Comparative Proteomics Kit I: Protein Profiler Module

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Comparative Proteomics Kit I: Protein Profiler Module

Overview

Lab Preparation Checklist
Kit contains sufficient materials for 8 student workstations. (2–4 students per workstation)
Laemmli sample buffer
30 ml
Precision Plus Protein™ Kaleidoscope™ Prestained Standards
50 µl
10x Tris-glycine-SDS electrophoresis buffer
1 L
Bio-Safe™ Coomassie Stain for proteins
2 x 100 ml
Actin and myosin standard, lyophilized 500 µg
Dithiothreitol (DTT)
0.3 g
Prot/Elect™ Pipet Tips for gel loading 1 rack
1.5 ml fliptop micro test tube 60
1.5 ml screwcap micro test tube 50
Disposable plastic transfer pipets 30
Curriculum including Teacher's Guide, Student Manual, and graphic Quick Guide 1
Required Accessories Not Included in Kit:  
Fish samples 5–8 types
Adjustable micropipets, 2–20 µl 8
Power supplies 2–4
Water bath 1
Gel-staining trays 4
Foam micro test tube racks 8
If using polyacrylamide gel electrophoresis:
Required Accessories:
 
Vertical gel electrophoresis chambers 2–4
Mini-PROTEAN® TGX™ Precast Polyacrylamide Gels, 4–20%, 10-well 8
If using agarose gel electrophoresis:
Required Accessories
 
Horizontal gel electrophoresis chambers 4–8
Low-melt agarose
25 g
Pipet tips, 2–20 µl, TBR-35  
Acetic acid
100 ml
Reagent alcohol/ethanol
400 ml
Recommended (Optional) Accessories:  
Sample loading guides  
GelAir™ Drying System  
Protein Profiler Flowchart
Protein Profiler Flowchart
Protein Profiler Curriculum Fit
Protein Profiler Curriculum Fit
Theory of Evolution

This kit guides students through the thought processes involved in a laboratory-based scientific investigation. Students are asked: Can molecular evidence support the theory of evolution? Why or why not? What explanations can you suggest?

Changes in proteins can reflect changes in the gene pool. Actin and myosin are the major muscle proteins essential for locomotion and survival in all animals. Muscle consists mainly of actin and myosin, but numerous other proteins also comprise muscle tissue. The structures and functions of actin and myosin have remained relatively stable or "conserved" in all animals over evolutionary time. However, other muscle proteins exhibit considerable variation even among closely related species. Detectable variations between organisms' protein profiles reflect physiological adaptations to different environments, but they originate as random DNA mutations. Such mutation events, if favorable, persist through the natural selection process and contribute to the evolution of species — with new specialized functions.

Mutation > Variation > Specialization > Speciation = Evolution

This is an open-ended inquiry-based kit. Students make predictions about their results in prelab activities using internet databases and published phylogenetic information. They generate novel results and apply their findings directly to the problem of solving evolutionary relationships by constructing cladograms (phylogenetic trees). From their gel data, they build up a tree and assign each organism a branch. Students can decide whether their results support their predictions.

Protein Profiler Gel

Protein Profiler Gel

PowerPoint Presentation for Workshops and for Teaching

Comparative Proteomics Kit I: Protein Profiler Module

This PowerPoint presentation is intended for educational use only. The presentation provides educators with background on the Comparative Proteomics Kit I: Protein Profiler Module and includes instructions for using the kit.

Comparative Proteomics Kit I: Protein Profiler Module PowerPoint presentation

Comparative Proteomics Kit I: Protein Profiler Module

This module allows students to employ protein electrophoresis, the most widely used technique in life science research, to study protein structure and function.
Read full description

Ordering Information


  • Description
  • Specifications
  • Ordering
  • Refills
  • Documents

Description

The Comparative Proteomics I Kit: Protein Profiler Module guides students through the thought processes involved in a laboratory-based scientific investigation. Students make predictions about their results in pre-lab activities using Internet databases and published phylogenetic information. They then employ protein electrophoresis, the most widely used technique in life science research, to study protein structure and function, generating protein profiles from the muscles of both distantly and closely related species of fish. From their results, they compare the different species' profiles, construct cladograms (phylogenetic trees), and assign each organism a branch. Students can decide whether their results support their predictions.

