Bio-Plex® 200 Systems

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Overview

Bio-Plex Array Reader Design

APD: Avalanche photodiode detector

DD: Doublet discriminator channel, discriminates single beads from aggregated beads

CL1: Classify channel, allows multiplexing, detects dye inside beads

RP1: Reporter channel, quantitates assay in this channel


Bio-Plex assays are bead-based assays that can be performed in a mixed array (multiplexed). The reader combines two lasers, fluidics, and real-time digital signal processing to distinguish up to 100 different sets of color-coded polystyrene beads, each bearing a different assay. The Bio-Plex array reader is an essential tool that performs the key functions of this multiplex technology:

  • Fluidics — The Bio-Plex array reader detects individual beads by flow cytometry. The fluidics system of the reader aligns the beads into single file as they enter a stream of sheath fluid and then enter a flow cell. Once the beads are in single file within the flow cell, each bead is individually interrogated for bead color (analyte) and assay signal strength (PE fluorescence intensity)
  • Lasers — The Bio-Plex array reader is a dual laser system. The 532 nm Nd-Yag laser (green "reporter" laser) is used to excite the PE dye of the assay, for example, streptavidin-PE used for sandwich immunoassays. The 635 nm solid state laser (red "classify" laser) is used to excite the dyes inside the beads to determine their "color" or "region" and is also used for doublet discrimination by light scatter
  • Optics — The Bio-Plex array reader contains an optics bench with four detection channels. Details are shown below. The dual lasers are precisely focused to excite an individual bead within the quartz flow cell. Upon excitation, the fluorescent signal emitted from the bead travels though the optics paths to the individual detectors
  • Detectors — The Bio-Plex array reader has four detectors, one for each of the optical paths shown in the figure below. A high-sensitivity photomultiplier tube (PMT) detector is used for the reporter channel. Photodiodes are used for the stronger signals of the classify CL1 and CL2 and doublet discrimination channels

The Bio-Plex 200 system offers simplified needle height adjustment, improved array reader and microplate platform alignment, a robust, quiet air compressor, and other significant advantages, including:

  • Simplified startup, shutdown, control, and maintenance routines
  • Streamlined data analysis with no need to export or import files
  • Automated installation and operational qualification (IQ/OQ)
  • Integrated system validation and calibration logs for a record of system performance
  • Superior curve fitting and statistics reporting
  • Calibration capabilities for both broad range and low range standard dilutions

The Bio-Plex® 200 system is a suspension array system which offers protein and nucleic acid researchers a reliable multiplex assay solution that permits analysis of up to 100 biomolecules in a single sample.

Included System Components

  • Array reader — combines 2 lasers, high-throughput fluidics, and real-time digital signal processing to distinguish up to 100 different color-coded bead sets, each representing a different assay
  • Microplate platform — automates the reading of 96-well plates, yielding up to 9,600 data points in about 35 min
  • Calibration and validation kits — system tools for daily calibration of signal output and for IQ/OQ and monthly validation of the primary performance parameters of the reader
  • Bio-Plex MCV plate IV — maintenance, calibration, and validation (MCV) plate customized for hands-free startup, shutdown, and performance testing
  • PC and monitor — includes Pentium 4 processor, CD-RW, Windows XP operating system, Microsoft Office Professional and Adobe Acrobat Reader software, and a 17" flat-screen monitor
  • Bio-Plex Manager™ software — controls the instrument, data acquisition, and data analysis for superior curve fitting, statistics reporting, and charting features, without requiring the exporting or importing of files
  • Bio-Plex® SNP Manager™ data analysis utility — simple-to-use data analysis tool for nucleic acid applications
Environment conditions  
Operating temperature 15–30°C (59–86°F)
Operating humidity, noncondensing 20–80%
Operating altitude Designed to operate at up to 2,000 m (6,600') mean sea level
Compensatory range ±2°C
UL installation category UL Installation Category II, as defined in Annex J of UL 3101–1
Pollution degree UL Pollution Degree 2, as defined in Section 3.7, 3.2, of UL 3101–1
Bio-Plex Array Reader Specifications  
Input voltage range 100–240 V, 1.5 A, 47 – 63 Hz
Physical dimensions (W x D x H), cm 43 x 51 x 23
Weight, kg/lb 23/60
Lasers  
Reporter laser 532 nm, >10 mW, frequency-doubled diode; 30 x 60 µm elliptical beam
Classification laser 635 nm, 10 mW, diode; 30 x 60 µm elliptical beam
Fluidics  
Sheath flow rate, µl/sec 90
Cuvette 200 µm square flow channel
Sample injection rates, µl/sec 60
Electronics  
Reporter channel detection Photomultiplier tube, A/D resolution 14 bits
Classification and doublet discriminator channel detection Avalanche photodiodes with temperature compensation, A/D resolution 12 bits
Communication interface RS–232
Signal Processing  
Measurement resolution 15 bits effective
Processor modes Linear, with logarithmic or linear display option
Dynamic range, dB 70
Bio-Plex Microplate Platform Specifications  
Input voltage range 100–240 V, 1.8 A, 47–63 Hz
Physical dimensions (W x D x H), cm 44 x 61 x 8
Weight, kg/lb 14.4 (32)
Communications interface RS–232
Plate capacity One 96-well microtiter plate, ≤0.75
Bio-Plex® 200 System

171-000201
100–240 V, multiplex array system, includes reader, microplate platform, Bio-Plex Manager™ 6.0 software, PC, calibration kit, validation kit 4.0, MCV plate IV, reservoir, sheath fluid, 2 sample needles

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Bio-Plex 200 System With HTF #171-000205
Bio-Plex® 200 System With HTF

171-000205
100–240 V, multiplex array system (#171-000201) with high-throughput fluidics

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Number Description Options
6006 Bio-Plex MAGPIX, Bio-Plex 200, and Bio-Plex 3D Systems – Comparable Performance Across the Board, Product Information Sheet, Rev A Click to download
6457 The Bio-Plex Success Guide for Multiplex Immunoassays, Rev B Click to download
5405 Bio-Plex Multiplex System Brochure, Rev D Click to download [ Add to Cart (Free) ]
6156 Composite Profiling of Angiogenic Factors Using Bio-Plex Pro Human Cancer Biomarker Panel 1, Rev A Click to download [ Add to Cart (Free) ]
3076 Bio-Plex Care and Maintenance Quick Guide, Rev B Click to download
2993 Bio-Plex Glossary, Rev A Click to download
4100205 Bio-Plex Maintenance Log, Rev A Click to download [ Add to Cart (Free) ]
5336 Bio-Plex Suspension Array System Nucleic Acid Analysis Flier, Rev B Click to download
3000 Correlation of Bio-Plex Suspension Array Reader Validation With Multiplex Cytokine Assay Performance, Rev A Click to download
10005042 Instruction Manual, Bio-Plex 200 System Hardware Instruction, Rev F Click to download
4100180 Instruction Manual, Bio-Plex Protein Array System High Throughput Fluidics (HTF) System, Rev A Click to download [ Add to Cart (Free) ]
BR125FS Second Generation Multiplex Immunoassays Click to download
5924 Stem Cell Basics for Life Science Researchers Brochure, Rev A Click to download [ Add to Cart (Free) ]
3013 Versatility of the Bio-Plex System: Application to the Detection of Bacterial Protein Toxins, Rev A Click to download
5578 Xenograft Samples By Bio-Plex Tech note, Rev A Click to download
2879 Bio-Plex System Tools Brochure, Rev C Click to download
6458 Bio-Plex Success Guide/Assay Sample Prep, Rev B Click to download