CHEF-DR II System

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CHEF-DR II System

Overview

Introduction to Pulsed Field Gel Electrophoresis
PFGE Standards Selection Guide
5 kb Ladder 8–48 kb Ladder Lambda Ladder S. cerevisiae
Chromosomal DNA
H. wingei
Chromosomal DNA
S. pombe
Chromosomal DNA
Comparison of Pulsed Field Standards
 
  Range Contents Amount Number of Applications
 
5 kb ladder 4.9–120 kb Concatemers of pBR328 20 µg in 200 µl 20–25
8–48 kb ladder 8.3–48.5 kb Mixed digest of phage λ 25 µg in 125 µl 125
Lambda (λ) ladder 0.05–1 Mb Concatemers of phage λcl857Sam7 5 agarose blocks 25–40
S. cerevisiae 0.225–2.2 Mb Saccharomyces cerevisiae chromosomal DNA 5 agarose blocks 25–40
H. wingei 1–3.1 Mb Hansenula wingei chromosomal DNA 5 agarose blocks 25–40
S. pombe 3.5–5.7 Mb Schizosaccharomyces pombe chromosomal DNA 5 agarose blocks 25–40
 

 

Certified Agarose Selection Guide
 
Packaging
25 g 1613100 1613103 1613106  1613108 1613111 1613113 1620137
100 g             1620138
125 g 1613101 1613107  1613109 1613112 1613114  
500 g 1613102 1613105    1613110   1613115  
Analytical Separation
≥1,000 bp          
≤1,000 bp          
10–800 bp            
1 kb–2 Mb          
1 kb–5 Mb          
Preparative Separation
Quantum Prep Methods    
Low-Melt Methods          
Agarose Methods          
In-Gel Applications          
Other Applications
Postpreparative Enzymatic Treatments    
Tissue/Cell Culture          
Pulsed Field Sample Preparation          
Blotting      

The CHEF-DR II system has been streamlined by condensing the functions of Pulsewave 760 technology and the power supply into the CHEF-DR II control module.
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Ordering Information


  • Description
  • Specifications
  • Ordering
  • Accessories
  • Kits & Reagents
  • Combs
  • Standards
  • Documents

Description

CHEF technology yields straight lanes over a wide range of DNA molecule sizes. The CHEF-DR® II system has been streamlined by condensing the functions of Pulsewave 760 technology and the power supply into the CHEF-DR II control module.

The CHEF-DR II system effectively resolves DNA fragments in the range of 5 kb to 6 Mb. This system lets you enhance resolution by entering two blocks of running conditions to be executed successively. The CHEF-DR II system exclusively employs the most common angle of electrophoresis for PFGE, 120°. This system is simple to program, and has proven to be a solid performer. Each CHEF-DR II system is supplied with an electrophoresis cell, drive module, control module, variable-speed pump, and accessory kit. The CHEF-DR II chiller systems also come with a cooling module.

Automation
The CHEF-DR II system, the most cost-effective PFGE instrument, requires direct user input of optimal run time, voltage gradient, and switch intervals for each run.

Customization
Users can program run conditions into the CHEF-DR II system. The product manual features extensive examples of run conditions that can be input based on a variety of size separation ranges.

Application Versatility
The CHEF-DR II system is optimized for the most popular range of separations with a fixed angle of 120°. This unit can be used for separations of fragments up to 6 Mb by adjusting the run conditions for low voltage and extended run times.


