Criterion™ Tris-HCl Precast Gels

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Overview

Criterion Tris-HCI Gels
Criterion TGX Interactive Gel Selector


Criterion Tris-HCL Precast Gels are ideal for vertical midi gel electrophoresis. They provide Laemmli separation patterns using a standard Tris-glycine running buffer system. Criterion gels are compatible with the Criterion cell (1–2 gels) and the Criterion™ Dodeca™ cell (1–12 gels).

Features and Benefits

  • Reproducible, high-resolution results
  • Fast setup, loading, and run times
  • Numbered, marked well outlines on the cassette ease sample loading and identification
  • Made without SDS, they can also be used with nondenaturing sample and running buffers (native PAGE conditions) to separate proteins by mass-to-charge ratio
  • Use same buffers and band patterns as Laemmli systems
  • Robust system allows separation of complex samples
  • Run 2-D gels in less than a day

Applications and Uses

  • Polyacrylamide gel electrophoresis (PAGE or SDS-PAGE)
  • 2-D gel electrophoresis
  • Accurate molecular weight estimation

Use the Protein Migration Chart to select a gel percentage that will provide optimal resolution of your sample.

Gel dimensions (W x L x thickness), cm 13.3 x 8.7 x 0.1
Cassette dimensions (W x L x thickness), cm 15.0 x 10.6 x .53
Cassette material Styrene copolymer
  Comb material Polycarbonate
Storage tray material PET
Gel storage conditions Store flat at 2–8°C; do not freeze
  Gel shelf life at recommended temperature* 12 weeks
  Buffer volume, ml Upper, 60; lower, 400
Recommended sample buffer (Laemmli, dilute 1:1 with sample) 62.5 mM Tris-HCl, pH 6.8, 2% SDS, 25% glycerol, 0.01% bromophenol blue
Recommended running buffer (Tris/Glycine/SDS) 25 mM Tris, 192 mM glycine, 0.1% SDS, pH 8.3
  Gel chemistry Tris-HCl
  Gel format Midi
* From date of manufacture.  
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10% Criterion™ Tris-HCl Protein Gel, 12+2 well, 45 µl
10% Criterion™ Tris-HCl Protein Gel, 12+2 well, 45 µl

3450009
Pkg of 1, 10% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells

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10% Criterion™ Tris-HCl Protein Gel, 18 well, 30 µl
10% Criterion™ Tris-HCl Protein Gel, 18 well, 30 µl

3450010
Pkg of 1, 10% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells

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10% Criterion™ Tris-HCl Protein Gel, 26 well, 15 µl
10% Criterion™ Tris-HCl Protein Gel, 26 well, 15 µl

3450011
Pkg of 1, 10% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells

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Criterion Tris-HCl Gel #345-9949
10.5–14% Criterion™ Tris-HCl Gel, 12+2 well, 45 µl

3459949
Pkg of 1, 10.5–14% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

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10–20% Criterion™ Tris-HCl Protein Gel, 12+2 well, 45 µl
10–20% Criterion™ Tris-HCl Protein Gel, 12+2 well, 45 µl

3450042
Pkg of 1, 10–20% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells

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10–20% Criterion™ Tris-HCl Protein Gel, 18 well, 30 µl
10–20% Criterion™ Tris-HCl Protein Gel, 18 well, 30 µl

3450043
Pkg of 1, 10–20% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells

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10–20% Criterion™ Tris-HCl Protein Gel, 26 well, 15 µl
10–20% Criterion™ Tris-HCl Protein Gel, 26 well, 15 µl

3450044
Pkg of 1, 10–20% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells

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12.5% Criterion™ Tris-HCl Protein Gel, 18 well, 30 µl
12.5% Criterion™ Tris-HCl Protein Gel, 18 well, 30 µl

3450015
Pkg of 1, 12.5% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells

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12.5% Criterion™ Tris-HCl Protein Gel, 12+2 well, 45 µl
12.5% Criterion™ Tris-HCl Protein Gel, 12+2 well, 45 µl

3450014
Pkg of 1, 12.5% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells

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12.5% Criterion™ Tris-HCl Protein Gel, 26 well, 15 µl
12.5% Criterion™ Tris-HCl Protein Gel, 26 well, 15 µl

