Overview
Safety Data Sheet
Description
Description
Use our molecular ruler to accurately assign molecular weights to DNA fragments separated by gel electrophoresis. Perfect 50% GC content eliminates spurious migration due to sequence variation, ensuring that the fragments migrate exactly according to their specified size. See Recommended Gel Types. Store product at 4°C.
| Molecular Ruler |
Concentration (µg/µl DNA) |
Size Range | Number of Bands |
Reference Band |
Amount of DNA (µg) |
Suggested Loading Volume (µl) |
Number of Applications |
| AmpliSize (1708200) |
0.1 (10 ng/band/µl) |
50–2,000 bp | 10 of proprietary blunt-ended DNA |
None | 25 | 5 | 50 |
| 20 bp (1708201) |
0.2 | 20–1,000 bp | 50 in 20 bp increments |
200 bp | 50 | 2.5 | 100 |
| EZ Load 20 bp (1708351) |
0.1 | 20–1,000 bp | 50 in 20 bp increments |
200 bp | 50 | 5 | 100 |
| 100 bp* (1708202) |
0.1 | 100–1,000 bp | 10 in 100 bp increments |
None | 25 | 2.5 | 100 |
| EZ Load 100 bp* (1708352) |
0.05 | 100–1,000 bp | 10 in 100 bp increments |
None | 25 | 5 | 100 |
| 100 bp PCR* (1708206) |
0.2 | 100–3,000 bp | 30 in 100 bp increments |
1,000 bp & 3,000 bp |
40 | 2 | 100 |
| EZ Load 100 bp PCR* (1708353) |
0.08 | 100–3,000 bp | 30 in 100 bp increments |
1,000 bp & 3,000 bp |
40 | 5 | 100 |
| 500 bp** (1708203) |
0.2 | 500–8,000 bp | 16 in 500 bp increments |
5,000 bp | 40 | 2 | 100 |
| EZ Load 500 bp** (1708354) |
0.08 | 500–8,000 bp | 16 in 500 bp increments |
5,000 bp | 40 | 5 | 100 |
| 1 kb** (1708204) |
0.2 | 1–15 kb | 15 in 1 kb increments |
5 kb | 40 | 2 | 100 |
| EZ Load 1 kb** (1708355) |
0.08 | 1–15 kb | 15 in 1 kb increments |
5 kb | 40 | 5 | 100 |
| 2.5 kb** (1708205) |
0.1 | 2.5–35 kb | 14 in 2.5 kb increments |
10 kb | 40 | 4 | 100 |
EZ Load Molecular Rulers are preblended with sample loading buffer.
The 100 bp rulers may show a double- or triple-banding pattern in polyacrylamide gels.
*The DNA fragments in this product have HindIII-compatible cohesive ends.
**The DNA fragments in this product have EcoRI-compatible cohesive ends.
For optimal resolution of <500 bp fragments, use a 3% ReadyAgarose Precast Gel or a 5% Mini-PROTEAN TBE Precast Gel. For fragments of >500 bp, use a 1% ReadyAgarose Precast Gel.
Related Products
- Buffers and Reagents for Nucleic Acid Electrophoresis
- Nucleic Acid Stains and Tracking Dyes
- All Nucleic Acid Standards
More Information
Download the Little Book of Standards for complete product information on all Bio-Rad nucleic acid and protein standards.