iScript™ Reverse Transcription Supermix for RT-qPCR

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iScript Reverse Transcription Supermix for RT-qPCR

Overview

Provides superior data reproducibility with reduced pipetting variability using a convenient master mix format containing all the essential components (except RNA). This simple, one-component reverse transcription kit uses a short 26-minute protocol.
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Description

The iScript reverse transcription supermix for RT-qPCR is a simple, fast, and sensitive first-strand cDNA synthesis kit for gene expression analysis using real-time qPCR. In one tube, the preblended 5x supermix contains all the necessary components except RNA template for reverse transcription.

  • Increase reproducibility of reverse transcription and reduce errors during setup — 1-tube format for simple and fast setup and reduced pipetting variability
  • Detect low-level target genes and conserve RNA during gene expression analysis — broad linear dynamic range of total input RNA (1 µg–1 pg) with a highly efficient RNase H+ MMLV reverse transcriptase
  • Use with dilute RNA samples — 5x concentration of supermix allows for greater volume of RNA sample in a 20 µl cDNA synthesis reaction
  • Eliminate freeze/thaw cycles — liquid format at –20ºC shortens overall processing time and maximizes stability
  • Validate purity of input RNA and gene expression results — optional no-RT controls for detecting genomic DNA contamination
  • Obtain accurate results — potent blend of RNase inhibitor prevents RNA degradation during reaction setup and reverse transcription
  • Increase primer design flexibility and prevent 5' and 3' bias — optimum blend of oligo(dT) and random primers in the 5x supermix for complete RNA coverage
  • Complete cDNA synthesis and qPCR the same day — fast 26 minute cDNA synthesis protocol

Specifications

Storage at –20ºC Guaranteed for 12 months in a constant temperature freezer (this reagent will not freeze at –20ºC; multiple freeze thaws not recommended)

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iScript™ Reverse Transcription Supermix, 25 x 20 µl rxns, 100 µl

1708840
25 x 20 µl reactions, 100 µl 5x supermix, contains reverse transcriptase, RNase inhibitor, dNTPs, primers, MgCl2, stabilizers; 50 reactions of no-RT control supermix

