Comparative Proteomics Kit I: Protein Profiler Module

Overview

Kit contains sufficient materials for 8 student workstations. (2–4 students per workstation)
Laemmli sample buffer	30 ml
Precision Plus Protein™ Kaleidoscope™ Prestained Standards	50 µl
10x Tris-glycine-SDS electrophoresis buffer	1 L
Bio-Safe™ Coomassie Stain for proteins	2 x 100 ml
Actin and myosin standard, lyophilized	500 µg
Dithiothreitol (DTT)	0.3 g
Prot/Elect™ Pipet Tips for gel loading	1 rack
1.5 ml fliptop micro test tube	60
1.5 ml screwcap micro test tube	50
Disposable plastic transfer pipets	30
Curriculum including Teacher's Guide, Student Manual, and graphic Quick Guide	1
Required Accessories Not Included in Kit:
Fish samples	5–8 types
Adjustable micropipets, 2–20 µl	8
Power supplies	2–4
Water bath	1
Gel-staining trays	4
Foam micro test tube racks	8
If using polyacrylamide gel electrophoresis: Required Accessories:
Vertical gel electrophoresis chambers	2–4
Mini-PROTEAN^® TGX™ Precast Polyacrylamide Gels, 4–20%, 10-well	8
If using agarose gel electrophoresis: Required Accessories
Horizontal gel electrophoresis chambers	4–8
Low-melt agarose	25 g
Pipet tips, 2–20 µl, TBR-35
Acetic acid	100 ml
Reagent alcohol/ethanol	400 ml
Recommended (Optional) Accessories:
Sample loading guides
GelAir™ Drying System

Protein Profiler Flowchart

Key Kit Features

Explore evolution
Study protein structure/function
Apply protein electrophoresis
Construct cladograms
Complete in three 45 minute lab sessions

Curriculum Fit

Protein Profiler Curriculum Fit

Theory of Evolution

This kit guides students through the thought processes involved in a laboratory-based scientific investigation. Students are asked: Can molecular evidence support the theory of evolution? Why or why not? What explanations can you suggest?

Changes in proteins can reflect changes in the gene pool. Actin and myosin are the major muscle proteins essential for locomotion and survival in all animals. Muscle consists mainly of actin and myosin, but numerous other proteins also comprise muscle tissue. The structures and functions of actin and myosin have remained relatively stable or "conserved" in all animals over evolutionary time. However, other muscle proteins exhibit considerable variation even among closely related species. Detectable variations between organisms' protein profiles reflect physiological adaptations to different environments, but they originate as random DNA mutations. Such mutation events, if favorable, persist through the natural selection process and contribute to the evolution of species — with new specialized functions.

Mutation > Variation > Specialization > Speciation = Evolution

This is an open-ended inquiry-based kit. Students make predictions about their results in prelab activities using internet databases and published phylogenetic information. They generate novel results and apply their findings directly to the problem of solving evolutionary relationships by constructing cladograms (phylogenetic trees). From their gel data, they build up a tree and assign each organism a branch. Students can decide whether their results support their predictions.

Protein Profiler Gel

PowerPoint Presentation for Educational Use

PowerPoint Presentation for Workshops and for Teaching

Comparative Proteomics Kit I: Protein Profiler Module

This PowerPoint presentation is intended for educational use only. The presentation provides educators with background on the Comparative Proteomics Kit I: Protein Profiler Module and includes instructions for using the kit.

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The Comparative Proteomics I Kit: Protein Profiler Module guides students through the thought processes involved in a laboratory-based scientific investigation. Students make predictions about their results in pre-lab activities using Internet databases and published phylogenetic information. They then employ protein electrophoresis, the most widely used technique in life science research, to study protein structure and function, generating protein profiles from the muscles of both distantly and closely related species of fish. From their results, they compare the different species' profiles, construct cladograms (phylogenetic trees), and assign each organism a branch. Students can decide whether their results support their predictions.

Features and Benefits

With this kit, students are able to:

Explore evolution
Study protein structure and function
Use polyacrylamide gel electrophoresis (PAGE) to separate proteins by size
Construct cladograms using data from their gel analysis

More Information

Aligns with AP Biology Big Idea 1
Three-session laboratory activity, 45 min per session
Provides sufficient materials for eight student workstations, up to four students per workstation

Kit contains sufficient materials for 8 student workstations (2–4 students per workstation)
Laemmli sample buffer	30 ml
Kaleidoscope™ Prestained Protein Standards	50 µl
10x Tris-Glycine-SDS electrophoresis buffer	1 L
Bio-Safe™ Coomassie Stain for proteins	2 x 100 ml
Actin and myosin standard, lyophilized	500 µg
Dithiothreitol (DTT)	0.3 g
Prot/Elect™ Pipet Tips for gel loading, 1–200 µl	1 rack of 96
1.5 ml fliptop micro test tube	60
1.5 ml screwcap micro test tube	50
Disposable plastic transfer pipets	30
Curriculum, including Teacher's Guide, Student Manual, and graphic Quick Guide	1

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Comparative Proteomics Kit I: Protein Profiler Module

1662700EDU
Protein profiling classroom study kit, includes buffers, standards, stains, DTT, test tubes/holders, disposable pipets/tips, trays, curriculum, and more, for 32 students; education use only

