Pulsed Field Standards and Agaroses

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Pulsed Field Standards and Agaroses

Overview

Introduction to Pulsed Field Gel Electrophoresis

This is from Product Overlay
PFGE Standards Selection Guide

Comparison of Pulsed Field Standards

  Range Contents Amount Number of Applications
5 kb ladder 4.9–120 kb Concatemers of pBR328 20 µg in 200 µl 20–25
8–48 kb ladder 8.3–48.5 kb Mixed digest of phage λ 25 µg in 125 µl 125
Lambda(λ) ladder 0.05–1 Mb Concatemers of phage λcl857Sam7 5 agarose blocks 25–40
S. cerevisiae 0.225–2.2 Mb Saccharomyces cerevisiae chromosomal DNA 5 agarose blocks 25–40
H. wingei 1–3.1 Mb Hansenula wingei chromosomal DNA 5 agarose blocks 25–40
S. pombe 3.5–5.7 Mb Schizosaccharomyces pombe chromosomal DNA 5 agarose blocks 25–40
Certified Agarose Selection Guide
 
Packaging
25 g 1613100 1613103 1613106  1613108 1613111 1613113 1620137
100 g             1620138
125 g 1613101 1613107  1613109 1613112 1613114  
500 g 1613102 1613105    1613110   1613115  
Analytical Separation
≥1,000 bp          
≤1,000 bp          
10–800 bp            
1 kb–2 Mb          
1 kb–5 Mb          
Preparative Separation
Quantum Prep Methods    
Low-Melt Methods          
Agarose Methods          
In-Gel Applications          
Other Applications
Postpreparative Enzymatic Treatments    
Tissue/Cell Culture          
Pulsed Field Sample Preparation          
Blotting      

Bio-Rad offers Certified™ Megabase Agarose, Certified Pulsed-Field Agarose, and standards for applications from FIGE separation of cosmid inserts to separation of the largest chromosomes. Choose from recombinant DNA ladders or genomic digests.
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  • Description
  • Specifications
  • Ordering
  • Kits & Reagents
  • Premixed Buffers
  • Systems
  • Documents

Description

Certified megabase agarose — With very high gel strength, high electrophoretic mobility, and high exclusion limit, Certified megabase agarose is the superior choice for CHEF and FIGE applications. The gels remain easy to handle at concentrations as low as 0.3% and allow shorter run times. The separation range is between 1 kb and 5 Mb. Low background staining also provides superior imaging of high molecular weight DNA. Certified megabase agarose has superior electrophoretic mobility, faster migration rates, faster running times, and better separation of megabase fragments than pulsed field Certified agarose.

Pulsed field Certified™ agarose — This agarose enables excellent separation and resolution of large DNA in pulsed field gel applications. The optimal separation range is 1 kb–2 Mb. Although gels cast with this agarose have longer PFGE run times than Certified megabase agarose, running conditions for this agarose are a preset selectable method of the CHEF Mapper XA system auto-algorithm.

Pulsed field standards — Standards for pulsed field applications save days of preparation time. Bio-Rad offers standards for applications from FIGE separation of cosmid inserts to separation of the largest chromosomes. The higher molecular weight standards are prepared in low-melt agarose blocks that can be cut to fit most well dimensions. Choose from recombinant DNA ladders or genomic digests.


Specifications

5 kb Ladder  
Range 4.9–120 kb
Contents Concatemers of pBR328 partially digested with EcoRI
Supplied in  Pulsed field Certified agarose
Quantity 20 µg
Concentration 0.1 µg/µl
Number of applications 20–25
8–48 kb Ladder  
Range 8.3–48.5 kb
Contents Mixed digest of phage λ
Supplied in Pulsed field Certified agarose 
Quantity 25 µg
Concentration 0.2 µg/µl
Number of applications 125
Lambda (λ) Ladder  
Range 0.05–1 Mb
Contents Concatemers of phage λ c1857 Sam7
Supplied in Pulsed field Certified agarose
Quantity 5 agarose blocks
Concentration 15 µg per insert
Number of applications  25–40
S. cerevisiae Size Marker  
Range 0.225–2.2 Mb
Contents Saccharomyces cerevisiae chromosomal DNA
Supplied in  Pulsed field Certified agarose
Quantity  5 agarose blocks
Concentration 1.75 x 108 cells/insert
Number of applications 25–40
H. wingei Size Marker  
Range 1–3.1 Mb
Contents   Hansenula wingei chromosomal DNA
Supplied in    Pulsed field Certified agarose
Quantity 5 agarose blocks 
Number  of applications 25–40
S. pombe Size Marker  
Range 3.5–5.7 Mb
Contents Schizosaccharomyces pombe chromosomal DNA
Supplied in Pulsed field Certified  agarose
Quantity    5 agarose blocks 
Concentration 2.25 x 108 cells/insert
Number of applications 25–40

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Certified Megabase Agarose, 25 g

1613108
25 g, agarose for use with CHEF and FIGE applications, certified free of inhibitors, DNases, RNases

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$105.00 
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Certified Megabase Agarose, 125 g

1613109
125 g, agarose for use with CHEF and FIGE applications, certified free of inhibitors, DNases, RNases

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$349.00 
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Certified Megabase Agarose, 500 g

1613110
500 g, agarose for use with CHEF and FIGE applications, certified free of inhibitors, DNases, RNases

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$1,082.00 
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Certified Molecular Biology Agarose, 25 g