Features and Benefits

With this kit, students are able to:

  • Explore evolution
  • Study protein structure and function
  • Use polyacrylamide gel electrophoresis (PAGE) to separate proteins by size
  • Construct cladograms using data from their gel analysis

More Information

  • Aligns with AP Biology Big Idea 1
  • Three-session laboratory activity, 45 min per session
  • Provides sufficient materials for eight student workstations, up to four students per workstation

Specifications

Kit contains sufficient materials for 8 student workstations (2–4 students per workstation)  
Laemmli sample buffer 30 ml
Kaleidoscope™ Prestained Protein Standards 50 µl
10x Tris-Glycine-SDS electrophoresis buffer 1 L
Bio-Safe™ Coomassie Stain for proteins 2 x 100 ml
Actin and myosin standard, lyophilized 500 µg
Dithiothreitol (DTT) 0.3 g
Prot/Elect™ Pipet Tips for gel loading, 1–200 µl 1 rack of 96
1.5 ml fliptop micro test tube 60
1.5 ml screwcap micro test tube 50
Disposable plastic transfer pipets 30
Curriculum, including Teacher's Guide, Student Manual, and graphic Quick Guide 1

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Comparative Proteomics Kit I: Protein Profiler Module

1662700EDU
Protein profiling classroom study kit, includes buffers, standards, stains, DTT, test tubes/holders, disposable pipets/tips, trays, curriculum, and more, for 32 students; education use only

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Comparative Proteomics Kits I and II: Protein Profiler Module and Western Blot Module

1662850EDU
Comprehensive protein classroom study kit, includes Protein Profiler Module (1662700EDU) and Western Blot Module (1662800EDU), for 32 students; education use only

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Rapid Blotting and V3 Western Workflow Starter Kit

1662875EDU
Comprehensive protein classroom study kit, includes Protein Profiler Module (1662700EDU), Western Blot Module (1662800EDU), Trans-Blot® Turbo Mini Nitrocellulose Transfer Packs (10), 4–20% Mini-PROTEAN® TGX Stain-Free Gels (10), application note, for 32 students; education use only

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Comparative Proteomics Kit and Stain-Free TGX Gels Combo

17006135EDU
Comparative Proteomics Kit and Stain-Free TGX Gels Combo, includes Comparative Proteomics Kit I: Protein Profiler Module (1662700EDU) and a pack of 10, 4-20% MP TGX Stain-Free Precast Gels (4568093EDU)

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Comparative Proteomics Kit and TGX Gels Combo

17006136EDU
Comparative Proteomics Kit and TGX Gels Combo, includes Comparative Proteomics Kit I: Protein Profiler Module (1662700EDU) and a pack of 10, 4-20% TGX Precast Gels (4561093EDU)

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$341.50 
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Refills

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list price. If the problem persists, please call customer service at or send an
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4–20% Mini-PROTEAN® TGX™ Precast Gel

4561093EDU
Pkg of 10, 4–20% precast polyacrylamide gel, 10-well, 30 µl, for use with Mini-PROTEAN Electrophoresis Cells; education use only

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$123.00 
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$98.40  
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Protein Profiler Temperature Sensitive Reagents Bag

1662701EDU
Consumables refill kit for use with Comparative Proteomics Kit I: Protein Profiler Module (1662700EDU), includes standards, actin and myosin standard, DTT; education use only

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2x Laemmli Sample Buffer

1610737EDU
30 ml, premixed protein sample buffer for SDS-PAGE; education use only

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$14.00 
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Precision Plus Protein Kaleidoscope Standards

1610375EDU
500 µl, mixture of 10 multicolor recombinant proteins (10–250 kD), including three bands (25, 50, 75 kD) that match the reference bands of other standards, 50 applications; education use only

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Dithiothreitol

1610610EDU
1 g, dithiothreitol (DTT) reducing agent; education use only

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$31.00 
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$24.80  
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Actin and Myosin Standard

1660010EDU
Refill kit for use with Comparative Proteomics Kit I: Protein Profiler Module (1662700EDU), includes only the actin and myosin standard; education use only

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Rapid Blotting + V3 Western Workflow Application Note

1662876EDU
Application note for western blotting, describes rapid blotting using the Trans-Blot® Turbo Transfer System and V3 Western Workflow (stain-free blotting) methods; education use only Use Add to Cart to order a printed copy or download a free PDF.

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4–20% Mini-PROTEAN® TGX Stain-Free™ Gel

4568093EDU
Pkg of 10, 4–20% precast polyacrylamide gel, 10-well, 30 µl, for use with Mini-PROTEAN electrophoresis cells and stain-free enabled imagers; education use only

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$136.00 
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Documents

Number Description Options
6672 Map of AP-Aligned Bio-Rad Kits with Essential Knowledge Click to download
6673 Map of AP-Aligned Bio-Rad Kits with Investigative Laboratories Click to download
6605 Map of AP-Aligned Bio-Rad Kits with Learning Objectives Click to download
6582 20 Questions to Master Inquiry - Protein Profiler Kit, Rev A Click to download
M1662876EDU Bio-Rad Explorer™ Rapid Blotting + V3 Western Workflow (Stain-Free Rapid Blotting) Application Note, Rev A Click to download
5464 Instruction Manual, Comparative Proteomics Kit I: Protein Profiler Module Quick Guide, Bio-Rad Explorer™, Rev A Click to download
10004530 Instruction Manual, Comparative Proteomics Kit I: Protein Profiler Module, Bio-Rad Explorer Rev F Please login to download this document
6916 Fast Protein and Nucleic Acid Electrophoresis Flier Click to download
6933 Fast Electrophoresis and Western Blotting Flier Click to download

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