Specifications

Drive Module  
Dimensions (W x D x H) 43 x 26 x 14 cm
Construction Aluminum chassis
Weight 7.8 kg (17.2 lb)
Maximum voltage 250 V DC
Maximum current 500 mA
Electrode potentials Dynamically regulated (feedback adjustment) +/- 0.5%
Input voltage range 90–132 VAC/47–63 Hz/0.7 A (maximum)
198–264 VAC/47–63 Hz/0.35 A (maximum)
Fuses 0.5 A Fast Blow for high voltage output (front panel)
1.0 A Slow-Blow (100/120 V) or
0.5 A Slow-Blow (220/240 V)
Environmental  
Operating temperature 50°F (10°C) to 90°F (32°C); 30–80% humidity
Storage temperature 32°F (0°C) to 140°F (60°C); 10–90% humidity
Control Module  
Dimensions (W x D x H) 31 x 13.5 x 17 cm
Maximum current 500 mA
Allowable voltage gradients 0.6–6 V/cm, in 0.1 V/cm increments
Data entry Keyboard
Power supply 250 V maximum, to allow maximum gradient of 6 V/cm, continuously adjustable
Functional  
Switching range 0.1–65,000 sec (all electronic switching)
Maximum program blocks 2, with automatic execution
Maximum run time 999 hr per block
Input voltage range 90–132 VAC/47–63 Hz/1.7 A (maximum)
198–264 VAC/47–63 Hz/0.85 A (maximum)
Fuses   2.0 A Slow-Blow (100/120 V) or
1.0 A Slow-Blow (220/240 V)   
Operating   50°F (10°C) to 90°F (32°C); 30–80% humidity  
Storage   32°F (0°C) to 140°F (60°C); 10–90% humidity  
Electrophoresis Cell  
Dimensions (W x D x H) 44 x 43 x 11 cm, horizontal format
Construction Acrylic
Lid Safety interlocked
Weight 8.5 kg (18.7 lb)
Electrodes 24, platinum (0.01" diameter)
Temperature monitoring Via precision temperature probe mounted through lid (optional)
Accessories Included  
Variable speed pump 120 V, ground isolated; flow rate 0.75 liter/min, typical
Casting stand 14 x 13 cm
Comb 10 well comb and comb holder
Tygon tubing 365 cm
Sample plug mold 10 slot
Yeast DNA Standard S. cerevisiae YNN295, 2 plugs
Chromosomal Grade Agarose 5 g
Pulsed Field Certified Agarose 5 g
Leveling bubble 1
Fuses 0.5 A Fast Blow, 2
Manual 1
Cooling Module (optional)  
Chassis Aluminum
Dimensions (W x L x H) 23 x 42 x 24 cm
Weight 14 kg (30.1 lb)
Compressor 1/12 HP
Refrigerant CFC 12
Heat exchanger Stainless steel
Operating range  5°C–25°C 
Cooling rate (pre-cool)   0.75°C/min from ambient to 14°C 
Cooling capacity (input power)  75 W at 14°C 
Input voltage range  90–132 VAC/47–63 Hz/4 A
198–264 VAC/47–63 Hz/2 A 
Fuses  6.3 A Slow-Blow (100/120 V) or
3.0 A Slow-Blow (220/240 V) 

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(Discontinued) CHEF-DR II Chiller System

1703725
120 V, complete PFGE system with cooling module, pump, gel casting accessories, 2 plugs S. cerevisiae DNA size standard, 5 g pulsed field Certified agarose, 5 g pulsed field Certified megabase agarose

This product is no longer available. Please contact technical support for more information on replacement product.

The following replacement is recommended:

  • CHEF Mapper XA Chiller System, 120 V (1703670)

Accessories

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CHEF Electrophoresis Cell

1703649
Horizontal electrophoresis cell for use with with CHEF Mapper XA, CHEF DR-II, and CHEF DR-III Variable Angle systems

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$8,605.00 
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CHEF Cooling Module, 120 V

1703654
pulsed field gel electrophoresis cooling system, for use with all 120 V CHEF chiller systems

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$5,244.00 
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CHEF Cooling Module, 100 V

1703688
pulsed field gel electrophoresis cooling system, for use with all 100 V CHEF chiller systems

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$5,145.00 
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CHEF Cooling Module, 220/240 V

1703655
pulsed field gel electrophoresis cooling system, for use with all 220/240 V CHEF chiller systems

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$5,145.00 
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CHEF Variable-Speed Pump

1703644
120 V, pulsed field gel electrophoresis pump, for use with any CHEF system

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$1,206.00 
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CHEF Electrodes

1703648
Pkg of 6, thick gauge (0.02"), pulsed field gel electrophoresis electrodes, for use with any CHEF system

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$445.00 
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CHEF Screened Caps

1703711
Pkg of 5, pulsed field gel electrophoresis sample preparation accessory for use with any CHEF system

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$70.00 
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CHEF Disposable Plug Molds, 50-Well

1703713
Pkg of 5x50 pulsed field gel electrophoresis disposable plug molds, for use with any CHEF system

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$71.00 
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CHEF Reusable Plug Mold

1703622
Pkg of 1, 10 plug mold for PFGE, for use with any CHEF system

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$220.00 
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CHEF Standard Casting Stand

1703689
Pkg of 1, pulsed field gel electrophoresis casting stand, includes 14 x 13 cm frame and platform, for use with any CHEF system

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$346.00 
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CHEF Wide/Long Combination Casting Stand

1703704
Pkg of 1, pulsed field gel electrophoresis casting stand, includes 21 x 14 cm frame and platform, for use with any CHEF system

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$555.00 
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CHEF Leveling Table

