Criterion™ TGX™ Precast Gels

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lsr_criterion_tgx_precastgels.jpg

Overview

Criterion TGX precast gels are based on the long shelf life TGX formulation. The gels deliver superior resolution, fast run times, and greater transfer efficiencies.
Read full description Ordering Information

Description

Description

The Criterion™ TGX™ (Tris-Glycine eXtended) precast gels for PAGE are based on the long shelf life TGX formulation. The TGX gels retain Laemmli-like separation characteristics using the standard sample and Tris/Glycine running buffers. The proteins in the gel can be separated in as little as 20 min at 300 V while maintaining low temperatures.

Benefits Include:

  • Run time as short as 20 min
  • Protein transfer in as little as 15 min
  • Direct drop-in for Laemmli systems
  • Inexpensive Laemmli buffer system, low running costs
  • High-throughput system

TGX gels are manufactured without SDS. The gels can be used to run proteins under nondenaturing conditions for subsequent analysis of their native conformation and activity by omitting SDS from the running buffer. Native analysis does not provide accurate molecular weight information. SDS is added to the sample and running buffer to create standard SDS denaturing electrophoresis conditions.

Criterion gels are packaged and sold in individual units: 1–2 gels can be run in the Criterion cell and 1–12 gels can be run in the high-throughput Criterion™ Dodeca™ cell.

 

Specifications

Specifications

Gel dimensions (Width x Length x Thickness)
133 x 87 x 1 mm
Cassette dimensions (Width x Length x Thickness)
150 x 106 x 5.3 mm
Cassette material
Styrene copolymer
Comb material
Polycarbonate
Gel storage conditions
Store flat at 4°C; do not freeze
Shelf life at 2-8°C*
12 months
Recommended sample buffer (Laemmli, dilute 1:1 with sample)
62.5 mM Tris-HCl, pH 6.8, 2% SDS, 25% glycerol, 0.01% bromophenol blue
Recommended running buffer (Tris/glycine/SDS)
25 mM Tris, 192 mM glycine, 0.1% SDS, pH 8.3
Run times at constant voltage: 200 V
42–50 min
Run times at constant voltage: 300 V
20–26 min

Ordering

Ordering

items

Use the filters below to refine results!

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5671023
Pkg of 1, 7.5% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
567-1xxx_tn.jpg
5671024
Pkg of 1, 7.5% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
567-1xxx_tn.jpg
5671025
Pkg of 1, 7.5% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
567-1xxx_tn.jpg
5671033
Pkg of 1, 10% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
567-1xxx_tn.jpg
5671034
Pkg of 1, 10% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
567-1xxx_tn.jpg
5671035
Pkg of 1, 10% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
567-1xxx_tn.jpg
5671043
Pkg of 1, 12% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
567-1xxx_tn.jpg
5671044
Pkg of 1, 12% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
567-1xxx_tn.jpg
5671045
Pkg of 1, 12% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
567-1xxx_tn.jpg
5671071
Pkg of 1, 18% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
567-1xxx_tn.jpg
5671072
Discontinued item
Replaced with 10–20% Criterion™ TGX™ Precast Midi Protein Gel, Prep+2 well, 700 µl (5671112) or 18% Criterion™ TGX Stain-Free™ Protein Gel, Prep+2 well, 700 µl (5678072)
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5671073
Discontinued item
Replaced with 12% Criterion™ TGX™ Gel 12+2 well 45 µl (5671043) or 10–20% Criterion™ TGX™ Precast Midi Protein Gel, 12+2 well, 45 µl (5671113)
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5671074
Discontinued item
Replaced with 12% Criterion™ TGX™ Precast Midi Protein Gel, 18-well, 30 µl (5671044)
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5671075
Discontinued item
Replaced with 12% Criterion™ TGX™ Precast Midi Protein Gel, 26-well, 15 µl (5671045)
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5671081
Pkg of 1, 4–15% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
567-1xxx_tn.jpg
5671082
Pkg of 1, 4–15% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
567-1xxx_tn.jpg
5671083
Pkg of 1, 4–15% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
567-1xxx_tn.jpg
5671084
Pkg of 1, 4–15% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
567-1xxx_tn.jpg
5671085
Pkg of 1, 4–15% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
567-1xxx_tn.jpg
5671091
Pkg of 1, 4–20% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
567-1xxx_tn.jpg
5671092
Pkg of 1, 4–20% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
567-1xxx_tn.jpg
5671093
Pkg of 1, 4–20% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
567-1xxx_tn.jpg
5671094
Pkg of 1, 4–20% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
567-1xxx_tn.jpg
5671095
Pkg of 1, 4–20% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
567-1xxx_tn.jpg
5671101
Pkg of 1, 8–16% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
567-1xxx_tn.jpg
5671102
Pkg of 1, 8–16% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
567-1xxx_tn.jpg
5671103
Pkg of 1, 8–16% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
567-1xxx_tn.jpg
5671104
Pkg of 1, 8–16% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
567-1xxx_tn.jpg
5671105
Pkg of 1, 8–16% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
567-1xxx_tn.jpg
5671111
Pkg of 1, 10–20% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
567-1xxx_tn.jpg
5671112
Pkg of 1, 10–20% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
567-1xxx_tn.jpg
5671113
Pkg of 1, 10–20% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
567-1xxx_tn.jpg
5671114
Discontinued item
Replaced with Any kD™ Criterion™ TGX™ Precast Midi Protein Gel, 18-well, 30 µl (5671124)
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5671115
Discontinued item
Replaced with Any kD™ Criterion™ TGX™ Precast Midi Protein Gel, 26-well, 15 µl (5671125)
567-1xxx_tn.jpg
5671121
Pkg of 1, AnykD precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
567-1xxx_tn.jpg
5671122
Pkg of 1, AnykD precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
567-1xxx_tn.jpg
5671123
Pkg of 1, AnykD precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
567-1xxx_tn.jpg
5671124
Pkg of 1, AnykD precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
567-1xxx_tn.jpg
5671125
Pkg of 1, AnykD precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

