PROTEAN® II xi Cell

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PROTEAN<sup>&reg;</sup> II xi Cell

Overview

Specifications
  PROTEAN II® xi (16 cm) PROTEAN II xi (20 cm) PROTEAN II XL (20 cm)
Number of gels 1–4 1–4 1–4
Gel size (W x L) 16 x 16 cm (handcast) 16 x 20 cm (handcast) 18.3 x 20 cm (handcast)
18.3 x 19.3 cm (precast)
Glass plate size (W x L)      
              Inner plate 20 x 16 cm 20 x 20 cm 20 x 20 cm
              Outer plate 20 x 18.3 cm 20 x 22.3 cm 20 x 22.3 cm
Spacer length 18.3 cm 22.3 cm 22.3 cm
Typical upper buffer volume 350 ml 350 ml 350 ml
Typical lower buffer volume 1.8 L 1.2 L 1.2 L
Typical run times for SDS-PAGE      
              Without cooling 4 hr 5 hr 5 hr
              With cooling 2.5 hr 3.5 hr 3.5 hr
Recommended power supply PowerPac™ HV or PowerPac Universal PowerPac™ HV or PowerPac Universal PowerPac™ HV or PowerPac Universal
Component Comparison

PROTEAN® II xi and XL (IPG) component comparison

  1. The 4 mm xi and 13 mm XL clamp notch
  2. The 19 mm xi and 8 mm XL spacer
  3. The 181 mm xi and 198 mm XL central cooling core gasket
  4. The 153 mm xi and 184 mm XL comb
Sample Volume per Well for PROTEAN® II xi and XL Combs*
 
Comb Thickness
Number or Type of Wells Well Width 0.5 mm 0.75 mm 1.0 mm 1.5 mm 3.0 mm
25
3.5 mm

60 µl
80 µl
120 µl
20
5.0 mm
54 µl
82 µl
110 µl
164 µl
328 µl
15
6.5 mm
74 µl
110 µl
147 µl
221 µl
442 µl
10
1.0 cm
114 µl
172 µl
229 µl
343 µl
687 µl
5
2.3 cm
522 µl
783 µl
1.57 ml
3
4.0 cm
1.37 ml
Blank
14.5 cm
2.44 ml
3.26 ml
4.88 ml
9.76 ml
2-D Comb
  Reference well
3.5 mm
28 µl
42 µl
84 µl
  Sample well
17.8–17.9 cm
* At standard 25 mm well depth.

The PROTEAN II xi cell is a large-format vertical electrophoresis chamber that accommodates up to four 16 x 16 cm or 16 x 20 cm handcast gels for SDS-PAGE, native PAGE, and agarose gel electrophoresis.
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Ordering Information


  • Description
  • Ordering
  • Accessories
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Description

The PROTEAN® II xi cell is a large format vertical electrophoresis cell that is used for the most common electrophoretic techniques such as SDS-PAGE, native electrophoresis, and agarose gel electrophoresis. The PROTEAN II xi cell is available in two sizes to accommodate up to four* 16 x 16 cm or 16 x 20 cm gels.

Key Features

  • Accommodates handcast gels
  • Patented single-screw clamps on the casting stand that exert uniform pressure along the entire length of the glass plates provide a leakproof seal without agarose plugs or grease
  • Central cooling core can be connected to tap water or a cooled recirculating bath, or filled with coolant, providing smile-free patterns with as little as 1.5 L buffer
  • Choice of glass plates, spacers, and sandwich clamps to cast 2 gel lengths, 16 or 20 cm
  • Ability to cast multiple gradient gels using the PROTEAN II multi-gel casting chamber, the Model 495 gradient former, and the appropriate exponential piston. Use of the gel clip facilitates handling of large gels
  • Notched plates allow up to four 16 x 16 cm or 16 x 20 cm handcast gels to be run simultaneously (not recommended for 2-D applications)
  • Optional frosted glass plates for high-resolution vertical agarose gel electrophoresis of nucleic acids
  • Comb conversion screws convert any PROTEAN II xi comb from a standard 25 mm well depth to a 10 mm depth to simplify comb removal

PROTEAN II IPG Conversion Kits for 2-D Applications

Conversion kits expand the capabilities of the PROTEAN II xi cell to include applications such as tube gel IEF and second-dimension SDS-PAGE. To convert the PROTEAN II xi cell into a:

  • PROTEAN xi 2-D cell — use available adaptors and other accessories to accommodate 16 tube gels for IEF applications
  • PROTEAN II XL cell — use the PROTEAN II IPG conversion kit for second-dimension electrophoresis of 17 cm IPG strips

Each cell includes the central cooling core with gaskets, lower buffer chamber, lid with cables, 2 sets of glass plates, 4 sandwich clamps, upper buffer dam, casting stand with gaskets, leveling bubble, and instructions. The 16 cm cells include clamps for running 16 cm gels; the 20 cm cells include clamps for 20 cm gels. Cells without combs and spacers (catalog #165-1801 and #165-1811) allow you to select the spacer thickness and comb that best fit your application. See the Accessories tab for a list of available combs and spacers.

