
Fluorescent Western Blotting
Bio-Rad's fluorescent western blotting workflow is a seamless integration of products designed to work together to offer guaranteed results. This validated set of solutions will make it easy for you to get better data every time.
ChemiDoc MP Imaging System

Imagine No Compromises — Move Beyond Traditional Imaging
With the ChemiDoc MP System, the superior quantitation of digital meets the sensitivity of film and delivers the best fluorescence performance with the brightest new fluorophore.
Traditional laser scanning systems and X-ray film are a thing of the past. Get higher multiplexing capabilities without sacrificing the performance you expect from a best-in-class imaging system.
More Flexibility in Dye Selection
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Blue
- DyLight 488
- Alexa Fluor 488
- Cy2
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Green
- StarBright B520
- DyLight 549
- Alexa Fluor 546
- Cy3
- hFAB
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Red
- DyLight 650
- Alexa Fluor 647
- Cy5
- SYPRO Ruby
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Far Red
- StarBright B700
- DyLight 680
- Alexa Fluor 680
- Cy5.5
- IRDye 680
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Near Infrared
- DyLight 800
- Alexa Fluor 790
- Cy7
- IRDye 800
10 Tips for Western Blot Detection of Phosphorylation Events
The Complete Capabilities of the ChemiDoc Imaging Systems: Superior Images
Better Options
With the ChemiDoc MP, one imaging system covers all of your gel documentation, chemiluminescence, and multiplex fluorescence needs.
Stain-Free
Total Protein NormalizationLearn how to properly design a quantitative western blotting experiment and faithfully compare protein expression levels, without concern for changing or overexpressed HKP’s.
Learn More »about Stain-Free Total Protein NormalizationChemiluminescence
Replaces FilmSave money on consumables and reduce environmental waste without compromising on performance. Get the sensitivity of film and the convenience of digital documentation.
Learn More »about Comparing X-Ray Film Digital Imaging SystemsMultiplex Fluorescence
3 ColorsSave sample, avoid the errors commonly associated with stripping, reprobing, and cutting blots and answer more complex biological questions by optimizing your experiments to fluorescence.
Learn More »about Total Protein Normalization
Better Data
See up to three signals at once or look at a subset of signals. Plus, you can normalize beyond a single protein with stain-free total protein normalization.
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explore blot combinations.








Explore blot combinations by making a selection on the right.
Better Experience

Image Lab Touch Software
Image Lab Touch Software works with the ChemiDoc MP Imaging System and provides easy and intuitive onboard acquisition software. Built for labs with multiple users, Image Lab Touch Software teaches as you tap and explore, selects optimal light source, and permits both manual entry and automated calculation of exposure times.
Intuitive Imaging at Your Fingertips
Get instructions showing how to capture images on the ChemiDoc MP System via Image Lab Touch Software
» View tutorials

Stain-Free Western Blotting
Faster Results. Better Data.
Stain-free western blotting allows you to quickly check electrophoresis and blot transfer quality and obtain truly quantitative western blotting results, updating traditional blotting techniques with innovative tools.
Antibodies for Fluorescent Western Blotting
Primary Antibodies
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New Phospho-Specific PrecisionAb Antibodies
- Highly specific antibodies to detect phosphorylation events
- Validated by comparing phosphorylation levels in both treated versus untreated cell lysates and by dephosphorylating western blot membranes
- Showcased against total protein antibodies
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PrecisionAb Antibodies
- Highly specific and sensitive
- Validated for western blotting applications
- Each antibody is screened against up to 12 whole-cell lysates expressing endogenous protein levels
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hFAB Rhodamine Housekeeping Protein Fluorescent Primary Antibodies
- One-step, directly conjugated, fluorescence detection solution for detecting commonly used HKP's (actin, tubulin, and GAPDH)
- Eliminates cross-reactivity with primary and secondary antibodies from other species
- HuCAL® technology allows you to choose any appropriate host species for your other antibodies
Secondary Antibodies
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StarBright Blue 700 Fluorescent Secondary Antibodies
- Best choice for maximum sensitivity
- Novel polymer-based chemistry is equivalent to femto ECL
- Superior signal-to-noise ratio
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StarBright Blue 520 Fluorescent Secondary Antibodies
- Novel polymer-based chemistry is equivalent to femto ECL
- Ideal for multiplexing
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DyLight Fluorescent Secondary Antibodies
- Traditional fluorescent solution
- Available in many colors and host species
- Ideal for routine fluorescent detection
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Western Blot University
Courses designed to make you a western blotting expert.
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Introducing the Western Blot Learning Center
Perfect your western blotting. Learn from the experts.
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Free 26-Lane Electrophoresis & Western Blotting Layout Post-It Pad
Plan, track, and never miss another step.
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Western Blot Selector Tool
Find the right products for you using the free Western Blot Selector Tool
Blotting Membrane
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Immun-Blot Low Fluorescence PVDF Membrane
- Minimal autofluorescence allows long exposure times and maximizes the sensitivity of digital imaging systems
- High protein retention and broad dynamic range enables more accurate quantitation
- Compatible with most multiplex fluorescent blotting protocols
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Pick the Best Standards for Your Blots!
Just click on the light source in our interactive guide and see how banding pattern changes with light source. This interactive guide helps you pick the best protein standard for your gels and blots.
Benefits of Fluorescent Western Blotting
Learn more about the benefits of Fluorescent Western Blotting by checking out our video and document resources.

Light Up Your Lab
Get a free excitation/emission reference poster to make it easier to select the right fluorophore for your next experiment.
Documents
Western Workflow
Number | Description |
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PDF 3429 | The Complete Guide to Stain-Free Western Blotting |
PDF 6427 | Stain-Free Western Blotting Brochure |
PDF 6351 | Stain-Free Western Blotting Publication List |
Library | Western Blotting Protocol Library |
PDF 6390 | Stain-Free Western Blotting Protocol |
PDF RP0051 | Stain-Free Approach to Western Blotting |
Imaging Systems
Number | Description |
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PDF 6837 | ChemiDoc MP Brochure |
PDF 6936 | Summary of Features and Benefits |
Antibodies
Number | Description |
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PDF 6911 | StarBright Secondary Antibody Brochure |
PDF 10000078050 | StarBright Secondary Antibody Protocol |