Precision Plus Protein™ Unstained Standards

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Overview

Unstained Standards

Precision Plus Protein unstained standards on a gel. These standards have been engineered to contain the unique Strep-tag affinity peptide, which allows detection and molecular weight determination on western blots.

Detection Using Immun-Star HRP Kit

Western blot detection of Green Fluorescent Protein (GFP) and unstained Precision Plus Protein standards using the Immun-Star horseradish peroxide (HRP) chemiluminescent detection kit. A gel was run with 0.5 µl of standards (lane 1) and a dilution series of E. coli lysate containing GFP (lanes 2–8), and was transferred to a PVDF membrane. The blot was probed with a primary antibody specific for GFP, then incubated simultaneously with goat anti-mouse-HRP and StrepTactin-HRP. After incubating in the Immun-Star HRP detection solution for 2 min, the blot was exposed to film for 10 min.

Detection Using Immun-Blot AP Kit

Western blot detection of Green Fluorescent Protein (GFP) and unstained Precision Plus Protein standards using the Immun-Blot alkaline phosphatase (AP) colorimetric detection Kit. Maximum sensitivity achievable with Immun-Blot AP is 100 pg. A gel run with 4 µl of standards (lane 1) and a dilution series of E. coli lysate containing overexpressed GFP (lanes 2–10) was transferred to a PVDF membrane. The blot was probed with a primary antibody specific for GFP, then incubated with StrepTactin-AP and a secondary antibody conjugated to AP. The blot was developed using the Immun-Blot AP kit.

Precision Plus Protein™ standards for protein electrophoresis offer unsurpassed band sharpness, accurate MW determination, lot-to-lot consistency, and excellent blotting results. These standards contain ten highly purified recombinant proteins with molecular masses from 10 to 250 kD. These unstained standards include three high-intensity reference bands (25, 50, and 75 kD).

Precision Plus Protein unstained standards have been engineered to contain the unique Strep-tag affinity peptide, which allows detection and molecular weight determination on western blots. These standards offer absolute molecular weight accuracy confirmed by mass spectrometry, with a known amount of protein in each band to allow approximation of protein concentration in your sample. These standards are compatible with Laemmli and neutral pH buffer systems and can be used for accurate molecular weight determination on gels and blots.

Precision Plus Protein unstained standards are also an excellent choice for use with stain-free gels and the
Gel Doc™ EZ imaging system.

All Precision Plus Protein standards generate a standard curve with r2 values >0.99, demonstrating a strong linear relationship between the proteins' log molecular weight and relative migration distance on a gel.

MW size range  10–250 kD 
Stain type Unstained
Volume 1 ml
Number of applications 100
Loading buffer 30% (w/v) glycerol, 2% SDS,
62.5 mM Tris, pH 6.8, 50 mM DTT,
5 mM EDTA, 0.02% NaN3,
0.01% bromophenol blue
Storage   Store at –20ºC until expiration date (24 months from date of manufacture)
Precision Plus Protein™ Unstained Standards
Precision Plus Protein™ Unstained Standards

161-0363
1 ml, unstained mixture of 10 Strep-tagged recombinant proteins (10–250 kD), including three reference bands (25, 50, 75 kD), 100 applications

List Price:   $91.00
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Precision Plus Protein™ Unstained Standards, Value Pack

161-0396
5 ml (5 x 1 ml), unstained mixture of 10 Strep-tagged recombinant proteins (10–250 kD), including 3 reference bands (25, 50, 75 kD), 500 applications

List Price:   $390.00
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Precision Plus Protein™ Standard Plugs
Precision Plus Protein Standard Plugs

161-0378
Pkg of 24, 1 mm thick agarose plugs containing 10 Strep-tagged recombinant proteins (10–250 kD), including three reference bands

List Price:   $115.00
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Precision Protein™ StrepTactin-HRP Conjugate
Precision Protein™ StrepTactin-HRP Conjugate

161-0380
300 μl, StrepTactin and horseradish peroxidase (HRP) conjugate used for detection of Precision Plus Protein unstained standards, 150 applications

List Price:   $86.00
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Precision Protein™ StrepTactin-HRP Conjugate
Precision Protein™ StrepTactin-HRP Conjugate

161-0381
125 μl, StrepTactin and horseradish peroxidase (HRP) conjugate used for detection of Precision Plus Protein unstained standards, 50 applications

List Price:   $31.00
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Precision Protein™ StrepTactin-AP Conjugate
Precision Protein™ StrepTactin-AP Conjugate

161-0382
300 μl, StrepTactin and alkaline phosphatase (AP) conjugate used for detection of Precision Plus Protein unstained standards, 150 applications

List Price:   $86.00
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Number Description Options
6533 Introducing Bio-Rad’s TGX and TGX Stain-Free FastCast Acrylamide Kits, Rev A Click to download
2895 Protein Blotting Guide, Interactive PDF, Rev B Click to download
6040 Electrophoresis Guide, Interactive PDF, Rev B Click to download
2032 Western Blotting Detection Reagents Brochure, Rev F Click to download [ Add to Cart (Free) ]
3036 Precision Plus Protein Plugs Promotional Card, Rev C Click to download
3117 Blotting Starter Kits, Rev B Click to download
2414 The Little Book of Standards, Rev D Click to download [ Add to Cart (Free) ]
2847 Strep-tag Technology for Molecular Weight (MW) Determinations on Blots Using Precision Plus Protein Standards, Rev B Click to download
3118 Protein Standards for Electrophoresis and Blotting Brochure, Rev D Click to download [ Add to Cart (Free) ]
3133 Molecular Weight Determination by SDS-PAGE, Rev B Click to download
2998 Protein Standards Application Guide, Rev G Click to download [ Add to Cart (Free) ]
3093 Precision Plus Protein Standards Competitive Comparison Card, Rev B Click to download
3144 Using Precision Plus Protein Standards to Determine Molecular Weight, Rev B Click to download
5561 Precision Plus Protein WesternC Standards, Rev E Click to download [ Add to Cart (Free) ]
6148 Transfer of High Molecular Weight Proteins to Membranes: A Comparison of Transfer Efficiency Between Blotting Systems, Rev B Click to download [ Add to Cart (Free) ]
4110000 Instruction Manual, Precision StrepTactin HRP-Conjugate and AP-Conjugate, Rev D Click to download
4117684 Instruction Manual, Precision Plus Protein Standard Plugs, Unstained, Rev C Click to download
4110023 Instruction Manual, Unstained Precision Plus Protein Standards, Rev B Click to download