CHEF Genomic DNA Plug Kits

Overview

Separation of E. coli Chromosomal Restriction Fragments

Separation of E. coli chromosomal restriction fragments. Lanes 1 and 14, S. cerevisiae chromosomes; lanes 2 and 13, lambda ladder; lanes 3–6, NotI restriction digests of chromosomal DNA from DH5αF' (lane 3), HB101 (lane 4), MC1061 (lane 5), MV1190 (lane 6); lanes 8–11, SfiI restriction digests of chromosomal DNA from DH5αF' (lane 8), HB101 (lane 9), MC1061 (lane 10), MV1190 (lane 11). DNA fragments were separated on a 1.0% pulsed field certified agarose gel in 0.5x TBE at 14°C for 26.4 hr at 6 V/cm (200 V) using a 120° included angle with a 2.77–26.32 sec linear switch-time ramp.

PFGE Standards Selection Guide


5 kb Ladder	8–48 kb Ladder	Lambda Ladder	S. cerevisiae Chromosomal DNA	H. wingei Chromosomal DNA	S. pombe Chromosomal DNA

Comparison of Pulsed Field Standards

	Range	Contents	Amount	Number of Applications

5 kb ladder	4.9–120 kb	Concatemers of pBR328	20 µg in 200 µl	20–25
8–48 kb ladder	8.3–48.5 kb	Mixed digest of phage λ	25 µg in 125 µl	125
Lambda (λ) ladder	0.05–1 Mb	Concatemers of phage λcl857Sam7	5 agarose blocks	25–40
S. cerevisiae	0.225–2.2 Mb	Saccharomyces cerevisiae chromosomal DNA	5 agarose blocks	25–40
H. wingei	1–3.1 Mb	Hansenula wingei chromosomal DNA	5 agarose blocks	25–40
S. pombe	3.5–5.7 Mb	Schizosaccharomyces pombe chromosomal DNA	5 agarose blocks	25–40

Certified Agarose Selection Guide

	Molecular Biology Agarose	PCR Agarose	Low Range Ultra Agarose	Megabase Agarose	Low-Melt Agarose	PCR Low-Melt Agarose	Pulse Field Agarose
Packaging
25 g	1613100	1613103	1613106	1613108	1613111	1613113	1620137
100 g							1620138
125 g	1613101		1613107	1613109	1613112	1613114
500 g	1613102	1613105		1613110		1613115
Analytical Separation
≥1,000 bp	•				•
≤1,000 bp		•				•
10–800 bp			•
1 kb–2 Mb				•			•
1 kb–5 Mb				•			•
Preparative Separation
Quantum Prep Methods	•	•	•	•			•
Low-Melt Methods					•	•
Agarose Methods					•	•
In-Gel Applications					•	•
Other Applications
Postpreparative Enzymatic Treatments	•	•	•		•	•
Tissue/Cell Culture				•			•
Pulsed Field Sample Preparation				•			•
Blotting	•	•		•			•

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CHEF genomic DNA plug kits provide a convenient means for preparing intact, chromosome-sized DNA for PFGE. With such large molecules, pipetting mechanically shears the DNA, resulting in unacceptable quality for PFGE separations. This problem has required procedures for lysis and deproteinization of whole cells embedded in agarose to yield intact purified DNA. Three kits are available for the preparation of bacterial (lysozyme-sensitive) or mammalian genomic DNA and yeast chromosomes (YACs). Each kit contains all the enzymes, reaction buffers, and restriction digest-qualified CleanCut agarose necessary to prepare 100 plugs, as well as disposable plug molds and screened caps for simplified plug processing. Each kit is thoroughly tested to ensure that prepared genomic DNA can be restriction digested and separated on a CHEF electrophoresis system.

CHEF Mammalian Genomic DNA Plug Kit
Cell suspension buffer	12 ml
>600 U/ml proteinase K	1.3 ml
Proteinase K reaction buffer	30 ml
2% CleanCut™ agarose	12 ml
10x wash buffer	60 ml
50-well disposable plug mold	2
Screened cap	1
CHEF Bacterial Genomic DNA Plug Kit
Cell suspension buffer	12 ml
>600 U/ml proteinase K	1.3 ml
Proteinase K reaction buffer	30 ml
2% CleanCut agarose	12 ml
10x wash buffer	60 ml
25 mg/ml lysozyme	1.6 ml
Lysozyme buffer	30 ml
50-well disposable plug mold	2
Screened cap	1
CHEF Yeast Genomic DNA Plug Kit
Cell suspension buffer	12 ml
>600 U/ml proteinase K	1.3 ml
Proteinase K reaction buffer	30 ml
2% CleanCut agarose	12 ml
10x wash buffer	60 ml
5,000 U/ml lyticase	1.6 ml
Lyticase buffer	30 ml
50-well disposable plug mold	2
Screened cap	1

