Criterion TGX™ Precast Gels

Criterion TGX precast gels are based on the long shelf life TGX formulation. The gels deliver superior resolution, fast run times, and greater transfer efficiencies.

Ordering Information

Overview

The Criterion TGX™ (Tris-Glycine eXtended) precast gels for PAGE are based on the long shelf life TGX formulation. The TGX gels retain Laemmli-like separation characteristics using the standard sample and Tris/Glycine running buffers. The proteins in the gel can be separated in as little as 20 min at 300 V while maintaining low temperatures.

Benefits Include:

Run time as short as 20 min
Protein transfer in as little as 15 min
Direct drop-in for Laemmli systems
Inexpensive Laemmli buffer system, low running costs
High-throughput system

TGX gels are manufactured without SDS. The gels can be used to run proteins under nondenaturing conditions for subsequent analysis of their native conformation and activity by omitting SDS from the running buffer. Native analysis does not provide accurate molecular weight information. SDS is added to the sample and running buffer to create standard SDS denaturing electrophoresis conditions.

Criterion gels are packaged and sold in individual units: 1–2 gels can be run in the Criterion cell and 1–12 gels can be run in the high-throughput Criterion™ Dodeca™ cell.

Related Technologies

Specifications

Gel dimensions (Width x Length x Thickness)

133 x 87 x 1 mm

Cassette dimensions (Width x Length x Thickness)

150 x 106 x 5.3 mm

Cassette material

Styrene copolymer

Comb material

Polycarbonate

Gel storage conditions

Store flat at 4°C; do not freeze

Shelf life at 2-8°C*

12 months

Recommended sample buffer (Laemmli, dilute 1:1 with sample)

62.5 mM Tris-HCl, pH 6.8, 2% SDS, 25% glycerol, 0.01% bromophenol blue

Recommended running buffer (Tris/glycine/SDS)

25 mM Tris, 192 mM glycine, 0.1% SDS, pH 8.3

Run times at constant voltage: 200 V

42–50 min

Run times at constant voltage: 300 V

20–26 min

Ordering

items

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(Discontinued) 7.5% Criterion™ TGX™ Precast Midi Precast Midi Protein Gel, 12+2 well, 45 µl

5671023

Pkg of 1, 7.5% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

(Discontinued) 7.5% Criterion™ TGX™ Precast Midi Protein Gel, 18 well, 30 µl

5671024

Pkg of 1, 7.5% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

(Discontinued) 7.5% Criterion™ TGX™ Precast Midi Protein Gel, 26 well, 15 µl

5671025

Pkg of 1, 7.5% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

(Discontinued) 10% Criterion™ TGX™ Precast Midi Protein Gel, 12+2 well, 45 µl

5671033

Pkg of 1, 10% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

(Discontinued) 10% Criterion™ TGX™ Precast Midi Protein Gel, 18 well, 30 µl

5671034

Pkg of 1, 10% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

(Discontinued) 10% Criterion™ TGX™ Precast Midi Protein Gel, 26 well, 15 µl

5671035

Pkg of 1, 10% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

(Discontinued) 12% Criterion™ TGX™ Precast Midi Protein Gel, 12+2 well, 45 µl

5671043

Pkg of 1, 12% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

(Discontinued) 12% Criterion™ TGX™ Precast Midi Protein Gel, 18 well, 30 µl

5671044

Pkg of 1, 12% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

(Discontinued) 12% Criterion™ TGX™ Precast Midi Protein Gel, 26 well, 15 µl

5671045

Pkg of 1, 12% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

(Discontinued) 18% Criterion™ TGX™ Precast Midi Protein Gel, 11 cm IPG/prep+1 well, 550 µl

5671071

Pkg of 1, 18% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

(Discontinued) 4–15% Criterion™ TGX™ Precast Midi Protein Gel, 11 cm IPG/prep+1 well, 550 µl

5671081