Criterion Precast Gels

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Overview

The precast gels available for the Criterion system are 13.3 x 8.7 cm polyacrylamide gels in single-use cassettes. This gel size provides reproducible, high-resolution results and fast setup.
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Description

Description

The precast gels available for the Criterion™ system are 13.3 x 8.7 cm polyacrylamide gels in single-use cassettes. This gel size provides reproducible, high-resolution results with fast setup, loading, and run times.

For 2-D applications, the Criterion IPG combs are designed to hold 11 cm ReadyStrip™ IPG strips, which provide 35% more focusing area in the first-dimension separation and 20% more gel area in the second dimension than mini systems using 7 cm strips. A sample well for protein standards is also included.

Criterion precast gels for 2-D applications are available in TGX™, TGX Stain-Free™, Bis-Tris, Tris-acetate, and Tris-HCl formulations.

The new Criterion™ TGX™ (Tris-Glycine eXtended) precast gels for PAGE are based on the long shelf life TGX formulation. The TGX gels retain Laemmli-like separation characteristics using the standard sample and Tris/glycine running buffers. The proteins in the gel can be separated in as little as 20 min at 300 V while maintaining low temperatures.

TGX and Tris-HCl gels are manufactured without SDS. These gels can be used to run proteins under nondenaturing conditions for subsequent analysis of their native conformation and activity by omitting SDS from the running buffer. SDS is added to the sample and running buffer to create standard SDS denaturing electrophoresis conditions.

Criterion gels are packaged and sold in individual units: 1–2 gels can be run in the Criterion cell and 1–12 gels can be run in the high-throughput Criterion™ Dodeca™ cell.

Specifications

Specifications

Gel dimensions (Width x Length x Thickness)
133 x 87 x 1 mm
Cassette dimensions (Width x Length x Thickness)
150 x 106 x 53 mm
Cassette material
Styrene copolymer
Comb material
Polycarbonate
Gel storage conditions
Store flat at 4°C; do not freeze
Shelf life at 2-8°C*
12 months
Recommended sample buffer (dilute 1:1 with sample)
Laemmli sample buffer: 62.5mM Tris-HCl, pH 6.8, 2% SDS, 25% Glycerol, 0.01% Bromophenol Blue
Recommended running buffer
Tris/Glycine/SDS running buffer: 25mM Tris, 192mM Glycine, 0.1% SDS, pH 8.3
Run times at constant voltage: 200 V
42-50 min
Run times at constant voltage: 300 V
20-26 min

Ordering

Ordering

items

Use the filters below to refine results!

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3450127
Pkg of 1, 4–12% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells
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3450115
Pkg of 1, 10% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells
345-0121_tn.jpg
3450121
Pkg of 1, 12% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells
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3450133
Pkg of 1, 3-8% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells
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3450101
Pkg of 1, 10% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells
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3450106
Discontinued item
Replaced with Any kD™ Criterion™ TGX™ Gel 11 cm IPG/prep+1 550 µl (5671121)
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3450107
Pkg of 1, 10–20% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells
345-0102_tn.jpg
3450102
Pkg of 1, 12.5% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells
345-0103_tn.jpg
3450103
Discontinued item
Replaced with 4–15% Criterion™ TGX™ Precast Midi Protein Gel, 11 cm IPG/prep+1 well, 550 µl (5671081)
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3450105
Discontinued item
Replaced with 8–16% Criterion™ TGX™ Precast Midi Protein Gel, 11 cm IPG/prep+1 well, 550 µl (5671101)
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3450104
Pkg of 1, 4–20% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells

Accessories

Accessories

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(Discontinued) Criterion™ Cell
1656001
Vertical midi-format electrophoresis cell, includes buffer tank, lid with power cables, 3 sample loading guides (12+2-well, 18-well, and 26-well)
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1654130
Vertical midi-format electrophoresis cell, includes buffer tank with built-in cooling coil, lid with power cables, 12 gel capacity

Kits & Reagents

Kits & Reagents

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1610737
30 ml, premixed protein sample buffer for SDS-PAGE
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1610738
30 ml, premixed protein sample buffer for native PAGE, contains 62.5 mM Tris-HCl, pH 6.8, 40% glycerol, 0.01% bromophenol blue
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(Discontinued) 10x Tris/Glycine/SDS
1610732
Pkg of 1, 1 L, 10x premixed electrophoresis buffer, contains 25 mM Tris, 192 mM glycine, 0.1% SDS, pH 8.3 following dilution to 1x with water
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1610376
250 μl, pre-stained, Strep-tagged recombinant proteins (10–250 kD), including three pink reference bands (25, 50, 75 kD), 50 applications
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1610363
1 ml, unstained mixture of ten Strep-tagged, recombinant proteins (10–250 kD), including three reference bands (25, 50, 75 kD), 100 applications

Documents

Documents

Number Description Options
2788
Criterion Blotting Tray Insert, Rev A
2710
Criterion™ Precast Gel System Brochure, Rev C
5981
Criterion Tris-HCl to Criterion TGX and TGX Stain-Free Precast Gels Catalog Number Conversion Chart
4110001
Instruction Manual, Criterion™ Gel Application Guide, Ver H