PROTEAN® i12™ IEF System

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Overview

The PROTEAN i12 system has individual lane control, giving you the unique ability to run different samples, pH gradients, and protocols simultaneously.
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Description

Description

The PROTEAN i12 IEF system is designed for isoelectric focusing (IEF) proteins in immobilized pH gradient (IPG) strips for the first dimension of two-dimensional (2-D) electrophoresis. The PROTEAN i12 IEF cell can run 1–12 IPG strips in 7, 11, 13, 17, 18, or 24 cm focusing trays. Each channel in the isoelectric focusing tray is independently powered, enabling precise control over each IPG strip. This feature makes it possible to run different sample types, different pH gradients, and multiple protocols simultaneously.

Learn more about isoelectric focusing with the PROTEAN i12 IEF system.

Features and Benefits of the PROTEAN i12 IEF System

  • Optimize experiments in fewer runs — the PROTEAN i12 IEF system has the unique ability to run channels under multiple conditions in a single run. Different samples, protocols, and pH gradients can be run simultaneously.
  • Obtain higher-quality data with less experimental risk — each channel is independent, so one irregular sample can no longer compromise the entire run.
  • Easy, intuitive programming — easily run, create, and edit isoelectric focusing protocols from the touch screen user interface. Preprogrammed protocols stored in the internal memory serve as a convenient starting point for developing optimized sample-specific IEF conditions.
  • Run gel side up, gel side down, or with loading cups — focusing trays and electrode assemblies accommodate all possible gel configurations.

Applications and Uses of the PROTEAN i12 IEF System

Isoelectric focusing is the first dimension of 2-D electrophoresis, a powerful technique used for the visualization and identification of proteins. The PROTEAN i12 IEF system can be used for a number of applications including the following:

  • 2-D electrophoresis and analysis
  • Protein profiling — analysis of complex protein mixtures
  • Biomarker discovery — discovery/identification of specific targets and markers
  • Functional proteomics — structure, interactions, and function

More Information

PROTEAN i12 Reporter, a free web-based application found at www.i12reporter.com, can be used to upload run data files, view electronic profiles for individual lanes, and compare sample profiles from different runs. The application can be used to generate reports, print graphs, and create protocols. Protocols created with the application can be transferred to the PROTEAN i12 IEF cell using a USB flash drive.

Bio-Rad offers a complete selection of products to simplify your 2-D electrophoresis workflow and ensure high-quality results. Select from optimized kits and reagents for protein sample preparation, including kits designed for protein sample cleanup to remove contaminants from your samples prior to 2-D electrophoresis. Select the best ReadyStrip™ IPG strip pH gradient and strip length options for your applications. For comprehensive solutions and suggestions for 2-D gel issues, see Troubleshooting 2-D Gels with 2-D Doctor™. Watch the 2-D Tutorial Video to learn how to run great 2-D gels with practical tips from the proteomics experts at Bio-Rad. Bio-Rad’s PDQuest™ 2-D analysis software provides tools for comprehensive and flexible 2-D gel electrophoretic analysis.

Visit the Applications & Technologies pages to learn more about protein electrophoresis and the unique 2-D electrophoresis technologies found in Bio-Rad instruments.

Ordering

Ordering

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1646000
Isoelectric focusing system, includes basic unit, electrode assemblies, focusing and rehydration/equilibration trays, forceps, electrode wicks, mineral oil, cleaning accessories, IPG strips and sample/rehydration buffer, USB flash drives, styluses
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1646001
Isoelectric focusing cell, includes basic unit, electrode assemblies, and 3 styluses. Focusing trays and other accessories sold separately.

Accessories

Accessories

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(Discontinued) Forceps
1654070
Pkg of 1, single pair, fine-tipped forceps for handling immobilized pH gradient (IPG) strips
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(Discontinued) Cleaning Brushes
1654072
Pkg of 2, cleaning brushes, for use with the PROTEAN® i12™ IEF and PROTEAN IEF systems
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(Discontinued) Mineral Oil
1632129
500 ml of mineral oil for use with the PROTEAN® i12™ IEF and PROTEAN IEF systems
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(Discontinued) Cleaning Concentrate
1610722
1 kg, 50x alkaline detergent for cleaning glass plates and other laboratory equipment

