iTaq Universal Probes Supermix

Allows challenging simplex and duplex qPCR for a wide variety of gene expression analysis targets on any real-time PCR instrument.

Ordering Information

Overview

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iTaq universal probes supermix is a 2x concentrated, ready-to-use reaction master mix containing all components, except primers, probes, and template, for probe-based quantitative PCR (qPCR; simplex or duplex reaction) on any real-time PCR instrument. It contains antibody-mediated hot-start iTaq DNA polymerase, dNTPs, MgCl₂, enhancers, stabilizers, and a blend of passive reference dyes (including ROX).

iTaq universal probes supermix lets you:

Perform challenging simplex or duplex assays for a wide variety of targets
Obtain reliable and reproducible gene expression analysis results with any fluorogenic probe
Achieve consistent results across real-time instrument platforms under fast or standard conditions

iTaq universal probes supermix contains:

Advanced buffer formulation for optimal probe-based qPCR
Antibody-mediated hot-start iTaq DNA polymerase for rapid activation (30 sec)
Blend of passive reference dyes for universal real-time instrument compatibility

Instrument Compatibility

iTaq Universal Probes Supermix is compatible with Bio-Rad Real-Time PCR Detection Systems and commercially available systems listed below:

Systems

Applied Biosystems	StepOne/StepOnePlus 7500, ViiA 7 7000, 7300, 7700, 7900HT, QuantStudio Systems
Stratagene	Mx3000P, 3005P, 4000
Eppendorf	Mastercycler ep realplex 2, 4
QIAGEN	Corbett Rotor-Gene 3000, 6000, Q
Roche	LightCycler 480, 96 LightCycler 1.0, 1.5, 2.0*
BioFire	LightScanner HR-1 LightScanner 32*

* BSA must be added according to instrument specifications

Applications and Uses

Gene expression analysis
Genotyping
Mutation detection
Pathogen detection

Specifications

Storage at –20°C

Guaranteed for 12 months protected from light in a constant temperature freezer

Repeated freeze/thaw cycles

Up to 10 times

Storage at 4°C

Guaranteed for 3 months protected from light

Polymerase

iTaq DNA polymerase

Hot-start mechanism

Antibody mediated

Hot-start activation

95°C, 30 sec

Supermix concentration

Thermal cycling protocol

Standard and fast

Instrument compatibility

Validated on all Bio-Rad and ROX-dependent Applied Biosystems real-time PCR instruments and Roche LightCycler 480, QIAGEN Rotor-Gene Q, Eppendorf Mastercycler ep realplex, and Stratagene Mx real-time PCR systems

Template compatibility

cDNA, genomic DNA, plasmid DNA

Detection method

Fluorogenic probes

Ordering

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(Discontinued) iTaq™ Universal Probes Supermix, 200 x 20 µl rxns, 2 ml (2 x 1 ml)

1725130

200 x 20 µl reactions, 2 ml (2 x 1 ml), 2x qPCR mix, contains dNTPs, iTaq DNA polymerase, MgCl₂, enhancers, stabilizers, and ROX normalization dyes

(Discontinued) iTaq™ Universal Probes Supermix, 500 x 20 µl rxns, 5 ml (5 x 1 ml)

1725131

500 x 20 µl reactions, 5 ml (5 x 1 ml), 2x qPCR mix, contains dNTPs, iTaq DNA Polymerase, MgCl₂, enhancers, stabilizers, and ROX Normalization Dyes

(Discontinued) iTaq™ Universal Probes Supermix, 1,000 x 20 µl rxns, 10 ml (10 x 1 ml)

1725132

1,000 x 20 µl reactions, 10 ml (10 x 1 ml), 2x qPCR mix, contains dNTPs, iTaq DNA polymerase, MgCl₂, enhancers, stabilizers, and ROX normalization dyes

(Discontinued) iTaq™ Universal Probes Supermix, 2,500 x 20 µl rxns, 25 ml (5 x 5 ml)

1725134

2,500 x 20 µl reactions, 25 ml (5 x 5 ml), 2x qPCR mix, contains dNTPs, iTaq DNA polymerase, MgCl₂, enhancers, stabilizers, and ROX normalization dyes

(Discontinued) iTaq™ Universal Probes Supermix, 5,000 x 20 µl rxns, 50 ml (10 x 5 ml)

1725135

5,000 x 20 µl reactions, 50 ml (10 x 5 ml), 2x qPCR mix, contains dNTPs, iTaq DNA polymerase, MgCl₂, enhancers, stabilizers, and ROX normalization dyes

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