CHEF-DR II System

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Overview

The CHEF-DR II system has been streamlined by condensing the functions of Pulsewave 760 technology and the power supply into the CHEF-DR II control module.
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Description

Description

CHEF technology yields straight lanes over a wide range of DNA molecule sizes. The CHEF-DR® II system has been streamlined by condensing the functions of Pulsewave 760 technology and the power supply into the CHEF-DR II control module.

The CHEF-DR II system effectively resolves DNA fragments in the range of 5 kb to 6 Mb. This system lets you enhance resolution by entering two blocks of running conditions to be executed successively. The CHEF-DR II system exclusively employs the most common angle of electrophoresis for PFGE, 120°. This system is simple to program, and has proven to be a solid performer. Each CHEF-DR II system is supplied with an electrophoresis cell, drive module, control module, variable-speed pump, and accessory kit. The CHEF-DR II chiller systems also come with a cooling module.

Automation
The CHEF-DR II system, the most cost-effective PFGE instrument, requires direct user input of optimal run time, voltage gradient, and switch intervals for each run.

Customization
Users can program run conditions into the CHEF-DR II system. The product manual features extensive examples of run conditions that can be input based on a variety of size separation ranges.

Application Versatility
The CHEF-DR II system is optimized for the most popular range of separations with a fixed angle of 120°. This unit can be used for separations of fragments up to 6 Mb by adjusting the run conditions for low voltage and extended run times.

Specifications

Specifications

Drive Module
 
Dimensions (W x D x H)
43 x 26 x 14 cm
Construction
Aluminum chassis
Weight
7.8 kg (17.2 lb)
Maximum voltage
250 V DC
Maximum current
500 mA
Electrode potentials
Dynamically regulated (feedback adjustment) +/- 0.5%
Input voltage range
90–132 VAC/47–63 Hz/0.7 A (maximum)
198–264 VAC/47–63 Hz/0.35 A (maximum)
Fuses
0.5 A Fast Blow for high voltage output (front panel)
1.0 A Slow-Blow (100/120 V) or
0.5 A Slow-Blow (220/240 V)
Environmental
 
Operating temperature
50°F (10°C) to 90°F (32°C); 30–80% humidity
Storage temperature
32°F (0°C) to 140°F (60°C); 10–90% humidity
Control Module
 
Dimensions (W x D x H)
31 x 13.5 x 17 cm
Maximum current
500 mA
Allowable voltage gradients
0.6–6 V/cm, in 0.1 V/cm increments
Data entry
Keyboard
Power supply
250 V maximum, to allow maximum gradient of 6 V/cm, continuously adjustable
Functional
 
Switching range
0.1–65,000 sec (all electronic switching)
Maximum program blocks
2, with automatic execution
Maximum run time
999 hr per block
Input voltage range
90–132 VAC/47–63 Hz/1.7 A (maximum)
198–264 VAC/47–63 Hz/0.85 A (maximum)
Fuses  
2.0 A Slow-Blow (100/120 V) or
1.0 A Slow-Blow (220/240 V)   
Operating  
50°F (10°C) to 90°F (32°C); 30–80% humidity  
Storage  
32°F (0°C) to 140°F (60°C); 10–90% humidity  
Electrophoresis Cell
 
Dimensions (W x D x H)
44 x 43 x 11 cm, horizontal format
Construction
Acrylic
Lid
Safety interlocked
Weight
8.5 kg (18.7 lb)
Electrodes
24, platinum (0.01" diameter)
Temperature monitoring
Via precision temperature probe mounted through lid (optional)
Accessories Included
 
Variable speed pump
120 V, ground isolated; flow rate 0.75 liter/min, typical
Casting stand
14 x 13 cm
Comb
10 well comb and comb holder
Tygon tubing
365 cm
Sample plug mold
10 slot
Yeast DNA standard
S. cerevisiae YNN295, 2 plugs
Chromosomal grade agarose
5 g
Pulsed field Certified agarose
5 g
Leveling bubble
1
Fuses
0.5 A Fast Blow, 2
Manual
1
Cooling Module (optional)
 
Chassis
Aluminum
Dimensions (W x L x H)
23 x 42 x 24 cm
Weight
14 kg (30.1 lb)
Compressor
1/12 HP
Refrigerant
CFC 12
Heat exchanger
Stainless steel
Operating range 
5°C–25°C 
Cooling rate (pre-cool)  
0.75°C/min from ambient to 14°C 
Cooling capacity (input power) 
75 W at 14°C 
Input voltage range 
90–132 VAC/47–63 Hz/4 A
198–264 VAC/47–63 Hz/2 A 
Fuses 
6.3 A Slow-Blow (100/120 V) or
3.0 A Slow-Blow (220/240 V) 

Ordering

Ordering

items

Use the filters below to refine results!