Kits & Reagents

Kits & Reagents

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1632105
2-D gel electrophoresis kit, includes protein sample and buffers for IPG strip rehydration, focusing, and transfer to second dimension (IPG strips not included)
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1632106
10 ml, protein sample buffer, includes 8 M urea, 2% CHAPS, 50 mM DTT, 0.2% Bio-Lyte® 3/10 ampholyte, 0.001% Bromophenol Blue
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1632083
10 ml, protein solubilization reagent, includes 7 M urea, 2 M thiourea, 1% ASB-14, 40 mM Tris, 0.001% Bromophenol Blue
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1632094
1 ml, 100x, broad pH range (~3.5–9.5) ampholytes for use in IEF and with ReadyStrip™ IPG strips
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1632093
1 ml, ampholyte concentrate for use in IEF and with ReadyStrip IPG strips
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1632098
1 ml, for use in IEF and with ReadyStrip IPG strips
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1632097
1 ml, for use in IEF and with ReadyStrip IPG strips
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1632096
1 ml, for use in IEF and with ReadyStrip IPG strips
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1632095
1 ml, for use in IEF and with ReadyStrip IPG strips

Trays

Trays

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1654035
Pkg of 25, 7 cm rehydration/equilibration trays, hold up to twelve 7 cm IPG strips, for use with the PROTEAN® i12™ IEF and PROTEAN IEF systems
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1654025
Pkg of 25, 11 cm rehydration/equilibration trays, hold up to twelve 11 cm IPG strips, for use with the PROTEAN® i12™ IEF and PROTEAN IEF systems
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1654015
Pkg of 25, 17 cm rehydration/equilibration trays, hold up to twelve 17 cm IPG strips, for use with the PROTEAN® i12™ IEF and PROTEAN IEF systems
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1654041
Pkg of 25, 18 cm rehydration/equilibration trays, hold up to twelve 18 cm IPG strips, for use with the PROTEAN® i12™ IEF and PROTEAN IEF systems
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1654043
Pkg of 25, 24 cm rehydration/equilibration trays, hold up to twelve 24 cm IPG strips, for use with the PROTEAN® i12™ IEF and PROTEAN IEF systems