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CHEF Mammalian Genomic DNA Plug Kit

1703591
Mammalian preparation kit for PFGE, includes proteinase K, CleanCut Agarose, all required buffers, screened cap, 2 plug molds, for 100 plugs

0 1703591 /templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Nucleic-Acid-Electrophoresis--/170-3591_0921120435513.xml 1703591 /default/main/bio-rad/WORKAREA/default 09/21/09 12:04 PM en_US jthvle4t 2017-11-14 07:08:14 true true internet/product_SKU jthvle4t templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Nucleic-Acid-Electrophoresis--/170-3591_0921120435513.xml LSR 2019-05-04 20:19:59 /default/main/bio-rad/STAGING N /default/main/bio-rad en 12/31/37 11:59 PM 1556003273455 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN 2019-05-04 20:19:59 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Nucleic-Acid-Electrophoresis--/170-3591_0921120435513.xml templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Nucleic-Acid-Electrophoresis--/170-3591_0921120435513.xml 1703591-chef-mammalian-genomic-dna-plug-kit 1703591 CHEF Mammalian Genomic DNA Plug Kit CHEF Mammalian Genomic DNA Plug Kit Mammalian preparation kit for PFGE, includes proteinase K, CleanCut Agarose, all required buffers, screened cap, 2 plug molds, for 100 plugs Mammalian preparation kit for PFGE, includes proteinase K, CleanCut Agarose, lyticase, all required buffers, screened cap, 2 plug molds, for 100 plugs Mammalian preparation kit for PFGE, includes proteinase K, CleanCut Agarose, all required buffers, screened cap, 2 plug molds, for 100 plugs /webroot/web/images/lsr/products/electrophoresis/sku_thumb/global/170-3591_tn.jpg /webroot/web/images/lsr/products/electrophoresis/sku_view/global/170-3591_view.jpg /webroot/web/images/lsr/products/electrophoresis/sku_feature/global/170-3591_feature.jpg preview ,VN RepEx 170-3591 Mammalian preparation kit for PFGE, includes proteinase K, CleanCut Agarose, lyticase, all required buffers, screened cap, 2 plug molds, for 100 plugs 1703591, chef, mammalian, genomic, dna, plug, sample, preparation, pfge, pulsed, field, gel, electrophoresis 09/21/09 12:04 PM 12/31/37 11:59 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSR /LSR/Electrophoresis-and-Blotting/Nucleic-Acid-Electrophoresis-- N 0 1703594 /templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Nucleic-Acid-Electrophoresis--/170-3594_0921120435495.xml 1703605 /templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Nucleic-Acid-Electrophoresis--/170-3605_0921120431250.xml false $392.00 false $ null true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1703591&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1703591&CartQuote=QT&quantity= 1290 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1703591&AddToDefaultWishList=true&quantity= 41105300 392.00 1703591

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CHEF Bacterial Genomic DNA Plug Kit

1703592
Bacteria preparation kit for PFGE, includes proteinase K, CleanCut Agarose, lysozyme, required buffers, screened cap, 2 plug molds, for 100 plugs

1 1703592 /templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Nucleic-Acid-Electrophoresis--/170-3592_0921120435403.xml 1703592 /default/main/bio-rad/WORKAREA/default 09/21/09 12:04 PM en_US jthvle1d 2017-11-14 07:08:14 true true internet/product_SKU jthvle1d templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Nucleic-Acid-Electrophoresis--/170-3592_0921120435403.xml LSR 2019-05-04 20:19:48 /default/main/bio-rad/STAGING N /default/main/bio-rad en 12/31/37 11:59 PM 1556003273499 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN 2019-05-04 20:19:48 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Nucleic-Acid-Electrophoresis--/170-3592_0921120435403.xml templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Nucleic-Acid-Electrophoresis--/170-3592_0921120435403.xml 1703592-chef-bacterial-genomic-dna-plug-kit 1703592 CHEF Bacterial Genomic DNA Plug Kit CHEF Bacterial Genomic DNA Plug Kit Bacteria preparation kit for PFGE, includes proteinase K, CleanCut Agarose, lysozyme, required buffers, screened cap, 2 plug molds, for 100 plugs Bacteria preparation kit for PFGE, includes proteinase K, CleanCut Agarose, lysozyme, required buffers, screened cap, 2 plug molds, for 100 plugs Bacteria preparation kit for PFGE, includes proteinase K, CleanCut Agarose, lysozyme, required buffers, screened cap, 2 plug molds, for 100 plugs /webroot/web/images/lsr/products/electrophoresis/sku_thumb/global/170-3592_tn.jpg /webroot/web/images/lsr/products/electrophoresis/sku_view/global/170-3592_view.jpg /webroot/web/images/lsr/products/electrophoresis/sku_feature/global/170-3592_feature.jpg RepEx 170-3592 Bacteria preparation kit for PFGE, includes proteinase K, CleanCut Agarose, lysozyme, required buffers, screened cap, 2 plug molds, for 100 plugs 09/21/09 12:04 PM 12/31/37 11:59 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSR /LSR/Electrophoresis-and-Blotting/Nucleic-Acid-Electrophoresis-- N 0 1703592, chef, bacterial, genomic, dna, plug, sample, preparation, pfge, pulsed, field, gel, electrophoresis 1703605 /templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Nucleic-Acid-Electrophoresis--/170-3605_0921120431250.xml 1703594 /templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Nucleic-Acid-Electrophoresis--/170-3594_0921120435495.xml false $519.00 false $ null true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1703592&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1703592&CartQuote=QT&quantity= 1290 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1703592&AddToDefaultWishList=true&quantity= 41105300 519.00 1703592

