Genomics Learning Center

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The Genomics Learning Center is a knowledge hub where you can find both general information on a range of genomics technologies and advanced solutions for PCR, qPCR/RT-PCR, digital PCR, and next-generation sequencing (NGS). The field of genomics research has grown incredibly and is constantly evolving, with new technologies pushing the boundaries of the study of genes and gene function. Whether you are an experienced genomics researcher or new to the field, it is critical to stay informed about the latest applications and techniques so you can design your experiments with confidence and drive your research forward.

Identification of Genomic Targets

Next-generation sequencing (NGS) and advanced PCR methods have revolutionized genomics and made sequencing accessible to a wide range of research applications. New technologies continue to advance long-range, direct, and single-cell sequencing, while new library preparation and nucleic acid quantification solutions help optimize workflows. Topics here include standard and emerging NGS methodologies for sequencing DNA and RNA targets plus advanced digital PCR tools for a range of genomics applications.

Latest Methods for the Identification of Genomic Targets

 

Quantification of Genomic Targets

PCR technology remains vital to genomic workflows, with new high-performance digital PCR systems offering vast improvements in accuracy and reproducibility. Digital PCR systems like droplet digital PCR (ddPCR) allow ultrasensitive and absolute nucleic acid quantification. While real-time PCR (qPCR) is often used for target detection, these droplet digital PCR systems provide exceptional sensitivity in detection of low abundant targets or complex targets, allelic variants (SNPs), or subtle changes in target levels. Topics here include the application of digital PCR technology to gene expression analysis, CNV analysis, and single-cell studies.

Latest Methods for the Quantification of Genomic Targets

 

Understanding Gene Function

The ultimate goal of genomics is to understand the function of genes in the context of health and disease. This is typically achieved by mutating or omitting the gene in question and analyzing the downstream cellular effects. CRISPR technology has greatly expanded our tool kit for targeted gene editing and introduced opportunities for novel gene therapies. Advancements in NGS are also allowing scientists to explore more of the non-coding transcriptome and revealing the diverse roles of non-coding RNAs. This section will explore topics in functional genomics, including genome editing, non-coding RNAs, and RNA-Seq data analysis.

Latest Methods for Understanding Genomic Function

 

Introducing the Real-Time qPCR Learning Center

The Real-Time qPCR Learning Center is an educational resource covering the key steps of the qPCR assay. From RNA isolation, to qPCR setup and analysis, it includes best practices and recommendations from experts on how to improve your qPCR technique.

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