Overview
50 ml (10 x 5 ml), 2x supermix, for direct quantification of residual host cell DNA in the QX600/QX200 Droplet Digital™ PCR Systems
Safety Data Sheet
Description
Description
Use this digital PCR supermix for residual host cell DNA (HCD) quantification to achieve maximum PCR efficiency, specificity, and sensitivity.
This 2x concentrated, ready-to-use mix has been optimized for use with Droplet Digital™ PCR (ddPCR™) Systems.
Key Benefits
- Highly precise and sensitive method for direct quantification of residual host cell DNA
- No DNA purification required
- Free of detectable E. coli, CHO, mouse, human, and yeast DNA
- Contains all components required for hydrolysis probe–based ddPCR except primers, probe(s), and template
- Limits nonspecific PCR amplification
- Hot-start polymerase keeps enzyme inactive during sample droplet partitioning
Applications and Uses
- Absolute quantification of residual HCD
- Environmental monitoring
- Food testing
Packaging Options
| Number of 20 µl Reactions | Supermix Volume, ml | Catalog Number |
| 200 | 2 x 1 | 1864037 |
| 500 | 5 x 1 | 1864038 |
| 2,500 | 5 x 5 | 1864039 |
| 5,000 | 10 x 5 | 1864040 |
Additional Reagents
Bio-Rad offers additional consumables for completing your digital PCR workflow:
- Digital PCR consumables
- PrimePCR™ ddPCR™ Assays
Additional Equipment
This digital PCR supermix requires the use of one or more of the following instruments and accessories:
- QX600 AutoDG Droplet Digital PCR System
- QX600 Droplet Digital PCR System
- Automated Droplet Generator
- QX200™ Droplet Generator
- QX200™ Droplet Reader
- PX1™ PCR Plate Sealer
- C1000 Touch™ Thermal Cycler with 96–Deep Well Reaction Module
Related Products
Bio-Rad offers additional digital PCR supermixes including:
- ddPCR™ Supermix for Probes (No dUTP)
- ddPCR™ Supermix for Probes
- QX200™ ddPCR™ EvaGreen Supermix
- One-Step RT-ddPCR Kit for Probes
- Digital PCR Library Quantification Kits
More Information
This ddPCR Supermix for Residual DNA Quantification is optimized for use with Droplet Generation Oil for Probes on the QX600 or QX200™ Droplet Digital™ PCR System and QX600 or QX200™ AutoDG™ Droplet Digital™ System.