3450016
Pkg of 1, 12.5% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells

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12.5% Criterion™ Tris-HCl Protein Gel, Prep+2 well, 700 µl
12.5% Criterion™ Tris-HCl Protein Gel, Prep+2 well, 700 µl

3450017
Pkg of 1, 12.5% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells

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15% Criterion™ Tris-HCl Protein Gel, 12+2 well, 45 µl
15% Criterion™ Tris-HCl Protein Gel, 12+2 well, 45 µl

3450019
Pkg of 1, 15% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells

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15% Criterion™ Tris-HCl Protein Gel, 18 well, 30 µl
15% Criterion™ Tris-HCl Protein Gel, 18 well, 30 µl

3450020
Pkg of 1, 15% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells

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15% Criterion™ Tris-HCl Protein Gel, 26 well, 15 µl
15% Criterion™ Tris-HCl Protein Gel, 26 well, 15 µl

3450021
Pkg of 1, 15% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells

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4–15% Criterion™ Tris-HCl Protein Gel, 12+2 well, 45 µl
4–15% Criterion™ Tris-HCl Protein Gel, 12+2 well, 45 µl

3450027
Pkg of 1, 4–15% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells

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4–15% Criterion™ Tris-HCl Protein Gel, 18 well, 30 µl
4–15% Criterion™ Tris-HCl Protein Gel, 18 well, 30 µl

3450028
Pkg of 1, 4–15% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells

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4–15% Criterion™ Tris-HCl Protein Gel, 26 well, 15 µl
4–15% Criterion™ Tris-HCl Protein Gel, 26 well, 15 µl

3450029
Pkg of 1, 4–15% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells

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$15.00
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4–20% Criterion™ Tris-HCl Protein Gel, 12+2 well, 45 µl
4–20% Criterion™ Tris-HCl Protein Gel, 12+2 well, 45 µl

3450032
Pkg of 1, 4–20% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells

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$15.00
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4–20% Criterion™ Tris-HCl Protein Gel, 18 well, 30 µl
4–20% Criterion™ Tris-HCl Protein Gel, 18 well, 30 µl

3450033
Pkg of 1, 4–20% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells

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4–20% Criterion™ Tris-HCl Protein Gel, 26 well, 15 µl
4–20% Criterion™ Tris-HCl Protein Gel, 26 well, 15 µl

3450034
Pkg of 1, 4–20% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells

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$15.00
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10% Criterion™ Tris-HCl Protein Gel, 11 cm IPG/prep+1 well, 550 µl
10% Criterion™ Tris-HCl Protein Gel, 11 cm IPG/prep+1 well, 550 µl

3450101
Pkg of 1, 10% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells

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$15.00
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12.5% Criterion™ Tris-HCl Protein Gel, 11 cm IPG/prep+1 well, 550 µl
12.5% Criterion™ Tris-HCl Protein Gel, 11 cm IPG/prep+1 well, 550 µl

3450102
Pkg of 1, 12.5% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells

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4–20% Criterion™ Tris-HCl Protein Gel, 11 cm IPG/prep+1 well, 550 µl
4–20% Criterion™ Tris-HCl Protein Gel, 11 cm IPG/prep+1 well, 550 µl

3450104
Pkg of 1, 4–20% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells

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10–20% Criterion™ Tris-HCl Protein Gel, 11 cm IPG/prep+1 well, 550 µl
10–20% Criterion™ Tris-HCl Protein Gel, 11 cm IPG/prep+1 well, 550 µl

3450107
Pkg of 1, 10–20% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells

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5% Criterion™ Tris-HCl Protein Gel, 12+2 well, 45 µl
5% Criterion™ Tris-HCl Protein Gel, 12+2 well, 45 µl

3450001
Discontinued item
No direct replacement

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5% Criterion™ Tris-HCl Protein Gel, 18 well, 30 µl
5% Criterion™ Tris-HCl Protein Gel, 18 well, 30 µl

3450002
Discontinued item
No direct replacement

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5% Criterion™ Tris-HCl Protein Gel, 26 well, 15 µl
5% Criterion™ Tris-HCl Protein Gel, 26 well, 15 µl

