Options</h2> <table class="pd_table pd_gridlines" border="0"> <tbody> <tr> <td width="319" valign="top"><strong>Number of Applications (Volume)<br /></strong></td> <td style="text-align: center;" width="319" valign="top"><strong>Catalog Number</strong></td> </tr> <tr class="pd_colorbackground"> <td width="319" valign="top">50 (250 &mu;l)</td> <td style="text-align: center;" width="319" valign="top">1610376</td> </tr> <tr> <td width="319" valign="top">250 (5 x 250 &mu;l)</td> <td style="text-align: center;" width="319" valign="top"><a href="/en-us/sku/1610399-precision-plus-protein-westernc-blotting-standards-1-25-ml?ID=1610399">1610399 <br /></a></td> </tr> </tbody> </table> <br /> <p><strong>More Information</strong></p> <p>Loading buffer: 30% (w/v) glycerol, 2% SDS, 62.5 mM Tris, pH 6.8, 50 mM DTT, 5 mM EDTA, 0.02% NaN<sub>3</sub>, 0.01% bromophenol blue.</p> <p>Fluorescent detection: prepare a 1:10 dilution of the WesternC Standard and load 5 &micro;l per well. No conjugate is needed for fluorescent blot detection.</p> <h2 class="descHeader">Related Products</h2> <ul> <li>Precision Protein StrepTactin-HRP Conjugate, 300 &micro;l (<a href="/en-us/sku/1610380-precision-protein-streptactin-hrp-conjugate-300-mul?ID=1610380">1610380</a>)</li> <li>Precision Protein StrepTactin-HRP Conjugate, 125 &micro;l (<a href="/en-us/sku/1610381-precision-protein-streptactin-hrp-conjugate-125-mul?ID=1610381">1610381</a>)</li> <li>StrepTactin-AP Conjugate, 300 &micro;l (<a href="/en-us/sku/1610382-precision-protein-streptactin-ap-conjugate-300-mul?ID=1610382">1610382</a>)</li> </ul> <p>More Precision Plus Protein Standards:</p> <ul> <li><a href="/en-us/product/unstained-protein-standards?ID=ab429c02-ab15-4967-a7b9-1f78850fc4d3">Precision Plus Protein Unstained Standards</a></li> <li><a href="/en-us/product/prestained-protein-standards?ID=a7b0f9ce-e080-4b51-ab99-4cded66497c1">Precision Plus Protein Prestained Standards</a></li> <li><a href="/en-us/product/ief-2d-protein-standards">IEF and 2-D Electrophoresis Protein Standards</a></li> </ul> <h2 class="descHeader">Related Categories</h2> <ul> <li><a href="/en-us/category/protein-ladders-standards-markers">Protein Ladders and Standards</a></li> <li><a href="/en-us/category/protein-gels">Precast Protein Gels</a></li> <li><a href="/en-us/category/protein-electrophoresis-blotting">Protein Electrophoresis and Blotting</a></li> <li><a href="/en-us/category/western-blotting?ID=9324fd3c-6af4-4551-831e-8db6fa3f3452">Western Blotting</a></li> </ul> <h2 class="descHeader">Supporting Documents</h2> <ul> <li>Precision Plus Protein WesternC Standards <a href="/webroot/web/pdf/lsr/literature/10008761.pdf" target="_blank" rel="noopener noreferrer">Instruction Manual</a></li> <li>StrepTactin HRP-Conjugate and AP-Conjugate <a href="/webroot/web/pdf/lsr/literature/MS4110000.pdf" target="_blank" rel="noopener noreferrer">Instruction Manual</a></li> </ul> <h2 class="descHeader">Online Resources</h2> <ul> <li><a href="/en-us/applications-technologies/introduction-protein-electrophoresis?ID=LUSOVO47B">Protein Electrophoresis</a></li> <li><a href="/en-us/applications-technologies/introduction-western-blotting?ID=LUSPPAKG4">Western Blotting</a></li> </ul> /webroot/web/html/lsr/products/SKUs/autopopulated/ 161-0376_0921120327675_en.html /webroot/web/html/lsr/products/SKUs/autopopulated//161-0376_0921120327675_en.html MW size range, kD 1859 10&ndash;250 1 0 Stain type 2195 Combination of stained Dual Color and unstained 1 0 Volume, &micro;l 2321 250 1 0 Total number of applications 2274 50 1 0 Shelf life at &ndash;20&deg;C, months 2150 12 1 0 Loading volume on mini gels, &micro;l 1793 for chemiluminescent detection, 5. Precision Protein&trade; StrepTactin-HRP or -AP conjugate needed. 1 0 Loading volume on mini gels, &micro;l 1793 for fluorescent blot detection, 5 of 1:10 dilution 1 0 <b>Application Type</b> 1130 &nbsp; 0 0 Monitoring electrophoretic separation for SDS-PAGE gels 2733 Yes 0 0 Molecular weight sizing on SDS-PAGE gels 2734 Yes 0 0 Monitoring blot transfer efficiency 2735 Yes 0 0 Fluorescence blots 1649 Yes 0 0 Molecular weight sizing on 2736 Blots 0 0 <b>Loading Volume on Mini Gels</b> 1142 &nbsp; 0 0 For Coomassie staining, &micro;l 1658 N/A 0 0 For stain-free gels, &micro;l 2737 N/A 0 0 For silver staining, &micro;l 2738 N/A 0 0 For SYPRO Ruby staining, &micro;l 2739 N/A 0 0 For blot development, &micro;l 1657 N/A 0 0 StrepTactin conjugate working dilutions for colorimetric blot development 2218 N/A 0 0 StrepTactin conjugate working dilutions for chemiluminescence blot development 2217 N/A 0 0 4561083 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Use Precision Plus Protein WesternC Standards as a combination standard for western blotting and fluorescence detection in SDS-PAGE. These standards contain ten Strep-tagged recombinant proteins (10–250 kD), including three pink reference bands (25, 50, and 75 kD), as well as unstained versions of the Strep-tagged recombinant proteins for molecular weight sizing after blot development.