1708840 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/170-8840_1284066528.xml 1708840 09/09/10 02:05 PM en_US true internet/product_SKU 2020-07-10 17:39:25 /default/main/bio-rad/STAGING AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/170-8840_1284066528.xml /default/main/bio-rad/WORKAREA/default jcrucyr7 2017-11-14 07:07:35 true jcrucyr7 templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/170-8840_1284066528.xml LSR N /default/main/bio-rad en 12/31/37 11:59 PM 1556988417474 2020-07-10 17:39:25 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/170-8840_1284066528.xml 1708840-iscript-reverse-transcription-supermix-25-x-20-ul-rxns-100-ul 1 1708840 iScript Reverse Transcription Supermix for RT-qPCR iScript™ Reverse Transcription Supermix, 25 x 20 µl rxns, 100 µl 25 x 20 &micro;l reactions, 100 &micro;l 5x supermix, contains reverse transcriptase, RNase inhibitor, dNTPs, primers, MgCl<sub>2</sub>, stabilizers; 50 reactions of no-RT control supermix /webroot/web/images/lsr/products/amplification_pcr/sku_thumb/global/170-8840_tn.jpg /webroot/web/images/lsr/products/amplification_pcr/sku_view/global/170-8840_view.jpg ,VN Rick Abruzzo iScript&trade; Reverse Transcription Supermix for RT-qPCR 25 x 20 &micro;l reactions, 100 &micro;l 5x supermix contains reverse transcriptase, RNase inhibitor, dNTPs, primers, MgCl<sub>2</sub>, stabilizers; 50 reactions of no-RT control supermix 170-8840, 1708840, iscript, reverse, transcription, supermix, rt, qpcr, super, master, mix, mastermix, cdna, synthesis, real-time, real, time, pcr, amplification, transcriptase 09/09/10 02:05 PM 12/31/37 11:59 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSR /LSR/Amplification---PCR/PCR-Reagents N 0 <p>Choose iScript Reverse Transcription Supermix for RT-qPCR for fast synthesis of first-strand cDNA for direct use in real-time qPCR. This single-tube supermix contains all the components for reverse transcription except RNA template.</p> <h2 class="descHeader">Key Features and Benefits</h2> <ul> <li>Combined oligo(dT) and random primers provide unbiased representation of 5' and 3' regions</li> <li>Contains RNase H+ MMLV reverse transcriptase and has a linear range of 1 &micro;g&ndash;1 pg total input RNA</li> <li>Amplify cDNA for genes with low expression levels</li> <li>Fast 26-minute 1-tube cDNA synthesis protocol</li> <li>50 reactions of no-RT controls for optional detection of gDNA amplicon contamination</li> </ul> <h2 class="descHeader">Packaging Options</h2> <table class="pd_table pd_gridlines" border="0"> <tbody> <tr> <td valign="top"><strong>Kit Contents</strong></td> <td valign="top"><strong>Supermix Volume, &micro;l</strong></td> <td valign="top"><strong>Catalog Number</strong></td> </tr> <tr class="pd_colorbackground"> <td valign="top">25 x 20 &micro;l reactions</td> <td valign="top">100 (1 x 100 &micro;l)</td> <td valign="top">1708840</td> </tr> <tr> <td valign="top">100 x 20 &micro;l reactions</td> <td valign="top">&nbsp; 400 (4 x 100 &micro;l)</td> <td valign="top"><a href="/en-us/sku/1708841-iscript-reverse-transcription-supermix-100-x-20-ul-rxns-400-ul">1708841</a></td> </tr> <tr class="pd_colorbackground"> <td>500 x 20 &micro;l reactions</td> <td>2000 (20 x 100 &micro;l)</td> <td><a href="/en-us/sku/1708841bun-iscript-reverse-transcription-supermix-500-x-20-ul-rxns-2-ml?ID=1708841BUN">1708841BUN</a></td> </tr> </tbody> </table> <br /> <h2 class="descHeader">Related Products</h2> <ul> <li><a href="/en-us/category/cdna-synthesis-kits">cDNA Synthesis Kits</a></li> <li><a href="/en-us/category/supermixes-for-pcr-real-time-pcr">Real-Time PCR Supermixes and Kits</a></li> <li><a href="/en-us/product/primepcr-pcr-primers-assays">PrimePCR&trade; PCR Primers &amp; Assays</a></li> </ul> <h2 class="descHeader">Related Categories</h2> <ul> <li><a href="/en-us/category/pcr-reagents-qpcr-reagents">PCR and qPCR Reagents</a></li> <li><a href="/en-us/category/real-time-pcr-detection-systems">Real-Time PCR Detection Systems</a></li> <li><a href="/en-us/category/pcr-plastic-consumables">PCR Plastic Consumables</a></li> <li><a href="/en-us/category/pcr-instrument-validation-tools-services">Instrument Validation Tools and Services</a></li> </ul> <h2 class="descHeader">Supporting Documents</h2> <ul> <li>iScript Reverse Transcription Supermix <a href="/webroot/web/pdf/lsr/literature/10020178.pdf" target="_blank" rel="noopener noreferrer">Product Insert</a></li> <li>Amplification Plastics and Reagents <a href="/webroot/web/pdf/lsr/literature/10022527.pdf" target="_blank" rel="noopener noreferrer">Selection Guide</a></li> </ul> <h2 class="descHeader">Online Resources</h2> <ul> <li><a href="/en-us/applications-technologies/qpcr-real-time-pcr">qPCR/Real-Time PCR</a></li> <li><a href="/en-us/applications-technologies/genomics">Genomics</a></li> </ul> /webroot/web/html/lsr/products/amplification_pcr/sku_view/description/global iscript--reverse-transcription-supermix-for-rt-qpcr-170-8840-desc.html /webroot/web/html/lsr/products/amplification_pcr/sku_view/description/global/iscript--reverse-transcription-supermix-for-rt-qpcr-170-8840-desc.html &nbsp; 0 false $180.00 false $ null true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1708840&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1708840&CartQuote=QT&quantity= 1445 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1708840&AddToDefaultWishList=true&quantity= 32121600 180.00 Product detail page 1708840
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iScript™ Reverse Transcription Supermix, 100 x 20 µl rxns, 400 µl