IPG Strips

IPG Strips

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1632000
Pkg of 12, 7 cm, pH 3–10, immobilized pH gradient (IPG) strip for first-dimension separations on the PROTEAN® i12™ IEF and PROTEAN IEF systems
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1632001
Pkg of 12, 7 cm, pH 4–7, immobilized pH gradient (IPG) strip for first-dimension separations on the PROTEAN® i12™ IEF and PROTEAN IEF systems
163-2002_tn.jpg
1632002
Pkg of 12, 7 cm, pH 3–10 nonlinear, immobilized pH gradient (IPG) strips for first-dimension separations on the PROTEAN® i12™ IEF and PROTEAN IEF systems
163-2003_tn.jpg
1632003
Pkg of 12, 7 cm, pH 3–6, immobilized pH gradient (IPG) strip for first-dimension separations on the PROTEAN® i12™ IEF and PROTEAN IEF systems
163-2004_tn.jpg
1632004
Pkg of 12, 7 cm, pH 5–8, immobilized pH gradient (IPG) strip for first-dimension separations on the PROTEAN® i12™ IEF and PROTEAN IEF systems
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1632005
Pkg of 12, 7 cm, pH 7–10, immobilized pH gradient (IPG) strip for first-dimension separations on the PROTEAN® i12™ IEF and PROTEAN IEF systems
163-2028_tn.jpg
1632028
Pkg of 12, 7 cm, pH 3.9–5.1, immobilized pH gradient (IPG) strip for first-dimension separations on the PROTEAN® i12™ IEF and PROTEAN IEF systems
163-2029_tn.jpg
1632029
Pkg of 12, 7 cm, pH 4.7–5.9, immobilized pH gradient (IPG) strip for first-dimension separations on the PROTEAN® i12™ IEF and PROTEAN IEF systems
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1632030
Pkg of 12, 7 cm, pH 5.5–6.7, immobilized pH gradient (IPG) strip for first-dimension separations on the PROTEAN® i12™ IEF and PROTEAN IEF systems
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1632031
Pkg of 12, 7 cm, pH 6.3–8.3, immobilized pH gradient (IPG) strip for first-dimension separations on the PROTEAN® i12™ IEF and PROTEAN IEF systems
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1632014
Pkg of 12, 11 cm, pH 3–10, immobilized pH gradient (IPG) strip for first-dimension separations on the PROTEAN® i12™ IEF and PROTEAN IEF systems
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1632015
Pkg of 12, 11 cm, pH 4–7, immobilized pH gradient (IPG) strip for first-dimension separations on the PROTEAN® i12™ IEF and PROTEAN IEF systems
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1632016
Pkg of 12, 11 cm, pH 3–10 nonlinear, immobilized pH gradient (IPG) strip for first-dimension separations on the PROTEAN® i12™ IEF and PROTEAN IEF systems
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1632017
Pkg of 12, 11 cm, pH 3–6, immobilized pH gradient (IPG) strip for first-dimension separations on the PROTEAN® i12™ IEF and PROTEAN IEF systems
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1632018
Pkg of 12, 11 cm, pH 5–8, immobilized pH gradient (IPG) strip for first-dimension separations on the PROTEAN® i12™ IEF and PROTEAN IEF systems
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1632019
Pkg of 12, 11 cm, pH 7–10, immobilized pH gradient (IPG) strip for first-dimension separations on the PROTEAN® i12™ IEF and PROTEAN IEF systems
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1632024
Pkg of 12, 11 cm, pH 3.9–5.1, immobilized pH gradient (IPG) strip for first-dimension separations on the PROTEAN® i12™ IEF and PROTEAN IEF systems
163-2026_tn.jpg
1632026
Pkg of 12, 11 cm, pH 5.5–6.7, immobilized pH gradient (IPG) strip for first-dimension separations on the PROTEAN® i12™ IEF and PROTEAN IEF systems
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1632027
Pkg of 12, 11 cm, pH 6.3–8.3, immobilized pH gradient (IPG) strip for first-dimension separations on the PROTEAN® i12™ IEF and PROTEAN IEF systems
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1632007
Pkg of 12, 17 cm, pH 3–10, immobilized pH gradient (IPG) strip for first-dimension separations on the PROTEAN® i12™ IEF and PROTEAN IEF systems
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1632008
Pkg of 12, 17 cm, pH 4–7, immobilized pH gradient (IPG) strip for first-dimension separations on the PROTEAN® i12™ IEF and PROTEAN IEF systems
163-2009_tn.jpg
1632009
Pkg of 12, 17 cm, pH 3–10 nonlinear, immobilized pH gradient (IPG) strip for first-dimension separations on the PROTEAN® i12™ IEF and PROTEAN IEF systems
163-2010_tn.jpg
1632010
Pkg of 12, 17 cm, pH 3–6, immobilized pH gradient (IPG) strip for first-dimension separations on the PROTEAN® i12™ IEF and PROTEAN IEF systems
163-2011_tn.jpg
1632011
Pkg of 12, 17 cm, pH 5–8, immobilized pH gradient (IPG) strip for first-dimension separations on the PROTEAN® i12™ IEF and PROTEAN IEF systems
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1632012
Pkg of 12, 17 cm, pH 7–10, immobilized pH gradient (IPG) strip for first-dimension separations on the PROTEAN® i12™ IEF and PROTEAN IEF systems
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1632020
Pkg of 12, 17 cm, pH 3.9–5.1, immobilized pH gradient (IPG) strip for first-dimension separations on the PROTEAN® i12™ IEF and PROTEAN IEF systems