Applications and Uses

  • Monitoring electrophoretic separation
  • Monitoring blot transfer efficiency with easy orientation via multicolor standards
  • Molecular weight sizing of proteins after blot development
  • Fluorescent blot detection of prestained bands, with blue bands visualized in the red wavelength range and pink bands in the green wavelength range

Packaging Options

Number of Applications (Volume)
Catalog Number
50 (250 μl) 1610376
250 (5 x 250 μl) 1610399

More Information

Loading buffer: 30% (w/v) glycerol, 2% SDS, 62.5 mM Tris, pH 6.8, 50 mM DTT, 5 mM EDTA, 0.02% NaN3, 0.01% bromophenol blue.

Fluorescent detection: prepare a 1:10 dilution of the WesternC Standard and load 5 µl per well. No conjugate is needed for fluorescent blot detection.

Related Products

  • Precision Protein StrepTactin-HRP Conjugate, 300 µl (1610380)
  • Precision Protein StrepTactin-HRP Conjugate, 125 µl (1610381)
  • StrepTactin-AP Conjugate, 300 µl (1610382)

More Precision Plus Protein Standards:

Related Categories

Supporting Documents

Online Resources

MW size range, kD 10–250
Stain type Combination of stained Dual Color and unstained
Volume, µl 250
Total number of applications 50
Shelf life at –20°C, months 12
Loading volume on mini gels, µl for chemiluminescent detection, 5. Precision Protein™ StrepTactin-HRP or -AP conjugate needed.
Loading volume on mini gels, µl for fluorescent blot detection, 5 of 1:10 dilution
Application Type  
Monitoring electrophoretic separation for SDS-PAGE gels Yes
Molecular weight sizing on SDS-PAGE gels Yes
Monitoring blot transfer efficiency Yes
Fluorescence blots Yes
Molecular weight sizing on Blots
Loading Volume on Mini Gels  
For Coomassie staining, µl N/A
For stain-free gels, µl N/A
For silver staining, µl N/A
For SYPRO Ruby staining, µl N/A
For blot development, µl N/A
StrepTactin conjugate working dilutions for colorimetric blot development N/A
StrepTactin conjugate working dilutions for chemiluminescence blot development N/A
Available Translation
Precision Plus Protein Western-C Standards  (SDS)gene

Bulletin# 161-0376-MSDS

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