0 1662700EDU /templatedata/internet/product_SKU/data/LSE/Protein-Analysis-Kits/Comparative-Proteomics-Kit-I--/166-2700EDU_0921121041849.xml 1662700EDU /default/main/bio-rad/WORKAREA/default 09/21/09 12:10 PM en_US jthvlbja 2017-11-14 07:07:24 true true internet/product_SKU jthvlbja templatedata/internet/product_SKU/data/LSE/Protein-Analysis-Kits/Comparative-Proteomics-Kit-I--/166-2700EDU_0921121041849.xml LSE 2019-05-04 10:45:19 /default/main/bio-rad/STAGING N /default/main/bio-rad en 12/31/37 11:59 PM 1555972636264 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN 2019-05-04 10:45:19 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSE/Protein-Analysis-Kits/Comparative-Proteomics-Kit-I--/166-2700EDU_0921121041849.xml templatedata/internet/product_SKU/data/LSE/Protein-Analysis-Kits/Comparative-Proteomics-Kit-I--/166-2700EDU_0921121041849.xml 1662700edu-comparative-proteomics-kit-i-protein-profiler-module 1662700EDU Comparative Proteomics Kit I: Protein Profiler Module Comparative Proteomics Kit I: Protein Profiler Module Protein profiling classroom study kit, includes buffers, standards, stains, DTT, test tubes/holders, disposable pipets/tips, trays, curriculum, and more, for 32 students; education use only Protein profiling classroom study kit, includes buffers, standards, stains, DTT, test tubes/holders, disposable pipets/tips, trays, curriculum, and more, for 32 students; education use only Protein profiling classroom study kit, includes buffers, standards, stains, DTT, test tubes/holders, disposable pipets/tips, trays, curriculum, and more, for 32 students; education use only /webroot/web/images/lse/products/protein_analysis_kits/sku_thumb/global/166-2700EDU_tn.jpg /webroot/web/images/lse/products/protein_analysis_kits/sku_view/global/166-2700EDU_view.jpg /webroot/web/images/lse/products/protein_analysis_kits/sku_feature/global/166-2700EDU_feature.jpg RepEx 166-2700EDU Protein profiling classroom study kit, includes buffers, standards, stains, DTT, test tubes/holders, disposable pipets/tips, trays, curriculum, and more, for 32 students; education use only 09/21/09 12:10 PM 12/31/37 11:59 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSE /LSE/Protein-Analysis-Kits/Comparative-Proteomics-Kit-I-- N 0 1662700, 1662700EDU, comparative, proteomics, protein, profiler, module, analysis, structure, function, sds, page, polyacrylamide, gel, electrophoresis, separation, science, education, products, classroom /en-us/education/promotions/free-starter-kit-with-purchase-protein-electrophoresis-lab-set false $222.50 true $ %24178.00 true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1662700EDU&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1662700EDU&CartQuote=QT&quantity= 1711 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1662700EDU&AddToDefaultWishList=true&quantity= 12161500 222.50 1662700EDU

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Comparative Proteomics Kits I and II: Protein Profiler Module and Western Blot Module

1662850EDU
Comprehensive protein classroom study kit, includes Protein Profiler Module (1662700EDU) and Western Blot Module (1662800EDU), for 32 students; education use only

1 1662850EDU /templatedata/internet/product_SKU/data/LSE/Protein-Analysis-Kits/Comparative-Proteomics-Kit-I--/166-2850EDU_0921121041828.xml 1662850EDU /default/main/bio-rad/WORKAREA/default 09/21/09 12:10 PM en_US jthvlbkg 2017-11-14 07:07:24 true true internet/product_SKU jthvlbkg templatedata/internet/product_SKU/data/LSE/Protein-Analysis-Kits/Comparative-Proteomics-Kit-I--/166-2850EDU_0921121041828.xml LSE 2019-05-04 10:45:30 /default/main/bio-rad/STAGING N /default/main/bio-rad en 12/31/37 11:59 PM 1555972636348 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN 2019-05-04 10:45:30 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSE/Protein-Analysis-Kits/Comparative-Proteomics-Kit-I--/166-2850EDU_0921121041828.xml templatedata/internet/product_SKU/data/LSE/Protein-Analysis-Kits/Comparative-Proteomics-Kit-I--/166-2850EDU_0921121041828.xml 1662850edu-comparative-proteomics-kits-i-ii-protein-profiler-module-western-blot-module 1662850EDU Comparative Proteomics Kits I and II: Protein Profiler Module and Western Blot Module Comparative Proteomics Kits I and II: Protein Profiler Module and Western Blot Module Comprehensive protein classroom study kit, includes Protein Profiler Module (1662700EDU) and Western Blot Module (1662800EDU), for 32 students; education use only Comprehensive protein classroom study kit, includes Protein Profiler module (#166-2700EDU) and Western Blot module (#166-2800EDU), for 32 students; education use only Comprehensive protein classroom study kit, includes Protein Profiler Module (1662700EDU) and Western Blot Module (1662800EDU), for 32 students; education use only /webroot/web/images/lse/products/protein_analysis_kits/sku_thumb/global/166-2850EDU_tn.jpg /webroot/web/images/lse/products/protein_analysis_kits/sku_view/global/166-2850EDU_view.jpg /webroot/web/images/lse/products/protein_analysis_kits/sku_feature/global/166-2850EDU_feature.jpg ,VN RepEx 166-2850EDU Comprehensive protein classroom study kit, includes Protein Profiler module (#166-2700EDU) and Western Blot module (#166-2800EDU), for 32 students; education use only 1662850, 1662850EDU, comparative, proteomics, and, ii, protein, profiler, module, western, blot, analysis, structure, function, sds, page, separation, westerns, blots, blotting, immunodetection, science, education, products, classroom 09/21/09 12:10 PM 12/31/37 11:59 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSE /LSE/Protein-Analysis-Kits/Comparative-Proteomics-Kit-I-- N 0 false $462.50 true $ %24370.00 true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1662850EDU&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1662850EDU&CartQuote=QT&quantity= 1711 true true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1662850EDU&AddToDefaultWishList=true&quantity= 41105300 462.50 1662850EDU

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Rapid Blotting and V3 Western Workflow™ Starter Kit

1662875EDU
Comprehensive protein classroom study kit, includes Protein Profiler Module (1662700EDU), Western Blot Module (1662800EDU), Trans-Blot^® Turbo™ Mini Nitrocellulose Transfer Packs (10), 4–20% Mini-PROTEAN^® TGX Stain-Free™ Gels (10), application note, for 32 students; education use only

