Description
Description
Choose midi Criterion XT Bis-Tris Protein Gels for versatile SDS PAGE. This gel can be run with two different denaturing running buffers, XT MES and XT MOPS, providing different separation profiles.
- Use of different running buffers to expand the utility of this gel
- Neutral pH (pH 6.4) delays acrylamide hydrolysis as compared to traditional Laemmli systems
- 12-month shelf life
- Run 2-D electrophoresis in less than a day
- One well for markers or other reference
The two running buffers and three different percentage XT Bis-Tris gels combine to provide six different migration patterns.
Running Buffer | Optimum Separation Ranges | ||
10% | 12% | 4–12% | |
XT MES | 2.5–200 kD | 1–66 kD | 2.5–225 kD |
XT MOPS | 14–220 kD | 6–100 kD | 10–300 kD |
Product Options
Criterion XT Bis-Tris Midi Protein Gels are available with a selection of different well configurations.
Well Configuration, Volume | Catalog Number | ||
10% | 12% | 4–12% | |
12+2 well, 45 µl | 3450111 | 3450117 | 3450123 |
18 well, 30 µl | 3450112 | 3450118 | 3450124 |
26 well, 15 µl | 3450113 | 3450119 | 3450125 |
Prep+2 well, 700 µl | – | 3450120 | 3450126 |
11 cm IPG/prep+1 well, 550 µl |
3450115 | 3450121 | 3450127 |
Related Products
- XT MOPS Running Buffer, 20x (1610788)
- XT MES Running Buffer, 20x (1610789)
- XT Sample Buffer, 4x (1610791)
- XT Reducing Agent, 20x (1610792)
- XT MOPS Buffer Kit (1610793) XT MOPS running buffer, XT sample buffer, and XT reducing agent
- XT MES Buffer Kit (1610796) XT MES running buffer, XT sample buffer, and XT reducing agent
Related Categories
- ReadyStrip™ IPG Strips
- PROTEAN® i12™ IEF System
- Protein Ladders and Standards (Markers) including unstained, prestained, and western standards
- Criterion Midi-Format Vertical Electrophoresis Cells, for 1 to 4 gels
- Criterion Dodeca Cell, for up to 12 gels
- ChemiDoc™ and GelDoc™ Imaging Systems
Supporting Documents
- Criterion XT Precast Gel Instruction Manual
- Criterion Gel Application Guide
- Criterion XT Precast Gels Product Information Sheet
- Criterion Precast Gel System Brochure
- Electrophoresis Guide, Interactive PDF
- PROTEAN i12 IEF System Brochure
- ReadyStrip IPG Strip Product Information Sheet