Molecular Rulers

Molecular Rulers

Molecular rulers are DNA standards with precisely defined size intervals between bands (DNA ladders). Bio-Rad provides three types of molecular rulers: standard, EZ Load, and AmpliSize rulers.

Read More
Ordering Information

Overview

Bio-Rad molecular rulers are DNA standards with precisely defined size intervals between bands (DNA ladders) for simplified estimation of the length of nucleic acids separated on agarose gels. Bio-Rad provides three types of molecular rulers: standard, EZ Load, and AmpliSize rulers. To ensure a precise measurement of the size of your samples, most of these DNA ladders include a visually distinct reference band.

Standard Molecular Rulers

Standard molecular rulers consist of precisely sized DNA and are provided ready for dilution. Each vial contains DNA for 100 applications. The advantages of standard molecular rulers include:

  • Visually distinct reference bands
  • Five size ranges to select from
  • No contaminating plasmid DNA

EZ Load Molecular Rulers

Ready-to-load EZ Load molecular rulers consist of precisely sized DNA blended with loading buffer for added convenience (prediluted to a concentration appropriate for most electrophoresis runs). Each vial contains DNA for 100 applications and includes an additional vial of 5x sample buffer for preparing your own samples. The advantages of EZ Load molecular rulers include:

  • No preparation — ready for direct loading on agarose or polyacrylamide gels
  • Visually distinct reference bands
  • Five size ranges to select from
  • No contaminating plasmid DNA

AmpliSize Molecular Ruler

The AmpliSize molecular ruler contains blunt-ended DNA of known sequence at 50% GC content. It has ten bands (at 50, 100, 200, 300, 400, 500, 700, 1,000, 1,500, and 2,000 bp). Each vial contains DNA for 50 applications.

Related Technologies

Related Products

Specifications

20 bp Molecular Ruler
 
Contents
1 vial 20 bp molecular ruler, 250 µl supplied in TE (10 mM Tris-HCl, 1 mM EDTA, pH 8)
Number of applications
100 when used at 2.5 µl per load
Concentration
0.2 µg/µl
Amount of DNA
50 µg DNA
Number of bands
50 in 20 bp increments
Reference bands
200 bp contains 3x the concentration of material found in the other bands
EZ Load™ 20 bp Molecular Ruler
 
Contents 
1 vial EZ Load 20 bp molecular ruler, 500 µl supplied in 5% glycerol, 15 mM Tris pH 8.0, 1.5 mM EDTA, 0.04% bromophenol blue, 0.04% xylene cyanole FF
Number of applications 
100 when used at 5 µl per load
Concentration 
0.1 µg/µl
Amount of DNA 
50 µg DNA  
Number of bands 
50 in 20 bp increments  
Reference bands 
200 bp contains 3x the concentration of material found in the other bands 
100 bp Molecular Ruler
 
Contents  
1 vial 100 bp molecular ruler, 250 µl supplied in TE (10 mM Tris-HCl, 1 mM EDTA, pH 8)
Number of applications  
100 when used at 2.5 µl per load  
Concentration  
0.1 µg/µl  
Amount of DNA  
25 µg DNA  
Number of bands  
10 in 100 bp increments  
Reference bands  
None
EZ Load™ 100 bp Molecular Ruler
 
Contents   
1 vial EZ Load 100 bp molecular ruler, 500 µl supplied in 5% glycerol, 15 mM Tris pH 8.0, 1.5 mM EDTA, 0.04% bromophenol blue, 0.04% xylene cyanole FF
Number of applications
100 when used at 5 µl per load   
Concentration
0.05 µg/µl   
Amount of DNA   
25 µg DNA    
Number of bands   
10 in 100 bp increments    
Reference bands   
None   
100 bp PCR Molecular Ruler            
            
Contents            
1 vial PCR molecular ruler, 200 µl supplied in TE (10 mM Tris-HCl, 1 mM EDTA, pH 8)            
Number of applications            
100 when used at 2 µl per load            
Concentration            
0.2 µg/µl            
Amount of DNA            
40 µg DNA            
Number of bands            
30 in 100 bp increments            
Reference bands            
1,000 and 3,000 bp            
EZ Load 100 bp PCR Molecular Ruler
 
Contents    
1 vial EZ Load 100 bp PCR molecular ruler, 500 µl supplied in 5% glycerol, 15 mM Tris pH 8.0, 1.5 mM EDTA, 0.04% bromophenol blue, 0.04% xylene cyanole FF    
Number of applications    
100 when used at 5 µl per load    
Concentration    
0.08 µg/µl    
Amount of DNA    
40 µg DNA    
Number of bands    
30 in 100 bp increments     
Reference bands    
1,000 bp and 3,000 bp    
500 bp Molecular Ruler    
 