1662875EDU /templatedata/internet/product_SKU/data/LSE/Equipment-and-Supplies/Imaging/166-2875EDU_1344282022.xml 1662875EDU /default/main/bio-rad/WORKAREA/default 08/06/12 09:55 AM en_US juso9kg3 2017-11-14 07:07:19 true true internet/product_SKU juso9kg3 templatedata/internet/product_SKU/data/LSE/Equipment-and-Supplies/Imaging/166-2875EDU_1344282022.xml LSE 2019-05-04 11:12:59 /default/main/bio-rad/STAGING N /default/main/bio-rad en 03/06/22 12:39 AM 1555972099887 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN 2019-05-04 11:12:59 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSE/Equipment-and-Supplies/Imaging/166-2875EDU_1344282022.xml templatedata/internet/product_SKU/data/LSE/Equipment-and-Supplies/Imaging/166-2875EDU_1344282022.xml 1662875edu-rapid-blotting-v3-western-workflow-starter-kit 1662875EDU Rapid Blotting and V3 Western Workflow Starter Kit Rapid Blotting and V3 Western Workflow Starter Kit Comprehensive protein classroom study kit, includes Protein Profiler Module (1662700EDU), Western Blot Module (1662800EDU), Trans-Blot<sup>®</sup> Turbo Mini Nitrocellulose Transfer Packs (10), 4–20% Mini-PROTEAN<sup>®</sup> TGX Stain-Free Gels (10), application note, for 32 students; education use only Comprehensive protein classroom study kit, includes Protein Profiler Module (1662700EDU), Western Blot Module (1662800EDU), Trans-Blot<sup>®</sup> Turbo Mini Nitrocellulose Transfer Packs (10), 4–20% Mini-PROTEAN<sup>®</sup> TGX Stain-Free Gels (10), application note, for 32 students; education use only /webroot/web/images/lse/products/protein_analysis_kits/sku_thumb/global/166-2875EDU_tn.jpg /webroot/web/images/lse/products/protein_analysis_kits/sku_view/global/166-2875EDU_view.jpg /webroot/web/images/lse/products/protein_analysis_kits/sku_feature/global/166-2875EDU_feature.jpg ,VN Molly Gregg 166-2875EDU Comprehensive protein classroom study kit, includes Protein Profiler Module (#166-2700EDU), Western Blot Module (#166-2800EDU), Trans-Blot<sup>®</sup> Turbo mini nitrocellulose transfer packs (10), 4–20% Mini-PROTEAN<sup>®</sup> TGX Stain-Free gels (10), application note, for 32 students; education use only Comprehensive protein classroom study kit, includes protein profiler module (#166-2700EDU), western blot module (#166-2800EDU), Trans-Blot<sup>®</sup> Turbo™ mini nitrocellulose transfer pack, TGX Stain-Free gels, application note, for 32 students; education use only 08/06/12 09:55 AM 03/06/22 12:39 AM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSE /LSE/Equipment-and-Supplies/Imaging N 0 <p><strong>Western blotting in less than 2 hours!</strong> Using our new rapid blotting or V3 Western Workflow (stain-free rapid blotting) option allows you to complete the entire western blot workflow in less than 2–5 hours, depending on which time-saving steps you incorporate. Mini-PROTEAN TGX Stain-Free gels (4–20%) combined with our superfast Trans-Blot Turbo transfer system provide the fastest speed and most time savings, allowing you to complete the workflow in less than a single 3-hr lab block. Teach your students about the exciting new chemistry that allows visualization of samples separated on PAGE gels without staining! To learn more, download the <a href="/webroot/web/pdf/lse/literature/M1662876EDU.pdf" target="_blank"> Rapid Blotting + V3 Western Workflow<sup>™</sup> Application Note.</a></p> /webroot/web/html/lse/products/SKUs/autopopulated 166-2875EDU_1344282022_XX_en.xml /webroot/web/html/lse/products/SKUs/autopopulated/166-2875EDU_1344282022_XX_en.xml Kit contents 2807 Sufficient materials for 8 student workstations with 2–4 students per workstation, plus additional reagents for further studies 1 0 Kit I 2812 Comparative Proteomics Kit I: Protein Profiler Module, 1 1 0 Kit II 2813 Comparative Proteomics Kit II: Western Blot Module, 1 1 0 Transfer packs 2815 Trans-Blot Turbo mini nitrocellulose transfer packs, 1 pkg of 10 1 0 Gels 2811 4–20% Mini-PROTEAN TGX Stain-Free gels, 1 pkg of 10 1 0 Application note 2810 Rapid Blotting + V3 Western Workflow<sup>™</sup> Application Note, 1 1 0 Notes 2814 Obtain fish samples locally. Mini-PROTEAN TGX Stain-Free gels require a UV imager to visualize the resolved protein samples. Traditional SDS-PAGE gels can be used in place of Mini-PROTEAN TGX Stain-Free gels but require staining/destaining of gels to visualize the resolved protein samples. 1 0 false $717.50 true $ %24574.00 true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1662875EDU&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1662875EDU&CartQuote=QT&quantity= 1711 true true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1662875EDU&AddToDefaultWishList=true&quantity= 41105300 717.50 1662875EDU

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4–20% Mini-PROTEAN^® TGX™ Precast Gel

4561093EDU
Pkg of 10, 4–20% precast polyacrylamide gel, 10-well, 30 µl, for use with Mini-PROTEAN Electrophoresis Cells; education use only

