Unstained Protein Standards

Image
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Overview

Precision Plus Protein™ Standards for protein electrophoresis contain ten highly purified recombinant proteins with molecular masses from 10 to 250 kD.
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Description

Description

Free Protein Standards Selection Magnet

Free Protein Standards Selection Magnet

Place this handy selection guide magnet on your refrigerator or lab bench to eliminate the guesswork and make protein standard selection simple.

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Precision Plus Protein™ Standards for protein electrophoresis offer unsurpassed band sharpness, accurate MW determination, lot-to-lot consistency, and excellent blotting results. These standards contain ten highly purified recombinant proteins with molecular masses from 10 to 250 kD. These unstained protein standards include three high-intensity reference bands (25, 50, and 75 kD).

Precision Plus Protein Unstained Standards contain the unique Strep-tag affinity peptide, which allows detection and molecular weight determination on western blots. These standards offer absolute molecular weight accuracy confirmed by mass spectrometry, with a known amount of protein in each band to allow approximation of protein concentration in your sample. These protein standards are compatible with Laemmli and neutral pH buffer systems and can be used for accurate molecular weight determination on gels and blots.

Precision Plus Protein Unstained Standards are also an excellent choice for use with stain-free gels and the
Gel Doc™ EZ imaging system.

All Precision Plus Protein Standards generate a standard curve with r2 values >0.99, demonstrating a strong linear relationship between the proteins' log molecular weight and relative migration distance on a gel.

Specifications

Specifications

MW size range 
10–250 kD 
Stain type
Unstained
Volume
1 ml
Number of applications
100
Loading buffer
30% (w/v) glycerol, 2% SDS,
62.5 mM Tris, pH 6.8, 50 mM DTT,
5 mM EDTA, 0.02% NaN3,
0.01% bromophenol blue
Storage  
Store at –20ºC until expiration date (24 months from date of manufacture)

Ordering

Ordering

items

Use the filters below to refine results!

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1610363
1 ml, unstained mixture of ten Strep-tagged, recombinant proteins (10–250 kD), including three reference bands (25, 50, 75 kD), 100 applications
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1610396
5 ml (5 x 1 ml), unstained mixture of ten Strep-tagged, recombinant proteins (10–250 kD), including three reference bands (25, 50, 75 kD), 500 applications
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1610378
Pkg of 24, 1 mm thick agarose plugs containing ten Strep-tagged, recombinant proteins (10–250 kD), including three reference bands
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1610380
300 μl, StrepTactin and horseradish peroxidase (HRP) conjugate used for detection of Precision Plus Protein unstained standards, 150 applications
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1610381
125 μl, StrepTactin and horseradish peroxidase (HRP) conjugate used for detection of Precision Plus Protein unstained standards, 50 applications
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1610382
300 μl, StrepTactin and alkaline phosphatase (AP) conjugate used for detection of Precision Plus Protein unstained standards, 150 applications

Documents

Documents

Number Description Options
6533
Introducing Bio-Rad’s TGX and TGX Stain-Free FastCast Acrylamide Kits, Rev A
2895
Protein Blotting Guide, Ver C
6040
Electrophoresis Guide, Interactive PDF, Rev C
2032
Western Blotting Detection Reagents Brochure, Rev F
3036
Precision Plus Protein Plugs Promotional Card, Rev C
3117
Blotting Starter Kits, Rev B
2414
The Little Book of Standards, Rev D
3118
Protein Standards for Electrophoresis and Blotting Brochure, Rev D
3133
Molecular Weight Determination by SDS-PAGE, Rev B
2998
Protein Standards Application Guide, Rev G
[ Add to Cart (Free) ]
3093
Precision Plus Protein Standards Competitive Comparison Card, Rev B
3144
Using Precision Plus Protein Standards to Determine Molecular Weight, Rev B
5561
Precision Plus Protein™ WesternC™ Standards, Rev E
6148
Transfer of High Molecular Weight Proteins to Membranes: A Comparison of Transfer Efficiency Between Blotting Systems, Rev B
4110000
Instruction Manual, Precision StrepTactin HRP-Conjugate and AP-Conjugate, Rev D
4117684
Instruction Manual, Precision Plus Protein™ Standard Plugs, Unstained, Rev C
4110023
Instruction Manual, Unstained Precision Plus Protein™ Standards, Rev B