Overview
A five minute blocking buffer for ALL western blots.
Proper blocking of membranes before immunodetection is critical for reducing non-specific binding. An effective blocker minimizes background and allows sensitive detection of target proteins.
EveryBlot Blocking Buffer
EveryBlot Blocking Buffer provides 5 minute blocking and maximum sensitivity for all western blots regardless of detection method.
Features and Benefits
- Reduces non-specific antibody binding to reduce background while maintaining excellent sensitivity
- For both chemiluminescent and fluorescent detection
- Contains no phosphate-based buffers. Ideal for use with phospho-specific antibodies
- Complete blocking in 5 minutes
| Product | Catalog Number | Description |
| EveryBlot Blocking Buffer, 500 ml | 12010020 | Bottle of blocking buffer, 500 ml |
| EveryBlot Blocking Buffer, 50 ml | 12010947 | Bottle of blocking buffer, 50 ml |
| PBS with 1% Casein | 1610783 | Blocking reagent, 1 L |
| TBS with 1% Casein | 1610782 | Blocking reagent, 1 L |
| Gelatin | 1706537 | Enzyme immunoassay grade gelatin, 200 g |
| Blotting-Grade Blocker | 1706404 | Nonfat dry milk, 300 g |
Ordering
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(Discontinued) EveryBlot Blocking Buffer, 500 ml
12010020
Bottle of blocking buffer, 500 ml. Requires only 5 minutes of blocking for all western blots
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(Discontinued) Tween 20, 100% Nonionic Detergent
1706531
100 ml, enzyme immunoassay grade polysorbate surfactant (detergent)
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(Discontinued) 10% Tween 20, Nonionic Detergent
1610781
1 L, detergent, for easy pipetting
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(Discontinued) 20% SDS Solution, 1 L
1610418
1 L, 20% (w/v) sodium dodecyl sulfate (SDS) solution
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(Discontinued) 10% SDS Solution, 250 ml
1610416
250 ml, 10% (w/v) sodium dodecyl sulfate (SDS) solution