Certified Genetic Quality Tested (GQT) DNA Grade Agarose

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Overview

Bio-Rad offers seven different Certified Agarose products that are all guaranteed to be free of inhibitors, DNases, and RNases.
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Description

Description

All Bio-Rad Certified Agarose products are guaranteed to be free of inhibitors, DNases, and RNases.

Certified Molecular Biology Agarose — a linear polymer with a very high molecular weight, which results in a gel structure unlike those of traditional agaroses. This general-purpose agarose ensures that DNA recovered from a preparative gel can be manipulated without compromising quality. With a very low sulfate content, this general-purpose agarose produces a gel with a very high gel strength and higher exclusion limit. The high electrophoretic mobility increases resolution and reduces run time, and the gels are easy to handle even at low agarose percentages. Analytical separation is ≥1,000 bp.

Certified PCR Agarose — recommended for separation of DNA fragments ≤1,000 bp. This high-strength agarose forms gels that are easy to handle and that remain flexible even at high gel percentages, which minimizes the risk of cracking or breaking. PCR agarose, unlike GQT products with similar sieving properties that gel at higher temperatures, gels at 40°C so it is faster and easier to prepare.

Certified Low-Range Ultra Agarose — provides superior resolution of small PCR fragments and primers at lower concentrations than standard agaroses. A 3% gel clearly resolves a 10 bp ladder, and a 4% gel approaches the resolution of an 8% polyacrylamide gel. Analytical separation is 20–800 bp.

Certified Megabase Agarose — the superior choice for CHEF and FIGE applications, with a very high gel strength, high electrophoretic mobility, and high exclusion limit. The gels remain easy to handle at concentrations as low as 0.3% and allow shorter run times. The separation range is between 1 kb and 5 Mb. Low background staining also provides superior imaging of high molecular weight DNA.

Certified Low-Melt Agarose — low-melting-temperature agarose with a high resolving capacity for DNA fragments ≥1,000 bp. It is recommended for preparative electrophoresis and for in-gel applications such as digestion, ligation, PCR, transformation, and sequencing. It is also recommended for embedding chromosomes and megabase-sized DNA for pulsed field applications.

Certified PCR Low-Melt Agarose — has a high sieving capacity and yields excellent resolution of fragments ≤1,000 bp in a low-melt or preparative format. This agarose is ideal for digestion by agarase and for all in-gel applications.

Pulsed-Field Certified Agarose — enables excellent separation and resolution of large DNA fragments in pulsed field gel applications. The optimal separation range is 1 kb–2 Mb. Although gels cast with this agarose have longer PFGE run times than Certified Megabase Agarose, running conditions for this agarose are a preset selectable method in the CHEF Mapper XA System.

Ordering

Ordering

items

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1613100
25 g, general purpose agarose for nucleic acid electrophoresis, high gel strength, certified free of inhibitors, DNases, RNases
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1613101
125 g, general purpose agarose for nucleic acid electrophoresis, high gel strength, certified free of inhibitors, DNases, RNases
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1613102
500 g, general purpose agarose for nucleic acid electrophoresis, high gel strength, certified free of inhibitors, DNases, RNases
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1613103
25 g, agarose for separation of DNA fragments ≤1,000 bp, high gel strength, certified free of inhibitors, DNases, RNases
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1613105
500 g, agarose for separation of DNA fragments ≤1,000 bp, high gel strength, certified free of inhibitors, DNases, RNases
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1613106
25 g, agarose for superior resolution of small PCR fragments and primers at low concentrations, certified free of inhibitors, DNases, RNases
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1613107
125 g, agarose for superior resolution of small PCR fragments and primers at low concentrations, certified free of inhibitors, DNases, RNases
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1613108
25 g, agarose for use with CHEF and FIGE applications, certified free of inhibitors, DNases, RNases
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1613109
125 g, agarose for use with CHEF and FIGE applications, certified free of inhibitors, DNases, RNases
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1613110
500 g, agarose for use with CHEF and FIGE applications, certified free of inhibitors, DNases, RNases
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1613111
25 g, recommended for DNA fragments ≥1,000 kb, high sieving capacity low-melting temperature agarose, certified free of inhibitors, DNases, RNases
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1613112
125 g, recommended for DNA fragments ≥1,000 kb, high sieving capacity low-melting temperature agarose, certified free of inhibitors, DNases, RNases
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1613113
25 g, recommended for fragments ≤1,000 bp, high sieving capacity low-melting temperature agarose, certified free of inhibitors, DNases, RNases
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1613114
125 g, recommended for fragments ≤1,000 bp, high sieving capacity low-melting temperature agarose, certified free of inhibitors, DNases, RNases
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1613115
500 g, recommended for fragments ≤1,000 bp, high sieving capacity low-melting temperature agarose, certified free of inhibitors, DNases, RNases
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1620137
100 g, agarose for separation of large DNA in pulsed field gel applications, certified free of inhibitors, DNases, RNases
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1620138
500 g, agarose for separation of large DNA in pulsed field gel applications, certified free of inhibitors, DNases, RNases

Kits & Reagents

Kits & Reagents

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1610433
10 mL ethidium bromide solution for fluorescent staining of nucleic acids, 10 mg/mL
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(Discontinued) EDTA
1610729
500 g, ≥99% pure EDTA (disodium ethylenediaminetetraacetate dihydrate) powder
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(Discontinued) Tris
1610716
500 g, ≥99.8% pure Tris base (Tris[hydroxymethyl]aminomethane or Trimethamine)
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(Discontinued) Tris
1610719
1 kg, ≥99.8% pure Tris base (Tris[hydroxymethyl]aminomethane or Trimethamine)

Documents

Documents

Number Description Options
5552
DNA Analysis Workflow Brochure, Rev B
2755
Certified Agarose Powder Brochure, Rev A