Automated Droplet Generator #1864101

The AutoDG Instrument partitions each sample in a 96-well PCR plate into ~20,000 uniform nanoliter-sized droplets in which nucleic acid molecules are randomly distributed. Every droplet serves as an individual PCR reaction in Bio-Rad’s Droplet Digital (ddPCR) PCR System. After partitioning, the plate is sealed with the PX1 Plate Sealer, and PCR is run to end point in a thermal cycler. The droplets in each sample can then be analyzed using a QX600 or QX200 Droplet Reader.

• Eliminates user-to-user variability — creates more consistent droplet counts across users and plates, enabling you to fully exploit the precision of your ddPCR System
• Minimizes hands-on time — takes less than 5 minutes to load the instrument, and droplet generation requires no supervision

Features and Benefits of the Automated Droplet Generator

Bio-Rad’s Automated Droplet Generator simplifies ddPCR workflow and makes digital PCR scalable and flexible:

• Guides setup and loading with large, color touch-screen interface and LED lighting
• Generates droplets for 96 ddPCR reactions in under 45 minutes
• One AutoDG Instrument can generate sufficient droplets to keep 4–5 droplet readers running continuously
• Provides operational flexibility:
  • User-definable plate layout with increments of 8 wells
  • HEPA-filtered enclosure reduces the possibility of contamination and allows the system to be placed in any lab environment
  • Compatible with both QX600 and QX200 ddPCR workflows

Learn more about the applications of the QX600 AutoDG Droplet Digital PCR System and the QX200 AutoDG Droplet Digital PCR System.

Required Instruments

The Automated Droplet Generator requires the use of the following Bio-Rad instruments:

• QX600 Droplet Reader
• QX200 Droplet Reader
• PTC Tempo Deepwell Thermal Cycler or C1000 Touch Thermal Cycler with 96–Deep Well Reaction Module
• PX1 PCR Plate Sealer

Required Consumables

• DG32 Automated Droplet Generator Cartridges for the AutoDG System
• Automated Droplet Generation Oil for Probes
• Automated Droplet Generation Oil for EvaGreen
• Pipet Tips for the AutoDG System
• Pipet Tip Waste Bin for the AutoDG System
• Droplet Reader Oil

ddPCR Reagents for EvaGreen or TaqMan Hydrolysis Probe-Based Applications

• PrimePCR ddPCR Assays
• ddPCR Supermixes for Probes (no dUTP)
• QX200 ddPCR EvaGreen Supermix
• ddPCR Supermix for Probes
• One-Step RT-ddPCR Kit for Probes

Supporting Documents and Resources

QX200 AutoDG Droplet Digital PCR System Instruction Manual

Download the Droplet Digital PCR Applications Guide for in-depth information about setting up experiments for applications including: Copy number variation analysis Rare mutation and sequence detection Gene expression Next-generation sequencing library analysis