Forensic DNA Fingerprinting Kit

Image
lsr_forensic_dna_fingerprints_kit.jpg

Overview

Using real DNA as evidence, your students play the role of crime scene investigator to figure out for themselves,
"Who done it?" This DNA kit for education illustrates the principles behind testing methods used by practicing for

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Description

Description

DNA gels

Run your DNA gels in 10 minutes!

Find Out How

DNA evidence assists in criminal, missing persons, mass disaster, and paternity cases. It can be used to identify a perpetrator or exonerate the innocent. The Forensic DNA Fingerprinting kit allows your students to use real DNA as evidence, as they play the role of crime scene investigator to figure out for themselves "Who done it?"

Features and Benefits

  • Aligns with AP Biology Big Idea 3, Lab 9
  • Two–session laboratory activity, 45 min per session
  • Provides sufficient materials for eight student workstations, up to four students per workstation

With this kit, students are able to:

  • Study DNA restriction enzyme function and use in genetic engineering
  • Use electrophoresis to separate DNA fragments
  • Use standard curves constructed from their data to make precise determinations of DNA fragment sizes
  • Read plasmid maps and predict the sizes of DNA fragments from restriction enzyme digests prior to performing the laboratory activity
  • Use restriction digest maps of lambda bacteriophage genomes (provided in the kit) to design novel plasmids
  • Discuss scientific, ethical, and legal implications of forensics, DNA profiling, and genetic engineering

How It Works

The six DNA samples in the Forensic DNA Fingerprinting Kit are plasmids engineered to mimic the natural variations in DNA that exist between one human being and another. One DNA sample has been collected from a "crime scene" and five samples have been obtained from various "suspects." Each sample is digested using a mixture of two DNA restriction enzymes, which generates a distinct set of DNA fragments for each sample. The resulting DNA fragments are separated by agarose gel electrophoresis and visualized using Bio-Rad's revolutionary Fast Blast™ DNA Stain.

Specifications

Specifications

Kit contains sufficient materials for 8 student workstations (2–4 students per workstation)
 
DNA size standard (HindIII lambda DNA digest), 100 µl
1
Crime scene DNA, 60 µg
1
Suspect DNA, 60 µg
5
EcoRl/Pstl restriction enzyme, lyophilized, 3,000 units
1
Sample loading buffer, 1 ml
1
Electrophoresis buffer, 50x TAE, 100 ml
1
Sterile water, 2.5 ml
1
Agarose powder, 5 g
1
Fast Blast™ DNA stain, 500x, 100 ml
1
Colored microcentrifuge tubes, 2.0 ml
60
Microcentrifuge tubes, 1.5 ml
30
Foam floats
8
Curriculum, including teacher's guide, student manual, and graphic quick guide
1

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items

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1660007EDU
DNA evidence classroom study kit, includes DNA samples and standards, restriction enzymes, buffers, Fast Blast DNA stain, test tubes/holders, curriculum, and more, for 32 students; education use only

Refills

Refills

166-0027EDU_tn.jpg
1660027EDU
Consumable reagents for use with forensic DNA fingerprinting kit (1660007EDU), includes DNA samples/standard, restriction enzyme mix, sample buffer, sterile water; education use only
166-0450EDU_tn.jpg
1660450EDU
Agarose gel preparation kit,makes 16–48 mini gels, includes agarose powder, Fast Blast DNA Stain, buffer; education use only
166-0047EDU_tn.jpg
1660047EDU
3,000 units, lyophilized enzymes for use with forensic DNA fingerprinting kit (1660007EDU); education use only

Documents

Documents

Number Description Options
1660077EDU
Instruction Manual, Forensic DNA Fingerprinting Kit, Bio-Rad Explorer™
3050
Forensic DNA Fingerprinting Kit, Quick Guide
6672
Map of AP-Aligned Bio-Rad Kits with Essential Knowledge
6673
Map of AP-Aligned Bio-Rad Kits with Investigative Laboratories
6605
Map of AP-Aligned Bio-Rad Kits with Learning Objectives
6580
20 Questions to Master Inquiry - Forensic DNA Fingerprinting Kit
6602
UView™ 6x Loading Dye and Stain Guide, Bio-Rad Explorer™
4106273
Forensic DNA Fingerprinting Kit, Lab Extension — Precut DNA Protocol, Bio-Rad Explorer™
5891
Refresh Kit Components — Large Class/Multiple Class Preparation Guide
5914
DNA Staining Using Fast Blast™ DNA Stain or SYBR® Safe Stain and Digital Analysis of Gel Images Using Logger Pro Software
5396
Fast Gel Protocol — Easily Run Your DNA Gels in Under 10 Minutes — Bio-Rad Explorer, Ver D
6916
Fast Protein and Nucleic Acid Electrophoresis Flier