1708841
100 x 20 µl reactions, 4 x 100 µl 5x supermix, contains reverse transcriptase, RNase inhibitor, dNTPs, primers, MgCl2, stabilizers; 50 reactions of no-RT control supermix

1708841 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/170-8841_1284066788.xml 1708841 09/09/10 02:10 PM en_US true internet/product_SKU 2020-07-10 17:39:25 /default/main/bio-rad/STAGING AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/170-8841_1284066788.xml /default/main/bio-rad/WORKAREA/default jcihuc4t 2017-11-14 07:07:35 true jcihuc4t templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/170-8841_1284066788.xml LSR N /default/main/bio-rad en 12/31/37 11:59 PM 1556988417516 2020-07-10 17:39:25 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/170-8841_1284066788.xml 1708841-iscript-reverse-transcription-supermix-100-x-20-ul-rxns-400-ul 1 1708841 iScript Reverse Transcription Supermix for RT-qPCR iScript™ Reverse Transcription Supermix, 100 x 20 µl rxns, 400 µl 100 x 20 &micro;l reactions, 4 x 100 &micro;l 5x supermix, contains reverse transcriptase, RNase inhibitor, dNTPs, primers, MgCl<sub>2</sub>, stabilizers; 50 reactions of no-RT control supermix /webroot/web/images/lsr/products/amplification_pcr/sku_thumb/global/170-8841_tn.jpg /webroot/web/images/lsr/products/amplification_pcr/sku_view/global/170-8841_view.jpg ,VN Rick Abruzzo iScript&trade; Reverse Transcription Supermix for RT-qPCR 100 x 20 &micro;l reactions, 4 x 100 &micro;l 5x supermix contains reverse transcriptase, RNase inhibitor, dNTPs, primers, MgCl<sub>2</sub>, stabilizers; 50 reactions of no-RT control supermix 170-8841, 1708841, iscript, reverse, transcription, supermix, rt, qpcr, super, master, mix, mastermix, cdna, synthesis, real-time, real, time, pcr, amplification, transcriptase, rt-pcr 09/09/10 02:10 PM 12/31/37 11:59 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSR /LSR/Amplification---PCR/PCR-Reagents N 0 <p>Choose iScript Reverse Transcription Supermix for RT-qPCR for fast synthesis of first-strand cDNA for direct use in real-time qPCR. This single-tube supermix contains all the components for reverse transcription except RNA template.</p> <h2 class="descHeader">Key Features and Benefits</h2> <ul> <li>Combined oligo(dT) and random primers provide unbiased representation of 5' and 3' regions</li> <li>Contains RNase H+ MMLV reverse transcriptase and has a linear range of 1 &micro;g&ndash;1 pg total input RNA</li> <li>Amplify cDNA for genes with low expression levels</li> <li>Fast 26-minute 1-tube cDNA synthesis protocol</li> <li>50 reactions of no-RT controls for optional detection of gDNA amplicon contamination</li> </ul> <h2 class="descHeader">Packaging Options</h2> <table class="pd_table pd_gridlines" border="0"> <tbody> <tr> <td valign="top"><strong>Kit Contents</strong></td> <td valign="top"><strong>Supermix Volume, &micro;l</strong></td> <td valign="top"><strong>Catalog Number</strong></td> </tr> <tr class="pd_colorbackground"> <td>25 