Restriction Digestion and Analysis of Lambda DNA Kit

Image
lsr_restrction_digestion_analysis_lambda_dna_kit.jpg

Overview

This DNA analysis kit demonstrates the procedures and principles of DNA restriction digestion. Digestion of phage lambda DNA is followed by agarose gel electrophoresis. Students get hands-on experience with gel casting, sample loading

Read full description Ordering Information

Description

Description

DNA gels

Run your DNA gels in 10 minutes!

Find Out How

Electrophoretic techniques that distinguish DNA fragments by size are essential in forensics and in the mapping of restriction sites within genes. With the Restriction Digestion and Analysis of Lambda DNA Kit, students use three different restriction enzymes to digest genomic DNA from lambda bacteriophage. By visualizing the effects of three different enzymes on identical samples of double-stranded DNA, students learn that different restriction enzymes recognize and cut different DNA sequences.

Features and Benefits

  • Aligns with AP Lab 6
  • Teaches about using genetic engineering and restriction enzymes as microbiology tools

With this kit, students are able to:

  • Use agarose gel electrophoresis to study digestion patterns
  • Generate standard curves from series of DNA size standards
  • Analyze migration distances to determine DNA fragment sizes

Students learn the procedures and principles of restriction digestions and DNA gel electrophoresis, including:

  • Agarose gel casting
  • Sample loading
  • Size-based separation of DNA fragments
  • DNA staining
  • Graphic analysis

How It Works

The lambda genome has approximately 48,000 base pairs. Each restriction enzyme used in this kit cuts the lambda DNA several times, generating distinct sets of DNA restriction fragments of different sizes. The three different sets of DNA fragments that result from the enzymatic digestion are separated by agarose gel electrophoresis and visualized using Bio-Rad's safe Fast Blast™ DNA stain. Banding patterns from each sample are then compared to each other and to a DNA size standard. Students use their electrophoresis results to construct standard curves and determine the precise DNA fragment sizes generated by the different restriction enzymes.

More Information

  • Two-session laboratory activity, 45 min per session
  • Provides sufficient materials for eight student workstations, up to four students per workstation

Specifications

Specifications

Kit contains sufficient materials for 8 student workstations (2–4 students per workstation)
 
HindIII restriction enzyme
1
Pstl restriction enzyme
1
EcoRI restriction enzyme
1
Restriction buffer
1
Lambda DNA, uncut
1
DNA size standard (HindIII lambda DNA digest)
1
Sample loading buffer, 5x, 1 ml
1
Agarose powder, 5g
1
Electrophoresis buffer, 50x TAE, 100 ml
1
Fast Blast™ DNA stain, 500x, 100 ml
1
Colored microcentrifuge tubes, 2.0 ml
60
Foam floats
8
Curriculum, including teacher's guide, student manual, and graphic quick guide
1

Ordering

Ordering

items

Use the filters below to refine results!

166-0002EDU_tn.jpg
1660002EDU
DNA restriction analysis classroom study kit, includes DNA, restriction enzymes, buffers, agarose, Fast Blast DNA stain, curriculum, and more, for 32 students; education use only

Refills

Refills

166-0012EDU_tn.jpg
1660012EDU
Temperature sensitive refill for use with Restriction Digestion and Analysis of Lambda DNA Kit (1660002EDU), includes enzymes, buffer, DNA samples, DNA size standard, sample loading buffer; education use only
166-0022EDU_tn.jpg
1660022EDU
Room temperature refill for use with Restriction Digestion and Analysis of Lambda DNA Kit (1660002EDU); includes agarose powder, 50x TAE electrophoresis buffer, Fast Blast DNA stain, micro test tubes, foam test tube holders, curriculum; education use only

Documents

Documents

Number Description Options
4006102
Instruction Manual, Restriction Digestion and Analysis of Lambda DNA Kit, Bio-Rad Explorer™
3055
Bio-Rad Explorer™ Restriction Digestion and Analysis of Lambda DNA Kit Quick Guide
6672
Map of AP-Aligned Bio-Rad Kits with Essential Knowledge
6673
Map of AP-Aligned Bio-Rad Kits with Investigative Laboratories
6605
Map of AP-Aligned Bio-Rad Kits with Learning Objectives
5396
Fast Gel Protocol — Easily Run Your DNA Gels in Under 10 Minutes — Bio-Rad Explorer, Ver D
5891
Refresh Kit Components — Large Class/Multiple Class Preparation Guide
5914
DNA Staining Using Fast Blast™ DNA Stain or SYBR® Safe Stain and Digital Analysis of Gel Images Using Logger Pro Software
6602
UView™ 6x Loading Dye and Stain Guide, Bio-Rad Explorer™
6916
Fast Protein and Nucleic Acid Electrophoresis Flier