Western Blot Blocking Reagents

Western Blot Blocking Reagents

Proper blocking of membranes before immunodetection is critical for reducing non-specific binding. An effective blocker minimizes background and allows sensitive detection of target proteins.

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Overview

A five minute blocking buffer for ALL western blots.

Bio-Rad's Western Blot Blocking Reagents are designed to minimize non-specific binding during immunodetection, ensuring clear and accurate results. The flagship product, EveryBlot Blocking Buffer, offers complete membrane blocking in just 5 minutes, enhancing sensitivity across all western blot applications, including both chemiluminescent and fluorescent detection methods. Notably, it is free from phosphate-based buffers, making it ideal for use with phospho-specific antibodies.

In addition to EveryBlot Blocking Buffer, Bio-Rad provides a variety of blocking reagents to suit different experimental needs:

  • PBS with 1% Casein
  • TBS with 1% Casein
  • Gelatin
  • Nonfat Dry Milk Blotting-Grade Blocker

These reagents are essential for researchers seeking to achieve optimal blocking conditions tailored to their specific western blotting protocols.

Product Catalog Number Description
EveryBlot Blocking Buffer, 500 ml 12010020 Bottle of blocking buffer, 500 ml
PBS with 1% Casein 1610783 Blocking reagent, 1 L
TBS with 1% Casein 1610782 Blocking reagent, 1 L
Gelatin 1706537 Enzyme immunoassay grade gelatin, 200 g
Blotting-Grade Blocker 1706404 Nonfat dry milk, 300 g

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12010020

Bottle of blocking buffer, 500 ml. Requires only 5 minutes of blocking for all western blots and eliminates incubation time in ELISA assays.