x 20 &micro;l reactions</td> <td valign="top">&nbsp; 100 (1 x 100 &micro;l)</td> <td valign="top"><a href="/en-us/sku/1708840-iscript-reverse-transcription-supermix-25-x-20-ul-rxns-100-ul">1708840</a></td> </tr> <tr> <td>100 x 20 &micro;l reactions</td> <td valign="top">&nbsp; 400 (4 x 100 &micro;l)</td> <td valign="top">1708841</td> </tr> <tr class="pd_colorbackground"> <td>500 x 20 &micro;l reactions</td> <td>2000 (20 x 100 &micro;l)</td> <td><a href="/en-us/sku/1708841bun-iscript-reverse-transcription-supermix-500-x-20-ul-rxns-2-ml?ID=1708841BUN">1708841BUN</a></td> </tr> </tbody> </table> <br /> <p>See the <a href="/en-us/product/iscript-reverse-transcription-supermix-for-rt-qpcr">iScript Reverse Transcription Supermix</a> product page for more information and documentation.</p> <h2 class="descHeader">Related Products</h2> <ul> <li><a href="/en-us/category/cdna-synthesis-kits">cDNA Synthesis Kits</a></li> <li><a href="/en-us/category/supermixes-for-pcr-real-time-pcr">Real-Time PCR Supermixes and Kits</a></li> <li><a href="/en-us/product/primepcr-pcr-primers-assays">PrimePCR&trade; PCR Primers &amp; Assays</a></li> </ul> <h2 class="descHeader">Related Categories</h2> <ul> <li><a href="/en-us/category/pcr-reagents-qpcr-reagents">PCR and qPCR Reagents</a></li> <li><a href="/en-us/category/real-time-pcr-detection-systems">Real-Time PCR Detection Systems</a></li> <li><a href="/en-us/category/pcr-plastic-consumables">PCR Plastic Consumables</a></li> <li><a href="/en-us/category/pcr-instrument-validation-tools-services">Instrument Validation Tools and Services</a></li> </ul> <h2 class="descHeader">Supporting Documents</h2> <ul> <li>iScript Reverse Transcription Supermix <a href="/webroot/web/pdf/lsr/literature/10020178.pdf" target="_blank" rel="noopener noreferrer">Product Insert</a></li> <li>Amplification Plastics and Reagents <a href="/webroot/web/pdf/lsr/literature/10022527.pdf" target="_blank" rel="noopener noreferrer">Selection Guide</a></li> </ul> <h2 class="descHeader">Online Resources</h2> <ul> <li><a href="/en-us/applications-technologies/qpcr-real-time-pcr">qPCR/Real-Time PCR</a></li> <li><a href="/en-us/applications-technologies/genomics">Genomics</a></li> </ul> /webroot/web/html/lsr/products/amplification_pcr/sku_view/description/global iscript-reverse-transcription-supermix-for-rt-qpcr-170-8841-desc.html /webroot/web/html/lsr/products/amplification_pcr/sku_view/description/global/iscript-reverse-transcription-supermix-for-rt-qpcr-170-8841-desc.html &nbsp; 0 false $546.00 false $ null true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1708841&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1708841&CartQuote=QT&quantity= 1445 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1708841&AddToDefaultWishList=true&quantity= 31141500 546.00 Product detail page 1708841
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iScript™ Reverse Transcription Supermix, 500 x 20 µl rxns, 2 ml

