CHEF Genomic DNA Plug Kits

CHEF Genomic DNA Plug Kits

Overview

CHEF genomic DNA plug kits provide a convenient means for preparing intact, chromosome-sized DNA for PFGE.
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Description

Description

CHEF genomic DNA plug kits provide a convenient means for preparing intact, chromosome-sized DNA for PFGE. With such large molecules, pipetting mechanically shears the DNA, resulting in unacceptable quality for PFGE separations. This problem has required procedures for lysis and deproteinization of whole cells embedded in agarose to yield intact purified DNA. Three kits are available for the preparation of bacterial (lysozyme-sensitive) or mammalian genomic DNA and yeast chromosomes (YACs). Each kit contains all the enzymes, reaction buffers, and restriction digest-qualified CleanCut agarose necessary to prepare 100 plugs, as well as disposable plug molds and screened caps for simplified plug processing. Each kit is thoroughly tested to ensure that prepared genomic DNA can be restriction digested and separated on a CHEF electrophoresis system.

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Specifications

Specifications

CHEF Mammalian Genomic DNA Plug Kit
 
Cell suspension buffer
12 ml     
>600 U/ml proteinase K
1.3 ml
Proteinase K reaction buffer
30 ml
2% CleanCut™ agarose
12 ml
10x wash buffer
60 ml
50-well disposable plug mold
2
Screened cap
1
CHEF Bacterial Genomic DNA Plug Kit
 
Cell suspension buffer 
12 ml 
>600 U/ml proteinase K 
1.3 ml  
Proteinase K reaction buffer 
30 ml 
2% CleanCut agarose 
12 ml    
10x wash buffer 
60 ml  
25 mg/ml lysozyme
1.6 ml     
Lysozyme buffer
30 ml  
50-well disposable plug mold 
2 
Screened cap 
1 
CHEF Yeast Genomic DNA Plug Kit
 
Cell suspension buffer  
12 ml  
>600 U/ml proteinase K     
1.3 ml  
Proteinase K reaction buffer  
30 ml  
2% CleanCut agarose  
12 ml    
10x wash buffer  
60 ml    
5,000 U/ml lyticase
1.6 ml   
Lyticase buffer
30 ml    
50-well disposable plug mold    
2    
Screened cap
1    

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170-3591_tn.jpg
(Discontinued) CHEF Mammalian Genomic DNA Plug Kit
1703591
Mammalian preparation kit for PFGE, includes proteinase K, CleanCut Agarose, all required buffers, screened cap, 2 plug molds, for 100 plugs
170-3592_tn.jpg
(Discontinued) CHEF Bacterial Genomic DNA Plug Kit
1703592
Bacteria preparation kit for PFGE, includes proteinase K, CleanCut Agarose, lysozyme, required buffers, screened cap, 2 plug molds, for 100 plugs
170-3593_tn.jpg
(Discontinued) CHEF Yeast Genomic DNA Plug Kit
1703593
Yeast preparation kit for PFGE, includes proteinase K, CleanCut Agarose, lyticase, all required buffers, screened cap, and 2 plug molds, for 100 plugs

Systems

Systems

170-3670_tn.jpg
(Discontinued) CHEF Mapper XA Chiller System, 120 V
1703670
120 V, complete PFGE system, includes cooling module, pump, tubing, S. cerevisiae DNA size standard, 5 g pulsed field Certified agarose, 5 g pulsed field Certified megabase agarose

Documents

Documents

Number Description Options
1753
Preparation and Restriction Digestion of Escherichia coli Chromosomal DNA in Agarose Plugs for Use in PFGE Bulletin, Rev A
LIT510
Instruction Manual, CHEF Genomic DNA Plug Kit, Rev E
5901
Pulsed Field Gel Electrophoresis Brochure, Rev B
6228
Using Pulsed Field Gel Electrophoresis Standards, Rev A