EveryBlot Blocking Buffer

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EveryBlot Blocking Buffer


A five minute blocking buffer for ALL western blots.

EveryBlot Blocking Buffer provides 5 minute blocking and maximum sensitivity for all western blots regardless of detection method.

Features and Benefits

  • Reduces non-specific antibody binding to reduce background while maintaining excellent sensitivity
  • For both chemiluminescent and fluorescent detection
  • Contains no phosphate-based buffers. Ideal for use with phospho-specific antibodies
  • Complete blocking in 5 minutes

Recommended Protocol

For mini-sized blots, use at least 10 ml for blocking and antibody incubation steps. For midi-sized blots, use at least 20 ml.

  • Add block to the membrane and incubate for 5 minutes with agitation
  • Dilute primary and secondary antibodies in full-strength block and incubate for 1 hour with agitation

For fluorescent detection on PVDF, add SDS to 0.02% in the secondary antibody solution.

Fluorescent Detection

Clarity Western ECL Substrate

Fluorescent multiplex detection in HEK293 cells. After electrophoresis, proteins were transferred to low fluorescence PVDF. Left, blot blocked in a fluorescent optimized blocker for 1 hour. Right, blot blocked in EveryBlot Blocking Buffer for 5 minutes. After blocking, both blots processed identically and imaged to maximize signal and minimize background for each channel independently.

Chemiluminescent Detection

Clarity Max Western ECL Substrate

Chemiluminescent detection of three targets in HEK 293 cells. After blocking, both blots processed identically and imaged and displayed using identical parameters.

Phosphoprotein Detection

Compatibility

Nothing to interfere with phosphoprotein detection. Detection of total (green) and phospho-specific (red) targets. Left, Jurkat cells unstimulated (–) or stimulated (+) with calyculin A. Center and right, A431 cells unstimulated (–) or stimulated (+) with EGF.

Low Background across the Entire Spectrum

Low inherent fluorescence

Effect of blocking agent on membrane background. Low fluorescence PVDF membranes without sample or antibodies blocked in either a fluorescence optimized blocking buffer or EveryBlot Blocking Buffer. After washing, membranes were imaged side by side on the ChemiDoc MP Imaging System. Membrane autofluorescence was measured in each fluorescent channel. Other vendor's buffer (); EveryBlot Buffer ().

Packaging Options

Product Catalog Number
EveryBlot Blocking Buffer, 50 ml 12010947
EveryBlot Blocking Buffer, 500 ml 12010020

Related Products

  • 1x Tris Buffered Saline (TBS) with 1% Casein (1610782)
  • 1x Phosphate Buffered Saline (PBS) with 1% Casein (1610783)
  • Blotting-Grade Blocker (1706404)
  • Gelatin (1706537)

Related Categories

Supporting Documents

  • EveryBlot Blocking Buffer Flier
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EveryBlot Blocking Buffer, 500 ml

