ddPCR™ Supermix for Residual DNA Quantification #1864037

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Overview

2 ml (2 x 1 ml), 2x supermix, for direct quantification of residual host cell DNA in the QX200™AutoDG™/ QX200™/QX100™ Droplet Digital™ PCR Systems
Safety Data Sheet

Safety Data Sheet

Description

Description

Use this digital PCR supermix for residual host cell DNA (HCD) quantification to achieve maximum PCR efficiency, specificity, and sensitivity.

This 2x concentrated, ready-to-use mix has been optimized for use with Droplet Digital™ PCR (ddPCR™) Systems.

Key Benefits

  • Highly precise and sensitive method for direct quantification of residual host cell DNA
  • No DNA purification required
  • Free of detectable E. coli, CHO, mouse, human, and yeast DNA
  • Contains all components required for hydrolysis probe–based ddPCR except primers, probe(s), and template
  • Limits nonspecific PCR amplification
  • Hot-start polymerase keeps enzyme inactive during sample droplet partitioning

Applications and Uses

  • Absolute quantification of residual HCD
  • Environmental monitoring
  • Food testing

Packaging Options

Number of 20 µl Reactions Supermix Volume, ml Catalog Number
200 2 x 1 1864037
500 5 x 1 1864038
2,500 5 x 5 1864039
5,000 10 x 5
1864040

Additional Reagents

Bio-Rad offers additional consumables for completing your digital PCR workflow:

Additional Equipment

This digital PCR supermix requires the use of one or more of the following instruments and accessories:

Related Products

Bio-Rad offers additional digital PCR supermixes including:

More Information

This ddPCR Supermix for Residual DNA Quantification is optimized for use with Droplet Generation Oil for Probes on the QX200™ Droplet Digital™ PCR System and QX200™ AutoDG™ Droplet Digital™ System.

Specifications

Specifications

Storage at –20°C
Up to 24 months (refer to expiration date)
Storage at 4°C (after thawing)
Up to 2 weeks
Template compatibility
cDNA, genomic DNA, plasmid DNA