Find the best protein standards for SDS-PAGE, western blotting, 2-D electrophoresis, and other applications.
Prestained standards enable quick visual assessment during electrophoresis and transfer.
Unstained protein ladders are optimized for Coomassie and zinc staining.
Use these standards for both fluorescent visualization and colorimetric or chemiluminescent immunodetection on western blots.
IEF and 2-D standards allow dependable and reproducible pI and MW calibration in native polyacrylamide or agarose gels.
Precision Plus Protein™Dual Color Standards
Precision Plus Protein™ Standards have the same gel migration patterns, with three high-intensity reference bands (25, 50, and 75 kD), and provide exceptional lot-to-lot molecular weight consistency.
Prestained natural protein standards for SDS-PAGE and western blotting provide a quick and easy way to monitor protein separation during electrophoresis and to assess transfer efficiency on blots. Each lot of prestained protein standards is individually calibrated for estimating the MW of sample proteins.
Important InformationPrestained Natural Protein Standards will soon be phased out. We recommend the Precision Plus Protein Prestained Recombinant Protein Standards above as replacements.
Unstained natural protein standards allow accurate MW determination with uniform band intensities on SDS-PAGE gels stained with Coomassie Blue or zinc. Precision Plus Protein Unstained Recombinant Protein Standards are Strep-tagged, enabling immunodetection and molecular weight determination on western blots.
* Recommended for use with Bio-Rad's TGX Stain-Free™ Precast Gels and TGX Stain-Free™ FastCast™ Acrylamide handcast gels.
Western blotting protein standards can be used for both fluorescent visualization and colormetric or chemiluminescent immunodetection on western blots. Precision Plus Protein™ WesternC™ Standards are combination protein standards containing ten prestained, Strep-tagged recombinant proteins, enabling both fluorescent visualization on gels and colorimetric or chemiluminescent western blot detection with StrepTactin-AP or StrepTactin-HRP Conjugate.
IEF and 2D protein standards are a mixture of native proteins with isoelectric points (pI) ranging from 4.45 to 9.6, providing reproducible pI calibration in native PAGE or agarose IEF gels. 2-D SDS-PAGE protein standards provide calibrated references for protein pI and molecular weight in the second dimension.
* 2-D standards: use 2.5 µl per mini gel for Coomassie staining (200 applications) or 0.5–2.5 µl for silver staining (up to 1,000 applications); use 1.0–5.0 µl for full-length gels (16–20 cm) to yield 100 or up to 500 applications, respectively.IEF standards: use 5 µl per mini gel for Coomassie staining or 0.5 µl for silver staining to yield 50 or 500 applications, respectively.
Protein Standards Selection Guide For quick guidance on choosing the best standard for your application. (PDF 298 KB)
Little Book of StandardsComplete reference information for all of Bio-Rad’s protein standards and nucleic acid standards. (PDF 5.4 MB)
Protein Gel Migration Charts View banding patterns for Bio-Rad protein standards on Bio-Rad and competitor gels.
Electrophoresis GuideTheory and techniques, Bio-Rad products, tips and troubleshooting. (PDF 6.4 MB)
Protein Blotting Guide Details on blotting technology, available products, and tips and techniques.(PDF 7.8 MB)
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