4561093EDU /templatedata/internet/product_SKU/data/LSE/Equipment-and-Supplies/Reagents/456-1093EDU_1292456516.xml 4561093EDU /default/main/bio-rad/WORKAREA/default 02/10/11 05:05 PM en_US jdl0t1hw 2018-02-01 00:09:36 true true internet/product_SKU jdl0t1hw templatedata/internet/product_SKU/data/LSE/Equipment-and-Supplies/Reagents/456-1093EDU_1297386482.xml LSE 2019-05-04 10:30:01 /default/main/bio-rad/STAGING N /default/main/bio-rad en 02/10/21 05:07 PM 1555970223070 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN 2019-05-04 10:30:01 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSE/Equipment-and-Supplies/Reagents/456-1093EDU_1297386482.xml templatedata/internet/product_SKU/data/LSE/Equipment-and-Supplies/Reagents/456-1093EDU_1297386482.xml 4561093edu-4-20-mini-protean-tgx-precast-gel 4561093EDU Mini-PROTEAN TGX Precast Gels 4–20% Mini-PROTEAN<sup>®</sup> TGX™ Precast Gel Pkg of 10, 4–20% precast polyacrylamide gel, 10-well, 30 µl, for use with Mini-PROTEAN Electrophoresis Cells; education use only /webroot/web/images/lsr/products/electrophoresis/sku_thumb/global/456-1093_tn.jpg /webroot/web/images/lsr/products/electrophoresis/sku_view/global/456-1093_view.jpg /webroot/web/images/lsr/products/electrophoresis/sku_feature/global/456-1093_feature.jpg 4–20% Mini-PROTEAN® TGX™ Precast Gel ,VN Tommy Alvaran 4–20% Mini-PROTEAN® TGX™ Precast Gel 456-1093EDU, 4561093EDU, 4-20%, mini-protean, tgx, precast, protein, gels, 10-well, 30, tris, glycine, gel, mini, protean, mini-gel, polyacrylamide, page, sds, sds-page, electrophoresis, science, education, products, classroom 02/10/11 05:05 PM 02/10/21 05:07 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSE /LSE/Equipment-and-Supplies/Reagents N 0 <div class="fragment description ui-tabs-panel"> <p>This package contains ten 4–20% Mini-PROTEAN<sup>®</sup> TGX™ Precast Polyacrylamide Gels with a 10-well comb. <a href="/en-us/sku/4560000-mini-protean-cassette-opening-lever?ID=4560000">Gel opening levers (4560000)</a> for opening Mini-PROTEAN Precast Gel Cassettes are sold separately.</p> <p>The 12-month shelf life Mini-PROTEAN TGX Precast Gels are an innovative PAGE/SDS-PAGE System. They are designed to provide Laemmli-like separation patterns using a standard Tris-glycine Running Buffer for protein gel electrophoresis. These gels are for use with Mini-PROTEAN Electrophoresis Cells.</p> </div> /webroot/web/html/lse/products/SKUs/autopopulated 456-1093edu_description.html /webroot/web/html/lse/products/SKUs/autopopulated/456-1093edu_description.html Comb 1462 10-well 1 0 Shelf life, months* 2710 12 1 0 Gel storage conditions 1693 Store flat at 2–8°C; do not freeze 1 0 Recommended sample buffer (Laemmli, dilute 1:1 with sample) 2625 62.5 mM Tris-HCl, pH 6.8, 2% SDS, 25% glycerol, 0.01% bromophenol blue 1 0 Recommended running buffer (Tris/glycine/SDS) 2626 25 mM Tris, 192 mM glycine, 0.1% SDS, pH 8.3 1 0 Gel dimensions (W x L x thickness) (cm) 1684 8.6 x 6.7 x 0.1 1 0 Cassette dimensions (W x L x thickness) (cm) 1413 10 x 8 x 0.46 1 0 Cassette material 1415 Styrene copolymer 1 0 Comb material 1464 Polycarbonate 1 0 * From date of manufacture. 1096   1 0 false $116.00 true $ %2492.80 true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=4561093EDU&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=4561093EDU&CartQuote=QT&quantity= 1717 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=4561093EDU&AddToDefaultWishList=true&quantity= 41105300 116.00 4561093EDU

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Protein Profiler Temperature Sensitive Reagents Bag

1662701EDU
Consumables refill kit for use with Comparative Proteomics Kit I: Protein Profiler Module (1662700EDU), includes standards, actin and myosin standard, DTT; education use only

1662701EDU /templatedata/internet/product_SKU/data/LSE/Protein-Analysis-Kits/Comparative-Proteomics-Kit-I--/166-2701EDU_0921121041870.xml 5 1662701EDU /default/main/bio-rad/WORKAREA/default 09/21/09 12:10 PM en_US jthvlb70 2017-11-14 07:07:24 true true internet/product_SKU jthvlb70 templatedata/internet/product_SKU/data/LSE/Protein-Analysis-Kits/Comparative-Proteomics-Kit-I--/166-2701EDU_0921121041870.xml LSE 2019-05-04 10:44:13 /default/main/bio-rad/STAGING N /default/main/bio-rad en 12/31/37 11:59 PM 1555972636306 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN 2019-05-04 10:44:13 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSE/Protein-Analysis-Kits/Comparative-Proteomics-Kit-I--/166-2701EDU_0921121041870.xml templatedata/internet/product_SKU/data/LSE/Protein-Analysis-Kits/Comparative-Proteomics-Kit-I--/166-2701EDU_0921121041870.xml 1662701edu-protein-profiler-temperature-sensitive-reagents-bag 1662701EDU Protein Profiler Temperature Sensitive Reagents Bag Protein Profiler Temperature Sensitive Reagents Bag Consumables refill kit for use with Comparative Proteomics Kit I: Protein Profiler Module (1662700EDU), includes standards, actin and myosin standard, DTT; education use only Consumables refill kit for use with Comparative Proteomics Kit I: Protein Profiler Module (#166-2700EDU), contains standards, actin and myosin standard, and DTT; education use only Consumables refill kit for use with Comparative Proteomics Kit I: Protein Profiler Module (1662700EDU), includes standards, actin and myosin standard, DTT; education use only /webroot/web/images/lse/products/protein_analysis_kits/sku_thumb/global/166-2701EDU_tn.jpg /webroot/web/images/lse/products/protein_analysis_kits/sku_view/global/166-2701EDU_view.jpg /webroot/web/images/lse/products/protein_analysis_kits/sku_feature/global/166-2701EDU_feature.jpg ,VN RepEx 166-2701EDU Consumables refill kit for use with Comparative Proteomics Kit I: Protein Profiler Module (#166-2700EDU), contains standards, actin and myosin standard, and DTT; education use only 1662701, 1662701EDU, protein, profiler, temperature, sensitive, reagents, bag, refill, comparative, proteomics, module, standards, actin, myosin, science, education, products, classroom 09/21/09 12:10 PM 12/31/37 11:59 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSE /LSE/Protein-Analysis-Kits/Comparative-Proteomics-Kit-I-- N 0 <p>Protein Profiler Temperature-Sensitive Reagents Bag is a consumables refill kit for use with Comparative Proteomics Kit I: Protein Profiler Module (#1662700EDU). The bag contains prestained standards, actin and myosin standard, and DTT. This product is for education use only.</p> /webroot/web/html/lse/products/SKUs/autopopulated 166-2701EDU_0921121041870_en.html /webroot/web/html/lse/products/SKUs/autopopulated/166-2701EDU_0921121041870_en.html Precision Plus Protein™ Kaleidoscope™ prestained standards, 50 µl 2488 1 1 0 Actin and myosin standard, 500 µg lyophilized 1324 1 1 0 Dithiothreitol (DTT), 0.3 g 2489 1 1 0 false $96.25 true $ %2477.00 true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1662701EDU&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1662701EDU&CartQuote=QT&quantity= 1711 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1662701EDU&AddToDefaultWishList=true&quantity= 12161500 96.25 1662701EDU

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2x Laemmli Sample Buffer

1610737EDU
30 ml, premixed protein sample buffer for SDS-PAGE; education use only

