SsoAdvanced™ Universal Probes Supermix

Print
SsoAdvanced Universal Probes Supermix

Overview

PCR Inhibitor Tolerance

Superior PCR inhibitor tolerance with SsoAdvanced universal probes supermix. A GAPDH qPCR assay with an input of 50 ng cDNA was performed with 1, 2, 2.5, and 3 µM hematin spiked in the cDNA to evaluate tolerance. The SsoAdvanced universal probes supermix tolerated greater levels of inhibition compared to the competitor mix. Cq, quantification cycle.


GC-Rich and AT-Rich qPCR

Optimal qPCR efficiency and sensitivity with SsoAdvanced™ universal probes supermix. A, a 72% GC-rich CBP assay, with an input of 100 ng–10 pg cDNA, was performed on a CFX96™ real-time PCR detection system and produced standard curve R2 = 0.997 and efficiency = 97%; B, a 71% AT-rich APC assay, with an input of 500 ng–50 pg gDNA, was performed on an Applied Biosystems 7500 system and produced standard curve R2 = 0.998 and efficiency = 98%. RFU, relative fluorescence units; ΔRn, baseline-corrected normalized reporter.


Duplex Reaction

Superior qPCR performance in a duplex reaction with SsoAdvanced™ universal probes supermix. Predesigned TaqMan gene expression assays in a duplex reaction with 10 ng–100 fg cDNA, using an 18S rRNA assay (), and 100 ng–10 pg cDNA, using a TFRC assay (), were performed on an Applied Biosystems ViiA 7 system. 18S rRNA efficiency = 98%; TFRC efficiency = 101%. ΔRn, baseline-corrected normalized reporter.


Long Amplicon and GC-Rich Duplex Reaction
Long Amplicon and GC-Rich Duplex Reaction

Duplex reaction with SsoAdvanced™ universal probes supermix. A duplex reaction with 100 ng–100 fg cDNA, using GAPDH 226 bp () and 72% GC-rich CBP () Taq targets, was performed on a CFX96™ real-time PCR detection system. GAPDH efficiency = 99.7%; CBP efficiency = 102%. RFU, relative fluorescence units.


Single-Copy Detection
Long Amplicon and GC-Rich Duplex Reaction

Single-copy detection with SsoAdvanced™ universal probes supermix. A gDNA assay targeting IL-1β, with gDNA serially diluted to a calculated single copy (), was performed on a CFX96™ real-time PCR detection system. RFU, relative fluorescence units.


Our Universal Real-Time PCR Reagents Web App brings you in-depth product information, individual and competitive performance data, interactive tutorials, 3D animated videos, technical manuals and more.

SsoAdvanced universal probes supermix is a high-performance real-time PCR supermix based on Bio-Rad's patented* Sso7d fusion protein technology. This supermix is formulated for a wide range of real-time PCR applications and for use with all ROX dependent or independent real-time PCR systems. The dsDNA binding protein, Sso7d, stabilizes the polymerase-template complex, providing superior inhibitor tolerance, increased processivity, specificity, and greater speed without affecting PCR sensitivity, efficiency, or reproducibility.

SsoAdvanced universal probes supermix lets you:

  • Carry out high-performance singleplex and duplex reactions — the Sso7d fusion polymerase and advanced formulation enable robust performance in singleplex or duplex real-time PCR reactions, providing the highest data precision and allowing cost and time savings when combining 2 assays in a single well
  • Use any real-time PCR system — the universal reference dye in this supermix enables ROX normalization of quantitative PCR (qPCR) data regardless of the ROX level requirements of the qPCR system
  • Achieve superior real-time PCR results under various conditions — our robust formulation delivers consistent performance in fast cycling across a broad range of reaction conditions, primer concentrations, and temperature ranges
  • Increase your qPCR sensitivity and efficiency of detection from compromised samples — the Sso7d fusion polymerase has increased resistance to a wide variety of PCR inhibitors, which helps provide better sensitivity and overall performance
  • Decrease time to results without compromising qPCR data quality — the Sso7d fusion polymerase and optimized buffer together provide rapid polymerization kinetics and instant polymerase activation

Applications and Uses of SsoAdvanced Universal Probes Supermix

  • qPCR/real-time PCR
  • Gene expression analysis
  • Absolute quantification
  • Genotyping (allelic discrimination)
  • Mutation detection
  • Pathogen detection
  • Viral detection (load)
  • Characterization of genetically modified organisms (GMO)
  • Genetic profiling

Instrument Compatibility

The SsoAdvanced universal probes supermix is compatible with all commercially available and all Bio-Rad real-time qPCR systems.

Related Products

We have developed SsoAdvanced™ universal SYBR® Green supermix, which is available in several formats (200–5,000 reactions).

Bio-Rad offers a complete workflow solution for real-time PCR, including:

More Information

To ensure that your qPCR reactions are reproducible and reliable, follow our step-by-step design and optimization protocols. We also provide insights into analysis of qPCR data and troubleshooting reactions.

* U.S. patents 6,627,424; 7,541,170; and 7,560,260.

Product expiration (after shipping date) 12 months
Storage at 4°C (after thawing) Up to 3 months
Customer guarantee (minimum usage time) at –20°C 12 months
SsoAdvanced™ Universal Probes Supermix

172-5280
2 ml (2 x 1 ml vials), 2x qPCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, stabilizers, ROX normalization dyes, for 200 x 20 µl reactions

List Price:   $145.00
Your Price:   Log in
Quantity:   Add to cart
Add to Hot List   Add to Quote
SsoAdvanced™ Universal Probes Supermix

172-5281
5 ml (5 x 1 ml vials), 2x qPCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, stabilizers, ROX normalization dyes, for 500 x 20 µl reactions

List Price:   $325.00
Your Price:   Log in
Quantity:   Add to cart
Add to Hot List   Add to Quote
SsoAdvanced™ Universal Probes Supermix

172-5282
10 ml (10 x 1 ml vials), 2x qPCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, stabilizers, ROX normalization dyes, for 1,000 x 20 µl reactions

List Price:   $625.00
Your Price:   Log in
Quantity:   Add to cart
Add to Hot List   Add to Quote
SsoAdvanced Universal Probes Supermix
SsoAdvanced™ Universal Probes Supermix

172-5284
25 ml (5 x 5 ml vials), 2x qPCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, stabilizers, ROX normalization dyes, for 2,500 x 20 µl reactions

List Price:   $1,460.00
Your Price:   Log in
Quantity:   Add to cart
Add to Hot List   Add to Quote
SsoAdvanced™ Universal Probes Supermix

172-5285
50 ml (10 x 5 ml vials), 2x qPCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, stabilizers, ROX normalization dyes, for 5,000 x 20 µl reactions

List Price:   $2,785.00
Your Price:   Log in
Quantity:   Add to cart
Add to Hot List   Add to Quote
Number Description Options
10031340 Instruction Manual, SsoAdvanced™ Universal Probes Supermix, Rev A Click to download
10023650 SsoAdvanced™ Universal Probes Supermix Product Insert, Rev A Click to download
6252 Reagent Comparison Guide for Real-Time PCR Brochure, Rev C Click to download
6090 Amplification Reagents and Plastics Brochure, Rev D Click to download [ Add to Cart (Free) ]