12010020
Bottle of blocking buffer, 500 ml. Requires only 5 minutes of blocking for all western blots

12010020 templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/12010020_1566949953.xml 12010020 08/27/19 11:40 PM en_US true internet/product_SKU 2019-12-02 21:27:07 /default/main/bio-rad/STAGING AR,BO,BR,CA,CL,CO,CR,DO,EC,SV,GU,GT,HT,HN,JM,MX,NI,PA,PY,PE,PR,TT,US,UY,VE,AU,BD,BN,KH,CN,HK,IN,ID,JP,KR,MO,MY,FM,NP,NZ,PW,PG,PH,SG,SB,LK,TW,TH,TO,VU,VN,AL,DZ,AI,AM,AT,AZ,BH,BE,BA,BW,BG,BF,CM,HR,CY,CZ,DK,EG,ER,EE,ET,FO,FI,FR,GF,PF,GA,GE,DE,GH,GR,GP,HU,IS,IE,IL,IT,JO,KZ,KE,XK,KW,LV,LB,LI,LT,LU,MK,MG,ML,MT,MQ,MU,MD,MS,MA,NL,NG,NO,OM,PK,PS,PL,PT,QA,RO,RU,ST,SA,SN,RS,SK,SI,ZA,ES,SE,CH,TZ,TG,TN,TR,UG,UA,AE,UK,UZ,VA,EH,YE templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/12010020_1566949953.xml /default/main/bio-rad/WORKAREA/default jzly4n8p 2019-08-27 23:52:33 true jzly4n8p templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/12010020_1566949953.xml LSR N /default/main/bio-rad en 09/02/29 10:39 AM 1573669965087 2019-12-02 21:27:07 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/12010020_1566949953.xml 12010020-everyblot-blocking-buffer-500-ml 1 12010020 EveryBlot Blocking Buffer 500 ml EveryBlot Blocking Buffer, 500 ml Bottle of blocking buffer, 500 ml. Requires only 5 minutes of blocking for all western blots Bottle of blocking buffer, 500 ml. Requires only 5 minutes of blocking for all western blots /webroot/web/images/lsr/products/electrophoresis/sku_thumb/global/12010020_th.jpg /webroot/web/images/lsr/products/electrophoresis/sku_view/global/12010020_view.jpg /webroot/web/images/lsr/products/electrophoresis/sku_view/global/12010020_view.jpg preview Jessica Low EveryBlot Blocking Buffer, 500 ml Bottle of blocking buffer, 500 ml. Requires only 5 minutes of blocking for all western blots blocking buffer, western blot, western blotting, immunoblot 08/27/19 11:40 PM 09/02/29 10:39 AM AR,BO,BR,CA,CL,CO,CR,DO,EC,SV,GU,GT,HT,HN,JM,MX,NI,PA,PY,PE,PR,TT,US,UY,VE,AU,BD,BN,KH,CN,HK,IN,ID,JP,KR,MO,MY,FM,NP,NZ,PW,PG,PH,SG,SB,LK,TW,TH,TO,VU,VN,AL,DZ,AI,AM,AT,AZ,BH,BE,BA,BW,BG,BF,CM,HR,CY,CZ,DK,EG,ER,EE,ET,FO,FI,FR,GF,PF,GA,GE,DE,GH,GR,GP,HU,IS,IE,IL,IT,JO,KZ,KE,XK,KW,LV,LB,LI,LT,LU,MK,MG,ML,MT,MQ,MU,MD,MS,MA,NL,NG,NO,OM,PK,PS,PL,PT,QA,RO,RU,ST,SA,SN,RS,SK,SI,ZA,ES,SE,CH,TZ,TG,TN,TR,UG,UA,AE,UK,UZ,VA,EH,YE LSR /LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl N 0 <!---<div class="right corner-box-promo-everyblot" style="width: 195px; margin: 0 0 20px 20px;"><a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/electrophoresis/product_overlay/global/everyblot_video.html')"><img src="/webroot/web/images/lsr/products/electrophoresis/product_detail/global/yt-everyblot-th.png" style="width: 195px; height: 94px; padding-left: 0px;" /></a><center><strong><a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/electrophoresis/product_overlay/global/everyblot_video.html')">A five minute blocking buffer for <span class="forcebreaks">ALL western blots.</span></a></strong></center></div>---> <div class="right"><center><a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/electrophoresis/product_overlay/global/everyblot_video.html')"><img src="/webroot/web/images/lsr/products/electrophoresis/product_detail/global/yt-everyblot-th2.png" /></a> <p>A five minute blocking buffer for ALL western blots.</p> </center></div> <p class="wraptxt">EveryBlot Blocking Buffer provides 5 minute blocking and maximum sensitivity for all western blots regardless of detection method.