Bio-Plex Pro™ TGF-β Assays

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Bio-Plex Pro™ TGF-β Assays

Overview

TGF-β Assay Specifications Overlay
Incubation time
3-Plex Panel
(171-W4001)
Sample volume
12.5 µl
Accuracy (recovery range)
80–100%
Precision
   Intra-assay %CV
   Inter-assay %CV

   ≤10%
   ≤15%


Representative assay working ranges, assay sensitivity, and precision. The LLOQ, ULOQ, LOD, and inter-assay precision %CV are mean data determined from three independent multiplex assays in serum-based matrix. Intra-assay %CV is derived from one representative assay. LLOQ and ULOQ are defined as the boundary standard curve points in which the performance specifications of individual standard points were met for 10% intra-assay CV, 15% inter-assay CV, and recovery range of 80–100%. Data were generated using the magnetic workflow with the Bio-Plex Pro II wash station.

TGF-β Assay Specifications
 
Assay Working Ranges, pg/ml
Assay Sensitivity, pg/ml
Assay Precision
Targets LLOQ ULOQ LOD Intra-assay %CV Inter-assay %CV
Premixed 3-Plex Assay
TGF-β1 1.69  27,616 3.9
4.5
4.9
TGF-β2
14.7 
30,080 1.9
6.3 
9.1
TGF-β3
2.8 
15,031 0.5
6.9 
8.2


Assay Performance Definitions
Assay working range – the range of concentrations within which the assay is precise and accurate. Boundaries of the assay working range are the lower limit of quantitation (LLOQ) and the upper limit of quantitation (ULOQ)

Precision – the coefficient of variation (%CV) at concentrations within the assay working range

Accuracy – ratio of the observed concentration versus the expected concentration of a known amount of spiked analyte within the assay working range

Sensitivity (Limit of Detection, LOD) – the concentration of analyte for which the fluorescence intensity signal is 2-fold of the standard deviation above the background signal.

Bio-Plex® Technology and Applications

Bio-Plex Pro™ TGF-β assays are magnetic bead–based multiplex assays designed to measure levels of TGF-β1, TGF-β2, and TGF-β3 in human, mouse, and rat models in diverse matrices like serum, plasma, urine, and human milk.

Based on 6.5 µm magnetic beads, these assays are compatible with both vacuum and magnetic wash stations. Magnetic washing simplifies plate processing, provides increased throughput, and results in decreased variability and increased reproducibility.

Key Features

  • Magnetic bead–based for simplified plate processing
  • Developed to deliver accurate, reproducible measurements in multiple sample matrices, including milk and urine
  • Requires as little as 12.5 µl of sample for both multiplex and singleplex assays
  • Available as an all-in-one, space-saving complete kit
  • Includes premixed standards for generation of standard curves over a broad dynamic range for each target

Flexible Ordering Options

  • Bio-Plex Pro TGF-β 3-plex immunoassay — a multiplex assay that detects TGF-β1, TGF-β2, and TGF-β3
  • Bio-Plex Pro TGF-β singleplex sets — each set includes a vial of coupled magnetic beads and a vial of detection bodies; requires a reagent kit (#171-304070 for vacuum separation or #171-304070M for magnetic separation) and a vial of standards (#171-X40001) for a complete assay

TGF-β assays are not available in 10 x 96-well formats. Reagent kits in the 10 x 96-well format cannot be used for TGF-β assays.

Instrument Compatibility

  • Bio-Plex® 200 system
  • Bio-Plex 3D system
  • Luminex MAGPIX system
  • Bio-Plex Pro wash station
  • Bio-Plex Pro II wash station

More Information

See how Bio-Plex instruments and assays benefit researchers studying heart disease, leukemia, melanoma, and other cancers.

Bio-Plex Pro™ TGF-β 3-plex Assay

171-W4001M
1 x 96-well, includes coupled magnetic beads, detection antibodies, standards, buffers, diluents, streptavidin-PE, filter plate, sealing tape, for the detection of TGF-β1, TGF-β2, TGF-β3 all in one kit

List Price:   $866.00
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Bio-Plex Pro™ TGF-β1 Set

171-V4001M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting TGF-β1, requires reagent kit (#171-304070 for vacuum separation or #171-304070M for magnetic separation) and a vial of standards (#171-X40001)

List Price:   $220.00
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Bio-Plex Pro™ TGF-β2 Set

171-V4002M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting TGF-β2, requires reagent kit (#171-304070 for vacuum separation or #171-304070M for magnetic separation) and a vial of standards (#171-X40001)

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Bio-Plex Pro™ TGF-β3 Set

171-V4003M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting TGF-β3, requires reagent kit (#171-304070 for vacuum separation or #171-304070M for magnetic separation) and a vial of standards (#171-X40001)

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Number Description Options
6127 Bio-Plex Pro™ TGF-β Assays for Simultaneous Profiling of TGF-β1, -β2, and -β3 in Human and Animal Models Click to download
6335 Bio-Plex® Multiplex Immunoassays Analyte Guide, Interactive PDF, Rev D Click to download [ Add to Cart (Free) ]
5405 Bio-Plex Multiplex System Brochure, Rev D Click to download [ Add to Cart (Free) ]
6054 Bio-Plex Pro TGF-beta Assays Product Information Sheet, Rev B Click to download [ Add to Cart (Free) ]
10024984 Instruction Manual, Bio-Plex Pro TGF-β Assays, Rev A Click to download
10024986 Instruction Manual, Bio-Plex Pro Assays Quick Guide 5, Rev A Click to download
6457 The Bio-Plex Success Guide for Multiplex Immunoassays, Rev B Click to download
6458 Bio-Plex Success Guide/Assay Sample Prep, Rev B Click to download