Freeze 'N Squeeze™ DNA Gel Extraction Spin Columns

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Overview

Freeze 'N Squeeze DNA gel extraction spin columns provide a quick and effective means to purify double-stranded DNA fragments from TAE- or TBE-buffered agarose gels.
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Description

Description

Freeze 'N Squeeze DNA gel extraction spin columns provide a quick and effective means to purify double-stranded DNA fragments from TAE- or TBE-buffered agarose gels. Unlike other purification methods which are often time-consuming or utilize toxic chaotropic materials, the Freeze 'N Squeeze spin column method purifies via filtration, using short centrifugation to draw 50–23,000 bp of DNA out of agarose gel slices that have been quickly frozen and thawed.

Key Features

  • Requires less than 1 minute of hands-on time and less than 10 minutes for DNA cleanup
  • No solutions to prepare
  • DNA immediately available for subsequent PCR, subcloning, ligations, and sequencing reactions

Specifications

Specifications

Applications
Recovery of DNA fragments purified from TAE- or TBE-buffered agarose gels
Starting material
≤700 mg gel slice
DNA size
50–23,000 bp
Method
Spin-column extraction of frozen-thawed gel slices
Recovery
4–23 kb: >80% recovery
Time required
<10 min

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7326165
Pkg of 25, columns for agarose gel DNA extraction, 50 bp–23 kb recovery
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7326166
Pkg of 100, columns for agarose gel DNA extraction, 50 bp–23 kb recovery

Documents

Documents

Number Description Options
4106139
Instruction Manual, Freeze N Squeeze DNA Gel Extraction Spin Columns