</p> <h2 class="descHeader">Features and Benefits</h2> <ul> <li>Reduces non-specific antibody binding to reduce background while maintaining excellent sensitivity</li> <li>For both chemiluminescent and fluorescent detection</li> <li>Contains no phosphate-based buffers. Ideal for use with phospho-specific antibodies</li> <li>Complete blocking in 5 minutes</li> </ul> <a name="fluorescentdetection"></a> <div class="breakoutfeature"> <h2>Recommended Protocol</h2> <div class="corner-box bg-white"> <p>For mini-sized blots, use at least 10 ml for blocking and antibody incubation steps. For midi-sized blots, use at least 20 ml.</p> <ul> <li>Add block to the membrane and incubate for 5 minutes with agitation</li> <li>Dilute primary and secondary antibodies in full-strength block and incubate for 1 hour with agitation</li> </ul> <p>For fluorescent detection on PVDF, add SDS to 0.02% in the secondary antibody solution.</p> </div> </div> <a name="fluorescentdetection"></a> <!---<div style="background-color: #f5db8c21; width: 650px;">---> <h2 class="descHeader bttm-border">Fluorescent Detection</h2> <img src="/webroot/web/images/lsr/products/electrophoresis/product_detail/global/low-background-high-signal-th.png" alt="Clarity Western ECL Substrate" class="top-margin" /><br /> <p><strong>Fluorescent multiplex detection in HEK293 cells.</strong> After electrophoresis, proteins were transferred to low fluorescence PVDF. Left, blot blocked in a fluorescent optimized blocker for 1 hour. Right, blot blocked in EveryBlot Blocking Buffer for 5 minutes. After blocking, both blots processed identically and imaged to maximize signal and minimize background for each channel independently.</p> <!---<div style="background-color: #47f4c921; width: 650px;">---> <div class="breakoutfeature bg-99ca3d30"> <h2 class="bttm-border">Chemiluminescent Detection</h2> <img src="/webroot/web/images/lsr/products/electrophoresis/product_detail/global/low-background-high-signal-chemiluminescence-th.png" alt="Clarity Max Western ECL Substrate" class="top-margin" /> <p><strong>Chemiluminescent detection of three targets in HEK 293 cells.</strong> After blocking, both blots processed identically and imaged and displayed using identical parameters.</p> </div> <!---<div style="background-color: rgba(156, 234, 156, 0.05); width: 650px;">---> <h2 class="descHeader bttm-border">Phosphoprotein Detection</h2> <center><img src="/webroot/web/images/lsr/products/electrophoresis/product_detail/global/compatible-phospho-detection.png" alt="Compatibility" width="auto" height="auto" class="top-margin" /></center> <p><strong>Nothing to interfere with phosphoprotein detection. </strong>Detection of total (green) and phospho-specific (red) targets. Left, Jurkat cells unstimulated (&ndash;) or stimulated (+) with calyculin A. Center and right, A431 cells unstimulated (&ndash;) or stimulated (+) with EGF.</p> <!---<div style="background-color: #eeececc2; width: 650px;">---> <div class="breakoutfeature bg-ffcc0029"> <h2 class="bttm-border">Low Background across the Entire Spectrum</h2> <img src="/webroot/web/images/lsr/products/electrophoresis/product_detail/global/low-inherent-fluorescence-th.png " alt="Low inherent fluorescence" /> <p><strong>Effect of blocking agent on membrane background.</strong> Low fluorescence PVDF membranes without sample or antibodies blocked in either a fluorescence optimized blocking buffer or EveryBlot Blocking Buffer. After washing, membranes were imaged side by side on the ChemiDoc MP Imaging System. Membrane autofluorescence was measured in each fluorescent channel. Other vendor's buffer (<img style="width: 8px; height: 8px; background-color: #999999;" src="/webroot/web/images/1x1.gif" alt="" />); EveryBlot Buffer (<img style="width: 8px; height: 8px; background-color: #090;" src="/webroot/web/images/1x1.gif" alt="" />).