* For higher throughput, the PROTEAN II xi multi-cell provides 6-gel capacity for second-dimension runs.


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PROTEAN II xi Cell

1651801
Large-format vertical electrophoresis cell, 16 x 16 cm gel size, 4 gel capacity, 2 sets of glass plates, 2 sets of sandwich clamps, casting stand, upper buffer dam, alignment card with leveling bubble; spacers and combs not included

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$1,874.00 
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PROTEAN II xi Cell

1651802
Large format vertical electrophoresis cell, 16 x 16 cm gel size, 4 gel capacity, includes four 1.5 mm spacers, two 15-well combs, 2 sets of glass plates, 2 sets of sandwich clamps, casting stand, upper buffer dam, alignment card with leveling bubble

List Price: 
 
$1,924.00 
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PROTEAN II xi Cell

1651803
Large format vertical electrophoresis cell, 16 x 16 cm gel size, 4 gel capacity, includes four 1.0 mm spacers, two 15-well combs, 2 sets of glass plates, 2 sets of sandwich clamps, casting stand, upper buffer dam, alignment card with leveling bubble

List Price: 
 
$1,987.00 
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PROTEAN II xi Cell

1651804
Large format vertical electrophoresis cell, 16 x 16 cm gel size, 4 gel capacity, includes four 0.75 mm spacers, two 15-well combs, 2 sets of glass plates, 2 sets of sandwich clamps, casting stand, upper buffer dam, alignment card with leveling bubble

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$1,924.00 
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PROTEAN II xi Cell

1651811
Large format vertical electrophoresis cell, 16 x 20 cm gel size, 4 gel capacity, spacers and combs not included

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$1,942.00 
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PROTEAN II xi Cell

1651812
Large format vertical electrophoresis cell, 16 x 20 cm gel size, 4 gel capacity, includes four 1.5 mm spacers, two 15-well combs, 2 sets of glass plates, 2 sets of sandwich clamps, casting stand, upper buffer dam, alignment card with leveling bubble

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$1,936.00 
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PROTEAN II xi Cell

1651813
Large format vertical electrophoresis cell, 16 x 20 cm gel size, 4 gel capacity, includes four 1.0 mm spacers, two 15-well combs, 2 sets of glass plates, 2 sets of sandwich clamps, casting stand, upper buffer dam, alignment card with leveling bubble

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$1,997.00 
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PROTEAN II xi Cell

1651814
Large format vertical electrophoresis cell, 16 x 20 cm gel size, 4 gel capacity, includes four 0.75 mm spacers, two 15-well combs, 2 sets of glass plates, 2 sets of sandwich clamps, casting stand, upper buffer dam, alignment card with leveling bubble

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$1,936.00 
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PROTEAN II xi Cell 2-D Conversion Kit

1651815
Kit converts PROTEAN II xi cell into a tube gel IEF 2-D system; includes 2 tube gel adaptors, 24 glass tubes (1.5 mm ID, 180 mm length), gaskets, grommets, stoppers

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$816.00 
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PROTEAN II xi Basic Unit With Casting Stand

1651834
Vertical electrophoresis system, includes electrophoresis cell with central cooling core, gel casting stand

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$1,649.00 
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Accessories

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If you are placing an order, you may proceed with your order; the account price will be applied if it is lower than the
list price. If the problem persists, please call customer service at or send an
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Inner Glass Plates

1651821
Pkg of 2, 16 x 20 cm, glass plates for 16 cm PROTEAN II xi electrophoresis cell only, use with outer plate (#165-1822)

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$67.00 
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Outer Glass Plates

1651822
Pkg of 2, 18.3 x 20 cm, glass plates for 16 cm PROTEAN II xi electrophoresis cell only, use with inner plate (#165-1821)

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$67.00 
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Frosted Inner Glass Plates

1651825
Pkg of 2, 16 x 20 cm, glass plates for agarose gels, for 16 cm PROTEAN II xi electrophoresis cell only, use with outer plate (#165-1822)

This product is being discontinued soon. Please contact customer service for more information.

