Trans-Blot Turbo Transfer System
The Trans-Blot Turbo system is a rapid protein transfer apparatus that can transfer protein to membrane in as little as 3 minutes. Trans-Blot Turbo transfer packs provide greater transfer efficiency in less time.
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Superior Transfer EfficiencyImage
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Trans-Blot Turbo System TourImage
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Trans-Blot Turbo System Instructional VideoImage
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Trans-Blot Turbo System vs. Traditional BlottingImage
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Trans-Blot Turbo System vs. iBlotImage
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Fast Transfers In As Little As 3 MinImage
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Better Transfer of Different Types of ProteinsImage
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Blotter ComparisonImage
Overview
The Trans-Blot Turbo Transfer System is a high-performance western blotting transfer system designed to provide rapid transfers with high efficiency. The system enables blot transfer of protein in as little as 3 minutes without sacrificing performance when compared with traditional tank protein blotting. The Trans-Blot Turbo Transfer Packs include an optimized buffer, membrane, and filter paper combination that provides superior blot transfers when paired with the Trans-Blot Turbo blotting apparatus. Protein blotting consumables are available in a ready-to-use, pre-packaged stack or in bulk quantities that require assembly.
Note: The Trans-Blot Turbo Transfer System is for research use only, not intended for diagnostic purposes
Features and Benefits of the Trans-Blot Turbo Transfer System
- Rapid blot transfer — 3 min transfers of mini or midi gels
- High throughput — can transfer 1–4 mini or 1–2 midi gels in a single run
- Greater transfer efficiency — higher transfer efficiency than other protein blotting methods
- Flexible design — allows the user to customize transfer conditions and is compatible with traditional semi-dry consumables
- Environmentally friendly — environmentally safe consumables eliminate disposal cost
- Recommended for Stain-Free Western Blotting — permits a streamlined workflow
Watch this short video to learn more about the Trans-Blot Turbo system.
Applications and Uses of the Trans-Blot Turbo Transfer System
This protein blotting system is a powerful tool in the western blotting workflow, enabling the visualization and identification of proteins for downstream analysis. The Trans-Blot Turbo transfer system can be used in the following applications:
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Start Tool- Western blotting
- Separation, transfer and analysis (Stain-Free Western Blotting Workflow)
- Protein blot transfers
- Evaluation of protein expression levels
- Immunological or biochemical analyses
- Protein-protein or protein-ligand interactions
Trans-Blot Turbo Transfer Packs
Trans-Blot Turbo transfer packs come with either PVDF or nitrocellulose membranes:
| Membrane Type and Pore Size | Preassembled Transfer Packs (10 Transfers) | RTA Transfer Packs (40 Transfers) | ||
|---|---|---|---|---|
| Mini | Midi | Mini | Midi | |
| 0.2 µm PVDF | 1704156 | 1704157 | 1704272 | 1704273 |
| 0.45 µm LF (low fluorescence) PVDF | – | – | 1704274 | 1704275 |
| 0.2 µm Nitrocellulose | 1704158 | 1704159 | 1704270 | 1704271 |
Select the Trans-Blot Turbo RTA transfer kit for a low-fluorescence membrane option.
Use Bio-Rad’s Precision Plus Protein WesternC Standards and Clarity Western ECL Substrate for chemiluminescence detection on both film and digital imagers. See how the Trans-Blot Turbo transfer system fits into the Stain-Free Western Blotting to streamline your western blotting process.
See the ordering information for Mini-PROTEAN TGX Precast Gels and Criterion TGX Precast Gels to select the best gel for your application.
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Blotting instrument, includes base, 2 cassettes to hold 1–2 midi or up to 4 mini blotting sandwiches, blot roller
Accessories
Kits & Reagents
1 Liter 5x Trans-Blot Turbo Transfer Buffer compatible with the TBT Ready-To-Assemble Transfer Kits
Ready-to-assemble transfer kit includes 40 mini-sized 0.2 µm nitrocellulose membranes (7 x 8.5 cm), 80 transfer stacks, 1 L 5x transfer buffer, and 2 gel trays for wetting and equilibrating membranes and transfer stacks
Ready-to-assemble transfer kit includes 40 midi-sized 0.2 µm nitrocellulose membranes (8.5 x 13.5 cm), 80 transfer stacks, 2 L 5x transfer buffer, and 2 gel trays for wetting and equilibrating membranes and transfer stacks
Ready-to-assemble transfer kit includes 40 midi-sized 0.45 µm LF PVDF membranes (8.5 x 13.5 cm), 80 transfer stacks, 2 L 5x transfer buffer, and 2 gel trays for wetting and equilibrating membranes and transfer stacks