</p> </div> <h2 class="descHeader">Packaging Options</h2> <table class="pd_table pd_gridlines-alternate"> <thead> <tr> <th scope="col"><span class="left">Product</span></th> <th scope="col">Catalog Number</th> </tr> </thead> <tbody> <tr> <td>EveryBlot Blocking Buffer, 50 ml</td> <td><a href="/en-us/sku/12010947-everyblot-blocking-buffer-50-ml?ID=12010947">12010947</a></td> </tr> <tr> <td>EveryBlot Blocking Buffer, 500 ml</td> <td>12010020</td> </tr> </tbody> </table> <h2 class="descHeader">Related Products</h2> <ul> <li>1x Tris Buffered Saline (TBS) with 1% Casein (<a href="/en-us/sku/1610782-1x-tris-buffered-saline-tbs-with-1-casein?ID=1610782">1610782</a>)</li> <li>1x Phosphate Buffered Saline (PBS) with 1% Casein (<a href="/en-us/sku/1610783-1x-phosphate-buffered-saline-pbs-with-1-casein?ID=1610783">1610783</a>)</li> <li>Blotting-Grade Blocker (<a href="/en-us/sku/1706404-blotting-grade-blocker?ID=1706404">1706404</a>)</li> <li>Gelatin (<a href="/en-us/sku/1706537-gelatin?ID=1706537">1706537</a>)</li> </ul> <h2 class="descHeader">Related Categories</h2> <ul> <li><a href="/en-us/category/western-blotting?ID=9324fd3c-6af4-4551-831e-8db6fa3f3452-membranes-filter-paper">Western Blotting Membranes</a></li> <li><a href="/en-us/category/immunodetection-reagents-kits?ID=534c009d-cd86-4b33-956c-674d5a6ae054">Immunodetection Reagents and Kits</a></li> <li><a href="/en-us/category/western-blotting?ID=9324fd3c-6af4-4551-831e-8db6fa3f3452">Western Blotting Products</a></li> <li><a href="/en-us/category/protein-electrophoresis-blotting">Protein Electrophoresis and Blotting</a></li> </ul> <h2 class="descHeader">Supporting Documents</h2> <ul> <li>EveryBlot Blocking Buffer <a href="/webroot/web/pdf/lsr/literature/Bulletin_7185.pdf" target="_blank" rel="noopener noreferrer" class="no-target-style">Flier</a></li> </ul> /webroot/web/html/lsr/products/electrophoresis/sku_view/description/global 12010020.html /webroot/web/html/lsr/products/electrophoresis/sku_view/description/global/12010020.html Storage temperature 2212 4°C 1 0 Shelf life 2149 At least 6 months 1 0 Detection method 2749 Colorimetric, chemiluminescence, fluorescence 1 0 Notes 2814 * low backround and high signal on fluorescent and chemiluminescent blots</br> * Compatible with phosphorylated protein detection</br> * Complete blocking in 5 minutes</br> 1 0 7185 templatedata/internet/documentation/data/LSR/Literature/7185_1569886526.xml 1706516 templatedata/internet/product_SKU/data/ja_JP/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/170-6516_0921120403104.xml 1706515 templatedata/internet/product_SKU/data/ja_JP/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/170-6515_0921120403177.xml 1705060 templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/170-5060_1343333743.xml 1705062 templatedata/internet/product_SKU/data/ja_JP/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/1705062_1453861114.xml 12004158 templatedata/internet/product_SKU/data/ja_JP/LSR/Antibodies/12004158-_1486421410.xml 12005866 templatedata/internet/product_SKU/data/ja_JP/LSR/Antibodies/12005866_1519172223.xml false $125.00 false $ null true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=12010020&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=12010020&CartQuote=QT&quantity= 1178 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=12010020&AddToDefaultWishList=true&quantity= 41105300 125.00 12010020
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EveryBlot Blocking Buffer, 50 ml

