Analysis of Precut Lambda DNA Kit

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Overview

This activity aids teachers in providing in-depth explanations of how restriction enzymes cut DNA and how electrophoresis can be used to separate and visualize
DNA fragments.

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Description

Description

DNA gels

Run your DNA gels in 10 minutes!

Find Out How

Electrophoretic techniques that distinguish DNA fragments by size are essential in forensics and in the mapping of restriction sites within genes. The Analysis of Precut Lambda DNA Kit demonstrates basic procedures and principles of DNA gel electrophoresis, including agarose gel casting, sample loading, size-based separation of DNA fragments, DNA staining, and graphic analysis. This activity provides in-depth explanations about how restriction enzymes cut DNA and how electrophoresis can be used to separate and visualize DNA fragments.

Features and Benefits

  • Aligns with AP Biology Lab 9

With this kit, students are able to:

  • Study DNA restriction enzyme function
  • Use electrophoresis to separate DNA fragments
  • Construct standard curves from their data and make precise determinations of DNA fragment sizes

Applications and Uses

By visualizing the effect of three restriction enzymes on four identical samples of double-stranded lambda virus DNA, students learn that different restriction enzymes recognize and cut different DNA sequences. Optional extension exercises guide students through the procedure of DNA fragment size determination by constructing a standard curve using their own gel data. Students can then use the standard curve to determine the sizes of the various DNA fragments in their samples.

More Information

  • Two–session laboratory activity, 45 min per session
  • Provides sufficient materials for eight student workstations, up to four students per workstation

Specifications

Specifications

Kit contains sufficient materials for 8 student workstations (2–4 students per workstation)
 
PstI lambda DNA digest
1
EcoRI lambda DNA digest
1
Lambda DNA, uncut
1
DNA size standard (HindIII lambda DNA digest)
1
Sample loading buffer, 5x, 1 ml
1
Agarose powder, 5 g
1
Electrophoresis buffer, 50x TAE, 100 ml
1
Fast Blast™ DNA stain, 500x, 100 ml
1
Colored microcentrifuge tubes, 2.0 ml
60
Curriculum, including teacher's guide, student manual, and graphic quick guide
1

Ordering

Ordering

items

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1660001EDU
DNA gel electrophoresis classroom study kit, includes DNA standard and digests, buffers, agarose, Fast Blast DNA stain, test tubes, curriculum, and more, for 32 students; education use only

Refills

Refills

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1660011EDU
Consumables refill kit for use with Analysis of Precut Lambda DNA Kit (1660001EDU), includes DNA size standard, PstI lambda DNA digest, EcoRI lambda DNA digest, uncut lambda DNA, and sample loading buffer; educational use only
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(Discontinued) Fast Blast DNA Stain
1660420EDU
100 ml, nontoxic 500x concentrate, for deep blue DNA staining of agarose and polyacrylamide gels; education use only

Documents

Documents

Number Description Options
4006101
Instruction Manual, Analysis of Precut Lambda DNA Kit, Biotechnology Explorer, Rev E
6672
Map of AP-Aligned Bio-Rad Kits with Essential Knowledge
6673
Map of AP-Aligned Bio-Rad Kits with Investigative Laboratories
6605
Map of AP-Aligned Bio-Rad Kits with Learning Objectives
5396
Fast Gel Protocol — Easily Run Your DNA Gels in Under 10 Minutes — Bio-Rad Explorer, Ver D
3054
Analysis of Precut Lambda DNA Kit, Biotechnology Explorer, Quick Guide
5891
Refresh Kit Components — Large Class/Multiple Class Preparation Guide
5914
DNA Staining Using Fast Blast™ DNA Stain or SYBR® Safe Stain and Digital Analysis of Gel Images Using Logger Pro Software
6602
UView™ 6x Loading Dye and Stain Guide, Bio-Rad Explorer™
6916
Fast Protein and Nucleic Acid Electrophoresis Flier