Overview
A five minute blocking buffer for ALL western blots.
Bio-Rad's Western Blot Blocking Reagents are designed to minimize non-specific binding during immunodetection, ensuring clear and accurate results. The flagship product, EveryBlot Blocking Buffer, offers complete membrane blocking in just 5 minutes, enhancing sensitivity across all western blot applications, including both chemiluminescent and fluorescent detection methods. Notably, it is free from phosphate-based buffers, making it ideal for use with phospho-specific antibodies.
In addition to EveryBlot Blocking Buffer, Bio-Rad provides a variety of blocking reagents to suit different experimental needs:
- PBS with 1% Casein
- TBS with 1% Casein
- Gelatin
- Nonfat Dry Milk Blotting-Grade Blocker
These reagents are essential for researchers seeking to achieve optimal blocking conditions tailored to their specific western blotting protocols.
| Product | Catalog Number | Description |
| EveryBlot Blocking Buffer, 500 ml | 12010020 | Bottle of blocking buffer, 500 ml |
| PBS with 1% Casein | 1610783 | Blocking reagent, 1 L |
| TBS with 1% Casein | 1610782 | Blocking reagent, 1 L |
| Gelatin | 1706537 | Enzyme immunoassay grade gelatin, 200 g |
| Blotting-Grade Blocker | 1706404 | Nonfat dry milk, 300 g |
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Bottle of blocking buffer, 500 ml. Requires only 5 minutes of blocking for all western blots and eliminates incubation time in ELISA assays.
1 L, blocking reagent
1 L, blocking reagent
100 ml, enzyme immunoassay grade polysorbate surfactant (detergent)
1 L, detergent, for easy pipetting