12010947
Bottle of blocking buffer, 50 ml. Requires only 5 minutes of blocking for all western blots

12010947 templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/12010947_1566950242.xml 12010947 08/27/19 11:54 PM en_US true internet/product_SKU 2019-12-02 21:26:55 /default/main/bio-rad/STAGING AR,BO,BR,CA,CL,CO,CR,DO,EC,SV,GU,GT,HT,HN,JM,MX,NI,PA,PY,PE,PR,TT,US,UY,VE,AU,BD,BN,KH,CN,HK,IN,ID,JP,KR,MO,MY,FM,NP,NZ,PW,PG,PH,SG,SB,LK,TW,TH,TO,VU,VN,AL,DZ,AI,AM,AT,AZ,BH,BE,BA,BW,BG,BF,CM,HR,CY,CZ,DK,EG,ER,EE,ET,FO,FI,FR,GF,PF,GA,GE,DE,GH,GR,GP,HU,IS,IE,IL,IT,JO,KZ,KE,XK,KW,LV,LB,LI,LT,LU,MK,MG,ML,MT,MQ,MU,MD,MS,MA,NL,NG,NO,OM,PK,PS,PL,PT,QA,RO,RU,ST,SA,SN,RS,SK,SI,ZA,ES,SE,CH,TZ,TG,TN,TR,UG,UA,AE,UK,UZ,VA,EH,YE templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/12010947_1566950242.xml /default/main/bio-rad/WORKAREA/default jzly4n8q 2019-08-27 23:57:23 true jzly4n8q templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/12010947_1566950242.xml LSR N /default/main/bio-rad en 08/27/37 01:55 PM 1573580156638 2019-12-02 21:26:55 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/12010947_1566950242.xml 12010947-everyblot-blocking-buffer-50-ml 1 12010947 EveryBlot Blocking Buffer 50 ml EveryBlot Blocking Buffer, 50 ml Bottle of blocking buffer, 50 ml. Requires only 5 minutes of blocking for all western blots Bottle of blocking buffer, 50 ml. Requires only 5 minutes of blocking for all western blots /webroot/web/images/lsr/products/electrophoresis/sku_thumb/global/12010947_th.jpg /webroot/web/images/lsr/products/electrophoresis/sku_view/global/12010947_view.jpg preview Jessica Low EveryBlot Blocking Buffer, 50 ml Bottle of blocking buffer, 50 ml. Requires only 5 minutes of blocking for all western blots 12010947, blocking buffer, western blotting, western blot, immunoblot 08/27/19 11:54 PM 08/27/37 01:55 PM AR,BO,BR,CA,CL,CO,CR,DO,EC,SV,GU,GT,HT,HN,JM,MX,NI,PA,PY,PE,PR,TT,US,UY,VE,AU,BD,BN,KH,CN,HK,IN,ID,JP,KR,MO,MY,FM,NP,NZ,PW,PG,PH,SG,SB,LK,TW,TH,TO,VU,VN,AL,DZ,AI,AM,AT,AZ,BH,BE,BA,BW,BG,BF,CM,HR,CY,CZ,DK,EG,ER,EE,ET,FO,FI,FR,GF,PF,GA,GE,DE,GH,GR,GP,HU,IS,IE,IL,IT,JO,KZ,KE,XK,KW,LV,LB,LI,LT,LU,MK,MG,ML,MT,MQ,MU,MD,MS,MA,NL,NG,NO,OM,PK,PS,PL,PT,QA,RO,RU,ST,SA,SN,RS,SK,SI,ZA,ES,SE,CH,TZ,TG,TN,TR,UG,UA,AE,UK,UZ,VA,EH,YE LSR /LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl N 0 <!---<div class="corner-box corner-box-promo corner-box-promo-everyblot linkpointer" style="margin-bottom: 5px; height: 94px; width: 195px; padding: 0px;"><a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/electrophoresis/product_overlay/global/everyblot_video.html')"><img src="/webroot/web/images/lsr/products/electrophoresis/product_detail/global/yt-everyblot-th.png" style="width: 195px; height: 94px; padding-left: 0px;" /></a></div> <p style="float: right; padding-right: 0px; padding-left: 20px; width: 195px;"><strong><a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/electrophoresis/product_overlay/global/everyblot_video.html')">A five minute blocking buffer for ALL western blots.</a></strong></p> ---> <div class="right"><center><a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/electrophoresis/product_overlay/global/everyblot_video.html')"><img src="/webroot/web/images/lsr/products/electrophoresis/product_detail/global/yt-everyblot-th2.png" /></a> <p>A five minute blocking buffer for ALL western blots.</p> </center></div> <p class="wraptxt">EveryBlot Blocking Buffer provides 5 minute blocking and maximum sensitivity for all western blots regardless of detection method.