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CHEF Yeast Genomic DNA Plug Kit

1703593
Yeast preparation kit for PFGE, includes proteinase K, CleanCut Agarose, lyticase, all required buffers, screened cap, and 2 plug molds, for 100 plugs

2 1703593 /templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Nucleic-Acid-Electrophoresis--/170-3593_0921120435590.xml 1703593 /default/main/bio-rad/WORKAREA/default 09/21/09 12:04 PM en_US jthvle9p 2017-11-14 07:08:14 true true internet/product_SKU jthvle9p templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Nucleic-Acid-Electrophoresis--/170-3593_0921120435590.xml LSR 2019-05-04 20:20:12 /default/main/bio-rad/STAGING N /default/main/bio-rad en 12/31/37 11:59 PM 1556003273541 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN 2019-05-04 20:20:12 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Nucleic-Acid-Electrophoresis--/170-3593_0921120435590.xml templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Nucleic-Acid-Electrophoresis--/170-3593_0921120435590.xml 1703593-chef-yeast-genomic-dna-plug-kit 1703593 CHEF Yeast Genomic DNA Plug Kit CHEF Yeast Genomic DNA Plug Kit Yeast preparation kit for PFGE, includes proteinase K, CleanCut Agarose, lyticase, all required buffers, screened cap, and 2 plug molds, for 100 plugs Yeast preparation kit for PFGE, includes proteinase K, CleanCut Agarose, lyticase, all required buffers, screened cap, and 2 plug molds, for 100 plugs Yeast preparation kit for PFGE, includes proteinase K, CleanCut Agarose, lyticase, all required buffers, screened cap, and 2 plug molds, for 100 plugs /webroot/web/images/lsr/products/electrophoresis/sku_thumb/global/170-3593_tn.jpg /webroot/web/images/lsr/products/electrophoresis/sku_view/global/170-3593_view.jpg /webroot/web/images/lsr/products/electrophoresis/sku_feature/global/170-3593_feature.jpg RepEx 170-3593 Yeast preparation kit for PFGE, includes proteinase K, CleanCut Agarose, lyticase, all required buffers, screened cap, and 2 plug molds, for 100 plugs 09/21/09 12:04 PM 12/31/37 11:59 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSR /LSR/Electrophoresis-and-Blotting/Nucleic-Acid-Electrophoresis-- N 0 1703593, chef, yeast, genomic, dna, plug, sample, preparation, pfge, pulsed, field, gel, electrophoresis 1703605 /templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Nucleic-Acid-Electrophoresis--/170-3605_0921120431250.xml 1703594 /templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Nucleic-Acid-Electrophoresis--/170-3594_0921120435495.xml false $595.00 false $ null true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1703593&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1703593&CartQuote=QT&quantity= 1290 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1703593&AddToDefaultWishList=true&quantity= 41105300 595.00 1703593

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CleanCut Agarose

1703594
12 ml, 2% agarose for pulsed field gel electrophoresis (PFGE), restriction digest-qualified, for 100 plugs

