Pulsed Field Standards and Agaroses

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Overview

Bio-Rad offers Certified™ Megabase Agarose, Certified Pulsed-Field Agarose, and standards for applications from FIGE separation of cosmid inserts to separation of the largest chromosomes. Choose from recombinant DNA ladders or genomic digests.
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Description

Description

Certified megabase agarose — With very high gel strength, high electrophoretic mobility, and high exclusion limit, Certified megabase agarose is the superior choice for CHEF and FIGE applications. The gels remain easy to handle at concentrations as low as 0.3% and allow shorter run times. The separation range is between 1 kb and 5 Mb. Low background staining also provides superior imaging of high molecular weight DNA. Certified megabase agarose has superior electrophoretic mobility, faster migration rates, faster running times, and better separation of megabase fragments than pulsed field Certified agarose.

Pulsed field Certified™ agarose — This agarose enables excellent separation and resolution of large DNA in pulsed field gel applications. The optimal separation range is 1 kb–2 Mb. Although gels cast with this agarose have longer PFGE run times than Certified megabase agarose, running conditions for this agarose are a preset selectable method of the CHEF Mapper XA system auto-algorithm.

Pulsed field standards — Standards for pulsed field applications save days of preparation time. Bio-Rad offers standards for applications from FIGE separation of cosmid inserts to separation of the largest chromosomes. The higher molecular weight standards are prepared in low-melt agarose blocks that can be cut to fit most well dimensions. Choose from recombinant DNA ladders or genomic digests.

Specifications

Specifications

5 kb Ladder
 
Range
4.9–120 kb
Contents
Concatemers of pBR328 partially digested with EcoRI
Supplied in 
Pulsed field Certified agarose
Quantity
20 µg
Concentration
0.1 µg/µl
Number of applications
20–25
8–48 kb Ladder
 
Range
8.3–48.5 kb
Contents
Mixed digest of phage λ
Supplied in
Pulsed field Certified agarose 
Quantity
25 µg
Concentration
0.2 µg/µl
Number of applications
125
Lambda (λ) Ladder
 
Range
0.05–1 Mb
Contents
Concatemers of phage λ c1857 Sam7
Supplied in
Pulsed field Certified agarose
Quantity
5 agarose blocks
Concentration
15 µg per insert
Number of applications 
25–40
S. cerevisiae Size Marker
 
Range
0.225–2.2 Mb
Contents
Saccharomyces cerevisiae chromosomal DNA
Supplied in 
Pulsed field Certified agarose
Quantity 
5 agarose blocks
Concentration
1.75 x 108 cells/insert
Number of applications
25–40
H. wingei Size Marker
 
Range
1–3.1 Mb
Contents  
Hansenula wingei chromosomal DNA
Supplied in   
Pulsed field Certified agarose
Quantity
5 agarose blocks 
Number  of applications
25–40
S. pombe Size Marker
 
Range
3.5–5.7 Mb
Contents
Schizosaccharomyces pombe chromosomal DNA
Supplied in
Pulsed field Certified  agarose
Quantity   
5 agarose blocks 
Concentration
2.25 x 108 cells/insert
Number of applications
25–40

Ordering

Ordering

items

Use the filters below to refine results!

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1613100
25 g, general purpose agarose for nucleic acid electrophoresis, high gel strength, certified free of inhibitors, DNases, RNases
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1613101
125 g, general purpose agarose for nucleic acid electrophoresis, high gel strength, certified free of inhibitors, DNases, RNases
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1613102
500 g, general purpose agarose for nucleic acid electrophoresis, high gel strength, certified free of inhibitors, DNases, RNases
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1620137
100 g, agarose for separation of large DNA in pulsed field gel applications, certified free of inhibitors, DNases, RNases
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1620138
500 g, agarose for separation of large DNA in pulsed field gel applications, certified free of inhibitors, DNases, RNases
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1703605
5 agarose blocks, PFGE marker, 0.225–2.2 Mb S. cerevisiae chromosomal DNA, for 25–40 plugs
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(Discontinued) CHEF DNA Size Marker
1703633
5 agarose blocks, PFGE marker, 3.5–5.7 Mb, S. pombe chromosomal DNA, for 25–40 plugs
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1703624
200 μl, 0.1 μg/μl, PFGE standard, 5 kb ladder, 4.9–120 kb, concatemers of pBR328, 20–25 applications
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1703707
125 μl, 0.2 μg/μl, PFGE standard, 8.3–48.5 kb, mixed digests of phage lambda (λ), 125 applications
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1703635
5 agarose blocks, PFGE standard, lambda (λ) ladder, 48.5-1000 kb in 48.5 kb increments, concatemers of λcl857Sam7 lambda DNA per block, for 25–40 plugs

Kits & Reagents

Kits & Reagents

Premixed Buffers

Premixed Buffers

Systems

Systems

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1703670
120 V, complete PFGE system, includes cooling module, pump, tubing, S. cerevisiae DNA size standard, 5 g pulsed field Certified agarose, 5 g pulsed field Certified megabase agarose
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1703700
120 V, complete variable angle PFGE system with cooling module, includes all gel casting accessories, pump, tubing, DNA standards, 2 plugs of S. cerevisiae DNA size standard, PFGE grade agarose
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1703725
120 V, complete PFGE system with cooling module, pump, gel casting accessories, 2 plugs S. cerevisiae DNA size standard, 5 g pulsed field Certified agarose, 5 g pulsed field Certified megabase agarose

Documents

Documents

Number Description Options
5901
Pulsed Field Gel Electrophoresis Brochure, Rev B
LIT388
Instruction Manual, CHEF DNA Size Marker, S. pombe, Rev C
LIT291
Instruction Manual, CHEF DNA Size Standards, 5 kb Ladder, Rev B
LIT538
Instruction Manual, CHEF DNA Size Marker, H. wingei, Rev B
LIT299
Instruction Manual, CHEF DNA Size Marker, S. cerevisiae, Rev E
LIT556
Instruction Manual, CHEF DNA Size Standards, 8–48 kb, Rev B
LIT298
Instruction Manual, CHEF DNA Size Standards, Lambda Ladder, Rev C
6228
Using Pulsed Field Gel Electrophoresis Standards, Rev A