1610737EDU /templatedata/internet/product_SKU/data/LSE/Equipment-and-Supplies/Reagents/161-0737EDU_0921121020190.xml 4 1610737EDU /default/main/bio-rad/WORKAREA/default 09/21/09 12:10 PM en_US juso9kd9 2018-02-01 00:09:36 true true internet/product_SKU juso9kd9 templatedata/internet/product_SKU/data/LSE/Equipment-and-Supplies/Reagents/161-0737EDU_0921121020190.xml LSE 2019-05-04 10:29:54 /default/main/bio-rad/STAGING N /default/main/bio-rad en 12/31/37 11:59 PM 1555970215374 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN 2019-05-04 10:29:54 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSE/Equipment-and-Supplies/Reagents/161-0737EDU_0921121020190.xml templatedata/internet/product_SKU/data/LSE/Equipment-and-Supplies/Reagents/161-0737EDU_0921121020190.xml 1610737edu-2x-laemmli-sample-buffer 1610737EDU Laemmli Sample Buffer 2x Laemmli Sample Buffer 30 ml, premixed protein sample buffer for SDS-PAGE; education use only 30 ml, premixed protein sample buffer for SDS-PAGE; education use only 30 ml, premixed protein sample buffer for SDS-PAGE; education use only /webroot/web/images/lse/products/equipment_supplies/sku_thumb/global/161-0737EDU_tn.jpg /webroot/web/images/lse/products/equipment_supplies/sku_view/global/161-0737EDU_view.jpg /webroot/web/images/lse/products/equipment_supplies/sku_feature/global/161-0737EDU_feature.jpg ,VN RepEx 161-0737EDU 30 ml, premixed protein sample buffer for SDS-PAGE; education use only 161-0737EDU, 1610737EDU, 2x, laemmli, sample, buffer, premixed, sds, sds-page, page, polyacrylamide, gel, electrophoresis, science, education, products 09/21/09 12:10 PM 12/31/37 11:59 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSE /LSE/Equipment-and-Supplies/Reagents N 0 <p>Dilute protein samples 1:1 in 2x Laemmli sample buffer before loading on SDS-PAGE gels (requires the addition of β-mercaptoethanol).</p> <p><strong>Other Information:</strong><br /></p> <ul> <li>Formulation: 65.8 mM Tris-HCl, pH 6.8, 2.1% SDS, 26.3% (w/v) glycerol, 0.01% bromophenol blue</li> <li>Ready to dilute — use distilled deionized water</li> <li>Premixed loading buffer — ensures lane-to-lane consistency</li> <li>Quality-controlled reagent — guarantees reproducible results</li> </ul> <p><strong>Related Products</strong></p> <p>We offer additional <a href="/evportal/destination/commerce/product_detail?catID=09e4dbf1-40c5-4a92-ace5-3ec31296ac7e">sample buffers and reagents</a> for protein electrophoresis including the following sample buffers:</p> <ul> <li><a href="/en-us/sku/1610747-4x-laemmli-sample-buffer?ID=1610747">4x Laemmli sample buffer (1610747)</a></li> <li><a href="/en-us/sku/1610738edu-native-page-sample-buffer?ID=1610738EDU">Native PAGE sample buffer (1610738EDU)</a></li> <li><a href="/en-us/sku/1610739-tricine-sample-buffer-for-protein-gels-30-ml?ID=1610739">Tricine sample buffer (1610739)</a></li> <li><a href="/en-us/sku/1610763-ief-sample-buffer-for-vertical-gels-30-ml?ID=1610763">IEF sample buffer (1610763)</a></li> <li><a href="/en-us/sku/1610764-zymogram-sample-buffer-30-ml?ID=1610764">Zymogram sample buffer (1610764)</a></li> <li><a href="/en-us/sku/1610791-xt-sample-buffer?ID=1610791">XT sample buffer (1610791)</a></li> </ul> <h2 class="descHeader">Supporting Documentation</h2> <p><a href="/webroot/web/pdf/lsr/literature/4006028.pdf" target="_blank">Download the Laemmli sample buffer instruction manual</a>.</p> <h2 class="descHeader">Instructions for Use:</h2> <p><strong>Step 1:</strong> Prepare 2x sample buffer containing β-mercaptoethanol (BME) by mixing 950 µl of 2x Laemmli sample buffer (161-0737) with 50 µl of BME (1610710);</p> <p><strong>Step 2:</strong> Dilute protein sample 1:1 in sample buffer from Step 1. For example: 100 µl protein sample + 100 µl 2x sample buffer containing BME;</p> <p><strong>Step 3:</strong> Heat sample at 95°C for 5 min. Load samples onto SDS-PAGE gels.</p> <ul> <br /> </ul> /webroot/web/html/lse/products/SKUs/autopopulated 161-0737EDU_0921121020190_en.html /webroot/web/html/lse/products/SKUs/autopopulated/161-0737EDU_0921121020190_en.html Laemmli sample buffer, 30 ml 2487 1 1 0 false $14.00 true $ %2411.20 true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1610737EDU&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1610737EDU&CartQuote=QT&quantity= 1717 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1610737EDU&AddToDefaultWishList=true&quantity= 12161700 14.00 1610737EDU

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Precision Plus Protein^™ Kaleidoscope^™ Standards

1610375EDU
500 µl, mixture of 10 multicolor recombinant proteins (10–250 kD), including three bands (25, 50, 75 kD) that match the reference bands of other standards, 50 applications; education use only