Contents     
1 vial 500 bp molecular ruler, 200 µl supplied in TE (10 mM Tris-HCl, 1 mM EDTA, pH 8)     
Number of applications     
100 when used at 2 µl per load     
Concentration     
0.2 µg/µl     
Amount of DNA     
40 µg DNA      
Number of bands     
16 in 500 bp increments      
Reference band     
5,000 bp     
EZ Load 500 bp Molecular Ruler      
      
Contents      
1 vial EZ Load 500 bp molecular ruler, 500 µl supplied in 5% glycerol, 15 mM Tris pH 8.0, 1.5 mM EDTA, 0.04% bromophenol blue, 0.04% xylene cyanole FF      
Number of applications      
100 when used at 5 µl per load      
Concentration      
0.08 µg/µl      
Amount of DNA      
40 µg DNA       
Number of bands      
16 in 500 bp increments       
Reference bands      
5,000 bp      
1 kb Molecular Ruler       
       
Contents       
1 vial of 1 kb molecular ruler, 200 µl supplied in TE (10 mM Tris-HCl, 1 mM EDTA, pH 8)       
Number of applications       
100 when used at 2 µl per load       
Concentration       
0.2 µg/µl       
Amount of DNA       
40 µg DNA        
Number of bands       
15 in 1 kb increments        
Reference bands       
5 kb       
EZ Load 1 kb Molecular Ruler        
        
Contents          
1 vial EZ Load 1 kb molecular ruler, 500 µl supplied in 5% glycerol, 15 mM Tris pH 8.0, 1.5 mM EDTA, 0.04% bromophenol blue, 0.04% xylene cyanole FF          
Number of applications          
100 when used at 5 µl per load          
Concentration          
0.08 µg/µl          
Amount of DNA          
40 µg DNA           
Number of bands          
15 in 1 kb increments           
Reference bank          
5 kb          
AmpliSize® Molecular Ruler           
           
Contents           
10 fragments of proprietary blunt-ended DNA of precise length and known sequence           
Number of applications           
50 when used at 5 μl per load           
Concentration           
0.1 µg/µl (10 ng/band/µl)           
Amount of DNA           
25 µg DNA            
Reference bands           
None           
2.5 kb Molecular Ruler            
            
Contents            
1 vial 2.5 kb molecular ruler, 400 µl supplied in TE (10 mM Tris-HCl, 1 mM EDTA, pH 8)            
Number of applications            
100 when used at 4 µl per load            
Concentration            
0.1 µg/µl            
Amount of DNA            
40 µg DNA             
Number of bands            
14 in 2.5 kb increments            
Reference band            
10 kb            

Ordering

items

Use the filters below to refine results!

170-8351_tn.jpg
(Discontinued) EZ Load 20 bp Molecular Ruler
1708351
500 μl, 0.1 μg/μl, ready-to-load DNA standard, 20–1,000 bp, 50 bands, includes 1 ml 5x nucleic acid sample buffer, 100 applications
170-8352_tn.jpg
(Discontinued) EZ Load 100 bp Molecular Ruler
1708352
500 μl, 0.05 μg/μl, ready-to-load DNA standard, 100–1,000 bp, 10 bands, includes 1 ml 5x nucleic acid sample buffer, 100 applications
170-8353_tn.jpg
(Discontinued) EZ Load 100 bp PCR Molecular Ruler
1708353
500 μl, 0.08 μg/μl, ready-to-load DNA standard, 100–3,000 bp, 30 bands, includes 1 ml 5x nucleic acid sample buffer, 100 applications
170-8354_tn.jpg
(Discontinued) EZ Load 500 bp Molecular Ruler
1708354
500 μl, 0.08 μg/μl, ready-to-load DNA standard, 500–8,000 bp, 16 bands, includes 1 ml 5x nucleic acid sample buffer, 100 applications
170-8355_tn.jpg
(Discontinued) EZ Load 1 kb Molecular Ruler
1708355
500 μl, 0.08 μg/μl, ready-to-load DNA standard, 1–15 kb, 15 bands, includes 1 ml 5x nucleic acid sample buffer, 100 applications