1704046
Pkg of 1, gel-casting level surface, 20 x 30 cm, for use with the CHEF Mapper XA chiller system

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$462.00 
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Gel Scoop

1703643
Pkg of 1, pulsed field gel electrophoresis gel scoop, for use with any CHEF system

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$97.00 
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Kits & Reagents

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Certified Megabase Agarose, 25 g

1613108
25 g, agarose for use with CHEF and FIGE applications, certified free of inhibitors, DNases, RNases

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$105.00 
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Certified Megabase Agarose, 125 g

1613109
125 g, agarose for use with CHEF and FIGE applications, certified free of inhibitors, DNases, RNases

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$349.00 
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Certified Megabase Agarose, 500 g

1613110
500 g, agarose for use with CHEF and FIGE applications, certified free of inhibitors, DNases, RNases

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$1,082.00 
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Certified Molecular Biology Agarose, 25 g

1613100
25 g, general purpose agarose for nucleic acid electrophoresis, high gel strength, certified free of inhibitors, DNases, RNases

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$70.00 
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Certified Molecular Biology Agarose, 125 g

1613101
125 g, general purpose agarose for nucleic acid electrophoresis, high gel strength, certified free of inhibitors, DNases, RNases

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$213.00 
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Certified Molecular Biology Agarose, 500g

1613102
500 g, general purpose agarose for nucleic acid electrophoresis, high gel strength, certified free of inhibitors, DNases, RNases

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$689.00 
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Pulsed Field Certified Agarose, 100 g

1620137
100 g, agarose for separation of large DNA in pulsed field gel applications, certified free of inhibitors, DNases, RNases

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$281.00 
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Pulsed Field Certified Agarose, 500 g

1620138
500 g, agarose for separation of large DNA in pulsed field gel applications, certified free of inhibitors, DNases, RNases

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$1,238.00 
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10x TBE (Tris/Boric Acid/EDTA) Buffer, 1 L

1610733
1 L, TBE nucleic acid electrophoresis buffer solution, pH 8.3

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$43.00 
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10x TBE (Tris/Boric Acid/EDTA) Buffer, 5 L

1610770
5 L cube, TBE nucleic acid electrophoresis buffer solution, pH 8.3

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$132.00 
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50x TAE (Tris/Acetic Acid/EDTA) Buffer, 1 L

1610743
1 L, TAE nucleic acid electrophoresis buffer solution, pH 8.0

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$100.00 
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50x TAE (Tris/Acetic Acid/EDTA) Buffer, 5 L

1610773
5 L cube, TAE nucleic acid electrophoresis buffer solution, pH 8.0

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$294.00 
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Ethidium Bromide Solution, 10 mg/mL

1610433
10 mL ethidium bromide solution for fluorescent staining of nucleic acids, 10 mg/mL

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$46.00 
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Hazardous  
EDTA

1610729
500 g, ≥99% pure EDTA (disodium ethylenediaminetetraacetate dihydrate) powder

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$82.00 
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Tris

1610716
500 g, ≥99.8% pure Tris base (Tris[hydroxymethyl]aminomethane or Trimethamine)

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$37.00 
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Tris

1610719
1 kg, ≥99.8% pure Tris base (Tris[hydroxymethyl]aminomethane or Trimethamine)

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Combs

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10-Well Comb, 14 cm x 1.5 mm

1704326
Adjustable-height comb, for use with Sub-Cell and Wide Mini-Sub Cell GT Systems (requires comb holder #1704320) or CHEF-DR and CHEF Mapper Systems (requires comb holder #1703699)

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$70.00 
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10-Well Comb, 14 cm x 0.75 mm

1704325
Adjustable-height comb, for use with Sub-Cell and Wide Mini-Sub Cell GT Systems (requires comb holder #1704320) or CHEF-DR and CHEF Mapper Systems (requires comb holder #1703699)

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$70.00 
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15-Well Comb , 14 cm x 1.5 mm

1704324
Adjustable-height comb, for use with Sub-Cell and Wide Mini-Sub Cell GT Systems (requires comb holder #1704320) or CHEF-DR and CHEF Mapper Systems (requires comb holder #1703699)

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$70.00 
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15-Well Comb, 14 cm x 0.75 mm

1704323
Adjustable-height comb, for use with Sub-Cell and Wide Mini-Sub Cell GT Systems (requires comb holder #1704320) or CHEF-DR and CHEF Mapper Systems (requires comb holder #1703699)

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$66.00 
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20-Well Comb, 14 cm x 1.5 mm

1704322
Adjustable-height comb, for use with Sub-Cell and Wide Mini-Sub Cell GT Systems (requires comb holder #1704320) or CHEF-DR and CHEF Mapper Systems (requires comb holder #1703699)