3 1703594 /templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Nucleic-Acid-Electrophoresis--/170-3594_0921120435495.xml 1703594 /default/main/bio-rad/WORKAREA/default 09/21/09 12:04 PM en_US jthvle6o 2017-11-14 07:08:14 true true internet/product_SKU jthvle6o templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Nucleic-Acid-Electrophoresis--/170-3594_0921120435495.xml LSR 2019-05-04 20:20:04 /default/main/bio-rad/STAGING N /default/main/bio-rad en 12/31/37 11:59 PM 1556003273583 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN 2019-05-04 20:20:04 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Nucleic-Acid-Electrophoresis--/170-3594_0921120435495.xml templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Nucleic-Acid-Electrophoresis--/170-3594_0921120435495.xml 1703594-cleancut-agarose 1703594 CleanCut Agarose CleanCut Agarose 12 ml, 2% agarose for pulsed field gel electrophoresis (PFGE), restriction digest-qualified, for 100 plugs 12 ml, 2% agarose for pulsed field gel electrophoresis (PFGE), restriction digest-qualified, for 100 plugs 12 ml, 2% agarose for pulsed field gel electrophoresis (PFGE), restriction digest-qualified, for 100 plugs /webroot/web/images/lsr/products/electrophoresis/sku_thumb/global/170-3594_tn.jpg /webroot/web/images/lsr/products/electrophoresis/sku_view/global/170-3594_view.jpg /webroot/web/images/lsr/products/electrophoresis/sku_feature/global/170-3594_feature.jpg RepEx 170-3594 12 ml, 2% agarose for pulsed field gel electrophoresis (PFGE), restriction digest-qualified, for 100 plugs 09/21/09 12:04 PM 12/31/37 11:59 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSR /LSR/Electrophoresis-and-Blotting/Nucleic-Acid-Electrophoresis-- N 0 1703594, cleancut, agarose, 2%, pulsed, field, gel, electrophoresis, pfge, restriction, digest-qualified 1703605 /templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Nucleic-Acid-Electrophoresis--/170-3605_0921120431250.xml false $34.00 false $ null true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1703594&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1703594&CartQuote=QT&quantity= 1290 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1703594&AddToDefaultWishList=true&quantity= 41105300 34.00 1703594

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CHEF Mapper XA Chiller System, 120 V

1703670
120 V, complete PFGE system, includes cooling module, pump, tubing, S. cerevisiae DNA size standard, 5 g pulsed field Certified agarose, 5 g pulsed field Certified megabase agarose

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CHEF-DR III Chiller System

1703700
120 V, complete variable angle PFGE system with cooling module, includes all gel casting accessories, pump, tubing, DNA standards, 2 plugs of S. cerevisiae DNA size standard, PFGE grade agarose

1703700 /templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Nucleic-Acid-Electrophoresis--/170-3700_0921120431209.xml 10 1703700 /default/main/bio-rad/WORKAREA/default 09/21/09 12:04 PM en_US jcc1tovd 2017-11-14 07:08:14 true true internet/product_SKU jcc1tovd templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Nucleic-Acid-Electrophoresis--/170-3700_0921120431209.xml LSR 2019-05-04 20:20:18 /default/main/bio-rad/STAGING N /default/main/bio-rad en 12/31/37 11:59 PM 1556003274423 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN 2019-05-04 20:20:18 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Nucleic-Acid-Electrophoresis--/170-3700_0921120431209.xml templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Nucleic-Acid-Electrophoresis--/170-3700_0921120431209.xml 1703700-chef-dr-iii-chiller-system 1703700 CHEF-DR III Chiller System CHEF-DR III Chiller System 120 V, complete variable angle PFGE system with cooling module, includes all gel casting accessories, pump, tubing, DNA standards, 2 plugs of <em>S. cerevisiae</em> DNA size standard, PFGE grade agarose 120 V, complete variable angle PFGE system with cooling module, includes all gel casting accessories, pump, tubing, DNA standards, 2 plugs of <em>S. cerevisiae</em> DNA size standard, PFGE grade agarose 120 V, complete variable angle PFGE system with cooling module, includes all gel casting accessories, pump, tubing, DNA standards, 2 plugs of <em>S. cerevisiae</em> DNA size standard, PFGE grade agarose /webroot/web/images/lsr/products/electrophoresis/sku_thumb/global/170-3700_tn.jpg /webroot/web/images/lsr/products/electrophoresis/sku_view/global/170-3700_view.jpg /webroot/web/images/lsr/products/electrophoresis/sku_feature/global/170-3700_feature.jpg RepEx 170-3700 120 V, complete variable angle PFGE system with cooling module, includes all gel casting accessories, pump, tubing, DNA standards, 2 plugs of <em>S. cerevisiae</em> DNA size standard, PFGE grade agarose 09/21/09 12:04 PM 12/31/37 11:59 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSR /LSR/Electrophoresis-and-Blotting/Nucleic-Acid-Electrophoresis-- N 0 1703700, chef-dr, iii, chiller, complete, variable, angle, pfge, pulsed, field, gel, electrophoresis, casting, accessories, reagents, instrument, 120, 120V, volts 1703591 /templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Nucleic-Acid-Electrophoresis--/170-3591_0921120435513.xml 1703592 /templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Nucleic-Acid-Electrophoresis--/170-3592_0921120435403.xml false null false $ null true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1703700&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1703700&CartQuote=QT&quantity= 1402 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1703700&AddToDefaultWishList=true&quantity= 41105300 null 1703700