1708841BUN
500 x 20 µl reactions, 20 x 100 µl 5x supermix, contains reverse transcriptase, RNase inhibitor, dNTPs, primers, MgCl2, stabilizers; 4 x 50 reactions of no-RT control supermix

1708841BUN templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/1708841BUN_1526666954.xml 1708841BUN 05/18/18 05:48 PM en_US true internet/product_SKU 2019-05-04 16:18:45 /default/main/bio-rad/STAGING AR,BO,BR,CA,CL,CO,CR,DO,EC,SV,GU,GT,HT,HN,JM,MX,NI,PA,PY,PE,PR,TT,US,UY,VE,AU,BD,BN,KH,CN,HK,IN,ID,JP,KR,MO,MY,FM,NP,NZ,PW,PG,PH,SG,SB,LK,TW,TH,TO,VU,AL,DZ,AI,AM,AT,AZ,BH,BE,BA,BW,BG,BF,CM,HR,CY,CZ,DK,EG,ER,EE,ET,FO,FI,FR,GF,PF,GA,GE,DE,GH,GR,GP,HU,IS,IE,IL,IT,JO,KZ,KE,XK,KW,LV,LB,LI,LT,LU,MK,MG,ML,MT,MQ,MU,MD,MS,MA,NL,NG,NO,OM,PK,PS,PL,PT,QA,RO,RU,ST,SA,SN,RS,SK,SI,ZA,ES,SE,CH,TZ,TG,TN,TR,UG,UA,AE,UK,UZ,VA,EH,YE,VN templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/1708841BUN_1526666954.xml /default/main/bio-rad/WORKAREA/default jh81v6e0 2018-05-18 18:09:14 true jh81v6e0 templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/1708841BUN_1526666954.xml LSR N /default/main/bio-rad en 05/18/28 07:53 AM 1555974590125 2019-05-04 16:18:45 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/1708841BUN_1526666954.xml 1708841bun-iscript-reverse-transcription-supermix-500-x-20-ul-rxns-2-ml 1 1708841BUN iScript Reverse Transcription Smx 500 iScript™ Reverse Transcription Supermix, 500 x 20 µl rxns, 2 ml 500 x 20 µl reactions, 20 x 100 µl 5x supermix, contains reverse transcriptase, RNase inhibitor, dNTPs, primers, MgCl2, stabilizers; 4 x 50 reactions of no-RT control supermix /webroot/web/images/lsr/products/amplification_pcr/sku_thumb/global/1708841bun_tn.jpg /webroot/web/images/lsr/products/amplification_pcr/sku_view/global/170-8841bun_view.jpg iScript™ Reverse Transcription Supermix, 500 x 20 µl rxns, 2 ml preview ,VN Tommy A iScript™ Reverse Transcription Supermix, 500 x 20 µl rxns, 2 ml iScript, Supermix, RT, Reverse transcription 05/18/18 05:48 PM 05/18/28 07:53 AM AR,BO,BR,CA,CL,CO,CR,DO,EC,SV,GU,GT,HT,HN,JM,MX,NI,PA,PY,PE,PR,TT,US,UY,VE,AU,BD,BN,KH,CN,HK,IN,ID,JP,KR,MO,MY,FM,NP,NZ,PW,PG,PH,SG,SB,LK,TW,TH,TO,VU,AL,DZ,AI,AM,AT,AZ,BH,BE,BA,BW,BG,BF,CM,HR,CY,CZ,DK,EG,ER,EE,ET,FO,FI,FR,GF,PF,GA,GE,DE,GH,GR,GP,HU,IS,IE,IL,IT,JO,KZ,KE,XK,KW,LV,LB,LI,LT,LU,MK,MG,ML,MT,MQ,MU,MD,MS,MA,NL,NG,NO,OM,PK,PS,PL,PT,QA,RO,RU,ST,SA,SN,RS,SK,SI,ZA,ES,SE,CH,TZ,TG,TN,TR,UG,UA,AE,UK,UZ,VA,EH,YE,VN LSR /LSR/Amplification---PCR/PCR-Reagents N 0 <p>Choose iScript Reverse Transcription Supermix for RT-qPCR for fast synthesis of first-strand cDNA for direct use in real-time qPCR. This single-tube supermix contains all the components for reverse transcription except RNA template.</p> <h2 class="descHeader">Key Features and Benefits</h2> <ul> <li>Combined oligo(dT) and random primers provide unbiased representation of 5' and 3' regions</li> <li>Contains RNase H+ MMLV reverse transcriptase and has a linear range of 1 &micro;g&ndash;1 pg total input RNA</li> <li>Amplify cDNA for genes with low expression levels</li> <li>Fast 26-minute 1-tube cDNA synthesis protocol</li> <li>50 reactions of no-RT controls for optional detection of gDNA amplicon contamination</li> </ul> <h2 class="descHeader">Packaging Options</h2> <table class="pd_table pd_gridlines" border="0"> <tbody> <tr> <td align="left"><strong>Kit Size</strong></td> <td valign="top"><strong>Supermix Volume, &micro;l</strong></td> <td