1610375EDU /templatedata/internet/product_SKU/data/LSE/Equipment-and-Supplies/Reagents/161-0375EDU_0921121023611.xml 3 1610375EDU /default/main/bio-rad/WORKAREA/default 09/21/09 12:10 PM en_US juso9kd1 2018-02-01 00:09:36 true true internet/product_SKU juso9kd1 templatedata/internet/product_SKU/data/LSE/Equipment-and-Supplies/Reagents/161-0375EDU_0921121023611.xml LSE 2019-05-04 10:29:10 /default/main/bio-rad/STAGING N /default/main/bio-rad en 12/31/37 11:59 PM 1555970214537 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN 2019-05-04 10:29:10 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSE/Equipment-and-Supplies/Reagents/161-0375EDU_0921121023611.xml templatedata/internet/product_SKU/data/LSE/Equipment-and-Supplies/Reagents/161-0375EDU_0921121023611.xml 1610375edu-precision-plus-protein-kaleidoscope-standards 1610375EDU Precision Plus Protein Kaleidoscope Standards Precision Plus Protein<sup>™</sup> Kaleidoscope<sup>™</sup> Standards 500 µl, mixture of 10 multicolor recombinant proteins (10–250 kD), including three bands (25, 50, 75 kD) that match the reference bands of other standards, 50 applications; education use only 500 µl, mixture of 10 multicolor recombinant proteins (10–250 kD), including three bands (25, 50, 75 kD) that match the reference bands of other standards, 50 applications; education use only 500 µl, mixture of 10 multicolor recombinant proteins (10–250 kD), including three bands (25, 50, 75 kD) that match the reference bands of other standards, 50 applications; education use only /webroot/web/images/lsr/products/electrophoresis/sku_thumb/global/161-0375_tn.jpg /webroot/web/images/lsr/products/electrophoresis/sku_view/global/161-0375_view.jpg /webroot/web/images/lsr/products/electrophoresis/sku_feature/global/161-0375_feature.jpg ,VN RepEx 161-0375EDU 500 µl, mixture of 10 multicolor recombinant proteins (10–250 kD), including three bands (25, 50, 75 kD) that match the reference bands of other standards, 50 applications; education use only 161-0375EDU, 1610375EDU, precision plus protein, kaleidoscope, standards, multicolor, standard, markers, marker, molecular, weight, mw, ladders, ladder, prestained, science, education, productspeptide, polypeptide 09/21/09 12:10 PM 12/31/37 11:59 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSE /LSE/Equipment-and-Supplies/Reagents N 0 <p>Precision Plus Protein™ Kaleidoscope™ Standards (500 µl, 50 applications) are a mixture of 10 multicolor recombinant proteins (10–250 kD) and include three bands (25, 50, 75 kD) that match the reference bands of other standards.</p> <p>Precision Plus Protein Kaleidoscope Standards can be used as a refill for the <a href="/en-us/sku/1660013edu-pglo-kit-sds-page-extension?ID=1660013EDU">pGLO™ Kit</a> SDS-PAGE Extension (<a href="/en-us/sku/1660013edu-pglo-kit-sds-page-extension?ID=1660013EDU">#1660013EDU</a>), <a href="/en-us/sku/1662700edu-comparative-proteomics-kit-i-protein-profiler-module?ID=1662700EDU">Comparative Proteomics Kit I:</a> Protein Profiler Module (<a href="/en-us/sku/1662700edu-comparative-proteomics-kit-i-protein-profiler-module?ID=1662700EDU">#1662700EDU</a>), and the <a href="/en-us/sku/1662850edu-comparative-proteomics-kits-i-ii-protein-profiler-module-western-blot-module?ID=1662850EDU">Comparative Proteomics Kits I and II:</a> Protein Profiler Module and Western Blot Module (<a href="/en-us/sku/1662850edu-comparative-proteomics-kits-i-ii-protein-profiler-module-western-blot-module?ID=1662850EDU">#1662850EDU</a>). This product is for education use only.</p> /webroot/web/html/lse/products/SKUs/autopopulated 161-0375EDU_0921121023611_en.html /webroot/web/html/lse/products/SKUs/autopopulated/161-0375EDU_0921121023611_en.html 4561083EDU /templatedata/internet/product_SKU/data/LSE/Equipment-and-Supplies/Electrophoresis-and-Blotting-A/456-1083EDU_1343345186.xml 1610732EDU /templatedata/internet/product_SKU/data/LSE/Equipment-and-Supplies/Reagents/161-0732EDU_0921121020153.xml 1610737EDU /templatedata/internet/product_SKU/data/LSE/Equipment-and-Supplies/Reagents/161-0737EDU_0921121020190.xml false $148.00 true $ %24111.00 true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1610375EDU&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1610375EDU&CartQuote=QT&quantity= 1717 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1610375EDU&AddToDefaultWishList=true&quantity= 12161500 148.00 1610375EDU

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Dithiothreitol

1610610EDU
1 g, dithiothreitol (DTT) reducing agent; education use only

1610610EDU /templatedata/internet/product_SKU/data/LSE/Equipment-and-Supplies/Reagents/161-0610EDU_0921121024180.xml 2 1610610EDU /default/main/bio-rad/WORKAREA/default 09/21/09 12:10 PM en_US jthvlb6m 2017-11-14 07:07:21 true true internet/product_SKU jthvlb6m templatedata/internet/product_SKU/data/LSE/Equipment-and-Supplies/Reagents/161-0610EDU_0921121024180.xml LSE 2019-05-04 10:44:08 /default/main/bio-rad/STAGING N /default/main/bio-rad en 12/31/37 11:59 PM 1555970214899 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN 2019-05-04 10:44:08 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSE/Equipment-and-Supplies/Reagents/161-0610EDU_0921121024180.xml templatedata/internet/product_SKU/data/LSE/Equipment-and-Supplies/Reagents/161-0610EDU_0921121024180.xml 1610610edu-dithiothreitol 1610610EDU Dithiothreitol Dithiothreitol 1 g, dithiothreitol (DTT) reducing agent; education use only 1 g, dithiothreitol (DTT) reducing agent; education use only 1 g, dithiothreitol (DTT) reducing agent; education use only /webroot/web/images/lse/products/equipment_supplies/sku_thumb/global/161-0610EDU_tn.jpg /webroot/web/images/lse/products/equipment_supplies/sku_view/global/161-0610EDU_view.jpg /webroot/web/images/lse/products/equipment_supplies/sku_feature/global/161-0610EDU_feature.jpg RepEx 161-0610EDU 1 g, dithiothreitol (DTT) reducing agent; education use only 1610610, 1610610EDU, dithiothreitol, dtt, reducing, agent, cleland's, reagent, redox, antioxidant, stabilizer, electrophoresis, buffer, science, education, products, classroom 09/21/09 12:10 PM 12/31/37 11:59 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSE /LSE/Equipment-and-Supplies/Reagents N 0 ,VN <p>Dithiothreitol (DTT), 1 g, is a reducing agent used in SDS-PAGE sample loading buffer. Dithiothreitol is a refill component for the pGLO™ Kit SDS-PAGE Extension (#166-0013EDU) and for the Comparative Proteomics Kit I: Protein Profiler Module (#166-2700EDU). This product is for education use only.</p> /webroot/web/html/lse/products/SKUs/autopopulated 161-0610EDU_0921121024180_en.html /webroot/web/html/lse/products/SKUs/autopopulated/161-0610EDU_0921121024180_en.html false $29.00 true $ %2423.20 true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1610610EDU&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1610610EDU&CartQuote=QT&quantity= 1717 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1610610EDU&AddToDefaultWishList=true&quantity= 12000000 29.00 1610610EDU

