10x Tris/Glycine Buffer for Western Blots and Native Gels #1610771



Pkg of 1, 5 L cube, 10x premixed electrophoresis buffer contains 25 mM Tris, 192 mM glycine, pH 8.3 following dilution to 1x with water

Safety Data Sheet

Safety Data Sheet



Use 10x Tris/Glycine Buffer as a transfer buffer for western blots or as a running buffer for native protein gel electrophoresis.

  • For tank or semi-dry blotting for SDS PAGE gels, usually with the addition of 20% methanol
  • For tank blotting of native gels, without methanol
  • As a running buffer for native gels

Precast gels that can be used for native electrophoresis

Note: Most proteins have an acidic or slightly basic pI (~3–8) and are run with the power supply connected to the electrophoresis chamber as for SDS-PAGE. If more basic proteins (pl >8.5) of interest are being separated, change the polarity of the electrodes, since they have a net positive charge.

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