Western Blot Blocking Reagents

Western Blot Blocking Reagents

Overview

Proper blocking of membranes before immunodetection is critical for reducing non-specific binding. An effective blocker minimizes background and allows sensitive detection of target proteins.

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Description

Description

competitor-everyblot-5 minute everyblot blocking

A five minute blocking buffer for ALL western blots.

Proper blocking of membranes before immunodetection is critical for reducing non-specific binding. An effective blocker minimizes background and allows sensitive detection of target proteins.

EveryBlot Blocking Buffer

EveryBlot Blocking Buffer provides 5 minute blocking and maximum sensitivity for all western blots regardless of detection method.

Features and Benefits

  • Reduces non-specific antibody binding to reduce background while maintaining excellent sensitivity
  • For both chemiluminescent and fluorescent detection
  • Contains no phosphate-based buffers. Ideal for use with phospho-specific antibodies
  • Complete blocking in 5 minutes
Product Catalog Number Description
EveryBlot Blocking Buffer, 500 ml 12010020 Bottle of blocking buffer, 500 ml
EveryBlot Blocking Buffer, 50 ml 12010947 Bottle of blocking buffer, 50 ml
PBS with 1% Casein 1610783 Blocking reagent, 1 L
TBS with 1% Casein 1610782 Blocking reagent, 1 L
Gelatin 1706537 Enzyme immunoassay grade gelatin, 200 g
Blotting-Grade Blocker 1706404 Nonfat dry milk, 300 g

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12010020

Bottle of blocking buffer, 500 ml. Requires only 5 minutes of blocking for all western blots