Criterion™ Tris-HCl Precast Gels

Criterion™ Tris-HCl Precast Gels

Bio-Rad Tris-HCl gels are made without SDS. SDS is added to the sample and running buffer to create standard SDS denaturing electrophoresis conditions.

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Ordering Information

Overview

Criterion Tris-HCL Precast Gels are ideal for vertical midi gel electrophoresis. They provide Laemmli separation patterns using a standard Tris-glycine running buffer system. Criterion gels are compatible with the Criterion cell (1–2 gels) and the Criterion™ Dodeca™ cell (1–12 gels).

Features and Benefits

  • Reproducible, high-resolution results
  • Fast setup, loading, and run times
  • Numbered, marked well outlines on the cassette ease sample loading and identification
  • Made without SDS, they can also be used with nondenaturing sample and running buffers (native PAGE conditions) to separate proteins by mass-to-charge ratio
  • Use same buffers and band patterns as Laemmli systems
  • Robust system allows separation of complex samples
  • Run 2-D gels in less than a day

Applications and Uses

  • Polyacrylamide gel electrophoresis (PAGE or SDS-PAGE)
  • 2-D gel electrophoresis
  • Accurate molecular weight estimation

Use the Protein Migration Chart to select a gel percentage that will provide optimal resolution of your sample.

Specifications

Gel dimensions (W x L x thickness), cm
13.3 x 8.7 x 0.1
Cassette dimensions (W x L x thickness), cm
15.0 x 10.6 x .53
Cassette material
Styrene copolymer
  Comb material
Polycarbonate
Storage tray material
PET
Gel storage conditions
Store flat at 2–8°C; do not freeze
  Gel shelf life at recommended temperature*
12 weeks
  Buffer volume, ml
Upper, 60; lower, 400
Recommended sample buffer (Laemmli, dilute 1:1 with sample)
62.5 mM Tris-HCl, pH 6.8, 2% SDS, 25% glycerol, 0.01% bromophenol blue
Recommended running buffer (Tris/glycine/SDS)
25 mM Tris, 192 mM glycine, 0.1% SDS, pH 8.3
  Gel chemistry
Tris-HCl
* From date of manufacture.
 

Ordering

items

Use the filters below to refine results!

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3450009

Pkg of 1, 10% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells

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3450010

Pkg of 1, 10% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells

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3450011

Pkg of 1, 10% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells

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3459949
Pkg of 1, 10.5–14% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
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3450042

Pkg of 1, 10–20% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells

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3450043

Pkg of 1, 10–20% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells

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3450044

Pkg of 1, 10–20% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells

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3450015

Pkg of 1, 12.5% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells

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3450014

Pkg of 1, 12.5% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells

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3450016

Pkg of 1, 12.5% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells

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3450019

Pkg of 1, 15% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells

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3450020

Pkg of 1, 15% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells

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3450021

Pkg of 1, 15% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells

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3450027

Pkg of 1, 4–15% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells

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3450028

Pkg of 1, 4–15% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells

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3450029

Pkg of 1, 4–15% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells

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3450032

Pkg of 1, 4–20% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells

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3450033

Pkg of 1, 4–20% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells

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3450034

Pkg of 1, 4–20% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells

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3450101

Pkg of 1, 10% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells

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3450102

Pkg of 1, 12.5% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells

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3450104

Pkg of 1, 4–20% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells

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3450107

Pkg of 1, 10–20% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells

Accessories

Kits & Reagents

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(Discontinued) 10x Tris/Glycine/SDS
1610732
Pkg of 1, 1 L, 10x premixed electrophoresis buffer, contains 25 mM Tris, 192 mM glycine, 0.1% SDS, pH 8.3 following dilution to 1x with water
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1610734

Pkg of 1, 1 L, 10x premixed electrophoresis buffer contains 25 mM Tris, 192 mM glycine, pH 8.3 following dilution to 1x with water

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1610744
Pkg of 1, 1 L, 10x premixed electrophoresis buffer, contains 100 mM Tris, 100 mM Tricine, 0.1% SDS, pH 8.3 following dilution to 1x with water
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1610771

Pkg of 1, 5 L cube, 10x premixed electrophoresis buffer contains 25 mM Tris, 192 mM glycine, pH 8.3 following dilution to 1x with water

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(Discontinued) 10x Tris/Glycine/SDS
1610772
Pkg of 1, 5 L cube, 10x premixed electrophoresis buffer, contains 25 mM Tris, 192 mM glycine, 0.1% SDS, pH 8.3 following dilution to 1x with water
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1610738
30 ml, premixed protein sample buffer for native PAGE, contains 62.5 mM Tris-HCl, pH 6.8, 40% glycerol, 0.01% bromophenol blue

Documents

Number Description Options
0512
Evaluation of Stain-Free Gels for Gel-MS Applications (Poster)
6040
Electrophoresis Guide, Interactive PDF, Rev C
2788
Criterion Blotting Tray Insert, Rev A
2710
Criterion™ Precast Gel System Brochure, Rev C
5981
Criterion Tris-HCl to Criterion TGX and TGX Stain-Free Precast Gels Catalog Number Conversion Chart
6147
Robust Transfer of Proteins to Membranes Across a Wide Range of pI and Mass Using the Trans-Blot Turbo System, Rev B
4110001
Instruction Manual, Criterion™ Gel Application Guide, Ver H