</p> <h2 class="descHeader">Features and Benefits</h2> <ul> <li>Reduces non-specific antibody binding to reduce background while maintaining excellent sensitivity</li> <li>For both chemiluminescent and fluorescent detection</li> <li>Contains no phosphate-based buffers. Ideal for use with phospho-specific antibodies</li> <li>Complete blocking in 5 minutes</li> </ul> <a name="fluorescentdetection"></a> <div class="breakoutfeature"> <h2>Recommended Protocol</h2> <div class="corner-box bg-white"> <p>For mini-sized blots, use at least 10 ml for blocking and antibody incubation steps. For midi-sized blots, use at least 20 ml.</p> <ul> <li>Add block to the membrane and incubate for 5 minutes with agitation</li> <li>Dilute primary and secondary antibodies in full-strength block and incubate for 1 hour with agitation</li> </ul> <p>For fluorescent detection on PVDF, add SDS to 0.02% in the secondary antibody solution.</p> </div> </div> <a name="fluorescentdetection"></a> <!---<div style="background-color: #f5db8c21; width: 650px;">---> <h2 class="descHeader bttm-border">Fluorescent Detection</h2> <img src="/webroot/web/images/lsr/products/electrophoresis/product_detail/global/low-background-high-signal-th.png" alt="Clarity Western ECL Substrate" class="top-margin" /><br /> <p><strong>Fluorescent multiplex detection in HEK293 cells.</strong> After electrophoresis, proteins were transferred to low fluorescence PVDF. Left, blot blocked in a fluorescent optimized blocker for 1 hour. Right, blot blocked in EveryBlot Blocking Buffer for 5 minutes. After blocking, both blots processed identically and imaged to maximize signal and minimize background for each channel independently.</p> <!---<div style="background-color: #47f4c921; width: 650px;">---> <div class="breakoutfeature bg-99ca3d30"> <h2 class="bttm-border">Chemiluminescent Detection</h2> <img src="/webroot/web/images/lsr/products/electrophoresis/product_detail/global/low-background-high-signal-chemiluminescence-th.png" alt="Clarity Max Western ECL Substrate" class="top-margin" /> <p><strong>Chemiluminescent detection of three targets in HEK 293 cells.</strong> After blocking, both blots processed identically and imaged and displayed using identical parameters.</p> </div> <!---<div style="background-color: rgba(156, 234, 156, 0.05); width: 650px;">---> <h2 class="descHeader bttm-border">Phosphoprotein Detection</h2> <center><img src="/webroot/web/images/lsr/products/electrophoresis/product_detail/global/compatible-phospho-detection.png" alt="Compatibility" width="auto" height="auto" class="top-margin" /></center> <p><strong>Nothing to interfere with phosphoprotein detection. </strong>Detection of total (green) and phospho-specific (red) targets. Left, Jurkat cells unstimulated (&ndash;) or stimulated (+) with calyculin A. Center and right, A431 cells unstimulated (&ndash;) or stimulated (+) with EGF.</p> <!---<div style="background-color: #eeececc2; width: 650px;">---> <div class="breakoutfeature bg-ffcc0029"> <h2 class="bttm-border">Low Background across the Entire Spectrum</h2> <img src="/webroot/web/images/lsr/products/electrophoresis/product_detail/global/low-inherent-fluorescence-th.png " alt="Low inherent fluorescence" /> <p><strong>Effect of blocking agent on membrane background.</strong> Low fluorescence PVDF membranes without sample or antibodies blocked in either a fluorescence optimized blocking buffer or EveryBlot Blocking Buffer. After washing, membranes were imaged side by side on the ChemiDoc MP Imaging System. Membrane autofluorescence was measured in each fluorescent channel. Other vendor's buffer (<img style="width: 8px; height: 8px; background-color: #999999;" src="/webroot/web/images/1x1.gif" alt="" />); EveryBlot Buffer (<img style="width: 8px; height: 8px; background-color: #090;" src="/webroot/web/images/1x1.gif" alt="" />).