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CHEF-DR II Chiller System

1703725
120 V, complete PFGE system with cooling module, pump, gel casting accessories, 2 plugs S. cerevisiae DNA size standard, 5 g pulsed field Certified agarose, 5 g pulsed field Certified megabase agarose

1703725 /templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Nucleic-Acid-Electrophoresis--/170-3725_0921120430970.xml 15 1703725 /default/main/bio-rad/WORKAREA/default 09/21/09 12:04 PM en_US jcc1towa 2017-11-14 07:08:14 true true internet/product_SKU jcc1towa templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Nucleic-Acid-Electrophoresis--/170-3725_0921120430970.xml LSR 2019-05-04 20:20:05 /default/main/bio-rad/STAGING N /default/main/bio-rad en 12/31/37 11:59 PM 1556003275505 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN 2019-05-04 20:20:05 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Nucleic-Acid-Electrophoresis--/170-3725_0921120430970.xml templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Nucleic-Acid-Electrophoresis--/170-3725_0921120430970.xml 1703725-chef-dr-ii-chiller-system 1703725 CHEF-DR II Chiller System CHEF-DR II Chiller System 120 V, complete PFGE system with cooling module, pump, gel casting accessories, 2 plugs <em>S. cerevisiae</em> DNA size standard, 5 g pulsed field Certified agarose, 5 g pulsed field Certified megabase agarose 120 V, complete PFGE system with cooling module, pump, gel casting accessories, 2 plugs <em>S. cerevisiae</em> DNA size standard, 5 g pulsed field Certified agarose, 5 g pulsed field Certified megabase agarose 120 V, complete PFGE system with cooling module, pump, gel casting accessories, 2 plugs <em>S. cerevisiae</em> DNA size standard, 5 g pulsed field Certified agarose, 5 g pulsed field Certified megabase agarose /webroot/web/images/lsr/products/electrophoresis/sku_thumb/global/170-3725_tn.jpg /webroot/web/images/lsr/products/electrophoresis/sku_view/global/170-3725_view.jpg /webroot/web/images/lsr/products/electrophoresis/sku_feature/global/170-3725_feature.jpg RepEx 170-3725 120 V, complete PFGE system with cooling module, pump, gel casting accessories, 2 plugs <em>S. cerevisiae</em> DNA size standard, 5 g pulsed field Certified agarose, 5 g pulsed field Certified megabase agarose 09/21/09 12:04 PM 12/31/37 11:59 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSR /LSR/Electrophoresis-and-Blotting/Nucleic-Acid-Electrophoresis-- N 0 1703725, chef-dr, ii, chiller, complete, pfge, pulsed, field, gel, electrophoresis, casting, accessories, reagents, instrument, 120, 120V, volts false null false $ null true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1703725&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1703725&CartQuote=QT&quantity= 1402 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1703725&AddToDefaultWishList=true&quantity= 41105300 null 1703725

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Number	Description	Options
1753	Preparation and Restriction Digestion of Escherichia coli Chromosomal DNA in Agarose Plugs for Use in PFGE Bulletin, Rev A	Click to download
LIT510	Instruction Manual, CHEF Genomic DNA Plug Kit, Rev E	Click to download
5901	Pulsed Field Gel Electrophoresis Brochure, Rev B	Click to download
6228	Using Pulsed Field Gel Electrophoresis Standards, Rev A	Click to download

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CHEF Genomic DNA Plug Kits

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CHEF Genomic DNA Plug Kits

Overview

CHEF Mammalian Genomic DNA Plug Kit

CHEF Bacterial Genomic DNA Plug Kit

CHEF Yeast Genomic DNA Plug Kit

CleanCut Agarose

CHEF Mapper XA Chiller System, 120 V

CHEF-DR III Chiller System

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I cannot find the Certificate of Analysis I am looking for. Help!

Where can I find the catalog number, SKU number, or product number?

Where can I find the lot or control number?

Why are Certificates of Analysis not on the Documents tab?

I have a batch number, not a lot/control number. How do I find the Certificate of Analysis for my item?

Can I get a Certificate of Analysis even if my item is expired?

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