Restriction Digestion and Analysis of Lambda DNA Kit

Restriction Digestion and Analysis of Lambda DNA Kit

This DNA analysis kit demonstrates the procedures and principles of DNA restriction digestion. Digestion of phage lambda DNA is followed by agarose gel electrophoresis. Students get hands-on experience with gel casting, sample loading

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Overview

DNA gels

Run your DNA gels in 10 minutes!

Find Out How

Electrophoretic techniques that distinguish DNA fragments by size are essential in forensics and in the mapping of restriction sites within genes. With the Restriction Digestion and Analysis of Lambda DNA Kit, students use three different restriction enzymes to digest genomic DNA from lambda bacteriophage. By visualizing the effects of three different enzymes on identical samples of double-stranded DNA, students learn that different restriction enzymes recognize and cut different DNA sequences.

Features and Benefits

  • Aligns with AP Lab 6
  • Teaches about using genetic engineering and restriction enzymes as microbiology tools

With this kit, students are able to:

  • Use agarose gel electrophoresis to study digestion patterns
  • Generate standard curves from series of DNA size standards
  • Analyze migration distances to determine DNA fragment sizes

Students learn the procedures and principles of restriction digestions and DNA gel electrophoresis, including:

  • Agarose gel casting
  • Sample loading
  • Size-based separation of DNA fragments
  • DNA staining
  • Graphic analysis

How It Works

The lambda genome has approximately 48,000 base pairs. Each restriction enzyme used in this kit cuts the lambda DNA several times, generating distinct sets of DNA restriction fragments of different sizes. The three different sets of DNA fragments that result from the enzymatic digestion are separated by agarose gel electrophoresis and visualized using Bio-Rad's safe Fast Blast™ DNA stain. Banding patterns from each sample are then compared to each other and to a DNA size standard. Students use their electrophoresis results to construct standard curves and determine the precise DNA fragment sizes generated by the different restriction enzymes.

More Information

  • Two-session laboratory activity, 45 min per session
  • Provides sufficient materials for eight student workstations, up to four students per workstation

Specifications

# Workstations
8
# Students per workstation
4
# Lab sessions (45-minute labs)
2
Shipping temperature
Ambient and dry ice
Storage temperature (upon delivery)
Ambient or –20°C as indicated

Kit Contents

Restriction Digestion Kit TS Refill Package
Lambda DNA, uncut
100 µl
HindIII enzyme
40 µl
PstI enzyme
40 µl
EcoRI enzyme
40 µl
Restriction buffer (2x)
500 µl
DNA size standard (HindIII lambda DNA digest)
100 µl
Sample loading buffer (5x)
1 ml
Restriction Digestion Kit RT Refill Package
Agarose
5 g
TAE electrophoresis buffer (50x)
100 ml
Fast Blast DNA stain (500x)
100 ml
Microcentrifuge tubes, 2.0 ml, multicolor
60
Foam floats
8
Instructions
Instructor and Student Guide
Fast Gel Protocol

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1660002EDU
DNA restriction analysis classroom study kit, includes DNA, restriction enzymes, buffers, agarose, Fast Blast DNA stain, curriculum, and more, for 32 students; education use only

Refills

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1660012EDU
Temperature sensitive refill for use with Restriction Digestion and Analysis of Lambda DNA Kit (1660002EDU), includes enzymes, buffer, DNA samples, DNA size standard, sample loading buffer; education use only
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1660022EDU
Room temperature refill for use with Restriction Digestion and Analysis of Lambda DNA Kit (1660002EDU); includes agarose powder, 50x TAE electrophoresis buffer, Fast Blast DNA stain, micro test tubes, foam test tube holders, curriculum; education use only

Documents

Number Description
4006102
Instruction Manual, Restriction Digestion and Analysis of Lambda DNA Kit, Bio-Rad Explorer™
3055
Bio-Rad Explorer™ Restriction Digestion and Analysis of Lambda DNA Kit Quick Guide