Comparative Proteomics Kit I: Protein Profiler Module

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Overview

This module allows students to employ protein electrophoresis, the most widely used technique in life science research, to study protein structure and function.
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Description

Description

The Comparative Proteomics I Kit: Protein Profiler Module guides students through the thought processes involved in a laboratory-based scientific investigation. Students make predictions about their results in pre-lab activities using Internet databases and published phylogenetic information. They then employ protein electrophoresis, the most widely used technique in life science research, to study protein structure and function, generating protein profiles from the muscles of both distantly and closely related species of fish. From their results, they compare the different species' profiles, construct cladograms (phylogenetic trees), and assign each organism a branch. Students can decide whether their results support their predictions.

Features and Benefits

With this kit, students are able to:

  • Explore evolution
  • Study protein structure and function
  • Use polyacrylamide gel electrophoresis (PAGE) to separate proteins by size
  • Construct cladograms using data from their gel analysis

More Information

  • Aligns with AP Biology Big Idea 1
  • Three-session laboratory activity, 45 min per session
  • Provides sufficient materials for eight student workstations, up to four students per workstation

Specifications

Specifications

Kit contains sufficient materials for 8 student workstations (2–4 students per workstation)
 
Laemmli sample buffer
30 ml
Kaleidoscope™ Prestained Protein Standards
50 µl
10x Tris-Glycine-SDS electrophoresis buffer
1 L
Bio-Safe™ Coomassie Stain for proteins
2 x 100 ml
Actin and myosin standard, lyophilized
500 µg
Dithiothreitol (DTT)
0.3 g
Prot/Elect™ Pipet Tips for gel loading, 1–200 µl
1 rack of 96
1.5 ml fliptop micro test tube
60
1.5 ml screwcap micro test tube
50
Disposable plastic transfer pipets
30
Curriculum, including teacher's guide, student manual, and graphic quick guide
1

Ordering

Ordering

items

Use the filters below to refine results!

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1662700EDU
Protein profiling classroom study kit, includes buffers, standards, stains, DTT, test tubes/holders, disposable pipets/tips, trays, curriculum, and more, for 32 students; education use only
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1662850EDU
Comprehensive protein classroom study kit, includes Protein Profiler Module (1662700EDU) and Western Blot Module (1662800EDU), for 32 students; education use only
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1662875EDU
Comprehensive protein classroom study kit, includes Protein Profiler Module (1662700EDU), Western Blot Module (1662800EDU), Trans-Blot® Turbo Mini Nitrocellulose Transfer Packs (10), 4–20% Mini-PROTEAN® TGX Stain-Free Gels (10), application note, for 32 students; education use only
17006135EDU
Comparative Proteomics Kit and Stain-Free TGX Gels Combo, includes Comparative Proteomics Kit I: Protein Profiler Module (1662700EDU) and a pack of 10, 4-20% MP TGX Stain-Free Precast Gels (4568093EDU)
17006136EDU
Comparative Proteomics Kit and TGX Gels Combo, includes Comparative Proteomics Kit I: Protein Profiler Module (1662700EDU) and a pack of 10, 4-20% TGX Precast Gels (4561093EDU)

Refills

Refills

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4561093EDU
Pkg of 10, 4–20% precast polyacrylamide gel, 10-well, 30 µl, for use with Mini-PROTEAN electrophoresis cells; education use only
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1662701EDU
Consumables refill kit for use with Comparative Proteomics Kit I: Protein Profiler Module (1662700EDU), includes standards, actin and myosin standard, DTT; education use only
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1610375EDU
500 µl, mixture of 10 multicolor recombinant proteins (10–250 kD), including three bands (25, 50, 75 kD) that match the reference bands of other standards, 50 applications; education use only
1660010EDU
Refill kit for use with Comparative Proteomics Kit I: Protein Profiler Module (1662700EDU), includes only the actin and myosin standard; education use only
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1662876EDU
Application note for western blotting, describes rapid blotting using the Trans-Blot® Turbo Transfer System and V3 Western Workflow (stain-free blotting) methods; education use only Use Add to Cart to order a printed copy or download a free PDF.
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456-8093_tn.jpg
4568093EDU
Pkg of 10, 4–20% precast polyacrylamide gel, 10-well, 30 µl, for use with Mini-PROTEAN electrophoresis cells and stain-free enabled imagers; education use only

Documents

Documents

Number Description Options
6672
Map of AP-Aligned Bio-Rad Kits with Essential Knowledge
6673
Map of AP-Aligned Bio-Rad Kits with Investigative Laboratories
6605
Map of AP-Aligned Bio-Rad Kits with Learning Objectives
6582
20 Questions to Master Inquiry - Protein Profiler Kit, Rev A
M1662876EDU
Bio-Rad Explorer™ Rapid Blotting + V3 Western Workflow (Stain-Free Rapid Blotting) Application Note, Rev A
5464
Instruction Manual, Comparative Proteomics Kit I: Protein Profiler Module Quick Guide, Bio-Rad Explorer™, Rev A
10004530
Instruction Manual, Comparative Proteomics Kit I: Protein Profiler Module, Bio-Rad Explorer Rev F
6916
Fast Protein and Nucleic Acid Electrophoresis Flier
6933
Fast Electrophoresis and Western Blotting Flier