</p> </div> <h2 class="descHeader">Packaging Options</h2> <table class="pd_table pd_gridlines-alternate"> <thead> <tr> <th scope="col"><span class="left">Product</span></th> <th scope="col">Catalog Number</th> </tr> </thead> <tbody> <tr> <td>EveryBlot Blocking Buffer, 50 ml</td> <td>12010947</td> </tr> <tr> <td>EveryBlot Blocking Buffer, 500 ml</td> <td><a href="/en-us/sku/12010020-everyblot-blocking-buffer-500-ml?ID=12010020">12010020</a></td> </tr> </tbody> </table> <h2 class="descHeader">Related Products</h2> <ul> <li>1x Tris Buffered Saline (TBS) with 1% Casein (<a href="/en-us/sku/1610782-1x-tris-buffered-saline-tbs-with-1-casein?ID=1610782">1610782</a>)</li> <li>1x Phosphate Buffered Saline (PBS) with 1% Casein (<a href="/en-us/sku/1610783-1x-phosphate-buffered-saline-pbs-with-1-casein?ID=1610783">1610783</a>)</li> <li>Blotting-Grade Blocker (<a href="/en-us/sku/1706404-blotting-grade-blocker?ID=1706404">1706404</a>)</li> <li>Gelatin (<a href="/en-us/sku/1706537-gelatin?ID=1706537">1706537</a>)</li> </ul> <h2 class="descHeader">Related Categories</h2> <ul> <li><a href="/en-us/category/western-blotting?ID=9324fd3c-6af4-4551-831e-8db6fa3f3452-membranes-filter-paper">Western Blotting Membranes</a></li> <li><a href="/en-us/category/immunodetection-reagents-kits?ID=534c009d-cd86-4b33-956c-674d5a6ae054">Immunodetection Reagents and Kits</a></li> <li><a href="/en-us/category/western-blotting?ID=9324fd3c-6af4-4551-831e-8db6fa3f3452">Western Blotting Products</a></li> <li><a href="/en-us/category/protein-electrophoresis-blotting">Protein Electrophoresis and Blotting</a></li> </ul> <h2 class="descHeader">Supporting Documents</h2> <ul> <li>EveryBlot Blocking Buffer <a href="/webroot/web/pdf/lsr/literature/Bulletin_7185.pdf" target="_blank" rel="noopener noreferrer" class="no-target-style">Flier</a></li> </ul> /webroot/web/html/lsr/products/electrophoresis/sku_view/description/global 12010947.html /webroot/web/html/lsr/products/electrophoresis/sku_view/description/global/12010947.html Storage temperature 2212 4°C 1 0 Shelf life 2149 At least 6 months 1 0 Detection method 2749 Colorimetric, chemiluminescence, fluorescence 1 0 Notes 2814 * low backround and high signal on fluorescent and chemiluminescent blots</br> * Compatible with phosphorylated protein detection</br> * Complete blocking in 5 minutes</br> 1 0 7185 templatedata/internet/documentation/data/LSR/Literature/7185_1569886526.xml 1706516 templatedata/internet/product_SKU/data/ja_JP/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/170-6516_0921120403104.xml 1706515 templatedata/internet/product_SKU/data/ja_JP/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/170-6515_0921120403177.xml 1705060 templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/170-5060_1343333743.xml 1705062 templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/1705062_1453861114.xml 12004158 templatedata/internet/product_SKU/data/ja_JP/LSR/Antibodies/12004158-_1486421410.xml 12005866 templatedata/internet/product_SKU/data/ja_JP/LSR/Antibodies/12005866_1519172223.xml false $25.00 false $ null true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=12010947&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=12010947&CartQuote=QT&quantity= 1178 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=12010947&AddToDefaultWishList=true&quantity= 41105300 25.00 12010947
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