Western Blotting

Find the right products for you using the free Western Blot Selector Tool

Western blotting is a powerful technique that allows you to positively detect your proteins, estimate quantities, and determine their molecular weights starting from a mixture of proteins extracted from cells or tissues.

Stain-Free Western Blotting

Stain-free western blotting allows you to quickly check electrophoresis and blot transfer quality and obtain truly quantitative western blotting results, updating traditional blotting techniques with innovative tools.

Faster Results. Better Data.

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Western Blotting Transfer Systems

Trans-Blot Turbo Transfer System

Trans-Blot Turbo Transfer System

The Trans-Blot Turbo System enables fast, efficient, and reproducible transfer of proteins up to 400 kD. Preassembled Trans-Blot Turbo Transfer Packs are optimized for superior blot transfer.

  • Fast transfer, in as little as 3 min
  • Highest efficiency transfer
  • High throughput — 4 mini gels or 2 midi gels per run
  • Recommended for Stain-Free Western Blotting

Semi-Dry and Rapid Blotting Systems

Wet/Tank Blotting Systems

Microfiltration and Screening Systems


Western Blotting Membranes & Filter Paper

Immun-Blot® PVDF Membrane

Immun-Blot PVDF Membrane

This hydrophobic PVDF membrane is ideal for chemiluminescent and colorimetric western blots. High mechanical strength facilitates reprobing.

  • Retains target proteins very strongly
  • Reduces nonspecific protein binding
  • Ensures high signal with low background

Western Blotting Buffers & Reagents

EveryBlot Blocking Buffer

EveryBlot Blocking Buffer

  • Complete blocking in 5 minutes
  • Low background and high signal on fluorescent and chemiluminescent blots
  • Compatible with phosphorylated protein detection

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FEATUREDFluorescent Western Blotting

Bio-Rad's fluorescent western blotting workflow is a seamless integration of products designed to work together to offer guaranteed results. This validated set of solutions will make it easy for you to get better data every time.

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Free 26-Lane Electrophoresis & Western Blotting Layout Post-It Pad

Plan, track, and never miss another step.

  • Quantitative Western Blotting: How to improve your data quality and reproducibility

    Learn how to improve your western blotting results and generate publishable data. Follow along as a Bio-Rad western blotting expert walks you through techniques to improve the detection and quantification of proteins using western blotting.

  • Can We Trust Western Blots?

    In this presentation, Aldrin Gomes, Associate Professor of Neurobiology at UC Davis, talks about key success factors to instill trust in western blotting data.

  • courses

    Western Blot Learning Center

    The Western Blot Learning Center is a complete reference on all of the steps of western blotting, includes practical theory, protocols, and recommendations on how to make your blots better from experts. 

Western Blot University

Courses designed to make you a western blotting expert.

Application Guides

  • Western Blot Dr. Troubleshooting Guide

    Western Blot Doctor Troubleshooting Guide

    Our self-help troubleshooting guide covers solutions to many common and not-so-common western blotting issues and helps your blots look their best.

  • Protein Blotting Guide

    Protein Blotting Guide
    (PDF 7.9 MB)

    Details on blotting technology, available products, and methods, plus tips, techniques, and troubleshooting.

  • Electrophoresis Guide

    Protein Electrophoresis Guide
    (PDF 6.4 MB)

    A guide to polyacrylamide gel electrophoresis and protein detection, including theory, product selection, protocols, and more.

Better Western Blotting Guide

Tips, Techniques, and Technologies from the Western Blotting Experts at Bio-Rad Laboratories

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Keep up to date with useful tips to continuously improve your western blotting experiments from sample preparation through image analysis. Sign up to be the first to be notified when new western blotting resources like tips and tricks, posters, protocols, webinars, and how-to-videos become available.

Applications & Technologies

  • Western Blotting Techniques

    Western Blotting Techniques

    Learn more about western blotting techniques. Find step-by-step protocols and helpful tips on equipment, membranes, transfer conditions, and detection methods.

  • Protein Separation and Analysis

    Protein Separation and Analysis

    Bio-Rad’s Stain-Free Western Blotting Workflow facilitates speed and validation at each step of a western blotting experiment — from running gels to quantifying proteins.

  • Protein Electrophoresis

    Protein Electrophoresis

    Find protocols, video tutorials, and selection guides to help you at every step of your electrophoresis experiments.

  • Imaging and Analysis

    Imaging and Analysis

    Find information on protein visualization and quantitation methods, gel and blot imaging instrumentation, and image analysis software.

  • In-Cell Western Assays

    In-Cell Western Assays

    In-Cell Western Assays are high throughput quantitative and qualitative immunofluorescence assays that utilizes Bio-Rad's ChemiDoc MP Imaging System and Image Lab Software.

Related Products

  • Chemidoc Imaging Systems

    ChemiDoc Imaging Systems

    ChemiDoc Imagers offer best-in-class performance with ease of use for fluorescence and chemiluminescence detection and all general gel documentation applications.

  • GelDoc Go Imaging System

    GelDoc Go Imaging System

    Automated gel imaging and documentation for research laboratories and cGMP manufacturing environments.

  • GS-900 Calibrated Densitometer

    GS-900 Calibrated Densitometer

    Industry-friendly gel documentation system that calibrates itself before every run

    (Note: This system is not enabled for stain-free detection)

  • Protein Ladders and Standards (Markers)

    Protein Ladders and Standards (Markers)

    Prestained and unstained molecular weight standards for protein electrophoresis applications including SDS-PAGE, western blotting, 2-D PAGE, and isoelectric focusing (IEF).

  • Protein Gels

    Protein Gels

    Find the right Bio-Rad protein gel for your application. Choose SDS-PAGE and native PAGE gels, convert to TGX Precast Gels, or choose specialized gel chemistries.

  • Electrophoresis Chambers

    Electrophoresis Chambers

    Our electrophoresis chambers enable rapid, high-resolution protein separation on precast or handcast gels over a variety of different gel sizes.

  • Protein Electrophoresis Buffers and Reagents

    Protein Electrophoresis Buffers and Reagents

    Loading buffers for protein or nucleic acid samples, detergents, ampholytes, and gel-casting buffers.

  • Power Supplies

    From basic to high-voltage capacity, our power supplies provide simple programming in a compact, stackable case.

  • PrecisionAb Validated Western Blotting Antibodies

    PrecisionAb Validated Western Blotting Antibodies

    These premium antibodies are lab-validated using strict testing criteria to ensure superior performance in western blotting detection.