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Actin and Myosin Standard

1660010EDU
Refill kit for use with Comparative Proteomics Kit I: Protein Profiler Module (1662700EDU), includes only the actin and myosin standard; education use only

1660010EDU /templatedata/internet/product_SKU/data/LSE/Protein-Analysis-Kits/Comparative-Proteomics-Kit-I--/166-0010EDU_0921121041786.xml 6 1660010EDU /default/main/bio-rad/WORKAREA/default 09/21/09 12:10 PM en_US jthvlbie 2017-11-14 07:07:24 true true internet/product_SKU jthvlbie templatedata/internet/product_SKU/data/LSE/Protein-Analysis-Kits/Comparative-Proteomics-Kit-I--/166-0010EDU_0921121041786.xml LSE 2019-05-04 10:45:06 /default/main/bio-rad/STAGING N /default/main/bio-rad en 12/31/37 11:59 PM 1555972636222 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN 2019-05-04 10:45:06 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSE/Protein-Analysis-Kits/Comparative-Proteomics-Kit-I--/166-0010EDU_0921121041786.xml templatedata/internet/product_SKU/data/LSE/Protein-Analysis-Kits/Comparative-Proteomics-Kit-I--/166-0010EDU_0921121041786.xml 1660010edu-actin-myosin-standard 1660010EDU Actin and Myosin Standard Actin and Myosin Standard Refill kit for use with Comparative Proteomics Kit I: Protein Profiler Module (1662700EDU), includes only the actin and myosin standard; education use only Refill kit for use with Comparative Proteomics Kit I: Protein Profiler Module (#166-2700EDU), contains only the actin and myosin standard; education use only. Refill kit for use with Comparative Proteomics Kit I: Protein Profiler Module (1662700EDU), includes only the actin and myosin standard; education use only /webroot/web/images/lse/products/protein_analysis_kits/sku_thumb/global/actin-and-myosin-standard-1660010edu-tn.jpg /webroot/web/images/lse/products/protein_analysis_kits/sku_view/global/actin-and-myosin-standard-1660010edu-view.jpg ,VN RepEx 166-0010EDU Refill kit for use with Comparative Proteomics Kit I: Protein Profiler Module (#166-2700EDU), contains only the actin and myosin standard; education use only. 1660010, 1660010EDU, actin, and, myosin, standard, standards, markers, protein, gel, electrophoresis, comparative, proteomics, profiler, module, science, education, products, classroom 09/21/09 12:10 PM 12/31/37 11:59 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSE /LSE/Protein-Analysis-Kits/Comparative-Proteomics-Kit-I-- N 0 <p>The Actin and Myosin Standard is a consumable refill for use with Comparative Proteomics Kit I: Protein Profiler Module (#1662700EDU). The refill contains only the actin and myosin standard.</p> <p>The Actin and Myosin Standard is also available as part of the Protein Profiler Temperature-Sensitive Reagents Bag (#1662701EDU). This product is for education use only.</p> /webroot/web/html/lse/products/SKUs/autopopulated 166-0010EDU_0921121041786_en.html /webroot/web/html/lse/products/SKUs/autopopulated/166-0010EDU_0921121041786_en.html Actin and myosin standard, 500 µg lyophilized 1324 1 1 0 false $32.50 true $ %2426.00 true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1660010EDU&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1660010EDU&CartQuote=QT&quantity= 1711 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1660010EDU&AddToDefaultWishList=true&quantity= 41105300 32.50 1660010EDU

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Rapid Blotting + V3 Western Workflow^™ Application Note

1662876EDU
Application note for western blotting, describes rapid blotting using the Trans-Blot^® Turbo^™ Transfer System and V3 Western Workflow (stain-free blotting) methods; education use only Use Add to Cart to order a printed copy or download a free PDF.

1662876EDU /templatedata/internet/product_SKU/data/LSE/Protein-Analysis-Kits/Comparative-Proteomics-Kit-I--/166-2876EDU_1353534279.xml 1662876EDU /default/main/bio-rad/WORKAREA/default 11/21/12 01:33 PM en_US juso9kj5 2017-11-14 07:07:24 true true internet/product_SKU juso9kj5 templatedata/internet/product_SKU/data/LSE/Protein-Analysis-Kits/Comparative-Proteomics-Kit-I--/166-2876EDU_1353534279.xml LSE 2019-05-04 10:45:04 /default/main/bio-rad/STAGING N /default/main/bio-rad en 11/21/22 01:39 PM 1555972636390 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN 2019-05-04 10:45:04 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSE/Protein-Analysis-Kits/Comparative-Proteomics-Kit-I--/166-2876EDU_1353534279.xml templatedata/internet/product_SKU/data/LSE/Protein-Analysis-Kits/Comparative-Proteomics-Kit-I--/166-2876EDU_1353534279.xml 1662876edu-rapid-blotting-v3-western-workflow-application-note 1662876EDU Rapid Blotting+V3 Western Workflow Application Note Rapid Blotting + V3 Western Workflow<sup>™</sup> Application Note Application note for western blotting, describes rapid blotting using the Trans-Blot<sup>®</sup> Turbo<sup>™</sup> Transfer System and V3 Western Workflow (stain-free blotting) methods; education use only Use Add to Cart to order a printed copy or <a href="/webroot/web/pdf/lse/literature/M1662876EDU.pdf" target="_blank">download a free PDF</a>. Application note for western blotting, describes rapid blotting using the Trans-Blot<sup>®</sup> Turbo<sup>™</sup> Transfer System and V3 Western Workflow (stain-free blotting) methods; education use only Use Add to Cart to order a printed copy or <a href="//webroot/web/pdf/lse/literature/M1662876EDU.pdf" target="_blank">download a free PDF</a>. /webroot/web/images/lse/products/protein_analysis_kits/sku_thumb/global/166-2876EDU_tn.jpg /webroot/web/images/lse/products/protein_analysis_kits/sku_view/global/166-2876EDU_view.jpg /webroot/web/images/lse/products/protein_analysis_kits/sku_feature/global/166-2876EDU_feature.jpg ,VN Caryn Eaton Rapid Blotting + V3 Western Workflow<sup>™></sup> Application Note Application note for western blotting, describes rapid blotting using the Trans-Blot<sup>®</sup> Turbo<sup>™</sup> transfer system and V3 Western Workflow (stain-free blotting) methods; education use only rapid blotting, v3 western, workflow 11/21/12 01:33 PM 11/21/22 01:39 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSE /LSE/Protein-Analysis-Kits/Comparative-Proteomics-Kit-I-- N 0 <p><strong>Western blotting in less than 2 hours!</strong> This application note describes two methods that allow you to complete the entire western blot workflow in less than 2–5 hours, depending on which time-saving steps you incorporate.</p> <ul> <li>Rapid blotting using traditional SDS-PAGE technology coupled with the Trans-Blot<sup>®</sup> Turbo<sup>™</sup> transfer system</li> <li>V3 Western Workflow (stain-free blotting), which incorporates stain-free technology into the rapid blotting protocol </li> </ul> /webroot/web/html/lse/products/SKUs/autopopulated 166-2876EDU_1353534279_XX_en.xml /webroot/web/html/lse/products/SKUs/autopopulated/166-2876EDU_1353534279_XX_en.xml 1 0 false $18.75 true $ %2415.00 true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1662876EDU&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1662876EDU&CartQuote=QT&quantity= 1711 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1662876EDU&AddToDefaultWishList=true&quantity= 55101500 18.75 1662876EDU