Accessories

Accessories

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(Discontinued) Criterion™ Cell
1656001
Vertical midi-format electrophoresis cell, includes buffer tank, lid with power cables, 3 sample loading guides (12+2-well, 18-well, and 26-well)
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1654130
Vertical midi-format electrophoresis cell, includes buffer tank with built-in cooling coil, lid with power cables, 12 gel capacity
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4560000
Pkg of 1, 100% aluminum and recyclable opening lever for Mini-PROTEAN precast gel cassettes

Kits & Reagents

Kits & Reagents

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1610737
30 ml, premixed protein sample buffer for SDS-PAGE
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1610738
30 ml, premixed protein sample buffer for native PAGE, contains 62.5 mM Tris-HCl, pH 6.8, 40% glycerol, 0.01% bromophenol blue
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(Discontinued) 10x Tris/Glycine/SDS
1610732
Pkg of 1, 1 L, 10x premixed electrophoresis buffer, contains 25 mM Tris, 192 mM glycine, 0.1% SDS, pH 8.3 following dilution to 1x with water
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1610376
250 μl, pre-stained, Strep-tagged recombinant proteins (10–250 kD), including three pink reference bands (25, 50, 75 kD), 50 applications
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1610363
1 ml, unstained mixture of ten Strep-tagged, recombinant proteins (10–250 kD), including three reference bands (25, 50, 75 kD), 100 applications
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(Discontinued) 2-Mercaptoethanol
1610710
25 ml, ≥98% pure 2-mercaptoethanol (14.2 M)
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(Discontinued) Dithiothreitol (DTT)
1610610
1 g, dithiothreitol (DTT) reducing agent
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(Discontinued) Dithiothreitol (DTT)
1610611
5 g, dithiothreitol (DTT) reducing agent

Documents

Documents

Number Description Options
6507
TGX and TGX Stain-Free Gels: Publications List
6040
Electrophoresis Guide, Interactive PDF, Rev C
5981
Criterion Tris-HCl to Criterion TGX and TGX Stain-Free Precast Gels Catalog Number Conversion Chart
4110001
Instruction Manual, Criterion™ Gel Application Guide, Ver H
6493
Bio-Rad’s Host Cell Protein (HCP) Workflow Quick Guide
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