Notifications
View all notifications

No notifications to display.

Check back here for the latest on your quotes, orders, and accounts.

iTaq Universal Probes Supermix

iTaq Universal Probes Supermix

Allows challenging simplex and duplex qPCR for a wide variety of gene expression analysis targets on any real-time PCR instrument.

Read more
Ordering information

Overview

CFX Loyalty Program

Earn a free one-year service contract on your CFX System with purchase of Reagents.

CFX Loyalty Program

Our Universal Real-Time PCR Reagents Web App brings you in-depth product information, individual and competitive performance data, interactive tutorials, 3D animated videos, technical manuals and more.

iTaq universal probes supermix is a 2x concentrated, ready-to-use reaction master mix containing all components, except primers, probes, and template, for probe-based quantitative PCR (qPCR; simplex or duplex reaction) on any real-time PCR instrument. It contains antibody-mediated hot-start iTaq DNA polymerase, dNTPs, MgCl2, enhancers, stabilizers, and a blend of passive reference dyes (including ROX).

iTaq universal probes supermix lets you:

iTaq universal probes supermix contains:

  • Advanced buffer formulation for optimal probe-based qPCR
  • Antibody-mediated hot-start iTaq DNA polymerase for rapid activation (30 sec)
  • Blend of passive reference dyes for universal real-time instrument compatibility

Instrument Compatibility

iTaq Universal Probes Supermix is compatible with Bio-Rad Real-Time PCR Detection Systems and commercially available systems listed below:

Systems

Applied Biosystems
  • StepOne/StepOnePlus
  • 7500, ViiA 7
  • 7000, 7300, 7700, 7900HT, QuantStudio Systems
Stratagene
  • Mx3000P, 3005P, 4000
Eppendorf
  • Mastercycler ep realplex 2, 4
QIAGEN
  • Corbett Rotor-Gene 3000, 6000, Q
Roche
  • LightCycler 480, 96
  • LightCycler 1.0, 1.5, 2.0*
BioFire
  • LightScanner HR-1
  • LightScanner 32*

* BSA must be added according to instrument specifications

Applications and Uses

  • Gene expression analysis
  • Genotyping
  • Mutation detection
  • Pathogen detection

Specifications

Storage at –20°C
Guaranteed for 12 months protected from light in a constant temperature freezer
Repeated freeze/thaw cycles
Up to 10 times
Storage at 4°C
Guaranteed for 3 months protected from light
Polymerase
iTaq DNA polymerase
Hot-start mechanism
Antibody mediated
Hot-start activation
95°C, 30 sec
Supermix concentration
2x
Thermal cycling protocol
Standard and fast
Instrument compatibility
Validated on all Bio-Rad and ROX-dependent Applied Biosystems real-time PCR instruments and Roche LightCycler 480, QIAGEN Rotor-Gene Q, Eppendorf Mastercycler ep realplex, and Stratagene Mx real-time PCR systems
Template compatibility
cDNA, genomic DNA, plasmid DNA
Detection method
Fluorogenic probes

Ordering

items

Use the filters below to refine results!

(Discontinued) iTaq™ Universal Probes Supermix, 200 x 20 µl rxns, 2 ml (2 x 1 ml)
1725130
200 x 20 µl reactions, 2 ml (2 x 1 ml), 2x qPCR mix, contains dNTPs, iTaq DNA polymerase, MgCl2, enhancers, stabilizers, and ROX normalization dyes
(Discontinued) iTaq™ Universal Probes Supermix, 500 x 20 µl rxns, 5 ml (5 x 1 ml)
1725131
500 x 20 µl reactions, 5 ml (5 x 1 ml), 2x qPCR mix, contains dNTPs, iTaq DNA Polymerase, MgCl2, enhancers, stabilizers, and ROX Normalization Dyes
(Discontinued) iTaq™ Universal Probes Supermix, 1,000 x 20 µl rxns, 10 ml (10 x 1 ml)
1725132
1,000 x 20 µl reactions, 10 ml (10 x 1 ml), 2x qPCR mix, contains dNTPs, iTaq DNA polymerase, MgCl2, enhancers, stabilizers, and ROX normalization dyes
(Discontinued) iTaq™ Universal Probes Supermix, 2,500 x 20 µl rxns, 25 ml (5 x 5 ml)
1725134
2,500 x 20 µl reactions, 25 ml (5 x 5 ml), 2x qPCR mix, contains dNTPs, iTaq DNA polymerase, MgCl2, enhancers, stabilizers, and ROX normalization dyes
(Discontinued) iTaq™ Universal Probes Supermix, 5,000 x 20 µl rxns, 50 ml (10 x 5 ml)
1725135
5,000 x 20 µl reactions, 50 ml (10 x 5 ml), 2x qPCR mix, contains dNTPs, iTaq DNA polymerase, MgCl2, enhancers, stabilizers, and ROX normalization dyes

Documents

  • Add to Cart (Free)
  • Add to Cart (Free)
  • Add to Cart (Free)