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Number	Description	Options
6672	Map of AP-Aligned Bio-Rad Kits with Essential Knowledge	Click to download
6673	Map of AP-Aligned Bio-Rad Kits with Investigative Laboratories	Click to download
6605	Map of AP-Aligned Bio-Rad Kits with Learning Objectives	Click to download
6582	20 Questions to Master Inquiry - Protein Profiler Kit, Rev A	Click to download
M1662876EDU	Bio-Rad Explorer™ Rapid Blotting + V3 Western Workflow (Stain-Free Rapid Blotting) Application Note, Rev A	Click to download
5464	Instruction Manual, Comparative Proteomics Kit I: Protein Profiler Module Quick Guide, Bio-Rad Explorer™, Rev A	Click to download
10004530	Instruction Manual, Comparative Proteomics Kit I: Protein Profiler Module, Bio-Rad Explorer Rev F	Please login to download this document
6916	Fast Protein and Nucleic Acid Electrophoresis Flier	Click to download
6933	Fast Electrophoresis and Western Blotting Flier	Click to download

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Where can I find the lot or control number?

The lot number or control number (one or the other, not both) is printed on your product label. See the sample label below for the location of this information.

Why are Certificates of Analysis not on the Documents tab?

Certificates of Analysis are associated not only with a product, but also with a specific lot of that product. For a given product, there may be several Certificates of Analysis, especially if it is an older product line and has had several lots produced over the years. Using the Certificate of Analysis search tool, you can enter the catalog number and lot/control number for the specific product you have on hand and download the exact Certificate of Analysis you need.

I have a batch number, not a lot/control number. How do I find the Certificate of Analysis for my item?

You can use the batch number in place of the lot/control number. Use the Certificate of Analysis search tool, entering the catalog number as usual and the batch number where the lot/control number would go.

Can I get a Certificate of Analysis even if my item is expired?

Yes. While we do periodically remove Certificates of Analysis for site maintenance purposes, we typically keep them available well beyond a product's expiration date.

Why does it say "Product Name Not Found" for a Certificate of Analysis listed in the search results?

There are Certificates of Analysis that correspond to products that are either discontinued or simply not available on the website. In these situations, the Certificate of Analysis is available for download, but other product details, such as the product name, are not.

Why does it say "Not Available" for a Certificate of Analysis listed in the search results?

In this case, you have entered a valid catalog number and lot/control number and we have located the Certificate of Analysis. However, for some reason, the actual file itself is unavailable.

Please use the Request Form here or contact your local Bio-Rad Representative so that we can send you the Certificate of Analysis.

Find a Certificate of Analysis

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Comparative Proteomics Kit I: Protein Profiler Module

Overview

Comparative Proteomics Kit I: Protein Profiler Module

Comparative Proteomics Kit I: Protein Profiler Module

Comparative Proteomics Kits I and II: Protein Profiler Module and Western Blot Module

Rapid Blotting and V3 Western Workflow™ Starter Kit

4–20% Mini-PROTEAN^® TGX™ Precast Gel

Protein Profiler Temperature Sensitive Reagents Bag

2x Laemmli Sample Buffer

Precision Plus Protein^™ Kaleidoscope^™ Standards

Dithiothreitol

Actin and Myosin Standard

Rapid Blotting + V3 Western Workflow^™ Application Note

I cannot find the Certificate of Analysis I am looking for. Help!

Where can I find the catalog number, SKU number, or product number?

Where can I find the lot or control number?

Why are Certificates of Analysis not on the Documents tab?

I have a batch number, not a lot/control number. How do I find the Certificate of Analysis for my item?

Can I get a Certificate of Analysis even if my item is expired?

Why does it say "Product Name Not Found" for a Certificate of Analysis listed in the search results?

Why does it say "Not Available" for a Certificate of Analysis listed in the search results?

Support

Documents

Ordering

Our Products

Company

Welcome to Bio-Rad.com

The Americas

Europe, Middle East, Africa

$(document).ready(function(){if ($.browser.msie) {$('.cornertopright').css({"background-position":"-475px -1616px"});} else { $('.cornertopright').css({"background-position":"-479px -1616px"});}});Asia Pacific

Comparative Proteomics Kit I: Protein Profiler Module

Overview

Comparative Proteomics Kit I: Protein Profiler Module

I cannot find the Certificate of Analysis I am looking for. Help!

Where can I find the catalog number, SKU number, or product number?

Where can I find the lot or control number?

Why are Certificates of Analysis not on the Documents tab?

I have a batch number, not a lot/control number. How do I find the Certificate of Analysis for my item?

Can I get a Certificate of Analysis even if my item is expired?

Why does it say "Product Name Not Found" for a Certificate of Analysis listed in the search results?

Why does it say "Not Available" for a Certificate of Analysis listed in the search results?

Support

Documents

Ordering

Our Products

Company

Asia Pacific