Clarity and Clarity Max ECL Western Blotting Substrates

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Clarity & Clarity Max Western Blotting Substrates | Bio-Rad.com

Overview

Clarity Western ECL Substrate Performance

 Clarity Comparison

Comparison of chemiluminescent substrate sensitivity. A, five different western blots were loaded with the same protein loads of purified alcohol dehydrogenase (ADH). Blots were incubated with substrates per manufacturer recommendations, then imaged for 10 sec on the ChemiDoc™ MP Imaging System. B, the signal-to-noise ratio for each band is depicted in bar graph form. Clarity and ECL Plus provided the best signal-to-noise ratios across all protein loads.

Clarity Max Western ECL Substrate Performance

 Clarity Comparison

Comparison of chemiluminescent substrate signal intensity. A, five different western blots were loaded with the same protein loads of purified IKB alpha. Blots were incubated with substrates per manufacturer recommendations, then imaged for 70 sec on the ChemiDoc Touch Imaging System. B, the signal-to-noise ratios for the bands in the boxed area for Clarity Max, ECL Select, and SuperSignal West Femto are depicted in bar graph form. Clarity Max and ECL Select provided the best signal-to-noise ratios across all protein loads. ND, not determined; signal at 30 pg for SuperSignal West Femto was not detectable above background noise.

Chemiluminescence Chemistry

Chemiluminescence detection. The secondary antibody, which binds the antibody specific for the protein of interest, is conjugated to the horseradish peroxidase (HRP) enzyme. The HRP enzyme acts on a substrate such as luminol, releasing light. In Enhanced Chemiluminescence detection, enhancers are added to the substrate solution, greatly increasing the amount of light emitted and the duration the signal is generated. This greatly increases the sensitivity on western blots.

Clarity Western ECL Blotting Substrate is compatible with any HRP-conjugate secondary antibody and ideal for both digital and film-based imaging.
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Ordering Information


Description

Clarity Family of ECL Western Blotting Substrates

Enhanced chemiluminescence (ECL) is a highly sensitive method for detecting proteins bound to western blotting membranes. Bio-Rad’s Clarity family of ECL substrates provides simple, high-performance solutions for all your western blotting needs.

Clarity ECL Substrate is our most versatile chemiluminescence substrate yielding high signal, low background and a long signal duration. Clarity Max ECL Substrate is the substrate of choice for challenging blots that require maximum levels of sensitivity. Both substrates are compatible with any horseradish peroxidase (HRP) conjugate and ideal for all western blots and both digital and film-based imaging. Substrate components are stable at room temperature.

Clarity Western ECL Substrate

Use Clarity Western ECL Substrate with any HRP-conjugated secondary antibody for digital or film-based imaging of western blots.

Features and Benefits

  • Mid-femtogram-level sensitivity
  • 24-hour signal duration
  • 1-year shelf-life at room temperature
  • Ideal for digital and film-based imaging

Clarity ECL Substrate exhibits excellent visual limit of detection versus other commonly used ECL substrates on the market.


 

Clarity Max Western ECL Substrate

Use Clarity Max Western ECL Substrate with any HRP-conjugated secondary antibody for digital or film-based imaging of any western blots.

Features and Benefits

  • Low-femtogram-level sensitivity
  • Recommended for detection of low target amounts
  • Ideal for digital and film-based imaging

Clarity Max ECL Substrate exhibits superior visual limit of detection versus other commonly used high-performance ECL substrates on the market.


 

Packaging Options


Specifications

Lower detection limit Clarity: Mid-femtogram level
Clarity Max: Low-femtogram level
Signal duration Clarity: 24 hr
Clarity Max: 2 hr
Primary detection method Digital or film imaging
Suggested antibody dilution Primary: 1:1,000–1:50,000;
Secondary: 1:50,000–1:250,000
Recommended membrane Nitrocellulose, PVDF, or LF PVDF

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Clarity™ Western ECL Substrate, 20 ml

1705060S
Pkg of 1, contains 10 ml Clarity Western Peroxide Reagent and 10 ml Clarity Western Luminol/Enhancer Reagent

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$66.00 
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Clarity™ Western ECL Substrate, 200 ml

1705060
Pkg of 1, contains 100 ml Clarity Western Peroxide Reagent and 100 ml Clarity Western Luminol/Enhancer Reagent

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$157.00 
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Clarity Western ECL Substrate, 500 ml

1705061
Pkg of 1, contains 250 ml Clarity Western Peroxide Reagent and 250 ml Clarity Western Luminol/Enhancer Reagent

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$298.00 
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Clarity Max™ Western ECL Substrate, 20 ml

1705062S
Pkg of 1, contains 10 ml Clarity Max Western Peroxide Reagent and 10 ml Clarity Max Western Luminol/Enhancer Reagent

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$99.00 
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Clarity Max Western ECL Substrate, 100 ml

1705062
Pkg of 1, contains 50 ml Clarity Max Western Peroxide Reagent and 50 ml Clarity Max Luminol/Enhancer Reagent

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$349.00 
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Documents

Number Description Options
6305 Clarity and Clarity Max Western ECL Substrates Brochure Click to download [ Add to Cart (Free) ]
10026691 Clarity Western ECL Substrate Transition Guide, Rev A Click to download
10026527 Clarity™ and Clarity Max™ Western ECL Substrates Quick Start Guide, Ver D Click to download
10026389 Instruction Manual, Clarity™ and Clarity Max™ Western ECL Substrates, Ver C (D085075) Click to download
6148 Transfer of High Molecular Weight Proteins to Membranes: A Comparison of Transfer Efficiency Between Blotting Systems, Rev B Click to download
6493 Bio-Rad’s Host Cell Protein (HCP) Workflow Quick Guide Click to download [ Add to Cart (Free) ]

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