PV92 PCR Informatics Kit

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PV92 PCR Informatics Kit

Overview

PV92 PCR Informatics Kit Flowchart
PV92 PCR Informatics Flowchart
Polymerase Chain Reaction
Purchasing Guide Table

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Compare DNA Samples via Electrophoresis
PV92 PCR Informatics Gel

Visualize and compare DNA samples as part of the PV92 PCR Informatics kit workflow.

Large Class Preparation Guide

Learn tips and techniques for preparing agar plates and agarose gels in large quantities.


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Complete Purchasing Guide

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PowerPoint Presentation for Workshops and for Teaching

PV92 PCR Informatics Kit

This PowerPoint presentation is intended for educational use only. The presentation provides educators with background on the PV92 PCR Informatics Kit and includes instructions for using the kit.

PV92 PCR Informatics Kit Download PowerPoint presentation
PV92 PCR Informatics Kit
PowerPoint Presentation for Workshops and for Teaching

PV92 PCR Informatics Kit

This PowerPoint presentation is intended for educational use only. The presentation provides educators with background on the PV92 PCR Informatics Kit and includes instructions for using the kit.

PV92 PCR Informatics Kit Download PowerPoint presentation
PV92 PCR Informatics Kit

In this activity, students use real-world forensic techniques to extract DNA from their hair follicles or cheek cells, and then use PCR amplification and electrophoresis to fingerprint their own DNA.
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  • Description
  • Specifications
  • Ordering
  • Refills
  • Documents

Description

DNA gels

Run your DNA gels in 10 minutes!

Find Out How

With the PV92 PCR Informatics Kit, your students use real-world forensic techniques to extract DNA from their hair follicles or cheek cells, and then use PCR amplification and electrophoresis to fingerprint their own DNA at a specific genetic locus. Using their own results, students test the Hardy-Weinberg equilibrium theory within their classroom population, then go online to compare their classroom results to genetic data of populations worldwide.

Features and Benefits

  • Aligns with AP Biology Lab 8
  • Introduces PCR and applies it to population genetics

With this kit, students are able to:

  • Directly measure human diversity at the molecular level
  • Extract and amplify genomic DNA from their own samples
  • Compare results to online data

Background Information

In this activity, students hunt for a specific Alu repeat (a 300 base pair repetitive sequence of DNA) within a specific region called PV92 on chromosome 16. Over evolutionary time, up to 1 million copies of the Alu repeat have become randomly inserted throughout the human genome. Some of us carry an Alu insertion and some of us do not. Such variations among individuals' genotypes are inherited — and are the raw material of genetic diversity and evolution. These subtle variations in our DNA are evidence of our ancestry, and form the basis of personal identification via DNA fingerprinting.

More Information

  • Three-session laboratory activity, 45 min per session
  • Provides sufficient materials for eight student workstations, up to four students per workstation

Specifications

Kit contains sufficient materials for 8 student workstations (2–4 students per workstation)  
Positive controls: homozygous (+/+), homozygous (-/-), heterozygous (+/-) 1
PCR master mix (Taq DNA polymerase dNTPs, buffer) 1
PCR primers 1
DNA molecular mass ruler 1
InstaGene™ DNA Extraction Matrix, 20 ml 1
Orange G loading dye, 1 ml 1
Micro test tubes, 1.5 ml 60
PCR tubes, 0.2 ml 50
Screwcap tubes, 1.5 ml 50
Capless PCR tube adaptors, 1.5 ml 50
Agarose powder, 5 g 1
Electrophoresis buffer, 50x TAE, 100 ml 1
Fast Blast™ DNA Stain, 500x, 100 ml 1
Curriculum, including Teacher's Guide, Student Manual, and graphic Quick Guide 1

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PV92 PCR Informatics Kit

1662100EDU
PCR classroom study kit, includes positive controls, PCR master mix, primers, extraction matrix, Fast Blast DNA stain, test tubes/holders, curriculum, and more, for 32 students. PV92 PCR Kit TS (1662119EDU) and RT (1662139EDU) Refill Packs are available; education use only

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$262.50 
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Refills

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If you are placing an order, you may proceed with your order; the account price will be applied if it is lower than the
list price. If the problem persists, please call customer service at or send an
email to

PV92 PCR Kit TS Refill Pack

1662119EDU
Consumables refill kit for use with PV92 PCR Informatics Kit (1662100EDU), contains PCR primers, positive controls, Orange G loading dye, DNA molecular mass ruler, PCR master mix, buffer, DNA polymerase; education use only

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$136.25 
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$109.00  
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PV92 PCR Kit RT Refill Pack

1662139EDU
Consumables refill kit for use with PV92 PCR Informatics kit (1662100EDU), contains InstaGene matrix, Fast Blast DNA stain, agarose, 50x TAE; shelf-stable; education use only

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$95.00 
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$76.00  
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Protease

1662003EDU
1.3 ml, reagent for use with PV92 PCR Informatics kit (1662100EDU) for extracting DNA from hair follicles; education use only

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$45.00 
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$36.00  
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Master Mix For PCR

1665009EDU
90 units, 1.2 ml includes 0.075 units per µl Taq DNA polymerase, reaction buffer, 4 mM MgCl2, 0.4 mM of each dNTP (dATP, dCTP, dGTP, dTTP); education use only

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$92.50 
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Jellyfish Foam Floating Racks

1660479EDU
Pkg of 8, microcentrifuge tube rack, holds 12 tubes; education use only

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$40.00 
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$32.00  
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Gel Staining Trays

1660477EDU
Pkg of 4; education use only

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$22.50 
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Documents

Number Description Options
6672 Map of AP-Aligned Bio-Rad Kits with Essential Knowledge Click to download
6673 Map of AP-Aligned Bio-Rad Kits with Investigative Laboratories Click to download
6605 Map of AP-Aligned Bio-Rad Kits with Learning Objectives Click to download
5396 Fast Gel Protocol — Easily Run Your DNA Gels in Under 10 Minutes — Bio-Rad Explorer, Ver D Click to download
4110053 Getting the Most out of the PV92 PCR Informatics Kit, Biotechnology Explorer, Rev C Click to download
4110052 Instruction Manual, Chromosome 16: PV92 PCR Informatics Kit, Biotechnology Explorer, Rev F Please login to download this document
3058 Instruction Manual, PV92 PCR Informatics Kit Quick Guide, Biotechnology Explorer, Rev B Click to download
5891 Refresh Kit Components — Large Class/Multiple Class Preparation Guide Click to download
5872 Bio-Rad Explorer™ Refresh Kit Components Purchasing Guide Click to download
6602 UView™ 6x Loading Dye and Stain Guide, Bio-Rad Explorer™ Click to download

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