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1703725
120 V, complete PFGE system with cooling module, pump, gel casting accessories, 2 plugs S. cerevisiae DNA size standard, 5 g pulsed field Certified agarose, 5 g pulsed field Certified megabase agarose
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1703727
220/240 V, complete PFGE system with cooling module, pump, gel casting accessories, 2 plugs S. cerevisiae DNA size standard, 5 g pulsed field Certified agarose, 5 g pulsed field Certified megabase agarose

Accessories

Accessories

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1703689
Pkg of 1, pulsed field gel electrophoresis casting stand, includes 14 x 13 cm frame and platform, for use with any CHEF system
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1703704
Pkg of 1, pulsed field gel electrophoresis casting stand, includes 21 x 14 cm frame and platform, for use with any CHEF system

Kits & Reagents

Kits & Reagents

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1613100
25 g, general purpose agarose for nucleic acid electrophoresis, high gel strength, certified free of inhibitors, DNases, RNases
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1613101
125 g, general purpose agarose for nucleic acid electrophoresis, high gel strength, certified free of inhibitors, DNases, RNases
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1613102
500 g, general purpose agarose for nucleic acid electrophoresis, high gel strength, certified free of inhibitors, DNases, RNases
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1620137
100 g, agarose for separation of large DNA in pulsed field gel applications, certified free of inhibitors, DNases, RNases
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1620138
500 g, agarose for separation of large DNA in pulsed field gel applications, certified free of inhibitors, DNases, RNases

Combs

Combs

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1704326

For use with Sub-Cell and Wide Mini-Sub Cell GT Systems (requires comb holder #1704320) or CHEF-DR and CHEF Mapper Systems (requires comb holder #1703699)

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1704325

For use with Sub-Cell and Wide Mini-Sub Cell GT Systems (requires comb holder #1704320) or CHEF-DR and CHEF Mapper Systems (requires comb holder #1703699)

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1704324

For use with Sub-Cell and Wide Mini-Sub Cell GT Systems (requires comb holder #1704320) or CHEF-DR and CHEF Mapper Systems (requires comb holder #1703699)

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1704323

For use with Sub-Cell and Wide Mini-Sub Cell GT Systems (requires comb holder #1704320) or CHEF-DR and CHEF Mapper Systems (requires comb holder #1703699)

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1704322

For use with Sub-Cell and Wide Mini-Sub Cell GT Systems (requires comb holder #1704320) or CHEF-DR and CHEF Mapper Systems (requires comb holder #1703699)

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1704344

For use with Sub-Cell and Wide Mini-Sub Cell GT Systems (requires comb holder #1704320) or CHEF-DR and CHEF Mapper Systems (requires comb holder #1703699)

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1703627
Pkg of 1, 15-well pulsed field gel electrophoresis comb, for use with CHEF-DR® and CHEF Mapper® Systems (requires comb holder #1703699) or any CHEF system
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1703628
Pkg of 1, 30-well pulsed field gel electrophoresis comb, for use with CHEF-DR® and CHEF Mapper® Systems (requires comb holder #1703699) or any CHEF system
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1703645
Pkg of 1, 45-well pulsed field gel electrophoresis comb, for use with CHEF-DR® and CHEF Mapper® Systems (requires comb holder #1703699) or any CHEF system
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1703623
Pkg of 1, preparative comb with 2 outer wells for size standards, for use with CHEF-DR® and CHEF Mapper® Systems (requires comb holder #1703699) or any CHEF system

Standards

Standards

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1703624
200 μl, 0.1 μg/μl, PFGE standard, 5 kb ladder, 4.9–120 kb, concatemers of pBR328, 20–25 applications
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1703707
125 μl, 0.2 μg/μl, PFGE standard, 8.3–48.5 kb, mixed digests of phage lambda (λ), 125 applications
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1703635
5 agarose blocks, PFGE standard, lambda (λ) ladder, 48.5-1000 kb in 48.5 kb increments, concatemers of λcl857Sam7 lambda DNA per block, for 25–40 plugs
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1703605
5 agarose blocks, PFGE marker, 0.225–2.2 Mb S. cerevisiae chromosomal DNA, for 25–40 plugs
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(Discontinued) CHEF DNA Size Marker
1703633
5 agarose blocks, PFGE marker, 3.5–5.7 Mb, S. pombe chromosomal DNA, for 25–40 plugs

Documents

Documents

Number Description Options
M1703729
Instruction Manual, CHEF-DR II Pulsed Field Electrophoresis Systems, Rev B
5901
Pulsed Field Gel Electrophoresis Brochure, Rev B
4000066
Instruction Manual, Cooling Module
1736
PFGE Analysis of Chromosomal DNA From a Single Hair Root
1647
Electrophoretic Karyotypes of Wine Strains of Saccharomyces cerevisiae
1648
Application of Two-Dimensional Pulsed Field Electrophoresis for Determining Molecular Karyotypes Bulletin, Rev A
1769
Resolution of Linear DNA Fragments From 23 Kilobases to 6 Megabases Using Biphasic Linear Switch-Time Ramping
1589
Preparation and Separation of Mammalian DNA by Pulsed Field Gel Electrophoresis Bulletin, Rev A
9204213
Instruction Manual, CHEF Variable Speed Pump, Rev B
LIT526
Instruction Manual, Standard Casting Stand Combination Comb Holder Wide/Long Combination Casting Stand
1624
Model 1000 Mini Chiller Product Information Sheet
6228
Using Pulsed Field Gel Electrophoresis Standards, Rev A