Description
Description
Our ddPCR Supermix for Residual DNA Quantification is a 2x concentrated, ready-to-use digital pcr master mix optimized to deliver maximum PCR efficiency, specificity, and sensitivity for direct quantification of residual host cell DNA (HCD) with the Droplet Digital PCR (ddPCR) System.
Key Benefits
- Highly precise and sensitive method for direct quantification of residual host cell DNA
- No DNA purification required
- Free of detectable E. coli, CHO, mouse, human, and yeast DNA
- Contains all components required for hydrolysis probe–based ddPCR assays except primers, probe(s), and template
- Limits nonspecific PCR amplification
- Hot-start polymerase keeps enzyme inactive during sample droplet partitioning
Applications and Uses
- Absolute quantification of residual HCD
- Environmental monitoring
- Food testing
Packaging Options
| Number of 20 µl Reactions | Supermix Volume, ml | Catalog Number |
|---|---|---|
| 200 | 2 x 1 | 1864037 |
| 500 | 5 x 1 | 1864038 |
| 2,500 | 5 x 5 | 1864039 |
| 5,000 | 10 x 5 | 1864040 |
Additional Reagents
Bio-Rad offers additional consumables for completing your digital PCR workflow:
- Digital PCR consumables
- PrimePCR ddPCR Assays
Additional Equipment
This digital PCR supermix requires the use of one or more of the following instruments and accessories:
- Automated Droplet Generator
- QX200 Droplet Generator
- QX200 Droplet Reader
- PX1 PCR Plate Sealer
- PTC Tempo Deepwell Thermal Cycler or C1000 Touch Thermal Cycler with 96–Deep Well Reaction Module
More Information
This ddPCR Supermix for Residual DNA Quantification is optimized for use with Droplet Generation Oil for Probes on the QX200 Droplet Digital PCR System and QX200 AutoDG Droplet Digital System.
Specifications
Specifications
Ordering
Ordering
items
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2 ml (2 x 1 ml), 2x supermix, for direct quantification of residual host cell DNA in the QX600/QX200 Droplet Digital™ PCR Systems
5 ml (5 x 1 ml), 2x supermix, for direct quantification of residual host cell DNA in the QX600/QX200 Droplet Digital™ PCR Systems
50 ml (10 x 5 ml), 2x supermix, for direct quantification of residual host cell DNA in the QX600/QX200 Droplet Digital™ PCR Systems
25 ml (5 x 5 ml), 2x supermix, for direct quantification of residual host cell DNA in the QX600/QX200 Droplet Digital™ PCR Systems
Consumables
Consumables
70 ml (10 x 7 ml), oil for use with droplet generator in the QX600/QX200 Droplet Digital™ PCR Systems
2 L (2 x 1 L), oil for use with droplet reader in the QX600/QX200 Droplet Digital™ PCR Systems
Pkg of 24, cartridges for use with the QX200 Droplet Generator
Pkg of 24, gaskets for use with QX200 Droplet Generator
5 pkg of 24 DG8 Cartridges, 5 pkg of 24 DG8 Gaskets, for use with the QX200 Droplet Generator
Equipment
Equipment
QX200 AutoDG Droplet Digital PCR System enables and automates up to 2-color multiplexing for up to 96-samples per run providing clear discrimination of multiple targets. System includes Automated Droplet Generator, QX200 Droplet Reader, computer, and QX Manager Software, Standard Edition.
Droplet Digital PCR System, includes droplet generator, droplet reader, computer, software, associated component consumables, for EvaGreen or probe-based digital PCR applications
AutoDG Instrument for automated droplet generation of up to 96 samples, for use with the QX600/QX200 Droplet Digital PCR Systems
Droplet generator for EvaGreen or probe-based digital PCR applications, for use with QX200™ Droplet Digital™ PCR System
Droplet reader for EvaGreen or probe-based digital PCR applications, for use with the QX200 Droplet Digital™ System
Modular thermal cycler platform, includes C1000 Touch Thermal Cycler chassis, 96–deep well reaction module, USB flash drive