1613100
25 g, general purpose agarose for nucleic acid electrophoresis, high gel strength, certified free of inhibitors, DNases, RNases

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$70.00 
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Certified Molecular Biology Agarose, 125 g

1613101
125 g, general purpose agarose for nucleic acid electrophoresis, high gel strength, certified free of inhibitors, DNases, RNases

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$213.00 
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Certified Molecular Biology Agarose, 500g

1613102
500 g, general purpose agarose for nucleic acid electrophoresis, high gel strength, certified free of inhibitors, DNases, RNases

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$689.00 
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Pulsed Field Certified Agarose, 100 g

1620137
100 g, agarose for separation of large DNA in pulsed field gel applications, certified free of inhibitors, DNases, RNases

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$281.00 
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Pulsed Field Certified Agarose, 500 g

1620138
500 g, agarose for separation of large DNA in pulsed field gel applications, certified free of inhibitors, DNases, RNases

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$1,238.00 
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CHEF DNA Size Marker, 0.2–2.2 Mb, S. cerevisiae Ladder

1703605
5 agarose blocks, PFGE marker, 0.225–2.2 Mb S. cerevisiae chromosomal DNA, for 25–40 plugs

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$185.00 
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CHEF DNA Size Marker, 1–3.1 Mb, H. wingei Ladder

1703667
5 agarose blocks, PFGE marker, 1–3.1 Mb, H. wingei chromosomal DNA, for 25–40 plugs

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$197.00 
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(Discontinued) CHEF DNA Size Marker

1703633
5 agarose blocks, PFGE marker, 3.5–5.7 Mb, S. pombe chromosomal DNA, for 25–40 plugs

This product is no longer available. Please contact technical support for more information on replacement product.

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CHEF DNA Size Standard, 4.9–98 kb Ladder

1703624
200 μl, 0.1 μg/μl, PFGE standard, 5 kb ladder, 4.9–120 kb, concatemers of pBR328, 20–25 applications

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$256.00 
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CHEF DNA Size Standard, 8.3–48.5 kb, Lambda Ladder

1703707
125 μl, 0.2 μg/μl, PFGE standard, 8.3–48.5 kb, mixed digests of phage lambda (λ), 125 applications

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$106.00 
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CHEF DNA Size Standard, 48.5–1,000 kb, Lambda Ladder

1703635
5 agarose blocks, PFGE standard, lambda (λ) ladder, 48.5-1000 kb in 48.5 kb increments, concatemers of λcl857Sam7 lambda DNA per block, for 25–40 plugs

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$514.00 
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Kits & Reagents

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Ethidium Bromide Solution, 10 mg/mL

1610433
10 mL ethidium bromide solution for fluorescent staining of nucleic acids, 10 mg/mL

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$46.00 
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Hazardous  

Premixed Buffers

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10x TBE (Tris/Boric Acid/EDTA) Buffer, 1 L

1610733
1 L, TBE nucleic acid electrophoresis buffer solution, pH 8.3

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$43.00 
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50x TAE (Tris/Acetic Acid/EDTA) Buffer, 1 L

1610743
1 L, TAE nucleic acid electrophoresis buffer solution, pH 8.0

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$100.00 
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50x TAE (Tris/Acetic Acid/EDTA) Buffer, 5 L

1610773
5 L cube, TAE nucleic acid electrophoresis buffer solution, pH 8.0

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$294.00 
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10x TBE (Tris/Boric Acid/EDTA) Buffer, 5 L

1610770
5 L cube, TBE nucleic acid electrophoresis buffer solution, pH 8.3

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$132.00 
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Systems

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CHEF Mapper XA Chiller System, 120 V

1703670
120 V, complete PFGE system, includes cooling module, pump, tubing, S. cerevisiae DNA size standard, 5 g pulsed field Certified agarose, 5 g pulsed field Certified megabase agarose

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CHEF-DR III Chiller System

1703700
120 V, complete variable angle PFGE system with cooling module, includes all gel casting accessories, pump, tubing, DNA standards, 2 plugs of S. cerevisiae DNA size standard, PFGE grade agarose

This product is being discontinued soon. Please contact customer service for more information.

The following replacement is recommended:

  • CHEF Mapper XA Chiller System, 120 V (1703670)
(Discontinued) CHEF-DR II Chiller System

1703725
120 V, complete PFGE system with cooling module, pump, gel casting accessories, 2 plugs S. cerevisiae DNA size standard, 5 g pulsed field Certified agarose, 5 g pulsed field Certified megabase agarose

This product is no longer available. Please contact technical support for more information on replacement product.

The following replacement is recommended:

  • CHEF Mapper XA Chiller System, 120 V (1703670)

Documents

Number Description Options
5901 Pulsed Field Gel Electrophoresis Brochure, Rev B Click to download
LIT388 Instruction Manual, CHEF DNA Size Marker, S. pombe, Rev C Click to download
LIT291 Instruction Manual, CHEF DNA Size Standards, 5 kb Ladder, Rev B Click to download
LIT538 Instruction Manual, CHEF DNA Size Marker, H. wingei, Rev B Click to download
LIT299 Instruction Manual, CHEF DNA Size Marker, S. cerevisiae, Rev E Click to download
LIT556 Instruction Manual, CHEF DNA Size Standards, 8–48 kb, Rev B Click to download
LIT298 Instruction Manual, CHEF DNA Size Standards, Lambda Ladder, Rev C Click to download
6228 Using Pulsed Field Gel Electrophoresis Standards, Rev A Click to download

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