Western Blot Blocking Reagents

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Overview

Proper blocking of membranes before immunodetection is critical for reducing non-specific binding. An effective blocker minimizes background and allows sensitive detection of target proteins.

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Description

Description

Proper blocking of membranes before immunodetection is critical for reducing non-specific binding. An effective blocker minimizes background and allows sensitive detection of target proteins.

EveryBlot Blocking Buffer

A blocking buffer that enables 5 minute blocking and maximum sensitivity for all western blots regardless of detection method.

Features and Benefits

  • Complete blocking in 5 minutes
  • For both chemiluminescent and fluorescent detection
  • Contains no phosphate-based buffers. Ideal for use with phospho-specific antibodies
Product Catalog Number Description
EveryBlot Blocking Buffer, 500 ml 12010020 Bottle of blocking buffer, 500 ml
EveryBlot Blocking Buffer, 50 ml 12010947 Bottle of blocking buffer, 50 ml
PBS with 1% Casein 1610783 Blocking reagent, 1 L
TBS with 1% Casein 1610782 Blocking reagent, 1 L
Gelatin 1706537 Enzyme immunoassay grade gelatin, 200 g
Blotting-Grade Blocker 1706404 Nonfat dry milk, 300 g

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12010020
Bottle of blocking buffer, 500 ml. Requires only 5 minutes of blocking for all western blots
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12010947
Bottle of blocking buffer, 50 ml. Requires only 5 minutes of blocking for all western blots
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1706531

100 ml, enzyme immunoassay grade polysorbate surfactant (detergent)

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1610781
1 L, detergent, for easy pipetting
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1610418
1 L, 20% (w/v) sodium dodecyl sulfate (SDS) solution
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1610416
250 ml, 10% (w/v) sodium dodecyl sulfate (SDS) solution