Western Blotting
Western blotting is a powerful technique that allows you to positively detect your proteins, estimate quantities, and determine their molecular weights starting from a mixture of proteins extracted from cells or tissues.
Western Blot Selector Tool
Planning western blotting experiments but don't know what gel type to use? Unsure about what blotting membrane works best and how to visualize your results? The Western Blotting Selection Tool is here to help you.
Stain-Free Western Blotting
Stain-Free technology is superior to conventional western blotting methods as it allows users to:
- Visualize gels minutes after electrophoresis
- Perform quality assessments at each step without the need for additional staining and destaining
- Increase accuracy and produce reliable quantitative results via total protein normalization
Western Blotting Transfer Systems
Trans-Blot Turbo Transfer System
The Trans-Blot Turbo System enables fast, efficient, and reproducible transfer of proteins up to 400 kD. Preassembled Trans-Blot Turbo Transfer Packs are optimized for superior blot transfer.
- Fast transfer, in as little as 3 min
- Highest efficiency transfer
- High throughput — 4 mini gels or 2 midi gels per run
- Recommended for Stain-Free Western Blotting
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Semi-Dry and Rapid Blotting Systems
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Wet/Tank Blotting Systems
- Mini Trans-Blot Cell — for 2 mini gels
- Criterion Blotter — for 2 midi or 4 mini gels
- Trans-Blot Cell — for 3 large, 6 midi, or 12 mini gels
- Trans-Blot Plus Cell — for 3 XL or 12 midi gels
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Microfiltration and Screening Systems
Trans-Blot Turbo Transfer Membranes & Filter Paper
Trans-Blot Turbo Transfer Membranes & Filter Paper
Trans-Blot Turbo Transfer Membranes and Filter Paper are available in PVDF, nitrocellulose, and low-fluor PVDF, and filter paper combinations that provide superior blot transfers when paired with the Trans-Blot Turbo Blotting Apparatus.
- Optimal for all western blot detection methods (chemiluminescent, fluorescent, and colorimetric)
- Compatible with the Stain-Free Western Blotting Workflow
- High signal with low background
- Efficient – 3-minute transfer when paired with the Trans-Blot Turbo Transfer System
- Available in three formats (preassembled, ready-to-assemble, or individual components)
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Nitrocellulose Membranes
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PVDF Membranes
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Western Blotting Filter Paper
Western Blotting Buffers & Reagents
EveryBlot Blocking Buffer
- Complete western blot blocking in 5 minutes
- No incubation time for ELISA
- Low background and high signal on fluorescent and chemiluminescent blots
- Compatible with phosphorylated protein detection
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Antibodies & Conjugates
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Detection Reagents
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Buffers & Blocking Agents
Fluorescent Western Blotting
Bio-Rad's fluorescent western blotting workflow is a seamless integration of products designed to work together to offer guaranteed results. This validated set of solutions will make it easy for you to get better data every time.
- Detect multiple targets at once: Fluorescent multiplexing enables simultaneous detection of multiple proteins on the same blot
- Save time and reduce costs: Eliminate the need for stripping and reprobing membranes
- Streamlined workflow: User-friendly software simplifies data collection and analysis
Resources
Featured WebinarBlot Talk: Monthly Live Webinar Series.
Join our live Blot Talk webinars to troubleshoot real western blots submitted by you, discover the latest techniques and advancements, and learn essential best practices. Get your questions answered live and gain practical advice to get the best results from your western blots every session!
Featured WebinarQuantitative Western Blotting: How to Improve Your Data Quality and Reproducibility
Learn how to improve your western blotting results and generate publishable data. Follow along as a Bio-Rad western blotting expert walks you through techniques to improve the detection and quantification of proteins using western blotting.
Featured WebinarCan We Trust Western Blots?
In this presentation, Aldrin Gomes, Associate Professor of Neurobiology at UC Davis, talks about key success factors to instill trust in western blotting data.
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Western Blot Learning Center
The Western Blot Learning Center is a complete reference on all of the steps of western blotting, includes practical theory, protocols, and recommendations on how to make your blots better from experts.
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Western Blot University
Courses designed to make you a western blotting expert.
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Western Blot Doctor Troubleshooting Guide
Our self-help troubleshooting guide covers solutions to many common and not-so-common western blotting issues and helps your blots look
their best.
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Protein Blotting Guide
(PDF 7.9 MB)Details on blotting technology, available products, and methods, plus tips, techniques, and troubleshooting.
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Protein Electrophoresis Guide
(PDF 6.4 MB)A guide to polyacrylamide gel electrophoresis and protein detection, including theory, product selection, protocols, and more.
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Better Western Blotting Guide
Tips, Techniques, and Technologies from the Western Blotting Experts at Bio-Rad Laboratories
Western Blotting Protocol Library
Filter by your laboratory set-up and reagents to get a custom western blotting protocol that best fits your needs.
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Total Protein Normalization Publication List (PDF 197 KB)
Browse a selection of research articles that highlights the advantages of Stain-Free western blotting
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Western Blotting Instrument and Reagent Guide (PDF 2.8 MB)
Navigate instrument and reagent options for Stain-Free Western Blotting with ease using the Western Blotting Instrument and Reagent Guide.
Free 26-Lane Electrophoresis & Western Blotting Layout Post-It Pad
Plan, track, and never miss another step.
Join the Fun
Get your free protein standard temporary tattoo, or get 5 for you and your lab mates.
Receive Western Blotting Tips & Online Resource Updates
Sign Up to Be the First to Know
Keep up to date with useful tips to continuously improve your western blotting experiments from sample preparation through image analysis. Sign up to be the first to be notified when new western blotting resources like tips and tricks, posters, protocols, webinars, and how-to-videos become available.
Applications & Technologies
Western Blotting Techniques
Learn more about western blotting techniques. Find step-by-step protocols and helpful tips on equipment, membranes, transfer conditions, and detection methods.
Protein Separation & Analysis
Bio-Rad’s Stain-Free Western Blotting Workflow facilitates speed and validation at each step of a western blotting experiment — from running gels to quantifying proteins.
Protein Electrophoresis
Find protocols, video tutorials, and selection guides to help you at every step of your electrophoresis experiments.
Imaging & Analysis
Find information on protein visualization and quantitation methods, gel and blot imaging instrumentation, and image analysis software.
In-Cell Western Assays
In-Cell Western Assays are high throughput quantitative and qualitative immunofluorescence assays that utilizes Bio-Rad's ChemiDoc MP Imaging System and Image Lab Software.
