Mini-Sub® Cell GT Cell

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Overview

Mini-Sub® Cell GT Complete Systems*
Catalog # Casting
Gates
Gel Caster UVTP Tray (cm) Combs PowerPac Basic
Power Supply
7 x 7** 7 x 10 8-Well 15-Well
170-4406      
170-4466        
170-4486    
170-4467      
164-0300    
170-4487              
164-0303            

* Mini-Sub cell systems purchased before 1996 (Mini-Sub DNA cell) require casting tray (catalog #170-4330). This tray is not compatible with the Mini-Sub cell GT system.
** Allows casting gels in the cell using casting gates; 7 x 10 gels can be cast with a gel caster.

Sub-Cell Selection Guide
 
  Mini-Sub Cell GT* Wide Mini-Sub Cell GT** Sub-Cell GT Sub-Cell Model 96 Sub-Cell Model 192
Cell size
(W x L x H)
9.2 x 25.5 x 5.6 cm 17.8 x 25.5 x 6.8 cm 18 x 40.5 x 9.4 cm 29 x 30 x 9 cm 29 x 40 x 9 cm
Gel tray sizes (OD)
(W x L)
7 x 7 cm
7 x 10 cm
15 x 7 cm
15 x 10 cm
15 x 10 cm
15 x 15 cm
15 x 20 cm
15 x 25 cm
25 x 10 cm
25 x 15 cm
25 x 10 cm
25 x 15 cm
25 x 20 cm
25 x 25 cm
ReadyAgarose gels accommodated Yes Yes No No No
Sample throughput 8–30*** 10–60*** 1–120† 24–96*** 24–192†
Base buffer volume ~270 ml ~650 ml ~1 L ~2 L ~3 L
Buffer recirculation No No No Yes Yes
Bromophenol blue migration ~4.5 cm/hr (at 75 V) ~4.5 cm/hr (at 75 V) ~3.0 cm/hr (at 75 V) ~6.2 cm/hr (at 200 V) ~5.2 cm/hr (at 200 V)
* Mini ReadySub-Cell GT is a Mini-Sub cell GT dedicated to running ReadyAgarose precast gels, gel size 7 x 10 cm; sample throughput is 8, 12, or 2 x 8. This cell does not include casting gates, tray, or combs.
** Wide mini ReadySub-Cell GT is a wide Mini-Sub cell GT dedicated to running ReadyAgarose precast gels, gel size 15 x 10 cm; sample throughput is 20, 32, 2 x 32, or 4 x 26. This cell does not include casting gates, tray, or combs.
*** Sample throughput value assumes 1–2 combs per gel.
† Sample throughput value assumes 1–4 combs per gel.
Mini-Sub® Cell Comb Selection Guide
Catalog # # of Wells Height* Thickness Width
of Well
Length
of Teeth
Volume
(in 5 mm deep gel)
Combs for Mini-Sub Cell GT Systems
170-4464 15 Fixed 0.75 mm 3 mm 10 mm 11.25 µl
170-4465** 15 Fixed 1.5 mm 3 mm 10 mm 22.5 µl
170-4332 15 Adjustable 1 mm 2.6 mm 10.2 mm 13 µl
170-4462 8 Fixed 0.75 mm 5.5 mm 11 mm 20 µl
170-4463** 8 Fixed 1.5 mm 5.5 mm 11 mm 40 µl
170-4333 8 Adjustable 1 mm 5.5 mm 10.2 mm 27.5 µl
170-4461 2 preparative Fixed 1.5 mm 20 mm 10 mm 150 µl
  2 reference     4 mm   30 µl
170-4460 1 preparative Fixed 1.5 mm 44 mm 10 mm 330 µl
  2 reference     3 mm   22.5 µl
170-4342 1 preparative Adjustable 3 mm 43.3 mm 10.2 mm 645 µl
  2 reference     3 mm   45 µl

* Fixed-height combs must be used with GT gel trays. Adjustable-height combs require comb holder, catalog #170-4331.
** Combs included in systems.

Agarose Gel Casting Guide

Hand Casting Options for a Variety of Gel Sizes

Two accessories are available for hand casting with the Sub-Cell family of electrophoresis cells: gel casters and casting gates. Gel casters offer the flexibility of accommodating several different-sized gel trays. These are available for the entire line of Sub-Cell systems. Casting gates, available in specific sizes for each cell, offer the convenience of casting directly in the Sub-Cell cells.