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$70.00 
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30-Well Comb, 14 cm x 1.5 mm

1704344
Adjustable-height comb, for use with Sub-Cell and Wide Mini-Sub Cell GT Systems (requires comb holder #1704320) or CHEF-DR and CHEF Mapper Systems (requires comb holder #1703699)

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$71.00 
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15-Well Comb, 21 cm x 1.5 mm

1703627
Pkg of 1, 15-well pulsed field gel electrophoresis comb, for use with CHEF-DR® and CHEF Mapper® Systems (requires comb holder #1703699) or any CHEF system

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$145.00 
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30-Well Comb, 21 cm x 1.5 mm

1703628
Pkg of 1, 30-well pulsed field gel electrophoresis comb, for use with CHEF-DR® and CHEF Mapper® Systems (requires comb holder #1703699) or any CHEF system

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$151.00 
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45-Well Comb, 21 cm x 1.5 mm

1703645
Pkg of 1, 45-well pulsed field gel electrophoresis comb, for use with CHEF-DR® and CHEF Mapper® Systems (requires comb holder #1703699) or any CHEF system

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$136.00 
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(Discontinued) Preparative Comb, 14 cm x 1.5 mm

1703623
Pkg of 1, preparative comb with 2 outer wells for size standards, for use with CHEF-DR® and CHEF Mapper® Systems (requires comb holder #1703699) or any CHEF system

This product is no longer available. Please contact technical support for more information on replacement product.
Combination Comb Holder

1703699
Pkg of 1, pulsed field gel electrophoresis comb holder, for use with any CHEF system

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$213.00 
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Standards

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CHEF DNA Size Standard, 4.9–98 kb Ladder

1703624
200 μl, 0.1 μg/μl, PFGE standard, 5 kb ladder, 4.9–120 kb, concatemers of pBR328, 20–25 applications

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$256.00 
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CHEF DNA Size Standard, 8.3–48.5 kb, Lambda Ladder

1703707
125 μl, 0.2 μg/μl, PFGE standard, 8.3–48.5 kb, mixed digests of phage lambda (λ), 125 applications

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$106.00 
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CHEF DNA Size Standard, 48.5–1,000 kb, Lambda Ladder

1703635
5 agarose blocks, PFGE standard, lambda (λ) ladder, 48.5-1000 kb in 48.5 kb increments, concatemers of λcl857Sam7 lambda DNA per block, for 25–40 plugs

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$514.00 
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CHEF DNA Size Marker, 0.2–2.2 Mb, S. cerevisiae Ladder

1703605
5 agarose blocks, PFGE marker, 0.225–2.2 Mb S. cerevisiae chromosomal DNA, for 25–40 plugs

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$185.00 
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CHEF DNA Size Marker, 1–3.1 Mb, H. wingei Ladder

1703667
5 agarose blocks, PFGE marker, 1–3.1 Mb, H. wingei chromosomal DNA, for 25–40 plugs

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$197.00 
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(Discontinued) CHEF DNA Size Marker

1703633
5 agarose blocks, PFGE marker, 3.5–5.7 Mb, S. pombe chromosomal DNA, for 25–40 plugs

This product is no longer available. Please contact technical support for more information on replacement product.

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Documents

Number Description Options
M1703729 Instruction Manual, CHEF-DR II Pulsed Field Electrophoresis Systems, Rev B Click to download
5901 Pulsed Field Gel Electrophoresis Brochure, Rev B Click to download
4000066 Instruction Manual, Cooling Module Click to download
1736 PFGE Analysis of Chromosomal DNA From a Single Hair Root Click to download
1647 Electrophoretic Karyotypes of Wine Strains of Saccharomyces cerevisiae Click to download
1648 Application of Two-Dimensional Pulsed Field Electrophoresis for Determining Molecular Karyotypes Bulletin, Rev A Click to download
1769 Resolution of Linear DNA Fragments From 23 Kilobases to 6 Megabases Using Biphasic Linear Switch-Time Ramping Click to download
1589 Preparation and Separation of Mammalian DNA by Pulsed Field Gel Electrophoresis Bulletin, Rev A Click to download
9204213 Instruction Manual, CHEF Variable Speed Pump, Rev B Click to download
LIT526 Instruction Manual, Standard Casting Stand Combination Comb Holder Wide/Long Combination Casting Stand Click to download
1624 Model 1000 Mini Chiller Product Information Sheet Click to download
6228 Using Pulsed Field Gel Electrophoresis Standards, Rev A Click to download

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