3450003
Discontinued item
No direct replacement

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7.5% Criterion™ Tris-HCl Protein Gel, 12+2 well, 45 µl
7.5% Criterion™ Tris-HCl Protein Gel, 12+2 well, 45 µl

3450005
Discontinued item
Replaced with 7.5% Criterion™ TGX™ Precast Midi Precast Midi Protein Gel, 12+2 well, 45 µl ()

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7.5% Criterion™ Tris-HCl Protein Gel, 18 well, 30 µl
7.5% Criterion™ Tris-HCl Protein Gel, 18 well, 30 µl

3450006
Discontinued item
Replaced with 7.5% Criterion™ TGX™ Precast Midi Protein Gel, 18-well, 30 µl (5671024)

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7.5% Criterion™ Tris-HCl Protein Gel, 26 well, 15 µl
7.5% Criterion™ Tris-HCl Protein Gel, 26 well, 15 µl

3450007
Discontinued item
Replaced with 7.5% Criterion™ TGX™ Precast Midi Protein Gel, 26-well, 15 µl (5671025)

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7.5% Criterion™ Tris-HCl Protein Gel, Prep+2 well, 700 µl
7.5% Criterion™ Tris-HCl Protein Gel, Prep+2 well, 700 µl

3450008
Discontinued item
No direct replacement

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10% Criterion™ Tris-HCl Protein Gel, Prep+2 well, 700 µl
10% Criterion™ Tris-HCl Protein Gel, Prep+2 well, 700 µl

3450012
Discontinued item
No direct replacement

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15% Criterion™ Tris-HCl Protein Gel, Prep+2 well, 700 µl
(Discontinued) 15% Criterion™ Tris-HCl Protein Gel, Prep+2 well, 700 µl

3450022
Discontinued item
Replaced with Any kD™ Criterion™ TGX™ Precast Midi Protein Gel, Prep+2 well, 700 µl (5671122)

This product is no longer available. Please contact technical support for more information on replacement product.
18% Criterion™ Tris-HCl Protein Gel, 12+2 well, 45 µl
18% Criterion™ Tris-HCl Protein Gel, 12+2 well, 45 µl

3450023
Discontinued item
Replaced with Any kD™ Criterion™ TGX™ Precast Midi Protein Gel, 12+2 well, 45 µl (5671123)

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$15.00
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18% Criterion™ Tris-HCl Protein Gel, 18 well, 30 µl
18% Criterion™ Tris-HCl Protein Gel, 18 well, 30 µl

3450024
Discontinued item
Replaced with Any kD™ Criterion™ TGX™ Precast Midi Protein Gel, 18-well, 30 µl (5671124)

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$15.00
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18% Criterion™ Tris-HCl Protein Gel, 26 well, 15 µl
18% Criterion™ Tris-HCl Protein Gel, 26 well, 15 µl

3450025
Discontinued item
Replaced with Any kD™ Criterion™ TGX™ Precast Midi Protein Gel, 26-well, 15 µl (5671125)

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$15.00
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18% Criterion™ Tris-HCl Protein Gel, Prep+2 well, 700 µl
18% Criterion™ Tris-HCl Protein Gel, Prep+2 well, 700 µl

3450026
Discontinued item
Replaced with Any kD™ Criterion™ TGX™ Precast Midi Protein Gel, Prep+2 well, 700 µl (5671122)

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$15.00
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4–15% Criterion™ Tris-HCl Protein Gel, Prep+2 well, 700 µl
4–15% Criterion™ Tris-HCl Protein Gel, Prep+2 well, 700 µl

3450030
Discontinued item
Replaced with 4–15% Criterion™ TGX™ Precast Midi Protein Gel, Prep+2 well, 700 µl (5671082)

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$15.00
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4–20% Criterion™ Tris-HCl Protein Gel, Prep+2 well, 700 µl
4–20% Criterion™ Tris-HCl Protein Gel, Prep+2 well, 700 µl

3450035
Discontinued item
Replaced with 4–20% Criterion™ TGX™ Precast Midi Protein Gel, Prep+2 well, 700 µl (5671092)

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$15.00
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8–16% Criterion™ Tris-HCl Protein Gel, 12+2 well, 45 µl
8–16% Criterion™ Tris-HCl Protein Gel, 12+2 well, 45 µl

