iScript Advanced cDNA Synthesis Kit for RT-qPCR

Image
lsr_iscript_advanced_cdna.jpg

Overview

Allows increased data throughput (high capacity up to 7.5 µg input RNA) from a single 20 µl reverse transcription reaction. This two-component cDNA synthesis kit features a short 21-minute protocol.
Read full description Ordering Information

Description

Description

The iScript Advanced cDNA Synthesis Kit for RT-qPCR is an enhanced formulation that delivers increased data throughput from a single 20 µl reverse transcription (RT) reaction for real-time qPCR. This two-tube kit enables superior RT capacity of input RNA up to 7.5 µg.

Key Features and Benefits

  • Increase qPCR data throughput and cost effectiveness from a single reaction — cDNA synthesized from higher input RNA amounts can be used for analyzing large numbers of target genes
  • Detect low-level target genes — uncompromised sensitivity even with less input RNA for when RNA sample is limited
  • Reduce inter-assay variability and improve reproducibility — eliminate the need for multiple cDNA synthesis reactions
  • Allow true representation of target mRNA levels — potent blend of RNase inhibitor prevents RNA degradation during reaction setup and RT
  • Increase primer design flexibility and prevent 5′ and 3′ bias — optimum blend of oligo(dT) and random primers for superior RNA coverage
  • Set up reactions with ease and obtain cDNA in less time — 2-tube kit generates cDNA in 21 minutes

Specifications

Specifications

Storage at –20°C
Guaranteed for 12 months at –20°C in a constant temperature freezer (multiple freeze thaws not recommended)

Ordering

Ordering

items

Use the filters below to refine results!

170-8842_tn.jpg
1725037
25 x 20 µl reactions, includes 100 µl iScript Advanced 5x Reaction Mix, 25 µl iScript Advanced Reverse Transcriptase
170-8842_tn.jpg
1725038
100 x 20 µl reactions, includes 400 µl 5x iScript Advanced Reaction Mix, 100 µl iScript Advanced Reverse Transcriptase
1725038bun_th.jpg
1725038BUN
500 x 20 µl reactions, includes 5 x 400 µl 5x iScript Advanced Reaction Mix, 5 x 100 µl iScript Advanced Reverse Transcriptase

Documents

Documents

Number Description Options
10000070791
iScript™ Advanced cDNA Synthesis Kit for RT-qPCR Product Insert, Ver B
6746
iScript Reverse Transcription Kits Flier
[ Add to Cart (Free) ]
6252
Reagent Comparison Guide for Real-Time PCR Brochure, Rev C
[ Add to Cart (Free) ]
6090
Amplification Reagents and Plastics Brochure
[ Add to Cart (Free) ]
6763
Gene Expression Real-Time PCR Workflow Flier
[ Add to Cart (Free) ]
6777
Protocol for Single Cell Gene Expression Profiling by Multiplex RT-qPCR
6816
Protocol for Multiplex RT-qPCR Analysis Using PrimePCR™ Assays, Ver A
6833
High Throughput Single Cell Gene Expression Profiling by Multiplex qPCR, Ver A