Overview
Immun-Blot® PVDF Membrane is ideal for chemiluminescent and colorimetric western blots: it retains target protein very strongly during protein transfer but also reduces nonspecific protein binding that can obscure high-sensitivity detection.
Features and Benefits
- Mechanical strength of Immun-Blot PVDF membrane enables multiple reprobing of blots
- Naturally hydrophobic support is ideal for western blotting because it ensures high signal with low background
- Available in three formats:
- Convenient precut membrane/blot absorbent filter paper sandwiches
- Packs of precut membrane-only sheets
- Economical rolls
Applications
- Transferring proteins from SDS-PAGE gels to membrane
- Immunoblotting target proteins
- Detection by western blotting chemiluminescent or colorimetric methods
Packaging Options
Immun-Blot PVDF Membrane | Dimensions | Sheets/package | Catalog Number |
---|---|---|---|
Roll | 26 cm x 3.3 m | — | 1620177 |
Precut for mini gels | 7 x 8.4 cm | 10 | 1620174 |
Precut for midi gels | 10 x 15 cm | 10 | 1620175 |
Precut for PROTEAN II XL Gels | 20 x 20 cm | 10 | 1620176 |
Precut for large-format gels | 25 x 28 cm | 10 | 1620255 |
Related Products
- Western Blotting Membranes and Filter Paper
- Western Blotting Transfer Buffers
- Immunodetection Kits and Reagents
Related Categories
Online Resources
- Blotting Membrane and Filter Paper Selection Guide
- Membranes and Blotting Papers
- Protein Blotting Methods
- Western Blotting
Related Technologies
Related Products
Ordering
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Image
(Discontinued) Immun-Blot PVDF Membrane, Roll, 26 cm x 3.3 m
1620177
Pkg of 1 roll, 0.2 µm, 26 cm x 3.3 m, bulk membrane for high binding (150–160 µg/cm2) immunoblotting
Image
(Discontinued) Immun-Blot® PVDF Membrane, Precut, 7 x 8.4 cm
1620174
Pkg of 10, 0.2 µm, 7 x 8.4 cm, precut membrane for high binding capacity (150–160 µg/cm2) immunoblotting
Image
(Discontinued) Immun-Blot® PVDF Membrane, Precut, 10 x 15 cm
1620175
Pkg of 10, 0.2 µm, 10 x 15 cm, precut membrane for high binding capacity (150–160 µg/cm2) immunoblotting
Image
(Discontinued) Immun-Blot PVDF Membrane, Precut, 20 x 20 cm
1620176
Pkg of 10, 0.2 µm, 20 x 20 cm, precut membrane for high binding capacity (150–160 µg/cm2) immunoblotting
Image
(Discontinued) Immun-Blot® PVDF Membrane, Precut, 25 x 28 cm
1620255
Pkg of 10, 0.2 µm, 25 x 28 cm, precut membrane for high binding capacity (150–160 µg/cm2) immunoblotting
Image
(Discontinued) Immun-Blot® PVDF/Filter Paper Sandwiches
1620238
Pkg of 20, 8.5 x 13.5 cm, precut preassembled blotting membrane/filter paper sandwich for immunoblotting; fits Criterion™ and Criterion XT precast gels
Image
(Discontinued) Immun-Blot® PVDF/Filter Paper Sandwiches
1620239
Pkg of 50, 8.5 x 13.5 cm, precut preassembled blotting membrane/filter paper sandwich for immunoblotting; fits Criterion™ and Criterion XT precast gels
Image
(Discontinued) Immun-Blot PVDF/Filter Paper Sandwiches
1620218
Pkg of 20, 7 x 8.5 cm, precut preassembled blotting membrane/filter paper sandwich for immunoblotting; fits Mini-PROTEAN® and Ready Gel® precast gels
Image
(Discontinued) Immun-Blot PVDF/Filter Paper Sandwiches
1620219
Pkg of 50, 7 x 8.5 cm, precut preassembled blotting membrane/filter paper sandwich for immunoblotting; fits Mini-PROTEAN® and Ready Gel® precast gels
Documents
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Number Description Options
2895
Protein Blotting Guide, Ver C
2212
Application-Specific PVDF Membranes: Immun-Blot PVDF Membrane for Western Blotting and Sequi-Blot PVDF Membrane for Protein Sequencing, Rev C
4006127
Instruction Manual, Immun-Blot PVDF, Rev A