PV92 PCR Informatics Kit

Image
lsr_pv92pcr_informatics_kit.jpg

Description

Description

DNA gels

Run your DNA gels in 10 minutes!

Find Out How

With the PV92 PCR Informatics Kit, your students use real-world forensic techniques to extract DNA from their hair follicles or cheek cells, and then use PCR amplification and electrophoresis to fingerprint their own DNA at a specific genetic locus. Using their own results, students test the Hardy-Weinberg equilibrium theory within their classroom population, then go online to compare their classroom results to genetic data of populations worldwide.

Features and Benefits

  • Aligns with AP Biology Lab 8
  • Introduces PCR and applies it to population genetics

With this kit, students are able to:

  • Directly measure human diversity at the molecular level
  • Extract and amplify genomic DNA from their own samples
  • Compare results to online data

Background Information

In this activity, students hunt for a specific Alu repeat (a 300 base pair repetitive sequence of DNA) within a specific region called PV92 on chromosome 16. Over evolutionary time, up to 1 million copies of the Alu repeat have become randomly inserted throughout the human genome. Some of us carry an Alu insertion and some of us do not. Such variations among individuals' genotypes are inherited — and are the raw material of genetic diversity and evolution. These subtle variations in our DNA are evidence of our ancestry, and form the basis of personal identification via DNA fingerprinting.

More Information

  • Three-session laboratory activity, 45 min per session
  • Provides sufficient materials for eight student workstations, up to four students per workstation

Specifications

Specifications

Kit contains sufficient materials for 8 student workstations (2–4 students per workstation)
 
Positive controls: homozygous (+/+), homozygous (-/-), heterozygous (+/-)
1
PCR master mix (Taq DNA polymerase dNTPs, buffer)
1
PCR primers
1
DNA molecular mass ruler
1
InstaGene™ DNA extraction matrix, 20 ml
1
Orange G loading dye, 1 ml
1
Micro test tubes, 1.5 ml
60
PCR tubes, 0.2 ml
50
Screwcap tubes, 1.5 ml
50
Capless PCR tube adaptors, 1.5 ml
50
Agarose powder, 5 g
1
Electrophoresis buffer, 50x TAE, 100 ml
1
Fast Blast™ DNA stain, 500x, 100 ml
1
Curriculum, including teacher's guide, student manual, and graphic quick guide
1

Ordering

Ordering

items

Use the filters below to refine results!

166-2100EDU_tn.jpg
1662100EDU
PCR classroom study kit, includes positive controls, PCR master mix, primers, extraction matrix, Fast Blast DNA stain, test tubes/holders, curriculum, and more, for 32 students. PV92 PCR Kit TS (1662119EDU) and RT (1662139EDU) Refill Packs are available; education use only

Refills

Refills

166-2119EDU_tn.jpg
1662119EDU
Consumables refill kit for use with PV92 PCR Informatics Kit (1662100EDU), contains PCR primers, positive controls, Orange G loading dye, DNA molecular mass ruler, PCR master mix, buffer, DNA polymerase; education use only
166-2139EDU_tn.jpg
1662139EDU
Consumables refill kit for use with PV92 PCR Informatics kit (1662100EDU), contains InstaGene matrix, Fast Blast DNA stain, agarose, 50x TAE; shelf-stable; education use only
166-2003EDU_tn.jpg
(Discontinued) Protease
1662003EDU
1.3 ml, reagent for use with PV92 PCR Informatics kit (1662100EDU) for extracting DNA from hair follicles; education use only
166-5009EDU_tn.jpg
(Discontinued) Master Mix For PCR
1665009EDU
90 units, 1.2 ml includes 0.075 units per µl Taq DNA polymerase, reaction buffer, 4 mM MgCl2, 0.4 mM of each dNTP (dATP, dCTP, dGTP, dTTP); education use only
166-0479EDU_tn.jpg
1660479EDU
Pkg of 8, microcentrifuge tube rack, holds 12 tubes; education use only
166-0477EDU_tn.jpg
(Discontinued) Gel Staining Trays
1660477EDU
Pkg of 4; education use only

Documents

Documents

Number Description Options
6672
Map of AP-Aligned Bio-Rad Kits with Essential Knowledge
6673
Map of AP-Aligned Bio-Rad Kits with Investigative Laboratories
6605
Map of AP-Aligned Bio-Rad Kits with Learning Objectives
5396
Fast Gel Protocol — Easily Run Your DNA Gels in Under 10 Minutes — Bio-Rad Explorer, Ver D
4110053
Getting the Most out of the PV92 PCR Informatics Kit, Biotechnology Explorer, Rev C
4110052
Instruction Manual, Chromosome 16: PV92 PCR Informatics Kit, Biotechnology Explorer, Rev F
3058
Instruction Manual, PV92 PCR Informatics Kit Quick Guide, Biotechnology Explorer, Rev B
5891
Refresh Kit Components — Large Class/Multiple Class Preparation Guide
6602
UView™ 6x Loading Dye and Stain Guide, Bio-Rad Explorer™