Freeze 'N Squeeze DNA gel extraction spin columns provide a quick and effective means to purify double-stranded DNA fragments from TAE- or TBE-buffered agarose gels. Unlike other purification methods which are often time-consuming or utilize toxic chaotropic materials, the Freeze 'N Squeeze spin column method purifies via filtration, using short centrifugation to draw 50–23,000 bp of DNA out of agarose gel slices that have been quickly frozen and thawed.
- Requires less than 1 minute of hands-on time and less than 10 minutes for DNA cleanup
- No solutions to prepare
- DNA immediately available for subsequent PCR, subcloning, ligations, and sequencing reactions
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