Fluorescent Western Blotting

Introducing the Western Blot Learning Center

Perfect your western blotting. Learn from the experts.

Bio-Rad's fluorescent western blotting workflow is a seamless integration of products designed to work together to offer guaranteed results. This validated set of solutions will make it easy for you to get better data every time.

ChemiDoc MP Imaging System: Imagine No Compromises

With the ChemiDoc MP System, the superior quantitation of digital meets the sensitivity of film and delivers the best fluorescence performance with the brightest new fluorophore.

Move Beyond Traditional Imaging

Traditional laser scanning systems and X-ray film are a thing of the past. Get higher multiplexing capabilities without sacrificing the performance you expect from a best-in-class imaging system.

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More Flexibility in Dye Selection

Bio-Rad's ChemiDoc MP Fluorescent Scanner
 

ChemiDoc Imaging Systems

Complete Capabilities: Superior Images

Better Options

With the ChemiDoc MP, one imaging system covers all of your gel documentation, chemiluminescence, and multiplex fluorescence needs.

Stain-Free
Total Protein Normalization

 

Learn how to properly design a quantitative western blotting experiment and faithfully compare protein expression levels, without concern for changing or overexpressed HKP’s.
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Chemiluminescence
Replaces Film

 

Save money on consumables and reduce environmental waste without compromising on performance. Get the sensitivity of film and the convenience of digital documentation.
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Multiplex Fluorescence
3 Colors

 

Save sample, avoid the errors commonly associated with stripping, reprobing, and cutting blots and answer more complex biological questions by optimizing your experiments to fluorescence.
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Better Data

See up to three signals at once or look at a subset of signals. Plus, you can normalize beyond a single protein with stain-free total protein normalization.

Phospho-p44/42 MAPK (ERK1/2) — StarBright B700, Total p44/42 MAPK (ERK1/2) — DyLight 800, GAPDH — hFAB Rhodamine

Explore blot combinations by making a selection on the right.

Select Blots Below:








 

The stain-free channel is used in addition to the three other channels, but cannot be used at the same time. Learn more about stain-free here.

 

Better Experience

Image Lab Touch Software

Image Lab Touch Software works with the ChemiDoc MP Imaging System and provides easy and intuitive onboard acquisition software. Built for labs with multiple users, Image Lab Touch Software teaches as you tap and explore, selects optimal light source, and permits both manual entry and automated calculation of exposure times.

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Intuitive Imaging at Your Fingertips

Get instructions showing how to capture images on the ChemiDoc MP System via Image Lab Touch Software
» View tutorials

Exposure settings

Stain-Free Western Blotting

Stain-free western blotting allows you to quickly check electrophoresis and blot transfer quality and obtain truly quantitative western blotting results, updating traditional blotting techniques with innovative tools.

Faster Results. Better Data.

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Antibodies for Fluorescent Western Blotting

Primary Antibodies

Secondary Antibodies

NEW PRODUCT EveryBlot Blocking Buffer

The 5-minute solution for the best western blots.

See the Data

Blotting Membrane

Immun-Blot PVDF Membrane

Immun-Blot Low Fluorescence PVDF Membrane

  • Minimal autofluorescence allows long exposure times and maximizes the sensitivity of digital imaging systems
  • High protein retention and broad dynamic range enables more accurate quantitation
  • Compatible with most multiplex fluorescent blotting protocols
Interactive Selection Guide for Protein Standards

Pick the Best Standards for Your Blots!

Just click on the light source in our interactive guide and see how banding pattern changes with light source. This interactive guide helps you pick the best protein standard for your gels and blots. 

Try Interactive Selection Guide

Benefits of Fluorescent Western Blotting

Learn more about the benefits of Fluorescent Western Blotting by checking out our video and document resources.

Light Up Your Lab

Get a free excitation/emission reference poster to make it easier to select the right fluorophore for your next experiment.
» Request a Free Fluorophore Poster

Consult with an Specialist

Have Questions?

Contact a Specialist

Documents

  • ChemiDocMP Brochure
    (PDF 1.26 MB)

    Explore the features and benefits of the new high-end imaging system for the best fluorescence and chemiluminescence detection.

  • Summary of Features and Benefits
    (PDF 242 KB)

    See a summary of the features and benefits of the new ChemiDoc MP high-end imaging system.

Related Products

  • Western Blotting

    Western Blotting

    Western blotting products include the Stain-Free Western Blotting Workflow, protein transfer systems, blotting membranes, filter paper, premixed blotting buffers and reagents, and detection kits.

  • Antibodies

    Antibodies

    Find solutions for all your antibody needs: an extensive inventory of primary and secondary antibodies, controls, and reagents plus custom products including monoclonal generation in just 8 weeks.