Restriction Enzymes

Engage students in techniques used with DNA evidence in forensics to assist in criminal cases, including restriction enzyme digestion and mapping restriction sites on genes.

	Analysis of Precut Lambda DNA Kit	Restriction Digestion and Analysis of Lambda DNA Kit	Forensic DNA Fingerprinting Kit
Overview	The analysis of precut lambda DNA kit demonstrates the principles, results, and analysis of restriction digestion without the extra time needed to perform the digestion.	Students use three different restriction enzymes to digest genomic DNA from lambda bacteriophage. With this kit, students will learn electrophoretic techniques, generate standard curves, and analyze migration distances to determine DNA sizes.	Become a crime scene investigator by using real DNA and find out “Who done it?”. Students study restriction enzymes, electrophoretic techniques, use digest maps of lambda bacteriophage genomes to design novel plasmids.
Ideal For	Teachers who want to teach the technique of DNA gel electrophoresis with a minimal amount of teacher preparation and limited classroom time	Teachers who want to teach plasmid mapping, perform a restriction digest, and want to perform DNA gel electrophoresis	Teachers who want an engaging forensics storyline while still teaching the essential skills of restriction digestions, plasmid mapping and DNA gel electrophoresis
AP Alignment	Map of AP-Aligned Bio-Rad Kits with Essential Knowledge Map of AP-Aligned Bio-Rad Kits with Investigative Laboratories Map of AP-Aligned Bio-Rad Kits with Learning Objectives Inquiry Based Learning Poster
Pedagogical Support	Fast Protein and Nucleic Acid Electrophoresis Flier	Fast Protein and Nucleic Acid Electrophoresis Flier	20 Questions to Master Inquiry - Forensic DNA Fingerprinting Fast Protein and Nucleic Acid Electrophoresis Flier
Class Sessions	Two 45 minute classes	Two 45 minute classes	Two 45 minute classes
Inquiry Type	Structured	Structured	Structured
Required Equipment	Horizontal Gel Electrophoresis Chambers Adjustable Micropipets Power Supplies Gel Staining Trays	Horizontal Gel Electrophoresis Chambers Adjustable Micropipets Power Supplies Gel Staining Trays	Horizontal Gel Electrophoresis Chambers Adjustable Micropipets Power Supplies Gel Staining Trays

Teachers who bought these items, also bought:

PV92 PCR Informatics Kit

In this activity, students use real-world forensic techniques to extract DNA from their hair follicles or cheek cells, and then use PCR amplification and electrophoresis to fingerprint their own DNA.
pGLO Transformation and Inquiry Kit for AP Biology

Developed with master AP Biology teachers, this 5-in-1 inquiry kit solution for AP Big Ideas 1 and 3 will give you the tools for multiple levels of inquiry.
Comparative Proteomics Kit I: Protein Profiler Module

This module allows students to employ protein electrophoresis, the most widely used technique in life science research, to study protein structure and function.