Bio-Plex Pro™ Rat Cytokine, Chemokine, and Growth Factor Assays

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Bio-Plex Pro Rat Cytokine, Chemokine, and Growth Factor Assays

Overview

Available Bio-Plex Pro™ Rat Cytokine Assays


24-Plex
(171-K1001M)
12-Plex
Th1/Th2 Panel
(171-K1002M)
TGF-β 3-Plex
(171-W4001M)
Singleplex Sets
Bead Regions
EPO
    14
G-CSF
 
 
54
GM-CSF
  37
GRO/KC
    57
IFN-γ
  34
IL-1α
  21
IL-1β
  28
IL-2
  22
IL-4
  33
IL-5
  52
IL-6
  56
IL-7
    38
IL-10
  19
IL-12p70
  78
IL-13
  15
IL-17A
    72
IL-18*
      20
M-CSF
    26
MCP-1
    51
MIP-1α
    77
MIP-3α
    36
RANTES
    55
TNF-α
  43
VEGF
    47
IL-12p40**       76
MIP-2***       27
TGF-β1    
13
TGF-β2    
72
TGF-β3    
66


* IL-18 is not available as a singleplex assay but only as part of an assay panel.

** IL-12p40 cannot be multiplexed with IL-12p70.

*** MIP-2 cannot be multiplexed with GRO/KC.

Bio-Plex Pro™ Rat Cytokine Assay Specifications

Representative assay working ranges, assay sensitivity, and precision. The LLOQ, ULOQ, LOD, and inter-assay precision %CV are mean data determined from three independent multiplex assays in a serum-based matrix. Intra-assay %CV is derived from one representative assay. LLOQ and ULOQ are defined as the boundary standard curve points in which the performance specifications of individual standard points were met for <20% intra-assay CV, <30% inter-assay CV, and recovery range of 80–120%. Data were generated using the magnetic workflow with the Bio-Plex Pro II wash station.

 

Assay Working Ranges, pg/ml
Assay Sensitivity, pg/ml
Assay Precision
Targets
LLOQ
ULOQ
LOD
Intra-assay %CV Inter-assay %CV
Singleplex Assays
IL-12p40 2 2,015 0.4 3 4
MIP-2 0.3 1,085 0.2 4 5
 
Multiplex Assays
EPO 32 32,258 8 4 1
G-CSF 1 2,911 0.2 2 1
GM-CSF 6 31,519 0.6 4 1
GRO-KC 2 1,521 0.6 4 2
IFN-gamma 4 23,955 1 5 3
IL-1alpha 2 24,649 1 3 3
IL-1beta 6 31,666 2 4 2
IL-2 8 7,981 3 4 3
IL-4 1 16,209 1 6 3
IL-5 6 2,007 6 4 0.4
IL-6 13 30,766 10 4 3
IL-7 2 32,472 0.4 4 3
IL-10 4 34,385 5 4 6
IL-12p70 4 15,481 0.7 4 3
IL-13 2 15,751 0.9 4 2
IL-17 1 3,079 0.1 3 2
IL-18 4 31,652 4 4 5
M-CSF 2 23,264 0.4 2 3
MIP-1alpha 25 4,325 12 4 7
MIP-3alpha 1 3,032 0.7 4 2
RANTES 8 7,950 3 5 2
TNF-alpha 16 8,207 3 5 7
VEGF 0 3,963 0.3 3 5

Assay Performance Definitions

Assay working range — the range of concentrations within which the assay is precise and accurate. Boundaries of the assay working range are the lower limit of quantitation (LLOQ) and the upper limit of quantitation (ULOQ).

Precision — the coefficient of variation (%CV) at concentrations within the assay working range

Accuracy — ratio of the observed concentration versus the expected concentration of a known amount of spiked analyte within the assay working range

Sensitivity (Limit of Detection, LOD) — the concentration of analyte for which the fluorescence intensity signal is two-fold of the standard deviation above the background signal

TGF-β Assay Specifications Overlay
Incubation time
3-Plex Panel
(171-W4001)
Sample volume
12.5 µl
Accuracy (recovery range)
80–100%
Precision
   Intra-assay %CV
   Inter-assay %CV

   ≤10%
   ≤15%


Representative assay working ranges, assay sensitivity, and precision. The LLOQ, ULOQ, LOD, and inter-assay precision %CV are mean data determined from three independent multiplex assays in serum-based matrix. Intra-assay %CV is derived from one representative assay. LLOQ and ULOQ are defined as the boundary standard curve points in which the performance specifications of individual standard points were met for 10% intra-assay CV, 15% inter-assay CV, and recovery range of 80–100%. Data were generated using the magnetic workflow with the Bio-Plex Pro II wash station.

TGF-β Assay Specifications
 
Assay Working Ranges, pg/ml
Assay Sensitivity, pg/ml
Assay Precision
Targets LLOQ ULOQ LOD Intra-assay %CV Inter-assay %CV
Premixed 3-Plex Assay
TGF-β1 1.69  27,616 3.9
4.5
4.9
TGF-β2
14.7 
30,080 1.9
6.3 
9.1
TGF-β3
2.8 
15,031 0.5
6.9 
8.2


Assay Performance Definitions
Assay working range – the range of concentrations within which the assay is precise and accurate. Boundaries of the assay working range are the lower limit of quantitation (LLOQ) and the upper limit of quantitation (ULOQ)

Precision – the coefficient of variation (%CV) at concentrations within the assay working range

Accuracy – ratio of the observed concentration versus the expected concentration of a known amount of spiked analyte within the assay working range

Sensitivity (Limit of Detection, LOD) – the concentration of analyte for which the fluorescence intensity signal is 2-fold of the standard deviation above the background signal.

