Analysis of Precut Lambda DNA Kit

Analysis of Precut Lambda DNA Kit

This activity aids teachers in providing in-depth explanations of how restriction enzymes cut DNA and how electrophoresis can be used to separate and visualize
DNA fragments.

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Overview

DNA gels

Run your DNA gels in 10 minutes!

Find Out How

Electrophoretic techniques that distinguish DNA fragments by size are essential in forensics and in the mapping of restriction sites within genes. The Analysis of Precut Lambda DNA Kit demonstrates basic procedures and principles of DNA gel electrophoresis, including agarose gel casting, sample loading, size-based separation of DNA fragments, DNA staining, and graphic analysis. This activity provides in-depth explanations about how restriction enzymes cut DNA and how electrophoresis can be used to separate and visualize DNA fragments.

Features and Benefits

  • Aligns with AP Biology Lab 9

With this kit, students are able to:

  • Study DNA restriction enzyme function
  • Use electrophoresis to separate DNA fragments
  • Construct standard curves from their data and make precise determinations of DNA fragment sizes

Applications and Uses

By visualizing the effect of three restriction enzymes on four identical samples of double-stranded lambda virus DNA, students learn that different restriction enzymes recognize and cut different DNA sequences. Optional extension exercises guide students through the procedure of DNA fragment size determination by constructing a standard curve using their own gel data. Students can then use the standard curve to determine the sizes of the various DNA fragments in their samples.

More Information

  • Two–session laboratory activity, 45 min per session
  • Provides sufficient materials for eight student workstations, up to four students per workstation

Specifications

# Workstations
8
# Students per workstation
4
# Lab sessions (45-minute labs)
2
Shipping temperature
Ambient
Storage temperature (upon delivery)
4°C as indicated

Kit Contents

Lambda DNA Kit Refill
PstI lambda DNA digest
100 µl
EcoRI lambda DNA digest
100 µl
Lambda DNA, uncut
100 µl
DNA size standard (HindIII lambda DNA digest)
100 µl
Sample loading buffer (5x)
1 ml
Other Reagents and Consumables
Agarose
5 g
TAE electrophoresis buffer (50x)
100 ml
Fast Blast DNA stain (500x)
100 ml
Microcentrifuge tubes, 2.0 ml, multicolor
60
Instructions
Instructor and Student Guide
Fast Gel Protocol

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items

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BE_PreCut_DNA_KitBox--SKU – Thumbnail.jpg
1660001EDU
DNA gel electrophoresis classroom study kit, includes DNA standard and digests, buffers, agarose, Fast Blast DNA stain, test tubes, curriculum, and more, for 32 students; education use only

Refills

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1660011EDU-tn.jpg
1660011EDU
Consumables refill kit for use with Analysis of Precut Lambda DNA Kit (1660001EDU), includes DNA size standard, PstI lambda DNA digest, EcoRI lambda DNA digest, uncut lambda DNA, and sample loading buffer; educational use only
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166-0420EDU_tn.jpg
(Discontinued) Fast Blast DNA Stain
1660420EDU
100 ml, nontoxic 500x concentrate, for deep blue DNA staining of agarose and polyacrylamide gels; education use only

Documents

Number Description
4006101
Instruction Manual, Analysis of Precut Lambda DNA Kit, Biotechnology Explorer, Rev E
3054
Analysis of Precut Lambda DNA Kit, Biotechnology Explorer, Quick Guide