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Transfer Systems | Membranes & Filter Paper | Buffers & Reagents | Fluorescent Western Blotting | Resources and Protocols | Related Products | Contact an Expert |
Western blotting is a powerful technique that allows you to positively detect your proteins, estimate quantities, and determine their molecular weights starting from a mixture of proteins extracted from cells or tissues.
Stain-Free Western Blotting
Stain-free western blotting allows you to quickly check electrophoresis and blot transfer quality and obtain truly quantitative western blotting results, updating traditional blotting techniques with innovative tools.
Faster Results. Better Data.
Download Stain-Free GuideWestern Blotting Transfer Systems
Trans-Blot Turbo Transfer System
The Trans-Blot Turbo System enables fast, efficient, and reproducible transfer of proteins up to 400 kD. Preassembled Trans-Blot Turbo Transfer Packs are optimized for superior blot transfer.
- Fast transfer, in as little as 3 min
- Highest efficiency transfer
- High throughput — 4 mini gels or 2 midi gels per run
- Recommended for Stain-Free Western Blotting
Semi-Dry and Rapid Blotting Systems
Wet/Tank Blotting Systems
- Mini Trans-Blot Cell — for 2 mini gels
- Criterion Blotter — for 2 midi or 4 mini gels
- Trans-Blot Cell — for 3 large, 6 midi, or 12 mini gels
- Trans-Blot Plus Cell — for 3 XL or 12 midi gels
Microfiltration and Screening Systems
Western Blotting Membranes & Filter Paper
Immun-Blot PVDF Membrane
This hydrophobic PVDF membrane is ideal for chemiluminescent and colorimetric western blots. High mechanical strength facilitates reprobing.
- Retains target proteins very strongly
- Reduces nonspecific protein binding
- Ensures high signal with low background
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Nitrocellulose Membranes
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PVDF Membranes
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Western Blotting Filter Paper
Western Blotting Buffers & Reagents
EveryBlot Blocking Buffer
- Complete western blot blocking in 5 minutes
- No incubation time for ELISA
- Low background and high signal on fluorescent and chemiluminescent blots
- Compatible with phosphorylated protein detection
Antibodies & Conjugates
- StarBright Blue 700 Fluorescent Antibodies
- hFAB Rhodamine Housekeeping Protein Fluorescent Antibodies
- PrecisionAb Validated Western Blotting Antibodies
- HRP and AP Conjugates
Detection Reagents
- Clarity and Clarity Max Western ECL Substrates
- Immun-Star AP Chemiluminescence Kits
- Immun-Blot Opti-4CN Colorimetric Kits
- Immun-Blot AP Colorimetric Kits
Buffers & Blocking Agents
FEATUREDFluorescent Western Blotting
Bio-Rad's fluorescent western blotting workflow is a seamless integration of products designed to work together to offer guaranteed results. This validated set of solutions will make it easy for you to get better data every time.
Resources
Free 26-Lane Electrophoresis & Western Blotting Layout Post-It Pad
Plan, track, and never miss another step.
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Quantitative Western Blotting: How to improve your data quality and reproducibility
Learn how to improve your western blotting results and generate publishable data. Follow along as a Bio-Rad western blotting expert walks you through techniques to improve the detection and quantification of proteins using western blotting.
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Can We Trust Western Blots?
In this presentation, Aldrin Gomes, Associate Professor of Neurobiology at UC Davis, talks about key success factors to instill trust in western blotting data.
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Western Blot Learning Center
The Western Blot Learning Center is a complete reference on all of the steps of western blotting, includes practical theory, protocols, and recommendations on how to make your blots better from experts.
Application Guides
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Western Blot Doctor Troubleshooting Guide
Our self-help troubleshooting guide covers solutions to many common and not-so-common western blotting issues and helps your blots look their best.
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Protein Blotting Guide
(PDF 7.9 MB)Details on blotting technology, available products, and methods, plus tips, techniques, and troubleshooting.
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Protein Electrophoresis Guide
(PDF 6.4 MB)A guide to polyacrylamide gel electrophoresis and protein detection, including theory, product selection, protocols, and more.
Protocols
Western Blotting Protocol Library
Filter by your laboratory set-up and reagents to get a custom western blotting protocol that best fits your needs.
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Receive Western Blotting Tips and Online Resource Updates
Keep up to date with useful tips to continuously improve your western blotting experiments from sample preparation through image analysis. Sign up to be the first to be notified when new western blotting resources like tips and tricks, posters, protocols, webinars, and how-to-videos become available.
Applications & Technologies
Western Blotting Techniques
Learn more about western blotting techniques. Find step-by-step protocols and helpful tips on equipment, membranes, transfer conditions, and detection methods.
Protein Separation and Analysis
Bio-Rad’s Stain-Free Western Blotting Workflow facilitates speed and validation at each step of a western blotting experiment — from running gels to quantifying proteins.
Protein Electrophoresis
Find protocols, video tutorials, and selection guides to help you at every step of your electrophoresis experiments.
Imaging and Analysis
Find information on protein visualization and quantitation methods, gel and blot imaging instrumentation, and image analysis software.
In-Cell Western Assays
In-Cell Western Assays are high throughput quantitative and qualitative immunofluorescence assays that utilizes Bio-Rad's ChemiDoc MP Imaging System and Image Lab Software.
Related Products
ChemiDoc Imaging Systems
ChemiDoc Imagers offer best-in-class performance with ease of use for fluorescence and chemiluminescence detection and all general gel documentation applications.
GelDoc Go Imaging System
Automated gel imaging and documentation for research laboratories and cGMP manufacturing environments.
GS-900 Calibrated Densitometer
Industry-friendly gel documentation system that calibrates itself before every run
(Note: This system is not enabled for stain-free detection)
Protein Ladders and Standards (Markers)
Prestained and unstained molecular weight standards for protein electrophoresis applications including SDS-PAGE, western blotting, 2-D PAGE, and isoelectric focusing (IEF).
Protein Gels
Find the right Bio-Rad protein gel for your application. Choose SDS-PAGE and native PAGE gels, convert to TGX Precast Gels, or choose specialized gel chemistries.
Electrophoresis Chambers
Our electrophoresis chambers enable rapid, high-resolution protein separation on precast or handcast gels over a variety of different gel sizes.
Protein Electrophoresis Buffers and Reagents
Loading buffers for protein or nucleic acid samples, detergents, ampholytes, and gel-casting buffers.
Power Supplies
From basic to high-voltage capacity, our power supplies provide simple programming in a compact, stackable case.
PrecisionAb Validated Western Blotting Antibodies
These premium antibodies are lab-validated using strict testing criteria to ensure superior performance in western blotting detection.