Fluorescent Western Blotting

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Imagine No Compromises — Move Beyond Traditional Imaging

With the ChemiDoc MP System, the superior quantitation of digital meets the sensitivity of film and delivers the best fluorescence performance with the brightest new fluorophore.

Traditional laser scanning systems and X-ray film are a thing of the past. Get higher multiplexing capabilities without sacrificing the performance you expect from a best-in-class imaging system.

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More Flexibility in Dye Selection

• Blue

  • DyLight 488
  • Alexa Fluor 488
  • Cy2

• Green

  • StarBright B520
  • DyLight 549
  • Alexa Fluor 546
  • Cy3
  • hFAB

• Red

  • DyLight 650
  • Alexa Fluor 647
  • Cy5
  • SYPRO Ruby

• Far Red

  • StarBright B700
  • DyLight 680
  • Alexa Fluor 680
  • Cy5.5
  • IRDye 680

• Near Infrared

  • DyLight 800
  • Alexa Fluor 790
  • Cy7
  • IRDye 800

10 Tips for Western Blot Detection of Phosphorylation Events

The Complete Capabilities of the ChemiDoc Imaging Systems: Superior Images

Better Options

With the ChemiDoc MP, one imaging system covers all of your gel documentation, chemiluminescence, and multiplex fluorescence needs.

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  Stain-Free
  Total Protein Normalization

  Learn how to properly design a quantitative western blotting experiment and faithfully compare protein expression levels, without concern for changing or overexpressed HKP’s.
  Learn More »about Stain-Free Total Protein Normalization

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  Chemiluminescence
  Replaces Film

  Save money on consumables and reduce environmental waste without compromising on performance. Get the sensitivity of film and the convenience of digital documentation.
  Learn More »about Comparing X-Ray Film Digital Imaging Systems

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  Multiplex Fluorescence
  3 Colors

  Save sample, avoid the errors commonly associated with stripping, reprobing, and cutting blots and answer more complex biological questions by optimizing your experiments to fluorescence.
  Learn More »about Total Protein Normalization

Better Data

See up to three signals at once or look at a subset of signals. Plus, you can normalize beyond a single protein with stain-free total protein normalization.

Please deselect stain-free to
explore blot combinations.

Explore blot combinations by making a selection on the right.

Better Experience

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Image Lab Touch Software

Image Lab Touch Software works with the ChemiDoc MP Imaging System and provides easy and intuitive onboard acquisition software. Built for labs with multiple users, Image Lab Touch Software teaches as you tap and explore, selects optimal light source, and permits both manual entry and automated calculation of exposure times.

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Intuitive Imaging at Your Fingertips

Get instructions showing how to capture images on the ChemiDoc MP System via Image Lab Touch Software
» View tutorials

Primary Antibodies

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  New Phospho-Specific PrecisionAb Antibodies

  • Highly specific antibodies to detect phosphorylation events
  • Validated by comparing phosphorylation levels in both treated versus untreated cell lysates and by dephosphorylating western blot membranes
  • Showcased against total protein antibodies
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  PrecisionAb Antibodies

  • Highly specific and sensitive
  • Validated for western blotting applications
  • Each antibody is screened against up to 12 whole-cell lysates expressing endogenous protein levels
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  hFAB Rhodamine Housekeeping Protein Fluorescent Primary Antibodies

  • One-step, directly conjugated, fluorescence detection solution for detecting commonly used HKP's (actin, tubulin, and GAPDH)
  • Eliminates cross-reactivity with primary and secondary antibodies from other species
  • HuCAL^® technology allows you to choose any appropriate host species for your other antibodies

Secondary Antibodies

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  StarBright Blue 700 Fluorescent Secondary Antibodies

  • Best choice for maximum sensitivity
  • Novel polymer-based chemistry is equivalent to femto ECL
  • Superior signal-to-noise ratio
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  StarBright Blue 520 Fluorescent Secondary Antibodies

  • Novel polymer-based chemistry is equivalent to femto ECL
  • Ideal for multiplexing
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  DyLight Fluorescent Secondary Antibodies

  • Traditional fluorescent solution
  • Available in many colors and host species
  • Ideal for routine fluorescent detection

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  Immun-Blot Low Fluorescence PVDF Membrane

  • Minimal autofluorescence allows long exposure times and maximizes the sensitivity of digital imaging systems
  • High protein retention and broad dynamic range enables more accurate quantitation
  • Compatible with most multiplex fluorescent blotting protocols
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  Pick the Best Standards for Your Blots!

  Just click on the light source in our interactive guide and see how banding pattern changes with light source. This interactive guide helps you pick the best protein standard for your gels and blots.

  Try Interactive Selection Guide

Western Workflow

Imaging Systems

Antibodies