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Overview
30 ml, premixed protein sample buffer for native PAGE, contains 62.5 mM Tris-HCl, pH 6.8, 40% glycerol, 0.01% bromophenol blue
Safety Data Sheet
Description
Description
Use Native Sample Buffer to retain native protein structure and mass-to-charge ratios during protein electrophoresis. This sample buffer is nondenaturing, containing no SDS, and has no reducing agent. The mobility of proteins in native gels depends on a number of factors in addition to molecular weight, including protein shape and charge.
- The biological activity of most proteins is retained
- Some protein-protein interactions may be maintained
- Native gel electrophoresis cannot be used for molecular weight estimation
- Since migration is influenced by structure, proteins of the same size can often be easily resolved
- Dry samples can be dissolved directly into the sample buffer
Precast gels that can be used for native electrophoresis
| Precast Gels | Size | Gel Chemistry |
| Mini-PROTEAN® TGX™ | Mini | Laemmli-like, long shelf life, fast separation with high resolution |
| Criterion™ TGX™ | Midi | Laemmli-like, long shelf life, fast separation with high resolution |
| Mini-PROTEAN® TGX Stain-Free™ | Mini | Laemmli-like, long shelf life, fast separation with high resolution, unique trihalo compounds for rapid fluorescent protein detection |
| Criterion™ TGX Stain-Free™ | Midi | Laemmli-like, long shelf life, fast separation with high resolution, unique trihalo compounds for rapid fluorescent protein detection |
| Criterion™ Tris-HCl | Midi | Standard Laemmli |
| Criterion Stain Free™ Tris-HCl | Midi | Standard Laemmli, unique trihalo compounds for rapid fluorescent protein detection |
| Criterion XT Tris-Acetate | Midi | Discontinuous buffer ion fronts form moving boundaries to stack, and then separate proteins |
Note: Most proteins have an acidic or slightly basic pI (~3–8) and are run with the power supply connected to the electrophoresis chamber as for SDS-PAGE. If more basic proteins (pl >8.5) are of interest, change the polarity of the electrodes, since they have a net positive charge.
Related Products
- 10x Tris/Glycine (1610734)
Related Categories
- Running Buffers and Reagents
- Sample Loading Buffers and Reagents
- Electrophoresis Chambers
- Power Supplies
- Protein Stains
- Gel Imaging Systems
Supporting Documentation
- Native Sample Buffer Instruction Manual
- Mini-PROTEAN Precast Gels Instruction Manual and Application Guide
- Criterion Gel Application Guide Instruction Manual