Forensic DNA Fingerprinting Kit

Forensic DNA Fingerprinting Kit

Illustrate the principles of forensic DNA testing. Using real DNA as evidence, your students play the role of crime scene investigators to find out "Who done it".

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Overview

DNA gels

Run your DNA gels in 10 minutes!

Find Out How

DNA evidence assists in criminal, missing persons, mass disaster, and paternity cases. It can be used to identify a perpetrator or exonerate the innocent. The Forensic DNA Fingerprinting kit allows your students to use real DNA as evidence, as they play the role of crime scene investigator to figure out for themselves "Who done it?"

Features and Benefits

  • Aligns with AP Biology Big Idea 3, Lab 9
  • Two–session laboratory activity, 45 min per session
  • Provides sufficient materials for eight student workstations, up to four students per workstation

With this kit, students are able to:

  • Study DNA restriction enzyme function and use in genetic engineering
  • Use electrophoresis to separate DNA fragments
  • Use standard curves constructed from their data to make precise determinations of DNA fragment sizes
  • Read plasmid maps and predict the sizes of DNA fragments from restriction enzyme digests prior to performing the laboratory activity
  • Use restriction digest maps of lambda bacteriophage genomes (provided in the kit) to design novel plasmids
  • Discuss scientific, ethical, and legal implications of forensics, DNA profiling, and genetic engineering

How It Works

The six DNA samples in the Forensic DNA Fingerprinting Kit are plasmids engineered to mimic the natural variations in DNA that exist between one human being and another. One DNA sample has been collected from a "crime scene" and five samples have been obtained from various "suspects." Each sample is digested using a mixture of two DNA restriction enzymes, which generates a distinct set of DNA fragments for each sample. The resulting DNA fragments are separated by agarose gel electrophoresis and visualized using Bio-Rad's revolutionary Fast Blast™ DNA Stain.

Additional Information

  • HOSA: future health professionals

    This kit is a resource for the HOSA competitive event in Biotechnology. To learn more, visit HOSA.org or bio-rad.com/HOSA.

  • LT; Create, Share, Inspire.

    This hands-on activity is integrated with the Lt Biology Collection, the online learning platform from ADInstruments.

Specifications

# Workstations
8
# Students per workstation
4
# Lab sessions (45-minute labs)
2
Storage temperature (upon delivery)
Ambient or –20°C as indicated

Kit Contents

DNA Fingerprinting Kit Refill Package
DNA size standard (HindIII lambda DNA digest)
100 µl
Crime scene DNA sample, lyophilized
60 µg
Suspect 1 DNA sample, lyophilized
60 µg
Suspect 2 DNA sample, lyophilized
60 µg
Suspect 3 DNA sample, lyophilized
60 µg
Suspect 4 DNA sample, lyophilized
60 µg
Suspect 5 DNA sample, lyophilized
60 µg
EcoRl/Pstl restriction enzyme, lyophilized
3,000 U
Sample loading buffer
1 ml
Other Reagents and Consumables
TAE electrophoresis buffer (50x)
100 ml
Sterile water
2.5 ml
Agarose powder
5 g
Fast Blast DNA Stain, 500x
100 ml
Microcentrifuge tubes, 2.0 ml, multicolor
60
Microcentrifuge tubes, 1.5 ml
30
Foam floats
8
Instructions
Instructor and Student Guide
1
Fast Gel Protocol
1

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items

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1660007EDU
DNA evidence classroom study kit, includes DNA samples and standards, restriction enzymes, buffers, Fast Blast DNA stain, test tubes/holders, curriculum, and more, for 32 students; education use only

Refills

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1660027EDU
Consumable reagents for use with forensic DNA fingerprinting kit (1660007EDU), includes DNA samples/standard, restriction enzyme mix, sample buffer, sterile water; education use only
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166-0450EDU_tn.jpg
1660450EDU
Agarose gel preparation kit,makes 16–48 mini gels, includes agarose powder, Fast Blast DNA Stain, buffer; education use only
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166-0047EDU_tn.jpg
1660047EDU
3,000 units, lyophilized enzymes for use with forensic DNA fingerprinting kit (1660007EDU); education use only

Documents

Number Description
1660077EDU
Instruction Manual, Forensic DNA Fingerprinting Kit, Bio-Rad Explorer™
4106273
Forensic DNA Fingerprinting Kit, Lab Extension — Precut DNA Protocol, Bio-Rad Explorer™