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1632021
Pkg of 12, 17 cm, pH 4.7–5.9, immobilized pH gradient (IPG) strip for first-dimension separations on the PROTEAN® i12™ IEF and PROTEAN IEF systems
163-2022_tn.jpg
1632022
Pkg of 12, 17 cm, pH 5.5–6.7, immobilized pH gradient (IPG) strip for first-dimension separations on the PROTEAN® i12™ IEF and PROTEAN IEF systems
163-2023_tn.jpg
1632023
Pkg of 12, 17 cm, pH 6.3–8.3, immobilized pH gradient (IPG) strip for first-dimension separations on the PROTEAN® i12™ IEF and PROTEAN IEF systems
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1632032
Pkg of 12, 18 cm, pH 3–10, immobilized pH gradient (IPG) strip for first-dimension separations on the PROTEAN® i12™ IEF and PROTEAN IEF systems
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1632033
Pkg of 12, 18 cm, pH 3–10 nonlinear, immobilized pH gradient (IPG) strip for first-dimension separations on the PROTEAN® i12™ IEF and PROTEAN IEF systems
163-2034_tn.jpg
1632034
Pkg of 12, 18 cm, pH 4–7, immobilized pH gradient (IPG) strip for first-dimension separations on the PROTEAN® i12™ IEF and PROTEAN IEF systems
163-2035_tn.jpg
1632035
Pkg of 12, 18 cm, pH 3–6, immobilized pH gradient (IPG) strip for first-dimension separations on the PROTEAN® i12™ IEF and PROTEAN IEF systems
163-2036_tn.jpg
1632036
Pkg of 12, 18 cm, pH 5–8, immobilized pH gradient (IPG) strip for first-dimension separations on the PROTEAN® i12™ IEF and PROTEAN IEF systems
163-2037_tn.jpg
1632037
Pkg of 12, 18 cm, pH 7–10, immobilized pH gradient (IPG) strip for first-dimension separations on the PROTEAN® i12™ IEF and PROTEAN IEF systems
163-2038_tn.jpg
1632038
Pkg of 12, 18 cm, pH 3.9–5.1, immobilized pH gradient (IPG) strip for first-dimension separations on the PROTEAN® i12™ IEF and PROTEAN IEF systems
163-2039_tn.jpg
1632039
Pkg of 12, 18 cm, pH 4.7–5.9, immobilized pH gradient (IPG) strip for first-dimension separations on the PROTEAN® i12™ IEF and PROTEAN IEF systems
163-2040_tn.jpg
1632040
Pkg of 12, 18 cm, pH 5.5–6.7, immobilized pH gradient (IPG) strip for first-dimension separations on the PROTEAN® i12™ IEF and PROTEAN IEF systems
163-2041_tn.jpg
1632041
Pkg of 12, 18 cm, pH 6.3–8.3, immobilized pH gradient (IPG) strip for first-dimension separations on the PROTEAN® i12™ IEF and PROTEAN IEF systems
163-2042_tn.jpg
1632042
Pkg of 12, 24 cm, pH 3–10, immobilized pH gradient (IPG) strip with barcode for first-dimension separations on the PROTEAN® i12™ IEF and PROTEAN IEF systems
163-2043_tn.jpg
1632043
Pkg of 12, 24 cm, pH 3–10, nonlinear, immobilized pH gradient (IPG) strip with barcode for first-dimension separations on the PROTEAN® i12™ IEF and PROTEAN IEF systems
163-2044_tn.jpg
1632044
Pkg of 12, 24 cm, pH 4–7, immobilized pH gradient (IPG) strip with barcode for first-dimension separations on the PROTEAN® i12™ IEF and PROTEAN IEF systems
163-2045_tn.jpg
1632045
Pkg of 12, 24 cm, pH 3–6, immobilized pH gradient (IPG) strip with barcode for first-dimension separations on the PROTEAN® i12™ IEF and PROTEAN IEF systems
163-2046_tn.jpg
1632046
Pkg of 12, 24 cm, pH 5–8, immobilized pH gradient (IPG) strip with barcode for first-dimension separations on the PROTEAN® i12™ IEF and PROTEAN IEF systems
163-2047_tn.jpg
1632047
Pkg of 12, 24 cm, pH 7–10, immobilized pH gradient (IPG) strip with barcode for first-dimension separations on the PROTEAN® i12™ IEF and PROTEAN IEF systems
163-2048_tn.jpg
1632048
Pkg of 12, 24 cm, pH 3.9–5.1, immobilized pH gradient (IPG) strip with barcode for first-dimension separations on the PROTEAN® i12™ IEF and PROTEAN IEF systems
163-2049_tn.jpg
1632049
Pkg of 12, 24 cm, pH 4.7–5.9, immobilized pH gradient (IPG) strip with barcode for first-dimension separations on the PROTEAN® i12™ IEF and PROTEAN IEF systems
163-2050_tn.jpg
1632050
Pkg of 12, 24 cm, pH 5.5–6.7, immobilized pH gradient (IPG) strip with barcode for first-dimension separations on the PROTEAN® i12™ IEF and PROTEAN IEF systems
163-2051_tn.jpg
1632051
Pkg of 12, 24 cm, pH 6.3–8.3, immobilized pH gradient (IPG) strip with barcode for first-dimension separations on the PROTEAN® i12™ IEF and PROTEAN IEF systems

Documents

Documents

Number Description Options
2621
PROTEAN Plus Dodeca Cell System Brochure, Rev C
6138
PROTEAN i12 IEF System: Independent Voltage and Current Control Enables Optimization of First-Dimension IEF Conditions, Rev A
6097
PROTEAN i12 IEF System Brochure, Ver B
[ Add to Cart (Free) ]
6139
Versatile Separation Capabilities of the PROTEAN i12 IEF System, Rev A
6140
Use of the PROTEAN® i12 IEF System for In-Gel Peptide Fractionation Prior to LC-MS and Comparison with Off-Gel Fractionation, Rev A
4006166
Instruction Manual, ReadyStrip IPG Strip
10022069
Instruction Manual, PROTEAN i12 IEF System, Rev A
10022548
Instruction Manual, PROTEAN i12 IEF Cell Quick Guide, Rev A
6493
Bio-Rad’s Host Cell Protein (HCP) Workflow Quick Guide
[ Add to Cart (Free) ]