Immun-Star™ AP Chemiluminescence Kits

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Overview

The highly sensitive Immun-Star AP kit combines Bio-Rad's blotting reagents and expertise with CDP-Star chemiluminescence technology.
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Description

Description



Chemiluminescent western blot detection is a highly sensitive alternative to isotopic detection of proteins bound to blotting membranes. Instead of radioactively labeled antibodies, enzyme-conjugated secondary antibodies produce a light signal while acting on the substrate. An enhancer intensifies the signal output, enabling the detection of picogram quantities of antigen. Also, it increases the duration of the light signal up to 6–8 hr. The light signal can be captured on film or an imaging system. The light signal continues 24 hr after initial activation, which allows flexibility in obtaining data. Exposure times on film are typically 30 sec to 5 min, depending on sample amount and antibody specificity. The Immun-Star™ AP chemiluminescence kit is therefore a highly sensitive, fast-acting alternative to isotopic detection.

These kits combine Bio-Rad's blotting reagents and expertise with CDP-Star chemiluminescence technology. Additional features of Immun-Star AP kits include:

  • Working solution stability for 24 hr at room temperature
  • Compatibility with both PVDF and nitrocellulose membranes
  • Ability to reactivate a blot, even weeks later, with the addition of fresh chemiluminescent substrate
  • Detection of as little as 10 pg of protein, comparable to the sensitivity of radioactive methods
  • Possibility of stripping and reprobing

Two kits are available, based on goat anti-mouse or goat anti-rabbit conjugates.

 

Specifications

Specifications

Lower detection limit
Picogram
Signal duration
24 hr
Primary detection method
Film
Suggested antibody dilution
Primary: 1/1,000–1/6,000
Secondary: 1/3,000
Recommended membrane
Nitrocellulose or PVDF
Shelf life
12 months at 4°C

Ordering

Ordering

items

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1705018
125 ml, alkaline phosphatase substrate for 2,500 cm2 of membrane (~50 mini blots)
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1705010
Chemiluminescent western blot detection kit, includes substrate, enhancer, solutions, and AP secondary antibody conjugate; for 2,500 cm2 of membrane (~50 mini blots)
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1705011
Chemiluminescent western blot detection kit, includes substrate, enhancer, solutions, and AP secondary antibody conjugate; for 2,500 cm2 of membrane (~50 mini blots)
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1705012
Pkg of 1, alkaline phosphatase substrates and enhancer for 2,500 cm2 of membrane (~50 mini blots)
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1705014
Chemiluminescent western blot detection kit, includes substrate, enhancer, solutions, and AP secondary antibody conjugate, reagents; for 400 cm2 of membrane (~8 mini blots)
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1705015
Pkg of 1, TBS, Tween 20, nonfat dry milk for 2,500 cm2 of membrane (~50 mini blots)

Documents

Documents

Number Description Options
2032
Western Blotting Detection Reagents Brochure, Rev F
4006074
Instruction Manual, Immun-Star Chemiluminescent Protein Detection System, Rev D