Accessories

211-2001_tn.jpg
(Discontinued) Xcluda Aerosol Barrier Pipet Tips (Style A), 0.5–10 µl
2112001
Pkg of 960, 0.5–10 µl pipet tip with aerosol barrier, racked, certified free of pyrogen, DNase, RNase
211-2006_tn.jpg
(Discontinued) Xcluda Aerosol Barrier Pipet Tips (Style B), 2–20 µl
2112006
Pkg of 960, 2–20 µl pipet tip with aerosol barrier, racked, certified free of pyrogen, DNase, RNase
211-2011_tn.jpg
(Discontinued) Xcluda Aerosol Barrier Pipet Tips (Style C), 10–100 µl
2112011
Pkg of 1,000, 10–100 µl pipet tip with aerosol barrier, bulk, certified free of pyrogen, DNase, RNase
211-2016_tn.jpg
(Discontinued) Xcluda Aerosol Barrier Pipet Tips (Style D), 20–200 µl
2112016
Pkg of 960, 20–200 µl pipet tip with aerosol barrier, racked, certified free of pyrogen, DNase, RNase
211-2021_tn.jpg
(Discontinued) Xcluda Aerosol Barrier Pipet Tips (Style E), 100–1,000 µl
2112021
Pkg of 960, 100–1,000 µl pipet tip with aerosol barrier, racked, certified free of pyrogen, DNase, RNase
211-2026_tn.jpg
(Discontinued) Xcluda Aerosol Barrier Pipet Tips (Style F), 0.5–10 µl
2112026
Pkg of 1,000, 0.5–10 µl pipet tip with aerosol barrier, bulk, certified free of pyrogen, DNase, RNase
211-2031_tn.jpg
(Discontinued) Xcluda Aerosol Barrier Pipet Tips (Style G), 10–100 µl
2112031
Pkg of 1,000, 10–100 µl pipet tip with aerosol barrier, bulk, certified free of pyrogen, DNase, RNase
211-2036_tn.jpg
(Discontinued) Xcluda Aerosol Barrier Pipet Tips (Style H), 100–1,000 µl
2112036
Pkg of 1,000, 100–1,000 µl pipet tip with aerosol barrier, bulk, certified free of pyrogen, DNase, RNase
223-9911_tn.jpg
(Discontinued) Seque/Pro Capillary Pipet Tips, 0.5–10 µl
2239911

Pkg of 200, 0.5–10 µl capillary pipet tip for gel loading, in enclosed autoclavable rack. Note: This item is now available under the new material number 12021145. For future orders please proceed here.

223-9912_tn.jpg
(Discontinued) Seque/Pro Capillary Pipet Tips, 0.5–10 µl, sterile
2239912
Pkg of 200, 0.5–10 µl capillary pipet tip for gel loading, sterilized in enclosed rack
223-9915_tn.jpg
(Discontinued) Prot/Elec Pipet Tips, 1–200 µl
2239915

Pkg of 1,000, 1–200 µl pipet tip for gel loading, bulk, plastic bag in dust-free box. Note: This item is now available under the new material number 12021138. For future orders please proceed here.

223-9917_tn.jpg
(Discontinued) Prot/Elec Pipet Tips, 1–200 µl
2239917
Pkg of 5 racks, 200 tips per rack (1,000 tips), 1–200 µl pipet tip for gel loading
223-9916_tn.jpg
(Discontinued) Prot/Elec Pipet Tips, 1–200 µl
2239916

Pkg of 10 racks, 96 tips per rack in 8 x 12 format (960 tips), 1–200 µl pipet tip for gel loading. Note: This item is now available under the new material number 12021139. For future orders please proceed here.

223-9480_tn.jpg
(Discontinued) EZ Micro Test Tubes
2239480
Pkg of 500, graduated micro test tube with pierceable top, 1.5 ml, autoclavable, natural
223-9430_tn.jpg
(Discontinued) EZ Micro Test Tubes
2239430

Pkg of 500, graduated micro test tube with pierceable top, 2 ml, autoclavable, natural. Note: This item is now available under the new material number 12021148. For future orders please proceed here.

223-9501_tn.jpg
(Discontinued) Micro Test Tubes
2239501
Pkg of 500, standard micro test tube, capped, 1.5 ml, autoclavable, natural

Documents

Number Description Options
4100090
Instruction Manual, 2.5 kb Molecular Ruler
4100089
Instruction Manual, 1 kb Molecular Ruler
4100088
Instruction Manual, 500 bp Molecular Ruler
4100087
Instruction Manual, 100 bp PCR Molecular Ruler
4100086
Instruction Manual, 100 bp Molecular Ruler
LIT530
Instruction Manual, AmpliSize Molecular Ruler, Rev D
4100085
Instruction Manual, 20 bp Molecular Ruler, Rev C
4006137
Instruction Manual, EZ Load Molecular Rulers
2121
User Guide to Nucleic Acid Standards Bulletin, Rev D
2414
The Little Book of Standards, Rev D