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      CHEF Genomic DNA Plug Kits

      lsr_chef_genomic_dna_plug_kits.jpg

      Overview

      CHEF genomic DNA plug kits provide a convenient means for preparing intact, chromosome-sized DNA for PFGE.
      Read full description Ordering Information

      Description

      Description

      CHEF genomic DNA plug kits provide a convenient means for preparing intact, chromosome-sized DNA for PFGE. With such large molecules, pipetting mechanically shears the DNA, resulting in unacceptable quality for PFGE separations. This problem has required procedures for lysis and deproteinization of whole cells embedded in agarose to yield intact purified DNA. Three kits are available for the preparation of bacterial (lysozyme-sensitive) or mammalian genomic DNA and yeast chromosomes (YACs). Each kit contains all the enzymes, reaction buffers, and restriction digest-qualified CleanCut agarose necessary to prepare 100 plugs, as well as disposable plug molds and screened caps for simplified plug processing. Each kit is thoroughly tested to ensure that prepared genomic DNA can be restriction digested and separated on a CHEF electrophoresis system.

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      Specifications

      Specifications

      CHEF Mammalian Genomic DNA Plug Kit
       
      Cell suspension buffer
      12 ml     
      >600 U/ml proteinase K
      1.3 ml
      Proteinase K reaction buffer
      30 ml
      2% CleanCut™ agarose
      12 ml
      10x wash buffer
      60 ml
      50-well disposable plug mold
      2
      Screened cap
      1
      CHEF Bacterial Genomic DNA Plug Kit
       
      Cell suspension buffer 
      12 ml 
      >600 U/ml proteinase K 
      1.3 ml  
      Proteinase K reaction buffer 
      30 ml 
      2% CleanCut agarose 
      12 ml    
      10x wash buffer 
      60 ml  
      25 mg/ml lysozyme
      1.6 ml     
      Lysozyme buffer
      30 ml  
      50-well disposable plug mold 
      2 
      Screened cap 
      1 
      CHEF Yeast Genomic DNA Plug Kit
       
      Cell suspension buffer  
      12 ml  
      >600 U/ml proteinase K     
      1.3 ml  
      Proteinase K reaction buffer  
      30 ml  
      2% CleanCut agarose  
      12 ml    
      10x wash buffer  
      60 ml    
      5,000 U/ml lyticase
      1.6 ml   
      Lyticase buffer
      30 ml    
      50-well disposable plug mold    
      2    
      Screened cap
      1    

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      170-3591_tn.jpg
      (Discontinued) CHEF Mammalian Genomic DNA Plug Kit
      1703591
      Mammalian preparation kit for PFGE, includes proteinase K, CleanCut Agarose, all required buffers, screened cap, 2 plug molds, for 100 plugs
      170-3592_tn.jpg
      (Discontinued) CHEF Bacterial Genomic DNA Plug Kit
      1703592
      Bacteria preparation kit for PFGE, includes proteinase K, CleanCut Agarose, lysozyme, required buffers, screened cap, 2 plug molds, for 100 plugs
      170-3593_tn.jpg
      (Discontinued) CHEF Yeast Genomic DNA Plug Kit
      1703593
      Yeast preparation kit for PFGE, includes proteinase K, CleanCut Agarose, lyticase, all required buffers, screened cap, and 2 plug molds, for 100 plugs

      Systems

      Systems

      170-3670_tn.jpg
      (Discontinued) CHEF Mapper XA Chiller System, 120 V
      1703670
      120 V, complete PFGE system, includes cooling module, pump, tubing, S. cerevisiae DNA size standard, 5 g pulsed field Certified agarose, 5 g pulsed field Certified megabase agarose
      170-3700_tn.jpg
      (Discontinued) CHEF-DR III Chiller System
      1703700
      120 V, complete variable angle PFGE system with cooling module, includes all gel casting accessories, pump, tubing, DNA standards, 2 plugs of S. cerevisiae DNA size standard, PFGE grade agarose
      The following replacement is recommended:
      • CHEF Mapper XA Chiller System, 120 V ( 1703670 )
      170-3725_tn.jpg
      (Discontinued) CHEF-DR II Chiller System
      1703725
      120 V, complete PFGE system with cooling module, pump, gel casting accessories, 2 plugs S. cerevisiae DNA size standard, 5 g pulsed field Certified agarose, 5 g pulsed field Certified megabase agarose
      The following replacement is recommended:
      • CHEF Mapper XA Chiller System, 120 V ( 1703670 )

      Documents

      Documents

      Number Description Options
      1753
      Preparation and Restriction Digestion of Escherichia coli Chromosomal DNA in Agarose Plugs for Use in PFGE Bulletin, Rev A
      LIT510
      Instruction Manual, CHEF Genomic DNA Plug Kit, Rev E
      5901
      Pulsed Field Gel Electrophoresis Brochure, Rev B
      6228
      Using Pulsed Field Gel Electrophoresis Standards, Rev A