The following replacement is recommended:

Beveled Inner Glass Plates

1651827
Pkg of 2, 16 x 20 cm, 16 cm bevel length, for 2-D tube gel procedures, for PROTEAN II xi 2-D electrophoresis cell, use with outer plate (#165-1822)

This product is being discontinued soon. Please contact customer service for more information.

The following replacement is recommended:

Notched Inner Glass Plate

1651832
Pkg of 1, 16 x 20 cm, 16 cm bevel length, glass plate for double-up procedures, for 16 cm PROTEAN II xi electrophoresis cell only

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$141.00 
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PROTEAN II Sandwich Clamps

1651901
Pkg of 2, clamps for running gels, for 16 cm PROTEAN II xi electrophoresis cell, 1 left and 1 right

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$157.00 
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Replacement Electrode Power Cables

1651805
Pkg of 1 set, replacement electrode cables, for PROTEAN II xi electrophoresis cells

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$141.00 
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Central Cooling Core

1651806
Pkg of 1, cooling device for PROTEAN II xi and PROTEAN II XL electrophoresis cells; includes 2 gaskets

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$1,136.00 
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Buffer Tank

1651807
Pkg of 1, buffer tank for PROTEAN II xi and PROTEAN II XL electrophoresis cells

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$652.00 
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Cell Lid

1651808
Pkg of 1, lid for PROTEAN II xi and PROTEAN II XL electrophoresis cells; includes power cables

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$287.00 
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PROTEAN II Comb Conversion Screws

1651859
Pkg of 10 each comb conversion and standard screws, for conversion of 2 PROTEAN II xi combs from 25 mm to 10 mm well depth (agarose gels)

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$37.00 
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Upper Buffer Dam

1651909
Pkg of 1, buffer dam for PROTEAN II xi and PROTEAN II XL electrophoresis cells

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$106.00 
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Slab Gel Casting Stand

1651911
Pkg of 1, casting stand for PROTEAN II xi and PROTEAN II XL electrophoresis cells; includes 2 gaskets

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$264.00 
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Replacement Gaskets

1651912
Pkg of 2, replacement gasket, for PROTEAN II xi and PROTEAN II XL casting stand

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$49.00 
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PROTEAN II xi Replacement Gaskets

1651913
Pkg of 2, gasket, for central cooling core of PROTEAN II xi electrophoresis cell

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$68.00 
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Tube Gel Adaptor

1651940
Pkg of 1, adaptor for PROTEAN II xi 2-D electrophoresis cell; includes gasket, grommets (4–8 mm OD tubes), and stoppers

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$405.00 
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Tube Gel Loading Needles

1651943
Pkg of 2, 18 cm, 22 gauge, blunt tip, luer hub, needle for casting monomer in small-diameter tubes

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$98.00 
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Tube Gel Extrusion Needles

1651944
Pkg of 2, 9 cm, 26 gauge, beveled tip, luer hub, needle for removing gels from tubes

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$98.00 
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Replacement Gaskets

1651947
Pkg of 2, replacement gasket for tube gel adaptor

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$68.00 
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PROTEAN II xi Alignment Cards

1652029
Pkg of 2, alignment cards for PROTEAN II xi electrophoresis cell

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$26.00 
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PROTEAN II xi Spacers

1651841
Pkg of 4, 0.5 mm, spacers for 16 cm PROTEAN II xi electrophoresis cell

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$105.00 
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PROTEAN II xi Spacers

1651842
Pkg of 4, 0.75 mm, spacers for 16 cm PROTEAN II xi electrophoresis cell

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$105.00 
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PROTEAN II xi Spacers

1651843
Pkg of 4, 1.0 mm, spacers for 16 cm PROTEAN II xi electrophoresis cell

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$105.00 
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PROTEAN II xi Spacers

1651844
Pkg of 4, 1.5 mm, spacers for 16 cm PROTEAN II xi electrophoresis cell

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$105.00 
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PROTEAN II xi Spacers

1651845
Pkg of 4, 3.0 mm, spacers for 16 cm PROTEAN II xi electrophoresis cell

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$105.00 
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PROTEAN II xi Spacers

1651846
Pkg of 4, 0.5 mm, spacers for 20 cm PROTEAN II xi electrophoresis cell

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$105.00 
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PROTEAN II xi Spacers

