Overview
This one-step reverse transcription digital PCR supermix delivers improved efficiency, specificity, and sensitivity for precise RNA target quantification with Droplet Digital PCR.
Key Benefits
- Absolute quantification by Droplet Digital PCR in a convenient single-reaction format
- Contains all components required for hydrolysis probe–based RT-ddPCR except primers, probe(s), and template
- Limits nonspecific PCR amplification
- Optimized enzyme blend enables partitioning of RNA samples into droplets while keeping the enzymes inactive until the reverse transcription reaction is performed at 50°C
- Contains RNase inhibitor to protect the RNA throughout the entire workflow
Applications and Uses
- Absolute quantification
- Gene expression analysis
- Viral load detection
- Pathogen detection
Packaging Options
Additional Reagents
Bio-Rad offers additional consumables for completing your digital PCR workflow:
- Digital PCR consumables
- PrimePCR ddPCR Assays
Additional Equipment
This digital PCR supermix requires the use of one or more of the following instruments and accessories:
- Automated Droplet Generator
- QX200 Droplet Generator
- QX200 Droplet Reader
- QX 600 Droplet Reader
- QX One Droplet Digital PCR System
- PX1 PCR Plate Sealer
- PTC Tempo Deepwell Thermal Cycler or C1000 Touch Thermal Cycler with 96–Deep Well Reaction Module
More Information
The ddPCR Supermix for Residual Quantification is optimized for use with the Droplet Generation Oil for Probes on the QX200 Droplet Digital PCR System, QX200 AutoDG Droplet Digital System, QX600 Droplet Digital PC System, QX600 AutoDG Droplet Digital System, and QX one Droplet Digital PCR System.
Specifications
Ordering
items
Use the filters below to refine results!
200 x 20 µl reaction kit, for absolute quantification of target RNA in the QX600/QX200 Droplet Digital™ PCR Systems
500 x 20 µl reaction kit, for absolute quantification of target RNA in the QX600/QX200 Droplet Digital™ PCR Systems
Consumables
70 ml (10 x 7 ml), oil for use with droplet generator in the QX600/QX200 Droplet Digital™ PCR Systems
2 L (2 x 1 L), oil for use with droplet reader in the QX600/QX200 Droplet Digital™ PCR Systems
Pkg of 24, cartridges for use with the QX200 Droplet Generator
Pkg of 24, gaskets for use with QX200 Droplet Generator
5 pkg of 24 DG8 Cartridges, 5 pkg of 24 DG8 Gaskets, for use with the QX200 Droplet Generator
Equipment
View AllQX600 AutoDG Droplet Digital PCR System enables and automates up to 6-color multiplexing for up to 96-samples per run providing clear discrimination of multiple targets. System includes Automated Droplet Generator, QX600 Droplet Reader, computer, and QX Manager Software, Standard Edition.
Droplet Digital PCR System, includes droplet generator, droplet reader, computer, software, associated component consumables, for EvaGreen or probe-based digital PCR applications
AutoDG Instrument for automated droplet generation of up to 96 samples, for use with the QX600/QX200 Droplet Digital PCR Systems
Droplet generator for EvaGreen or probe-based digital PCR applications, for use with QX200™ Droplet Digital™ PCR System
Droplet reader for EvaGreen or probe-based digital PCR applications, for use with the QX200 Droplet Digital™ System
Modular thermal cycler platform, includes C1000 Touch Thermal Cycler chassis, 96–deep well reaction module, USB flash drive