Overview
25 ml (5 x 5 ml vials), 2x qPCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, stabilizers, ROX normalization dyes, for 2,500 x 20 µl reactions
Safety Data Sheet
Description
Description
SsoAdvanced Universal Probes Supermix is a high-performance real-time PCR supermix based on Bio-Rad's patented* Sso7d fusion protein technology. This supermix is formulated for a wide range of real-time PCR applications and for use with all ROX dependent or independent real-time PCR systems. The dsDNA binding protein, Sso7d, stabilizes the polymerase-template complex, providing superior inhibitor tolerance, increased processivity, specificity, and greater speed without affecting PCR sensitivity, efficiency, or reproducibility.
SsoAdvanced universal probes supermix lets you:
- Carry out high-performance singleplex and duplex reactions — the Sso7d fusion polymerase and advanced formulation enable robust performance in singleplex or duplex real-time PCR reactions, providing the highest data precision and allowing cost and time savings when combining 2 assays in a single well
- Use any real-time PCR system — the universal reference dye in this supermix enables ROX normalization of quantitative PCR (qPCR) data regardless of the ROX level requirements of the qPCR system
- Achieve superior real-time PCR results under various conditions — our robust formulation delivers consistent performance in fast cycling across a broad range of reaction conditions, primer concentrations, and temperature ranges
- Increase your qPCR sensitivity and efficiency of detection from compromised samples — the Sso7d fusion polymerase has increased resistance to a wide variety of PCR inhibitors, which helps provide better sensitivity and overall performance
- Decrease time to results without compromising qPCR data quality — the Sso7d fusion polymerase and optimized buffer together provide rapid polymerization kinetics and instant polymerase activation
Instrument Compatibility
SsoAdvanced Universal Probes Supermix is compatible with Bio-Rad Real-Time PCR Detection Systems and commercially available systems listed below:
| Systems | |
| Applied Biosystems |
|
| Stratagene |
|
| Eppendorf |
|
| QIAGEN |
|
| Roche |
|
| BioFire |
|
* BSA must be added according to instrument specifications
Applications and Uses of SsoAdvanced Universal Probes Supermix
- qPCR/real-time PCR
- Gene expression analysis
- Absolute quantification
- Genotyping (allelic discrimination)
- Mutation detection
- Pathogen detection
- Viral detection (load)
- Characterization of genetically modified organisms (GMO)
- Genetic profiling
Packaging Options
| Number of 20 μl Reactions | Total Volume | Number of Vials | Catalog # |
| 200 | 2 ml | 2 x 1 ml | 1725280 |
| 500 | 5 ml | 5 x 1 ml | 1725281 |
| 1,000 | 10 ml | 10 x 1 ml | 1725282 |
| 2,500 | 25 ml | 5 x 5 ml | 1725284 |
| 5,000 | 50 ml | 10 x 5 ml | 1725285 |
Related Products
We have developed SsoAdvanced Universal SYBR® Green Supermix, which is available in several formats (200–5,000 reactions).
Bio-Rad offers a complete workflow solution for real-time PCR, including:
More Information
To ensure that your qPCR reactions are reproducible and reliable, follow our step-by-step design and optimization protocols. We also provide insights into analysis of qPCR data and troubleshooting reactions.
* U.S. patent 7,560,260