1651847
Pkg of 4, 0.75 mm, spacers for 20 cm PROTEAN II xi electrophoresis cell

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$105.00 
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PROTEAN II xi Spacers

1651848
Pkg of 4, 1.0 mm, spacers for 20 cm PROTEAN II xi electrophoresis cell

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$105.00 
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PROTEAN II xi Spacers

1651849
Pkg of 4, 1.5 mm, spacers for 20 cm PROTEAN II xi electrophoresis cell

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$105.00 
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PROTEAN II xi Spacers

1651850
Pkg of 4, 3.0 mm, spacers for 20 cm PROTEAN II xi electrophoresis cell

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$105.00 
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PROTEAN II xi Comb

1651861
Pkg of 1, 25-well, 0.75 mm, comb for PROTEAN II xi electrophoresis cell

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$73.00 
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PROTEAN II xi Comb

1651862
Pkg of 1, 25-well, 1.0 mm, comb for PROTEAN II xi electrophoresis cell

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$73.00 
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PROTEAN II xi Comb

1651863
Pkg of 1, 25-well, 1.5 mm, comb for PROTEAN II xi electrophoresis cell

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$73.00 
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PROTEAN II xi Comb

1651865
Pkg of 1, 20-well, 0.5 mm, comb for PROTEAN II xi electrophoresis cell

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$73.00 
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PROTEAN II xi Comb

1651866
Pkg of 1, 20-well, 0.75 mm, comb for PROTEAN II xi electrophoresis cell

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$73.00 
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PROTEAN II xi Comb

1651867
Pkg of 1, 20-well, 1.0 mm, comb for PROTEAN II xi electrophoresis cell

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$73.00 
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PROTEAN II xi Comb

1651868
Pkg of 1, 20-well, 1.5 mm, comb for PROTEAN II xi electrophoresis cell

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$73.00 
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PROTEAN II xi Comb

1651869
Pkg of 1, 20-well, 3.0 mm, comb for PROTEAN II xi electrophoresis cell

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$73.00 
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PROTEAN II xi Comb

1651870
Pkg of 1, 15-well, 0.5 mm, comb for PROTEAN II xi electrophoresis cell

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$73.00 
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PROTEAN II xi Comb

1651871
Pkg of 1, 15-well, 0.75 mm, comb for PROTEAN II xi electrophoresis cell

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$73.00 
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PROTEAN II xi Comb

1651872
Pkg of 1, 15-well, 1.0 mm, comb for PROTEAN II xi electrophoresis cell

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$73.00 
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PROTEAN II xi Comb

1651873
Pkg of 1, 15-well, 1.5 mm, comb for PROTEAN II xi electrophoresis cell

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$73.00 
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PROTEAN II xi Comb

1651874
Pkg of 1, 15-well, 3.0 mm, comb for PROTEAN II xi electrophoresis cell

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$73.00 
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PROTEAN II xi Comb

1651875
Pkg of 1, 10-well, 0.5 mm, comb for PROTEAN II xi electrophoresis cell

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$73.00 
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PROTEAN II xi Comb

1651876
Pkg of 1, 10-well, 0.75 mm, comb for PROTEAN II xi electrophoresis cell

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$73.00 
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PROTEAN II xi Comb

1651877
Pkg of 1, 10-well, 1.0 mm, comb for PROTEAN II xi electrophoresis cell

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$73.00 
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PROTEAN II xi Comb

1651878
Pkg of 1, 10-well, 1.5 mm, comb for PROTEAN II xi electrophoresis cell

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$73.00 
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PROTEAN II xi Comb

1651879
Pkg of 1, 10-well, 3.0 mm, comb for PROTEAN II xi electrophoresis cell

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$73.00 
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PROTEAN II xi Comb

1651882
Pkg of 1, 5-well, 1.0 mm, comb for PROTEAN II xi electrophoresis cell

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$73.00 
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PROTEAN II xi Comb

1651883
Pkg of 1, 5-well, 1.5 mm, comb for PROTEAN II xi electrophoresis cell

List Price: 
 
$73.00 
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PROTEAN II xi Comb

1651884
Pkg of 1, 5-well, 3.0 mm, comb for PROTEAN II xi electrophoresis cell

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$73.00 
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PROTEAN II xi Comb

1651888
Pkg of 1, 3-well, 1.5 mm, comb for PROTEAN II xi electrophoresis cell

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$73.00 
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