Bio-Plex® Technology and Applications

Bio-Plex Pro™ rat cytokine, chemokine, and growth factor magnetic bead–based assays detect and measure levels of 29 analytes in diverse matrices such as serum, plasma, and tissue culture supernatants. Cytokines, chemokines, and growth factors are cell signaling proteins that mediate a wide range of physiological responses, including immunity, inflammation, and hematopoiesis. They are associated with a spectrum of diseases ranging from tumor growth, to infections, to Parkinson's disease.

Based on 6.5 µm magnetic beads, these assays are compatible with both vacuum and magnetic wash stations. Magnetic washing simplifies plate processing, provides increased throughput, and results in decreased variability and increased reproducibility.

Key Features

  • Magnetic bead–based for simplified plate processing
  • Developed to deliver accurate, reproducible measurements in multiple sample matrices
  • Requires as little as 12.5 µl of sample for both multiplex and singleplex assays
  • Available as all-in-one, space-saving complete kits
  • Includes premixed standards for generation of standard curves over a broad dynamic range for each target
  • Compatible with the new Bio-Plex Pro rat diabetes assays for an expanded biomarker profile

Flexible Ordering Options

  • Premixed panels — complete ready-to-use kits (24-plex, 12-plex, TGF-β 3-plex)
  • Singleplex sets — each set includes a vial of coupled magnetic beads and a vial of detection bodies; requires a reagent kit (#171-304070 for vacuum separation or #171-304070M for magnetic separation) and a vial of standards (#171-NZ0001 for rat cytokines or #171-X40001 for TGF-β assays) for a complete assay
  • Custom premixed assays — available as Bio-Plex® x-Plex™ assays built using the Bio-Plex Assay Builder
  • Custom assays (mixed at the bench) —  available as Bio-Plex Express assays built using the Bio-Plex Assay Builder

Instrument Compatibility

  • Bio-Plex 200 system
  • Bio-Plex 3D system
  • Bio-Plex MAGPIX system
  • Bio-Plex Pro wash station
  • Bio-Plex Pro II wash station

More Information

See how Bio-Plex instruments and assays benefit researchers studying heart disease, leukemia, melanoma, and other cancers.

Bio-Plex Pro Rat Metabolic 5-plex Panel
Bio-Plex Pro™ Rat Cytokine 24-plex Assay

171-K1001M
1 x 96-well, includes premixed coupled magnetic beads and detection antibodies, standards, buffers, diluents, streptavidin-PE, flat bottom plate, sealing tape for detecting rat cytokines EPO, G-CSF, GM-CSF, GRO/KC, IFN-γ, IL-1α, L-1β, IL-2, IL-4, IL-5, IL-6, IL-7, IL-10, IL-12p70, IL-13, IL-17A, IL-18, M-CSF, MCP-1, MIP-1α, MIP-3α, RANTES, TNF-α, VEGF , all in one kit all in one kit

List Price:   $3,408.00
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Bio-Plex Pro Rat Metabolic 23-plex Panel
Bio-Plex Pro Rat Cytokine 23-Plex Assay

L80-01V11S5
1 x 96-well, includes premixed coupled magnetic beads and detection antibodies, standards, assay buffer, wash buffer, detection antibody diluent, streptavidin-PE, filter and flat bottom plate, sealing tape, standard diluent, sample diluent for detecting 24 rat cytokines all in one kit

List Price:   $3,328.00
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Bio-Plex Pro™ Rat Cytokine Th1/Th2 Assay

171-K1002M
1 x 96-well, includes premixed coupled magnetic beads and detection antibodies, standards, assay buffer, wash buffer, detection antibody diluent, streptavidin-PE, filter and flat bottom plate, sealing tape, standard diluent, sample diluent for detecting 12 rat cytokines all in one kit

List Price:   $2,331.00
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Bio-Plex Pro™ TGF-β 3-plex Assay

171-W4001M
1 x 96-well, includes coupled magnetic beads, detection antibodies, standards, buffers, diluents, streptavidin-PE, filter plate, sealing tape, for the detection of TGF-β1, TGF-β2, TGF-β3 all in one kit

List Price:   $866.00
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Number Description Options
6335 Bio-Plex® Multiplex Immunoassays Analyte Guide, Interactive PDF, Rev D Click to download
5405 Bio-Plex Multiplex System Brochure, Rev D Click to download [ Add to Cart (Free) ]
6100 Bio-Plex Pro Rat Cytokine Assays Product Information Sheet, Rev B Click to download [ Add to Cart (Free) ]
10014905 Instruction Manual, Bio-Plex Pro Cytokine, Chemokine, and Growth Factors Assays, Rev D Click to download
10024985 Instruction Manual, Bio-Plex Pro Assays Quick Guide 4, Rev A Click to download
6054 Bio-Plex Pro TGF-beta Assays Product Information Sheet, Rev B Click to download [ Add to Cart (Free) ]
2993 Bio-Plex Glossary, Rev A Click to download
BR125FS Second Generation Multiplex Immunoassays Click to download
6457 The Bio-Plex Success Guide for Multiplex Immunoassays, Rev B Click to download
6458 Bio-Plex Success Guide/Assay Sample Prep, Rev B Click to download