The Comparative Proteomics I Kit: Protein Profiler Module guides students through the thought processes involved in a laboratory-based scientific investigation. Students make predictions about their results in pre-lab activities using Internet databases and published phylogenetic information. They then employ protein electrophoresis, the most widely used technique in life science research, to study protein structure and function, generating protein profiles from the muscles of both distantly and closely related species of fish. From their results, they compare the different species' profiles, construct cladograms (phylogenetic trees), and assign each organism a branch. Students can decide whether their results support their predictions.
Features and Benefits
With this kit, students are able to:
- Explore evolution
- Study protein structure and function
- Use polyacrylamide gel electrophoresis (PAGE) to separate proteins by size
- Construct cladograms using data from their gel analysis
- Aligns with AP Biology Big Idea 1
- Three-session laboratory activity, 45 min per session
- Provides sufficient materials for eight student workstations, up to four students per workstation
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