valign="top"><strong>Catalog Number</strong></td> </tr> <tr class="pd_colorbackground"> <td>25 x 20 &micro;l reactions</td> <td>100 (1 x 100 &micro;l)</td> <td><a href="/en-us/sku/1708840-iscript-reverse-transcription-supermix-25-x-20-ul-rxns-100-ul">1708840</a></td> </tr> <tr> <td>100 x 20 &micro;l reactions</td> <td>400 (4 x 100 &micro;l)</td> <td><a href="/en-us/sku/1708841-iscript-reverse-transcription-supermix-100-x-20-ul-rxns-400-ul?ID=1708841">1708841</a></td> </tr> <tr class="pd_colorbackground"> <td>500 x 20 &micro;l reactions</td> <td>2000 (20 x 100 &micro;l)</td> <td>1708841BUN</td> </tr> </tbody> </table> <br /> <p>See the <a href="/en-us/product/iscript-reverse-transcription-supermix-for-rt-qpcr">iScript Reverse Transcription Supermix</a> product page for more information and documentation.</p> <h2 class="descHeader">Related Products</h2> <ul> <li><a href="/en-us/category/cdna-synthesis-kits">cDNA Synthesis Kits</a></li> <li><a href="/en-us/category/supermixes-for-pcr-real-time-pcr">Real-Time PCR Supermixes and Kits</a></li> <li><a href="/en-us/product/primepcr-pcr-primers-assays">PrimePCR&trade; PCR Primers &amp; Assays</a></li> </ul> <h2 class="descHeader">Related Categories</h2> <ul> <li><a href="/en-us/category/pcr-reagents-qpcr-reagents">PCR and qPCR Reagents</a></li> <li><a href="/en-us/category/real-time-pcr-detection-systems">Real-Time PCR Detection Systems</a></li> <li><a href="/en-us/category/pcr-plastic-consumables">PCR Plastic Consumables</a></li> <li><a href="/en-us/category/pcr-instrument-validation-tools-services">Instrument Validation Tools and Services</a></li> </ul> <h2 class="descHeader">Supporting Documents</h2> <ul> <li>iScript Reverse Transcription Supermix <a href="/webroot/web/pdf/lsr/literature/10020178.pdf" target="_blank" class="no-target-style" rel="noopener noreferrer">Product Insert</a></li> <li>Amplification Plastics and Reagents <a href="/webroot/web/pdf/lsr/literature/10022527.pdf" target="_blank" class="no-target-style" rel="noopener noreferrer">Selection Guide</a></li> </ul> <h2 class="descHeader">Online Resources</h2> <ul> <li><a href="/en-us/applications-technologies/qpcr-real-time-pcr">qPCR/Real-Time PCR</a></li> <li><a href="/en-us/applications-technologies/genomics">Genomics</a></li> </ul> 1708841bun.html /1708841bun.html 1 0 false $2,494.00 false $ null true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1708841BUN&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1708841BUN&CartQuote=QT&quantity= 1445 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1708841BUN&AddToDefaultWishList=true&quantity= 41106300 2494.00 Product detail page 1708841BUN
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Number Description Options
10020178 iScript™ Reverse Transcription Supermix for RT-qPCR Product Insert, Ver C Click to download
6031 iScript™ Reverse Transcription Supermix for RT-qPCR Product Information Sheet, Rev B Click to download
6746 iScript Reverse Transcription Kits Flier Click to download [ Add to Cart (Free) ]
6252 Reagent Comparison Guide for Real-Time PCR Brochure, Rev C Click to download [ Add to Cart (Free) ]
5990 Gene Expression Analysis Solutions Brochure Click to download
6090 Amplification Reagents and Plastics Brochure Click to download [ Add to Cart (Free) ]
6763 Gene Expression Real-Time PCR Workflow Flier Click to download

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