Analysis of Precut Lambda DNA Kit

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Analysis of Precut Lambda DNA Kit

Overview

Lab Preparation Checklist
Kit contains sufficient materials for 8 student workstations (2–4 students per workstation)
PstI lambda DNA digest 1
EcoRI lambda DNA digest 1
Lambda DNA, uncut 1
DNA size standard (HindIII lambda DNA digest) 1
Sample loading buffer, 5x, 1 ml 1
Agarose powder, 5 g 1
Electrophoresis buffer, 50x TAE, 100 ml 1
Fast Blast™ DNA stain, 500x, 100 ml 1
Colored microcentrifuge tubes, 2.0 ml 60
Curriculum including Teacher's Guide, Student Manual, and graphic Quick Guide 1
Required Accessories Not Included in Kit:
Horizontal gel electorphoresis chambers 4–8
Adjustable micropipets
    2–20 µl 4–8
    20–200 µl 1
Pipet tips, 2–200 µl, BR-35 1 bag
Power supplies 2–4
Recommended (Optional) Accessories:
Gel Staining trays  
Tube racks  
Rocking platform  
Gel support film  
Microwave oven  
Analysis of Precut Lambda DNA Flowchart
Analysis of Precut Lambda DNA Flowchart
Purchasing Guide Table

Reduce. Reuse. Refresh.

Refresh kit components, reduce packaging waste, reuse components, and refresh your kits, and you’ll save storage space by purchasing individual items. Bio-Rad now has many individual components for Biotechnology Explorer™ kits available for purchase.


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This activity aids teachers in providing in-depth explanations of how restriction enzymes cut DNA and how electrophoresis can be used to separate and visualize
DNA fragments.


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Ordering Information


  • Description
  • Specifications
  • Ordering
  • Refills
  • Documents

Description

DNA gels

Run your DNA gels in 10 minutes!

Find Out How

Electrophoretic techniques that distinguish DNA fragments by size are essential in forensics and in the mapping of restriction sites within genes. The Analysis of Precut Lambda DNA Kit demonstrates basic procedures and principles of DNA gel electrophoresis, including agarose gel casting, sample loading, size-based separation of DNA fragments, DNA staining, and graphic analysis. This activity provides in-depth explanations about how restriction enzymes cut DNA and how electrophoresis can be used to separate and visualize DNA fragments.

Features and Benefits

  • Aligns with AP Biology Lab 9

With this kit, students are able to:

  • Study DNA restriction enzyme function
  • Use electrophoresis to separate DNA fragments
  • Construct standard curves from their data and make precise determinations of DNA fragment sizes

Applications and Uses

By visualizing the effect of three restriction enzymes on four identical samples of double-stranded lambda virus DNA, students learn that different restriction enzymes recognize and cut different DNA sequences. Optional extension exercises guide students through the procedure of DNA fragment size determination by constructing a standard curve using their own gel data. Students can then use the standard curve to determine the sizes of the various DNA fragments in their samples.

More Information

  • Two–session laboratory activity, 45 min per session
  • Provides sufficient materials for eight student workstations, up to four students per workstation

Specifications

Kit contains sufficient materials for 8 student workstations (2–4 students per workstation)  
PstI lambda DNA digest 1
EcoRI lambda DNA digest 1
Lambda DNA, uncut 1
DNA size standard (HindIII lambda DNA digest) 1
Sample loading buffer, 5x, 1 ml 1
Agarose powder, 5 g 1
Electrophoresis buffer, 50x TAE, 100 ml 1
Fast Blast™ DNA stain, 500x, 100 ml 1
Colored microcentrifuge tubes, 2.0 ml 60
Curriculum, including Teacher's Guide, Student Manual, and graphic Quick Guide 1

Ordering

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list price. If the problem persists, please call customer service at or send an
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Analysis of Precut Lambda DNA Kit

1660001EDU
DNA gel electrophoresis classroom study kit, includes DNA standard and digests, buffers, agarose, Fast Blast DNA stain, test tubes, curriculum, and more, for 32 students; education use only

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$123.75 
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$99.00  
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Refills

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If you are placing an order, you may proceed with your order; the account price will be applied if it is lower than the
list price. If the problem persists, please call customer service at or send an
email to

Lambda DNA Kit Refill

1660011EDU
Consumables refill kit for use with Analysis of Precut Lambda DNA Kit (1660001EDU), includes DNA size standard, PstI lambda DNA digest, EcoRI lambda DNA digest, uncut lambda DNA, and sample loading buffer; educational use only

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$103.75 
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$83.00  
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Fast Blast DNA Stain

1660420EDU
100 ml, nontoxic 500x concentrate, for deep blue DNA staining of agarose and polyacrylamide gels; education use only

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$31.25 
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$25.00  
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Jellyfish Foam Floating Racks

1660479EDU
Pkg of 8, microcentrifuge tube rack, holds 12 tubes; education use only

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$40.00 
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$32.00  
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Gel Staining Trays

1660477EDU
Pkg of 4; education use only

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$22.50 
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$18.00  
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Documents

Number Description Options
4006101 Instruction Manual, Analysis of Precut Lambda DNA Kit, Biotechnology Explorer, Rev E Please login to download this document
6672 Map of AP-Aligned Bio-Rad Kits with Essential Knowledge Click to download
6673 Map of AP-Aligned Bio-Rad Kits with Investigative Laboratories Click to download
6605 Map of AP-Aligned Bio-Rad Kits with Learning Objectives Click to download
5396 Fast Gel Protocol — Easily Run Your DNA Gels in Under 10 Minutes — Bio-Rad Explorer, Ver D Click to download
3054 Analysis of Precut Lambda DNA Kit, Biotechnology Explorer, Quick Guide Please login to download this document
5891 Refresh Kit Components — Large Class/Multiple Class Preparation Guide Click to download
5872 Bio-Rad Explorer™ Refresh Kit Components Purchasing Guide Click to download
5914 DNA Staining Using Fast Blast™ DNA Stain or SYBR® Safe Stain and Digital Analysis of Gel Images Using Logger Pro Software Click to download
6602 UView™ 6x Loading Dye and Stain Guide, Bio-Rad Explorer™ Click to download
6916 Fast Protein and Nucleic Acid Electrophoresis Flier Click to download

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