3450037
Discontinued item
Replaced with 8–16% Criterion™ TGX™ Precast Midi Protein Gel, 12+2 well, 45 µl (5671103)

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8–16% Criterion™ Tris-HCl Protein Gel, 18 well, 30 µl
8–16% Criterion™ Tris-HCl Protein Gel, 18 well, 30 µl

3450038
Discontinued item
Replaced with 8–16% Criterion™ TGX™ Precast Midi Protein Gel, 18-well, 30 µl (5671104)

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8–16% Criterion™ Tris-HCl Protein Gel, 26 well, 15 µl
8–16% Criterion™ Tris-HCl Protein Gel, 26 well, 15 µl

3450039
Discontinued item
Replaced with 8–16% Criterion™ TGX™ Precast Midi Protein Gel, 26-well, 15 µl (5671105)

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$15.00
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8–16% Criterion™ Tris-HCl Protein Gel, Prep+2 well, 700 µl
8–16% Criterion™ Tris-HCl Protein Gel, Prep+2 well, 700 µl

3450040
Discontinued item
Replaced with 8–16% Criterion™ TGX™ Precast Midi Protein Gel, Prep+2 well, 700 µl (5671102)

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$15.00
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10–20% Criterion™ Tris-HCl Protein Gel, Prep+2 well, 700 µl
(Discontinued) 10–20% Criterion™ Tris-HCl Protein Gel, Prep+2 well, 700 µl

3450045
Discontinued item
Replaced with 10–20% Criterion™ TGX™ Precast Midi Protein Gel, Prep+2 well, 700 µl (5671112)

This product is no longer available. Please contact technical support for more information on replacement product.
4–15% Criterion™ Tris-HCl Protein Gel, 11 cm IPG/prep+1 well, 550 µl
4–15% Criterion™ Tris-HCl Protein Gel, 11 cm IPG/prep+1 well, 550 µl

3450103
Discontinued item
Replaced with 4–15% Criterion™ TGX™ Precast Midi Protein Gel, 11 cm IPG/prep+1 well, 550 µl (5671081)

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8–16% Criterion™ Tris-HCl Protein Gel, 11 cm IPG/prep+1 well, 550 µl
8–16% Criterion™ Tris-HCl Protein Gel, 11 cm IPG/prep+1 well, 550 µl

3450105
Discontinued item
Replaced with 8–16% Criterion™ TGX™ Precast Midi Protein Gel, 11 cm IPG/prep+1 well, 550 µl (5671101)

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$15.00
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10.5–14% Criterion™ Tris-HCl Protein Gel, 11 cm IPG/prep+1 well, 550 µl
10.5–14% Criterion™ Tris-HCl Protein Gel, 11 cm IPG/prep+1 well, 550 µl

3450106
Discontinued item
Replaced with Any kD™ Criterion™ TGX™ Gel 11 cm IPG/prep+1 550 µl (5671121)

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$15.00
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10.5–14% Criterion™ Tris-HCl Protein Gel, 18 well, 30 µl
10.5–14% Criterion™ Tris-HCl Protein Gel, 18 well, 30 µl

3459950
Discontinued item
Replaced with Any kD™ Criterion™ TGX™ Precast Midi Protein Gel, 18-well, 30 µl (5671124)

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$15.00
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Criterion Tris-HCl Gel #345-9951
10.5–14% Criterion™ Tris-HCl Gel, 26 well, 15 µl

3459951
Discontinued item
Replaced with Any kD™ Criterion™ TGX™ Precast Midi Protein Gel, 26-well, 15 µl (5671125)

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$15.00
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Number Description Options
0512 Evaluation of Stain-Free Gels for Gel-MS Applications (Poster) Click to download
6040 Electrophoresis Guide, Interactive PDF, Rev B Click to download
2788 Criterion Blotting Tray Insert, Rev A Click to download
2710 Criterion™ Precast Gel System Brochure, Rev C Click to download
5981 Criterion™ Tris-HCl to Criterion™ TGX™ and TGX Stain-Free™ Precast Gels Catalog Number Conversion Chart, Rev B Click to download
6147 Robust Transfer of Proteins to Membranes Across a Wide Range of pI and Mass Using the Trans-Blot Turbo System, Rev B Click to download [ Add to Cart (Free) ]
4110001 Instruction Manual, Criterion™ Gel Application Guide, Ver H Click to download