Gel Caster Guide
Tray Size (cm) Mini Gel Caster Full-Size Gel Caster Model 96 Gel Caster Model 192 Gel Caster
7 x 7
7 x 10
15 x 7
15 x 10
15 x 15  
15 x 20  
15 x 25  
25 x 10    
25 x 15    
25 x 20      
25 x 25      

Casting Guide
Electrophoresis Cell Tray Size (cm) Casting Gates Gel Caster
Mini-Sub cell GT 7 x 7
7 x 10  
Wide Mini-Sub cell GT 15 x 7
15 x 10  
Sub-Cell GT 15 x 10  
15 x 15
15 x 20  
15 x 25  
Sub-Cell Model 96 25 x 10
25 x 15  
Sub-Cell Model 192 25 x 10  
25 x 15
25 x 20  
25 x 25  

The redesigned Mini-Sub cell GT cell offers updated features that make electrophoresis even easier. The Mini-Sub cell GT cell can resolve up to 30 samples; its short, narrow format allows 7 and 10 cm runs with speed, simplicity, and economy.

Bio-Rad's mini cells resolve EcoRI or HindIII digests of lambda DNA in only 1.5 hours at 60 V. Small DNA fragments can be separated in as little as 15 minutes at 150 V.

All mini cells accommodate ReadyAgarose precast gels to save time and allow highly reproducible separations, and include a buffer tank, a safety lid with cables, and a leveling bubble. System configurations that include additional accessories are also available (see the Mini-Sub Cell GT Complete Systems Selection Guide for more details).

Features:

  • QuickSnap* electrodes are easy to remove, simplifying cleaning
  • Arrow on the side of the base indicates the direction of the run and ensures proper orientation of the gel
  • Color-coded, labeled electrodes and labeled base guarantee correct positioning of the lid on the base
  • Tabs on the base permit easy removal of the lid, reducing buffer spillage, and also prevent incorrect lid positioning
  • Reverse-compatible design allows the cells to be used with components from older models
  • Clear plastic construction for easy sample visualization
  • UV-transparent gel trays with fluorescent ruler**
  • Gel-casting gates to cast your own gels right in the cell, or optional caster for tape-free casting**
  • Combs to fit every need (fixed-height drop-in combs, adjustable-height combs, and preparative combs)**

* Patent pending.
** Purchase separately for ReadySub-Cell GT systems.

Mini ReadySub-Cell GT Cells
The mini ReadySub-Cell GT cell is identical to the Mini-Sub cell GT cell, except it is dedicated to running ReadyAgarose precast gels. These gels lock securely into the mini ReadySub-Cell chamber, offering convenience and reproducibility. This cell does not include casting gates, tray, or combs. Kits are available to upgrade ReadySub-Cell GT cells for handcasting capability.

Cell size (W x L x H) 9.2 x 25.5 x 5.6 cm
Gel tray sizes (OD) (W x L) 7 x 7 cm
7 x 10 cm
ReadyAgarose gels accommodated Yes, 8-, 12-, 2 x 8-well
Sample throughput 8–30
Base buffer volume ~270 ml
Buffer recirculation No
Bromophenol blue migration ~4.5 cm/hr (at 75 V)
Mini-Sub Cell GT System

170-4406
Mini horizontal electrophoresis system, includes 8- and 15-well combs, 7 x 7 cm UV-transparent tray, casting gates

List Price:   $367.00
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Mini-Sub® Cell GT System

170-4466
Mini horizontal electrophoresis system, includes 8- and 15-well combs, 7 x 10 cm UV-transparent tray

List Price:   $362.00
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Mini-Sub Cell GT System

170-4486
Mini horizontal electrophoresis system, includes 8- and 15-well combs, 7 x 7 cm UV-transparent tray, casting gates, mini-gel caster

List Price:   $441.00
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Mini-Sub® Cell GT System

170-4467
Mini horizontal electrophoresis system, includes 8- and 15-well combs, 7 x 10 cm UV-transparent tray, mini-gel caster

List Price:   $441.00
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Mini-Sub Cell GT Cell and PowerPac Basic Power Supply

164-0300
Mini horizontal electrophoresis system, includes 8- and 15-well combs, gel caster, 7 x 10 cm UV-transparent tray, basic power supply (#164-5050)

List Price:   $791.00
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Mini ReadySub-Cell™ GT Cell

170-4487
Horizontal electrophoresis cell, includes lid and electrodes, accommodates 8- and 12-well mini ReadyAgarose™ gels

List Price:   $299.00
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Mini ReadySub-Cell™ GT Cell and PowerPac™ Basic Power Supply

164-0303
Horizontal electrophoresis cell system, accommodates 8- and 12-well mini ReadyAgarose™ gels, includes basic power supply (#164-5050)

List Price:   $649.00
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Number Description Options
10016027 Instruction Manual, Sub-Cell GT Agarose Gel Electrophoresis Systems, Rev C Click to download
5878 Sub-Cell Systems Flier, Rev B Click to download [ Add to Cart (Free) ]
2660 Sub-Cell Systems Brochure, Rev D Click to download [ Add to Cart (Free) ]